Bacillus sp. SRB_336

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7157 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A328LLB1|A0A328LLB1_9BACI Bifunctional protein FolD OS=Bacillus sp. SRB_336 OX=1969380 GN=folD PE=3 SV=1
MM1 pKa = 7.04SQSSTEE7 pKa = 3.98AVVLRR12 pKa = 11.84KK13 pKa = 9.1WMCVVCGYY21 pKa = 10.36IYY23 pKa = 10.73DD24 pKa = 4.04EE25 pKa = 4.56ALGVPDD31 pKa = 4.65EE32 pKa = 5.24GIAPGTRR39 pKa = 11.84WEE41 pKa = 4.7DD42 pKa = 3.78VPDD45 pKa = 3.14TWTCPDD51 pKa = 4.11CGTTKK56 pKa = 11.01DD57 pKa = 3.83DD58 pKa = 4.28FEE60 pKa = 4.9MIEE63 pKa = 4.2LDD65 pKa = 3.4

Molecular weight: 7.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A328LPF9|A0A328LPF9_9BACI PKS_ER domain-containing protein OS=Bacillus sp. SRB_336 OX=1969380 GN=B5P43_16410 PE=4 SV=1
MM1 pKa = 7.54ARR3 pKa = 11.84RR4 pKa = 11.84SRR6 pKa = 11.84PAPLPRR12 pKa = 11.84ARR14 pKa = 11.84LRR16 pKa = 11.84GTGLPKK22 pKa = 10.02PRR24 pKa = 11.84LRR26 pKa = 11.84KK27 pKa = 9.39SRR29 pKa = 11.84RR30 pKa = 11.84QRR32 pKa = 11.84AGPRR36 pKa = 11.84RR37 pKa = 11.84CPRR40 pKa = 11.84SVPPSGPGSRR50 pKa = 11.84QSRR53 pKa = 11.84VSGWRR58 pKa = 11.84PARR61 pKa = 11.84CRR63 pKa = 11.84RR64 pKa = 11.84VRR66 pKa = 11.84RR67 pKa = 11.84RR68 pKa = 11.84RR69 pKa = 11.84RR70 pKa = 11.84RR71 pKa = 11.84RR72 pKa = 11.84HH73 pKa = 5.42PPAARR78 pKa = 11.84PGRR81 pKa = 11.84GGRR84 pKa = 11.84PGGAGRR90 pKa = 11.84GPRR93 pKa = 11.84AATT96 pKa = 3.38

Molecular weight: 10.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7157

0

7157

2367332

30

2880

330.8

35.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.17 ± 0.041

0.745 ± 0.007

5.675 ± 0.025

4.984 ± 0.026

3.31 ± 0.016

8.913 ± 0.034

2.348 ± 0.015

4.235 ± 0.021

2.811 ± 0.022

10.6 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.218 ± 0.011

2.56 ± 0.017

5.386 ± 0.02

3.471 ± 0.019

6.869 ± 0.029

5.599 ± 0.022

5.447 ± 0.026

7.911 ± 0.028

1.489 ± 0.013

2.261 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski