Berryella intestinalis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Coriobacteriia; Eggerthellales; Eggerthellaceae; Berryella

Average proteome isoelectric point is 5.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1405 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0A8B4V0|A0A0A8B4V0_9ACTN Fumarate reductase OS=Berryella intestinalis OX=1531429 GN=JI75_06660 PE=4 SV=1
MM1 pKa = 7.52ARR3 pKa = 11.84FATIKK8 pKa = 10.41IPAEE12 pKa = 3.74LFAPAEE18 pKa = 4.08SSRR21 pKa = 11.84YY22 pKa = 9.53EE23 pKa = 3.94GLFDD27 pKa = 6.11LGTLHH32 pKa = 7.14AGSDD36 pKa = 4.01EE37 pKa = 4.15YY38 pKa = 11.37RR39 pKa = 11.84FSDD42 pKa = 3.86PVAWEE47 pKa = 3.82VDD49 pKa = 3.53VTNTGEE55 pKa = 3.96ALLVSGRR62 pKa = 11.84ASGCAVVDD70 pKa = 3.95CARR73 pKa = 11.84CLDD76 pKa = 3.84EE77 pKa = 6.21VEE79 pKa = 4.8FDD81 pKa = 4.25LEE83 pKa = 4.53GEE85 pKa = 3.98IEE87 pKa = 4.78GYY89 pKa = 10.17FLIGSEE95 pKa = 3.93QDD97 pKa = 3.49GVEE100 pKa = 4.22GLDD103 pKa = 3.63DD104 pKa = 5.53DD105 pKa = 4.9EE106 pKa = 7.23FDD108 pKa = 3.91VLPANHH114 pKa = 7.07EE115 pKa = 3.99IDD117 pKa = 3.97LVPLIEE123 pKa = 4.51AALLVEE129 pKa = 4.72VPFVPLCRR137 pKa = 11.84DD138 pKa = 3.42DD139 pKa = 5.44CAGLCPNCGANLNEE153 pKa = 4.59GDD155 pKa = 4.49CGCGGDD161 pKa = 3.41AAVRR165 pKa = 11.84EE166 pKa = 4.46FDD168 pKa = 3.32EE169 pKa = 4.5AANPFSVLKK178 pKa = 10.75RR179 pKa = 11.84IDD181 pKa = 3.38LGG183 pKa = 3.75

Molecular weight:
19.55 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0A8BAL6|A0A0A8BAL6_9ACTN Uncharacterized protein OS=Berryella intestinalis OX=1531429 GN=JI75_05400 PE=4 SV=1
MM1 pKa = 7.03ATEE4 pKa = 4.23SVEE7 pKa = 4.46ALALQAFEE15 pKa = 4.02LSKK18 pKa = 11.17RR19 pKa = 11.84EE20 pKa = 4.02VLVHH24 pKa = 6.94LRR26 pKa = 11.84FLNGASNLLKK36 pKa = 10.22PLAVRR41 pKa = 11.84GSSLATDD48 pKa = 4.19GTRR51 pKa = 11.84FRR53 pKa = 11.84FDD55 pKa = 3.37PVDD58 pKa = 3.55LARR61 pKa = 11.84SYY63 pKa = 11.72ARR65 pKa = 11.84DD66 pKa = 3.58PGSVARR72 pKa = 11.84AYY74 pKa = 11.02LHH76 pKa = 6.41VLLHH80 pKa = 6.18NVFSFCTRR88 pKa = 11.84MPEE91 pKa = 3.98KK92 pKa = 10.69ALTRR96 pKa = 11.84DD97 pKa = 3.43AGTLPATSRR106 pKa = 11.84SKK108 pKa = 10.83RR109 pKa = 11.84RR110 pKa = 3.33

Molecular weight:
12.19 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1405

0

1405

476188

37

2257

338.9

36.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.692 ± 0.082

1.501 ± 0.032

6.14 ± 0.056

6.545 ± 0.058

3.889 ± 0.05

8.435 ± 0.054

1.675 ± 0.025

5.14 ± 0.05

3.814 ± 0.051

9.118 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.59 ± 0.029

2.784 ± 0.037

4.191 ± 0.04

2.713 ± 0.029

6.365 ± 0.075

6.444 ± 0.049

4.907 ± 0.045

8.351 ± 0.046

0.989 ± 0.022

2.718 ± 0.033

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski