Amino acid dipepetide frequency for Bacillus sp. SRB_336

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.967AlaAla: 19.967 ± 0.141
1.042AlaCys: 1.042 ± 0.022
7.132AlaAsp: 7.132 ± 0.058
6.815AlaGlu: 6.815 ± 0.069
3.923AlaPhe: 3.923 ± 0.041
12.612AlaGly: 12.612 ± 0.087
2.717AlaHis: 2.717 ± 0.04
5.276AlaIle: 5.276 ± 0.054
3.786AlaLys: 3.786 ± 0.047
14.263AlaLeu: 14.263 ± 0.094
3.286AlaMet: 3.286 ± 0.037
3.006AlaAsn: 3.006 ± 0.036
6.304AlaPro: 6.304 ± 0.076
4.72AlaGln: 4.72 ± 0.052
8.595AlaArg: 8.595 ± 0.074
6.993AlaSer: 6.993 ± 0.061
6.711AlaThr: 6.711 ± 0.058
10.269AlaVal: 10.269 ± 0.064
1.852AlaTrp: 1.852 ± 0.032
2.431AlaTyr: 2.431 ± 0.034
0.0AlaXaa: 0.0 ± 0.0
Cys
0.932CysAla: 0.932 ± 0.023
0.101CysCys: 0.101 ± 0.006
0.421CysAsp: 0.421 ± 0.014
0.359CysGlu: 0.359 ± 0.012
0.266CysPhe: 0.266 ± 0.012
0.818CysGly: 0.818 ± 0.02
0.216CysHis: 0.216 ± 0.009
0.299CysIle: 0.299 ± 0.013
0.156CysLys: 0.156 ± 0.009
0.702CysLeu: 0.702 ± 0.018
0.159CysMet: 0.159 ± 0.008
0.178CysAsn: 0.178 ± 0.008
0.383CysPro: 0.383 ± 0.014
0.21CysGln: 0.21 ± 0.011
0.519CysArg: 0.519 ± 0.015
0.445CysSer: 0.445 ± 0.015
0.425CysThr: 0.425 ± 0.016
0.571CysVal: 0.571 ± 0.016
0.112CysTrp: 0.112 ± 0.008
0.174CysTyr: 0.174 ± 0.007
0.0CysXaa: 0.0 ± 0.0
Asp
7.884AspAla: 7.884 ± 0.064
0.353AspCys: 0.353 ± 0.012
3.319AspAsp: 3.319 ± 0.042
3.031AspGlu: 3.031 ± 0.041
2.012AspPhe: 2.012 ± 0.027
5.801AspGly: 5.801 ± 0.054
1.274AspHis: 1.274 ± 0.025
2.385AspIle: 2.385 ± 0.044
1.488AspLys: 1.488 ± 0.028
5.421AspLeu: 5.421 ± 0.049
1.118AspMet: 1.118 ± 0.022
1.311AspAsn: 1.311 ± 0.029
3.49AspPro: 3.49 ± 0.044
1.692AspGln: 1.692 ± 0.032
3.527AspArg: 3.527 ± 0.041
2.72AspSer: 2.72 ± 0.036
2.789AspThr: 2.789 ± 0.036
4.654AspVal: 4.654 ± 0.044
0.956AspTrp: 0.956 ± 0.022
1.522AspTyr: 1.522 ± 0.029
0.0AspXaa: 0.0 ± 0.0
Glu
6.121GluAla: 6.121 ± 0.066
0.315GluCys: 0.315 ± 0.012
2.328GluAsp: 2.328 ± 0.038
2.384GluGlu: 2.384 ± 0.036
1.612GluPhe: 1.612 ± 0.027
3.543GluGly: 3.543 ± 0.051
1.463GluHis: 1.463 ± 0.025
2.306GluIle: 2.306 ± 0.035
1.584GluLys: 1.584 ± 0.032
6.246GluLeu: 6.246 ± 0.065
1.024GluMet: 1.024 ± 0.018
1.259GluAsn: 1.259 ± 0.02
2.586GluPro: 2.586 ± 0.033
2.335GluGln: 2.335 ± 0.035
4.127GluArg: 4.127 ± 0.05
2.635GluSer: 2.635 ± 0.042
2.537GluThr: 2.537 ± 0.035
3.734GluVal: 3.734 ± 0.045
0.681GluTrp: 0.681 ± 0.017
1.018GluTyr: 1.018 ± 0.023
0.0GluXaa: 0.0 ± 0.0
Phe
4.266PheAla: 4.266 ± 0.049
0.292PheCys: 0.292 ± 0.01
2.304PheAsp: 2.304 ± 0.031
1.71PheGlu: 1.71 ± 0.025
1.187PhePhe: 1.187 ± 0.026
3.406PheGly: 3.406 ± 0.035
0.766PheHis: 0.766 ± 0.019
1.364PheIle: 1.364 ± 0.024
0.828PheLys: 0.828 ± 0.02
2.98PheLeu: 2.98 ± 0.037
0.683PheMet: 0.683 ± 0.019
1.03PheAsn: 1.03 ± 0.021
1.502PhePro: 1.502 ± 0.029
0.915PheGln: 0.915 ± 0.02
1.934PheArg: 1.934 ± 0.03
2.04PheSer: 2.04 ± 0.027
2.039PheThr: 2.039 ± 0.034
2.563PheVal: 2.563 ± 0.036
0.485PheTrp: 0.485 ± 0.016
0.808PheTyr: 0.808 ± 0.019
0.0PheXaa: 0.0 ± 0.0
Gly
10.094GlyAla: 10.094 ± 0.086
0.753GlyCys: 0.753 ± 0.019
4.556GlyAsp: 4.556 ± 0.041
4.344GlyGlu: 4.344 ± 0.04
3.271GlyPhe: 3.271 ± 0.041
7.652GlyGly: 7.652 ± 0.071
2.237GlyHis: 2.237 ± 0.036
4.286GlyIle: 4.286 ± 0.046
3.067GlyLys: 3.067 ± 0.045
9.018GlyLeu: 9.018 ± 0.072
2.337GlyMet: 2.337 ± 0.035
2.477GlyAsn: 2.477 ± 0.039
3.793GlyPro: 3.793 ± 0.043
3.155GlyGln: 3.155 ± 0.04
6.083GlyArg: 6.083 ± 0.055
5.458GlySer: 5.458 ± 0.059
5.476GlyThr: 5.476 ± 0.063
6.963GlyVal: 6.963 ± 0.063
1.689GlyTrp: 1.689 ± 0.029
2.42GlyTyr: 2.42 ± 0.032
0.0GlyXaa: 0.0 ± 0.0
His
3.02HisAla: 3.02 ± 0.041
0.21HisCys: 0.21 ± 0.01
1.442HisAsp: 1.442 ± 0.023
1.225HisGlu: 1.225 ± 0.025
0.859HisPhe: 0.859 ± 0.018
2.409HisGly: 2.409 ± 0.034
0.664HisHis: 0.664 ± 0.018
0.868HisIle: 0.868 ± 0.016
0.522HisLys: 0.522 ± 0.015
2.217HisLeu: 2.217 ± 0.031
0.47HisMet: 0.47 ± 0.013
0.508HisAsn: 0.508 ± 0.015
1.55HisPro: 1.55 ± 0.034
0.722HisGln: 0.722 ± 0.015
1.658HisArg: 1.658 ± 0.029
1.184HisSer: 1.184 ± 0.022
1.123HisThr: 1.123 ± 0.021
1.772HisVal: 1.772 ± 0.026
0.399HisTrp: 0.399 ± 0.014
0.664HisTyr: 0.664 ± 0.019
0.0HisXaa: 0.0 ± 0.0
Ile
5.902IleAla: 5.902 ± 0.053
0.346IleCys: 0.346 ± 0.012
3.053IleAsp: 3.053 ± 0.041
2.543IleGlu: 2.543 ± 0.038
1.308IlePhe: 1.308 ± 0.028
4.278IleGly: 4.278 ± 0.048
0.935IleHis: 0.935 ± 0.02
1.644IleIle: 1.644 ± 0.033
1.192IleLys: 1.192 ± 0.026
3.398IleLeu: 3.398 ± 0.044
0.731IleMet: 0.731 ± 0.02
1.358IleAsn: 1.358 ± 0.026
2.185IlePro: 2.185 ± 0.031
1.107IleGln: 1.107 ± 0.025
2.504IleArg: 2.504 ± 0.036
2.454IleSer: 2.454 ± 0.038
2.482IleThr: 2.482 ± 0.038
3.549IleVal: 3.549 ± 0.035
0.459IleTrp: 0.459 ± 0.016
0.922IleTyr: 0.922 ± 0.017
0.0IleXaa: 0.0 ± 0.0
Lys
3.579LysAla: 3.579 ± 0.049
0.13LysCys: 0.13 ± 0.008
1.592LysAsp: 1.592 ± 0.031
1.237LysGlu: 1.237 ± 0.028
0.815LysPhe: 0.815 ± 0.019
2.037LysGly: 2.037 ± 0.032
0.656LysHis: 0.656 ± 0.016
1.232LysIle: 1.232 ± 0.023
1.048LysLys: 1.048 ± 0.028
3.049LysLeu: 3.049 ± 0.046
0.644LysMet: 0.644 ± 0.019
0.787LysAsn: 0.787 ± 0.021
1.799LysPro: 1.799 ± 0.029
1.056LysGln: 1.056 ± 0.023
1.892LysArg: 1.892 ± 0.03
1.625LysSer: 1.625 ± 0.029
1.609LysThr: 1.609 ± 0.033
2.335LysVal: 2.335 ± 0.034
0.318LysTrp: 0.318 ± 0.014
0.668LysTyr: 0.668 ± 0.017
0.0LysXaa: 0.0 ± 0.0
Leu
15.311LeuAla: 15.311 ± 0.094
0.845LeuCys: 0.845 ± 0.019
6.498LeuAsp: 6.498 ± 0.055
5.006LeuGlu: 5.006 ± 0.058
3.243LeuPhe: 3.243 ± 0.045
9.256LeuGly: 9.256 ± 0.081
2.358LeuHis: 2.358 ± 0.031
4.153LeuIle: 4.153 ± 0.05
3.021LeuLys: 3.021 ± 0.04
11.581LeuLeu: 11.581 ± 0.106
2.1LeuMet: 2.1 ± 0.033
2.501LeuAsn: 2.501 ± 0.034
6.179LeuPro: 6.179 ± 0.049
3.471LeuGln: 3.471 ± 0.042
7.539LeuArg: 7.539 ± 0.058
5.931LeuSer: 5.931 ± 0.05
5.574LeuThr: 5.574 ± 0.045
8.036LeuVal: 8.036 ± 0.07
1.359LeuTrp: 1.359 ± 0.027
2.042LeuTyr: 2.042 ± 0.03
0.0LeuXaa: 0.0 ± 0.0
Met
2.865MetAla: 2.865 ± 0.038
0.141MetCys: 0.141 ± 0.008
1.169MetAsp: 1.169 ± 0.021
0.943MetGlu: 0.943 ± 0.02
0.666MetPhe: 0.666 ± 0.02
1.674MetGly: 1.674 ± 0.031
0.524MetHis: 0.524 ± 0.014
0.901MetIle: 0.901 ± 0.021
0.739MetLys: 0.739 ± 0.018
2.391MetLeu: 2.391 ± 0.031
0.438MetMet: 0.438 ± 0.015
0.706MetAsn: 0.706 ± 0.017
1.37MetPro: 1.37 ± 0.021
0.803MetGln: 0.803 ± 0.018
1.497MetArg: 1.497 ± 0.024
1.539MetSer: 1.539 ± 0.025
1.485MetThr: 1.485 ± 0.027
1.704MetVal: 1.704 ± 0.032
0.235MetTrp: 0.235 ± 0.01
0.391MetTyr: 0.391 ± 0.013
0.0MetXaa: 0.0 ± 0.0
Asn
3.22AsnAla: 3.22 ± 0.044
0.196AsnCys: 0.196 ± 0.008
1.457AsnAsp: 1.457 ± 0.027
1.149AsnGlu: 1.149 ± 0.02
0.896AsnPhe: 0.896 ± 0.02
2.482AsnGly: 2.482 ± 0.039
0.587AsnHis: 0.587 ± 0.015
1.101AsnIle: 1.101 ± 0.023
0.698AsnLys: 0.698 ± 0.019
2.539AsnLeu: 2.539 ± 0.032
0.492AsnMet: 0.492 ± 0.016
0.765AsnAsn: 0.765 ± 0.02
1.765AsnPro: 1.765 ± 0.026
0.851AsnGln: 0.851 ± 0.022
1.598AsnArg: 1.598 ± 0.026
1.293AsnSer: 1.293 ± 0.026
1.42AsnThr: 1.42 ± 0.03
1.965AsnVal: 1.965 ± 0.034
0.406AsnTrp: 0.406 ± 0.014
0.715AsnTyr: 0.715 ± 0.022
0.0AsnXaa: 0.0 ± 0.0
Pro
8.005ProAla: 8.005 ± 0.084
0.3ProCys: 0.3 ± 0.012
3.505ProAsp: 3.505 ± 0.043
3.149ProGlu: 3.149 ± 0.041
1.684ProPhe: 1.684 ± 0.023
5.251ProGly: 5.251 ± 0.047
1.177ProHis: 1.177 ± 0.024
1.846ProIle: 1.846 ± 0.031
1.34ProLys: 1.34 ± 0.026
5.262ProLeu: 5.262 ± 0.052
1.231ProMet: 1.231 ± 0.024
1.243ProAsn: 1.243 ± 0.025
2.64ProPro: 2.64 ± 0.046
1.886ProGln: 1.886 ± 0.031
3.199ProArg: 3.199 ± 0.042
3.011ProSer: 3.011 ± 0.036
2.674ProThr: 2.674 ± 0.035
4.428ProVal: 4.428 ± 0.051
0.853ProTrp: 0.853 ± 0.019
1.171ProTyr: 1.171 ± 0.024
0.0ProXaa: 0.0 ± 0.0
Gln
4.636GlnAla: 4.636 ± 0.053
0.215GlnCys: 0.215 ± 0.012
1.481GlnAsp: 1.481 ± 0.027
1.385GlnGlu: 1.385 ± 0.025
1.082GlnPhe: 1.082 ± 0.023
2.579GlnGly: 2.579 ± 0.039
0.899GlnHis: 0.899 ± 0.017
1.484GlnIle: 1.484 ± 0.025
0.916GlnLys: 0.916 ± 0.022
4.245GlnLeu: 4.245 ± 0.049
0.765GlnMet: 0.765 ± 0.019
0.756GlnAsn: 0.756 ± 0.02
2.116GlnPro: 2.116 ± 0.037
1.698GlnGln: 1.698 ± 0.031
2.972GlnArg: 2.972 ± 0.04
1.803GlnSer: 1.803 ± 0.034
1.609GlnThr: 1.609 ± 0.028
2.721GlnVal: 2.721 ± 0.036
0.598GlnTrp: 0.598 ± 0.017
0.75GlnTyr: 0.75 ± 0.016
0.0GlnXaa: 0.0 ± 0.0
Arg
7.695ArgAla: 7.695 ± 0.069
0.51ArgCys: 0.51 ± 0.016
3.852ArgAsp: 3.852 ± 0.042
3.895ArgGlu: 3.895 ± 0.041
2.395ArgPhe: 2.395 ± 0.036
5.109ArgGly: 5.109 ± 0.052
1.917ArgHis: 1.917 ± 0.031
3.365ArgIle: 3.365 ± 0.04
1.942ArgLys: 1.942 ± 0.03
7.51ArgLeu: 7.51 ± 0.068
1.72ArgMet: 1.72 ± 0.028
1.794ArgAsn: 1.794 ± 0.031
3.414ArgPro: 3.414 ± 0.045
2.671ArgGln: 2.671 ± 0.036
5.799ArgArg: 5.799 ± 0.066
3.754ArgSer: 3.754 ± 0.042
3.498ArgThr: 3.498 ± 0.041
4.863ArgVal: 4.863 ± 0.047
1.184ArgTrp: 1.184 ± 0.023
1.797ArgTyr: 1.797 ± 0.025
0.0ArgXaa: 0.0 ± 0.0
Ser
6.897SerAla: 6.897 ± 0.055
0.384SerCys: 0.384 ± 0.013
2.859SerAsp: 2.859 ± 0.033
2.474SerGlu: 2.474 ± 0.035
1.977SerPhe: 1.977 ± 0.027
5.686SerGly: 5.686 ± 0.064
1.239SerHis: 1.239 ± 0.021
2.424SerIle: 2.424 ± 0.029
1.482SerLys: 1.482 ± 0.026
5.75SerLeu: 5.75 ± 0.052
1.399SerMet: 1.399 ± 0.025
1.453SerAsn: 1.453 ± 0.029
3.019SerPro: 3.019 ± 0.038
1.75SerGln: 1.75 ± 0.029
3.773SerArg: 3.773 ± 0.041
3.398SerSer: 3.398 ± 0.042
3.321SerThr: 3.321 ± 0.044
4.336SerVal: 4.336 ± 0.052
0.926SerTrp: 0.926 ± 0.02
1.446SerTyr: 1.446 ± 0.024
0.0SerXaa: 0.0 ± 0.0
Thr
7.09ThrAla: 7.09 ± 0.077
0.365ThrCys: 0.365 ± 0.014
2.929ThrAsp: 2.929 ± 0.039
2.394ThrGlu: 2.394 ± 0.034
1.846ThrPhe: 1.846 ± 0.031
5.283ThrGly: 5.283 ± 0.059
1.184ThrHis: 1.184 ± 0.021
2.298ThrIle: 2.298 ± 0.035
1.24ThrLys: 1.24 ± 0.025
6.03ThrLeu: 6.03 ± 0.054
1.103ThrMet: 1.103 ± 0.026
1.267ThrAsn: 1.267 ± 0.025
3.526ThrPro: 3.526 ± 0.042
1.638ThrGln: 1.638 ± 0.027
3.304ThrArg: 3.304 ± 0.035
3.02ThrSer: 3.02 ± 0.036
3.158ThrThr: 3.158 ± 0.045
4.885ThrVal: 4.885 ± 0.053
0.737ThrTrp: 0.737 ± 0.019
1.169ThrTyr: 1.169 ± 0.025
0.0ThrXaa: 0.0 ± 0.0
Val
10.012ValAla: 10.012 ± 0.066
0.64ValCys: 0.64 ± 0.015
4.799ValAsp: 4.799 ± 0.044
4.177ValGlu: 4.177 ± 0.047
2.593ValPhe: 2.593 ± 0.036
6.145ValGly: 6.145 ± 0.057
1.808ValHis: 1.808 ± 0.028
3.651ValIle: 3.651 ± 0.046
2.063ValLys: 2.063 ± 0.03
8.867ValLeu: 8.867 ± 0.069
1.721ValMet: 1.721 ± 0.028
2.081ValAsn: 2.081 ± 0.035
4.318ValPro: 4.318 ± 0.043
2.482ValGln: 2.482 ± 0.037
5.166ValArg: 5.166 ± 0.043
4.525ValSer: 4.525 ± 0.045
4.514ValThr: 4.514 ± 0.045
7.029ValVal: 7.029 ± 0.071
0.953ValTrp: 0.953 ± 0.021
1.563ValTyr: 1.563 ± 0.028
0.0ValXaa: 0.0 ± 0.0
Trp
1.4TrpAla: 1.4 ± 0.024
0.129TrpCys: 0.129 ± 0.007
0.738TrpAsp: 0.738 ± 0.018
0.559TrpGlu: 0.559 ± 0.016
0.587TrpPhe: 0.587 ± 0.015
0.956TrpGly: 0.956 ± 0.021
0.426TrpHis: 0.426 ± 0.014
0.703TrpIle: 0.703 ± 0.017
0.428TrpLys: 0.428 ± 0.013
2.094TrpLeu: 2.094 ± 0.035
0.377TrpMet: 0.377 ± 0.013
0.5TrpAsn: 0.5 ± 0.014
0.747TrpPro: 0.747 ± 0.019
0.734TrpGln: 0.734 ± 0.02
1.221TrpArg: 1.221 ± 0.023
0.876TrpSer: 0.876 ± 0.019
0.806TrpThr: 0.806 ± 0.02
0.973TrpVal: 0.973 ± 0.023
0.302TrpTrp: 0.302 ± 0.013
0.333TrpTyr: 0.333 ± 0.012
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.804TyrAla: 2.804 ± 0.039
0.178TyrCys: 0.178 ± 0.009
1.313TyrAsp: 1.313 ± 0.025
1.057TyrGlu: 1.057 ± 0.021
0.882TyrPhe: 0.882 ± 0.02
2.154TyrGly: 2.154 ± 0.034
0.436TyrHis: 0.436 ± 0.014
0.763TyrIle: 0.763 ± 0.019
0.568TyrLys: 0.568 ± 0.016
2.441TyrLeu: 2.441 ± 0.031
0.381TyrMet: 0.381 ± 0.011
0.615TyrAsn: 0.615 ± 0.019
1.169TyrPro: 1.169 ± 0.024
0.817TyrGln: 0.817 ± 0.021
1.778TyrArg: 1.778 ± 0.027
1.289TyrSer: 1.289 ± 0.02
1.218TyrThr: 1.218 ± 0.026
1.757TyrVal: 1.757 ± 0.03
0.384TyrTrp: 0.384 ± 0.014
0.602TyrTyr: 0.602 ± 0.019
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 7157 proteins (2367332 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski