Ideonella dechloratans
Average proteome isoelectric point is 6.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4151 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A643F6X1|A0A643F6X1_IDEDE RNA-binding protein (Fragment) OS=Ideonella dechloratans OX=36863 GN=F7Q92_19920 PE=4 SV=1
MM1 pKa = 8.0 SEE3 pKa = 4.14 IASIDD8 pKa = 3.33 AGLADD13 pKa = 4.5 ARR15 pKa = 11.84 AEE17 pKa = 3.98 ATRR20 pKa = 11.84 AYY22 pKa = 9.32 IDD24 pKa = 3.45 TGAGQAAVLLYY35 pKa = 10.51 DD36 pKa = 4.14 ASNVLVASIPLANPCGSVSGGVLTLAEE63 pKa = 4.67 SGDD66 pKa = 3.79 ALALVDD72 pKa = 4.22 GTPAAAAIASANGSVCIYY90 pKa = 9.67 GLTVGAPGSGAGVEE104 pKa = 4.49 LSTATLFAGGTVRR117 pKa = 11.84 LTGGTLSS124 pKa = 3.56
Molecular weight: 11.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.77
IPC2_protein 3.846
IPC_protein 3.719
Toseland 3.528
ProMoST 3.91
Dawson 3.719
Bjellqvist 3.884
Wikipedia 3.694
Rodwell 3.554
Grimsley 3.452
Solomon 3.681
Lehninger 3.643
Nozaki 3.872
DTASelect 4.05
Thurlkill 3.605
EMBOSS 3.694
Sillero 3.846
Patrickios 0.604
IPC_peptide 3.681
IPC2_peptide 3.808
IPC2.peptide.svr19 3.774
Protein with the highest isoelectric point:
>tr|A0A643F3D5|A0A643F3D5_IDEDE Uncharacterized protein OS=Ideonella dechloratans OX=36863 GN=F7Q92_20985 PE=4 SV=1
MM1 pKa = 7.93 PPRR4 pKa = 11.84 PAPTPRR10 pKa = 11.84 HH11 pKa = 5.61 RR12 pKa = 11.84 RR13 pKa = 11.84 LALAALTALVLALHH27 pKa = 6.48 GWVLGPAWTTFALRR41 pKa = 11.84 HH42 pKa = 5.49 APAPARR48 pKa = 11.84 ALPVVTLSQVRR59 pKa = 11.84 PPPPPAAAPAPAPAPPPP76 pKa = 3.76
Molecular weight: 7.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.462
IPC2_protein 11.008
IPC_protein 12.618
Toseland 12.778
ProMoST 13.276
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.281
Grimsley 12.822
Solomon 13.276
Lehninger 13.173
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.047
IPC_peptide 13.276
IPC2_peptide 12.266
IPC2.peptide.svr19 9.153
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4151
0
4151
1362198
14
2151
328.2
35.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.402 ± 0.049
0.93 ± 0.014
5.269 ± 0.026
5.316 ± 0.034
3.171 ± 0.025
8.421 ± 0.037
2.366 ± 0.02
3.597 ± 0.029
2.771 ± 0.039
11.485 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.241 ± 0.017
2.208 ± 0.022
5.73 ± 0.03
4.447 ± 0.028
7.309 ± 0.041
5.116 ± 0.03
5.024 ± 0.028
7.477 ± 0.031
1.709 ± 0.02
2.011 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here