Rhodobacter sp. SW2

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Rhodobacter; unclassified Rhodobacter

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3405 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C8S4P7|C8S4P7_9RHOB Uncharacterized protein OS=Rhodobacter sp. SW2 OX=371731 GN=Rsw2DRAFT_3025 PE=4 SV=1
MM1 pKa = 6.9TQGLLALIMLASVLIGGTGQAQAQDD26 pKa = 4.2VNWVLSLSDD35 pKa = 3.82AGSDD39 pKa = 3.35PVAAGGTIAYY49 pKa = 7.37TMSVTNDD56 pKa = 3.29GFDD59 pKa = 3.57AAPPTSITLQIPANTRR75 pKa = 11.84FTGATGSITGCAPTPSIGPSSVTCNVPPLAPSAVATLSANLLTSVSGDD123 pKa = 3.46VFFSASVPPVGDD135 pKa = 3.61TDD137 pKa = 4.15PADD140 pKa = 3.69NDD142 pKa = 3.59LTEE145 pKa = 4.09QTTITAGADD154 pKa = 3.02VGLVLRR160 pKa = 11.84GPATASAGEE169 pKa = 4.19VISYY173 pKa = 10.62SFAATNNGPDD183 pKa = 3.62PVTDD187 pKa = 4.5LVLQFPIPSGVTNVVAPAGCVLTATTYY214 pKa = 11.25DD215 pKa = 3.63CTIPGPVAVGASVNVDD231 pKa = 3.56FTGQISAASGSTVTAIGSVGAGNPSDD257 pKa = 5.58PIDD260 pKa = 4.51SNDD263 pKa = 3.47NASLNTSVTAGSDD276 pKa = 3.17LTIAKK281 pKa = 9.65SRR283 pKa = 11.84SPSGSLLVGNPAVFTLTPSYY303 pKa = 10.67TGDD306 pKa = 3.56SPNGIVVTDD315 pKa = 4.97SIPANYY321 pKa = 9.47TIDD324 pKa = 3.61TVTAPGWICTVTGQVVEE341 pKa = 4.53CSRR344 pKa = 11.84TTGSGAGANVPLGPISIATTVATPGTPTNTTSITAAGPDD383 pKa = 3.84DD384 pKa = 4.43PVPGNNSATDD394 pKa = 3.55GGATIEE400 pKa = 4.33EE401 pKa = 4.56PVVDD405 pKa = 4.68LQANKK410 pKa = 10.33SGPFPALVVVGNSYY424 pKa = 11.56DD425 pKa = 3.81FTISTSNVGNAAFFGTLEE443 pKa = 4.18MTDD446 pKa = 3.69TLPAGLTVTAYY457 pKa = 10.49AEE459 pKa = 4.28NGWTCTPAVSVVGPASILCSRR480 pKa = 11.84VYY482 pKa = 9.82TAGAPLAAGATTPSVTLTTTATTAGSVVNTMSVLSPDD519 pKa = 3.48SNLPEE524 pKa = 5.67DD525 pKa = 4.14PLKK528 pKa = 11.24DD529 pKa = 4.33PLILDD534 pKa = 4.12NNTTTYY540 pKa = 10.96AVSSSEE546 pKa = 4.41GPDD549 pKa = 3.25SADD552 pKa = 2.74ISVIKK557 pKa = 10.59SATLASLNAGEE568 pKa = 4.47VQTFTIEE575 pKa = 4.15VVNNGPVVSTDD586 pKa = 3.52VEE588 pKa = 4.43MTDD591 pKa = 3.84NLQGLINSSVGAAGAGYY608 pKa = 10.11ISDD611 pKa = 3.99SFTTGAAGGISCSTAATGGTSRR633 pKa = 11.84EE634 pKa = 4.08LSCVIDD640 pKa = 3.64TLPICTAGVDD650 pKa = 3.87CPVFTVQVRR659 pKa = 11.84PGGDD663 pKa = 2.68GGARR667 pKa = 11.84TNTASAISQTVPDD680 pKa = 4.47SNLGNNADD688 pKa = 3.88SAPFTLAPRR697 pKa = 11.84VDD699 pKa = 3.85VAVSKK704 pKa = 11.06ADD706 pKa = 3.67SPDD709 pKa = 3.43PAAAGQNLTYY719 pKa = 10.85VITAQNLANGLSTATGVTITDD740 pKa = 3.82TLPLNVTFVSASPSSGTCGTTPPANSTTTAGSRR773 pKa = 11.84TVSCVLGSIGNGAQQTVTVIVRR795 pKa = 11.84PNLITRR801 pKa = 11.84NTTLQNDD808 pKa = 3.78VAYY811 pKa = 10.44SGAVPDD817 pKa = 4.59IEE819 pKa = 4.72TGNDD823 pKa = 3.05TASATTFVSPPRR835 pKa = 11.84IDD837 pKa = 5.64LIVNKK842 pKa = 10.05DD843 pKa = 3.61DD844 pKa = 4.05TVDD847 pKa = 3.82PLPIGDD853 pKa = 3.87DD854 pKa = 3.22TTYY857 pKa = 11.35LVTVANLGPSSAEE870 pKa = 3.71NVLVTDD876 pKa = 3.61TMPPSRR882 pKa = 11.84LTYY885 pKa = 9.48QSHH888 pKa = 5.78VATGATCNSVPALDD902 pKa = 4.94SLGQTLACTFPVIPAGEE919 pKa = 4.26SRR921 pKa = 11.84TISITARR928 pKa = 11.84GVAKK932 pKa = 10.43GVGLNSVSVSSAEE945 pKa = 3.91TALGYY950 pKa = 10.33EE951 pKa = 4.55PNTGNNSTTEE961 pKa = 3.85QTSIRR966 pKa = 11.84TRR968 pKa = 11.84ADD970 pKa = 2.98MEE972 pKa = 4.6VASKK976 pKa = 8.95TATPATVNLRR986 pKa = 11.84DD987 pKa = 3.74PFSYY991 pKa = 10.85LIVVEE996 pKa = 4.45NNTGTGLAEE1005 pKa = 4.52ADD1007 pKa = 3.72DD1008 pKa = 4.3VVVSDD1013 pKa = 4.34TLPSGMQLSGAPTVSVISGTTTTSTCTGASGSTSFTCALGTVSSGAVVHH1062 pKa = 5.18VTVPARR1068 pKa = 11.84IVTVTALGQTFTNTASVSTSSLDD1091 pKa = 3.48VLPGNNSNSGPVVANSSSLSGTVFRR1116 pKa = 11.84DD1117 pKa = 4.52FAANGTLDD1125 pKa = 3.67GSDD1128 pKa = 3.18TGIVGVTMTLTGTALDD1144 pKa = 3.89GTPVSRR1150 pKa = 11.84TVTTGAGGAYY1160 pKa = 10.17AFDD1163 pKa = 4.47FLPEE1167 pKa = 3.98GTYY1170 pKa = 10.47SISQGAISEE1179 pKa = 4.62SYY1181 pKa = 8.68LTNGATAAGPGGGTPAPTQITAIPLLPATASPGYY1215 pKa = 9.67IFPKK1219 pKa = 9.86VPQARR1224 pKa = 11.84VAIAKK1229 pKa = 9.27AVQAGPMINADD1240 pKa = 2.78GSYY1243 pKa = 10.84NVTFRR1248 pKa = 11.84LRR1250 pKa = 11.84VQNPSLEE1257 pKa = 4.03GLTNVAVTDD1266 pKa = 4.32PLEE1269 pKa = 4.37GAAPLFGTYY1278 pKa = 9.18VALAAPTVPGTYY1290 pKa = 10.0TMVAAPSGSCGGLNGGFTGAGPATVASGFAIAAGATCTIDD1330 pKa = 3.16LQLRR1334 pKa = 11.84VQPTQPLPPLTGAGRR1349 pKa = 11.84YY1350 pKa = 8.87LNQATVDD1357 pKa = 3.67AQGVLSGQTSASNPQLTDD1375 pKa = 3.33LSDD1378 pKa = 3.49NGTSADD1384 pKa = 3.44ANGNGRR1390 pKa = 11.84GNEE1393 