Sinobaca qinghaiensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Sporolactobacillaceae; Sinobaca

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3379 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A419V5Q1|A0A419V5Q1_9BACL Protein RecA OS=Sinobaca qinghaiensis OX=342944 GN=recA PE=3 SV=1
MM1 pKa = 7.37NKK3 pKa = 9.74LMQFGFISVIVISLTACSEE22 pKa = 4.14EE23 pKa = 4.22SSKK26 pKa = 10.77EE27 pKa = 3.63IPKK30 pKa = 10.51AKK32 pKa = 10.02SAEE35 pKa = 4.18TSEE38 pKa = 4.65DD39 pKa = 3.33PQGFSEE45 pKa = 4.4SSKK48 pKa = 10.72EE49 pKa = 3.78GDD51 pKa = 2.99IVTVEE56 pKa = 4.0GQITYY61 pKa = 10.77AEE63 pKa = 5.02GGTAPGEE70 pKa = 4.26LYY72 pKa = 11.0GDD74 pKa = 3.66TQYY77 pKa = 11.47TIITDD82 pKa = 3.75ANGEE86 pKa = 3.89EE87 pKa = 5.08AYY89 pKa = 10.52DD90 pKa = 3.57ITHH93 pKa = 6.54SANEE97 pKa = 4.02TLMIGDD103 pKa = 4.0DD104 pKa = 3.69VKK106 pKa = 11.07VTGEE110 pKa = 3.92YY111 pKa = 10.65LGVNTGMEE119 pKa = 4.09IPAIDD124 pKa = 3.94GSKK127 pKa = 10.65VEE129 pKa = 4.44VINYY133 pKa = 9.18NNGVVTIYY141 pKa = 11.13DD142 pKa = 3.57EE143 pKa = 5.17DD144 pKa = 4.43LEE146 pKa = 5.0SNADD150 pKa = 3.48YY151 pKa = 11.29SKK153 pKa = 11.2DD154 pKa = 3.0ITIEE158 pKa = 3.85NEE160 pKa = 3.92VFFNTEE166 pKa = 3.21AEE168 pKa = 4.24FFVDD172 pKa = 3.86SDD174 pKa = 3.58EE175 pKa = 4.35TVYY178 pKa = 10.44FAEE181 pKa = 4.58LKK183 pKa = 10.99NNGSEE188 pKa = 4.09PLDD191 pKa = 3.48ISSVSITWYY200 pKa = 10.53DD201 pKa = 3.24NDD203 pKa = 5.11DD204 pKa = 3.94NVSMVTDD211 pKa = 3.41SSIEE215 pKa = 3.86VFPTVIEE222 pKa = 4.45PGEE225 pKa = 4.09KK226 pKa = 10.45GYY228 pKa = 10.49ISYY231 pKa = 11.22SDD233 pKa = 3.39VTSGVKK239 pKa = 9.87DD240 pKa = 3.75LKK242 pKa = 10.22NTEE245 pKa = 4.54LNLTPYY251 pKa = 10.73ASYY254 pKa = 11.37SSTEE258 pKa = 3.91EE259 pKa = 3.75LKK261 pKa = 10.94VEE263 pKa = 4.11NDD265 pKa = 3.02HH266 pKa = 7.34FEE268 pKa = 4.38AKK270 pKa = 9.16TDD272 pKa = 3.39SWDD275 pKa = 3.15EE276 pKa = 4.49SYY278 pKa = 11.61VHH280 pKa = 6.79ILGEE284 pKa = 3.95ITNQSEE290 pKa = 4.5TPLYY294 pKa = 9.2NHH296 pKa = 6.32TVYY299 pKa = 11.03SVFRR303 pKa = 11.84DD304 pKa = 3.51EE305 pKa = 4.88NGNFLGLDD313 pKa = 3.64STSGDD318 pKa = 3.1TTLQPNQTSGFEE330 pKa = 3.99ISGEE334 pKa = 4.08DD335 pKa = 3.5LPKK338 pKa = 10.72EE339 pKa = 3.99QIEE342 pKa = 4.72KK343 pKa = 9.93IDD345 pKa = 3.68SVEE348 pKa = 3.79NSAYY352 pKa = 10.59ADD354 pKa = 3.59TFDD357 pKa = 3.79YY358 pKa = 11.41

Molecular weight: 39.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A419UVY3|A0A419UVY3_9BACL Fructokinase OS=Sinobaca qinghaiensis OX=342944 GN=ATL39_3232 PE=3 SV=1
MM1 pKa = 7.69GKK3 pKa = 7.98PTFNPNNRR11 pKa = 11.84KK12 pKa = 9.34RR13 pKa = 11.84KK14 pKa = 8.21KK15 pKa = 8.76NHH17 pKa = 4.93GFRR20 pKa = 11.84TRR22 pKa = 11.84MSTKK26 pKa = 9.81NGRR29 pKa = 11.84HH30 pKa = 6.38IIRR33 pKa = 11.84NRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 8.63GRR40 pKa = 11.84KK41 pKa = 8.69VLSAA45 pKa = 4.05

Molecular weight: 5.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3379

0

3379

996966

29

1530

295.0

32.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.201 ± 0.051

0.647 ± 0.012

5.151 ± 0.035

8.273 ± 0.06

4.316 ± 0.037

7.15 ± 0.044

2.176 ± 0.022

7.087 ± 0.039

5.991 ± 0.045

9.42 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.974 ± 0.021

3.777 ± 0.028

3.802 ± 0.022

3.718 ± 0.031

4.293 ± 0.032

6.243 ± 0.03

5.502 ± 0.029

6.864 ± 0.037

1.077 ± 0.016

3.338 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski