Berryella intestinalis
Average proteome isoelectric point is 5.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1405 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A8B4V0|A0A0A8B4V0_9ACTN Fumarate reductase OS=Berryella intestinalis OX=1531429 GN=JI75_06660 PE=4 SV=1
MM1 pKa = 7.52 ARR3 pKa = 11.84 FATIKK8 pKa = 10.41 IPAEE12 pKa = 3.74 LFAPAEE18 pKa = 4.08 SSRR21 pKa = 11.84 YY22 pKa = 9.53 EE23 pKa = 3.94 GLFDD27 pKa = 6.11 LGTLHH32 pKa = 7.14 AGSDD36 pKa = 4.01 EE37 pKa = 4.15 YY38 pKa = 11.37 RR39 pKa = 11.84 FSDD42 pKa = 3.86 PVAWEE47 pKa = 3.82 VDD49 pKa = 3.53 VTNTGEE55 pKa = 3.96 ALLVSGRR62 pKa = 11.84 ASGCAVVDD70 pKa = 3.95 CARR73 pKa = 11.84 CLDD76 pKa = 3.84 EE77 pKa = 6.21 VEE79 pKa = 4.8 FDD81 pKa = 4.25 LEE83 pKa = 4.53 GEE85 pKa = 3.98 IEE87 pKa = 4.78 GYY89 pKa = 10.17 FLIGSEE95 pKa = 3.93 QDD97 pKa = 3.49 GVEE100 pKa = 4.22 GLDD103 pKa = 3.63 DD104 pKa = 5.53 DD105 pKa = 4.9 EE106 pKa = 7.23 FDD108 pKa = 3.91 VLPANHH114 pKa = 7.07 EE115 pKa = 3.99 IDD117 pKa = 3.97 LVPLIEE123 pKa = 4.51 AALLVEE129 pKa = 4.72 VPFVPLCRR137 pKa = 11.84 DD138 pKa = 3.42 DD139 pKa = 5.44 CAGLCPNCGANLNEE153 pKa = 4.59 GDD155 pKa = 4.49 CGCGGDD161 pKa = 3.41 AAVRR165 pKa = 11.84 EE166 pKa = 4.46 FDD168 pKa = 3.32 EE169 pKa = 4.5 AANPFSVLKK178 pKa = 10.75 RR179 pKa = 11.84 IDD181 pKa = 3.38 LGG183 pKa = 3.75
Molecular weight: 19.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.709
IPC2_protein 3.783
IPC_protein 3.77
Toseland 3.567
ProMoST 3.91
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.656
Rodwell 3.592
Grimsley 3.478
Solomon 3.732
Lehninger 3.694
Nozaki 3.859
DTASelect 4.05
Thurlkill 3.605
EMBOSS 3.668
Sillero 3.884
Patrickios 1.1
IPC_peptide 3.732
IPC2_peptide 3.872
IPC2.peptide.svr19 3.794
Protein with the highest isoelectric point:
>tr|A0A0A8BAL6|A0A0A8BAL6_9ACTN Uncharacterized protein OS=Berryella intestinalis OX=1531429 GN=JI75_05400 PE=4 SV=1
MM1 pKa = 7.03 ATEE4 pKa = 4.23 SVEE7 pKa = 4.46 ALALQAFEE15 pKa = 4.02 LSKK18 pKa = 11.17 RR19 pKa = 11.84 EE20 pKa = 4.02 VLVHH24 pKa = 6.94 LRR26 pKa = 11.84 FLNGASNLLKK36 pKa = 10.22 PLAVRR41 pKa = 11.84 GSSLATDD48 pKa = 4.19 GTRR51 pKa = 11.84 FRR53 pKa = 11.84 FDD55 pKa = 3.37 PVDD58 pKa = 3.55 LARR61 pKa = 11.84 SYY63 pKa = 11.72 ARR65 pKa = 11.84 DD66 pKa = 3.58 PGSVARR72 pKa = 11.84 AYY74 pKa = 11.02 LHH76 pKa = 6.41 VLLHH80 pKa = 6.18 NVFSFCTRR88 pKa = 11.84 MPEE91 pKa = 3.98 KK92 pKa = 10.69 ALTRR96 pKa = 11.84 DD97 pKa = 3.43 AGTLPATSRR106 pKa = 11.84 SKK108 pKa = 10.83 RR109 pKa = 11.84 RR110 pKa = 3.33
Molecular weight: 12.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.344
IPC2_protein 9.56
IPC_protein 10.496
Toseland 10.701
ProMoST 10.877
Dawson 10.774
Bjellqvist 10.526
Wikipedia 11.023
Rodwell 10.877
Grimsley 10.818
Solomon 10.95
Lehninger 10.906
Nozaki 10.687
DTASelect 10.511
Thurlkill 10.687
EMBOSS 11.111
Sillero 10.716
Patrickios 10.657
IPC_peptide 10.95
IPC2_peptide 9.575
IPC2.peptide.svr19 8.871
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1405
0
1405
476188
37
2257
338.9
36.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.692 ± 0.082
1.501 ± 0.032
6.14 ± 0.056
6.545 ± 0.058
3.889 ± 0.05
8.435 ± 0.054
1.675 ± 0.025
5.14 ± 0.05
3.814 ± 0.051
9.118 ± 0.073
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.59 ± 0.029
2.784 ± 0.037
4.191 ± 0.04
2.713 ± 0.029
6.365 ± 0.075
6.444 ± 0.049
4.907 ± 0.045
8.351 ± 0.046
0.989 ± 0.022
2.718 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here