pKa = 3.97AGEE1396 pKa = 4.14NDD1398 pKa = 4.35PTPVTPVYY1406 pKa = 10.76NPAITLTKK1414 pKa = 10.58AIDD1417 pKa = 3.93LSVLPSPIAADD1428 pKa = 3.62SPISFTFTVTNSGNITLTDD1447 pKa = 3.47VTLSDD1452 pKa = 3.81ALAGAVVTGGPITLAPGQVDD1472 pKa = 3.73STSFTATYY1480 pKa = 9.24PLSAADD1486 pKa = 3.68LTANTLSNTATATGTWGLDD1505 pKa = 3.16AGGNPQKK1512 pKa = 10.96VSDD1515 pKa = 4.77PDD1517 pKa = 3.68TVATSFADD1525 pKa = 3.21IALVKK1530 pKa = 10.55AADD1533 pKa = 3.68ASLLSVPPQVGDD1545 pKa = 3.6IISYY1549 pKa = 10.83DD1550 pKa = 3.53FAVTNTGNVPLTNVTLSDD1568 pKa = 3.43ILTDD1572 pKa = 3.59AVLTGGPIPSLAVGATDD1589 pKa = 3.8SSTFTATYY1597 pKa = 10.97ALTQADD1603 pKa = 3.42IDD1605 pKa = 3.77AGEE1608 pKa = 4.36VINRR1612 pKa = 11.84ATADD1616 pKa = 3.56GVYY1619 pKa = 9.34GTDD1622 pKa = 4.69DD1623 pKa = 3.9GGDD1626 pKa = 3.95PISVQDD1632 pKa = 3.54EE1633 pKa = 4.16SGANVTEE1640 pKa = 4.73DD1641 pKa = 3.48ADD1643 pKa = 4.19TVVPLTQAPRR1653 pKa = 11.84IEE1655 pKa = 4.85LIKK1658 pKa = 10.79SLSSLVDD1665 pKa = 3.27TTGDD1669 pKa = 3.68GQIGAGDD1676 pKa = 3.52TANYY1680 pKa = 9.57GFTVTNTGNVALAGVTVSDD1699 pKa = 4.34LLVSVSGGPVSLAIGATDD1717 pKa = 3.43STSFTAAYY1725 pKa = 10.45VLTQADD1731 pKa = 3.37VDD1733 pKa = 3.69RR1734 pKa = 11.84GYY1736 pKa = 10.97VQNTATATGAAVTSSGDD1753 pKa = 3.79PILDD1757 pKa = 3.35

Molecular weight: 174.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C8S4X4|C8S4X4_9RHOB Short-chain dehydrogenase/reductase SDR OS=Rhodobacter sp. SW2 OX=371731 GN=Rsw2DRAFT_3102 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.34GGRR28 pKa = 11.84LVLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.1GRR39 pKa = 11.84KK40 pKa = 8.83KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3405

0

3405

1062988

30

2893

312.2

33.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.909 ± 0.075

0.897 ± 0.014

5.673 ± 0.042

5.093 ± 0.036

3.576 ± 0.029

9.009 ± 0.06

1.994 ± 0.022

4.74 ± 0.03

2.869 ± 0.032

10.72 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.652 ± 0.019

2.4 ± 0.026

5.346 ± 0.037

3.262 ± 0.023

6.803 ± 0.049

4.768 ± 0.031

5.358 ± 0.039

7.48 ± 0.035

1.414 ± 0.017

2.038 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski