Hubei tombus-like virus 11

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGV0|A0A1L3KGV0_9VIRU Uncharacterized protein OS=Hubei tombus-like virus 11 OX=1923257 PE=4 SV=1
MM1 pKa = 8.11EE2 pKa = 4.18YY3 pKa = 9.98TLNVPTRR10 pKa = 11.84GPLGGSSRR18 pKa = 11.84QVNSQYY24 pKa = 11.16SSRR27 pKa = 11.84SYY29 pKa = 10.58GYY31 pKa = 9.21YY32 pKa = 9.31RR33 pKa = 11.84RR34 pKa = 11.84KK35 pKa = 9.61AASRR39 pKa = 11.84HH40 pKa = 4.85FKK42 pKa = 10.53PIGEE46 pKa = 5.03AFDD49 pKa = 4.64EE50 pKa = 4.27MDD52 pKa = 3.75GQNIPTQHH60 pKa = 6.54LRR62 pKa = 11.84DD63 pKa = 3.54NMEE66 pKa = 4.34RR67 pKa = 11.84LDD69 pKa = 4.45AEE71 pKa = 4.44PSVQEE76 pKa = 4.16SMIAGYY82 pKa = 10.17LLPTPEE88 pKa = 4.24TFQNGHH94 pKa = 6.37IPAHH98 pKa = 6.88DD99 pKa = 4.49DD100 pKa = 3.37EE101 pKa = 5.17PLQHH105 pKa = 6.88HH106 pKa = 6.27MLEE109 pKa = 4.64KK110 pKa = 10.41FDD112 pKa = 5.47IIPGYY117 pKa = 10.67DD118 pKa = 3.09VTLNEE123 pKa = 4.41QPVEE127 pKa = 4.11NQQVMVCTEE136 pKa = 4.98LLRR139 pKa = 11.84QLQTYY144 pKa = 10.11AAFKK148 pKa = 10.37PRR150 pKa = 11.84TSEE153 pKa = 4.58LLLSLKK159 pKa = 10.86NKK161 pKa = 9.66GSQLARR167 pKa = 11.84PYY169 pKa = 11.21ANSAEE174 pKa = 4.23WKK176 pKa = 8.13HH177 pKa = 5.13QQVLMCSAIAFEE189 pKa = 4.82PSTEE193 pKa = 4.2EE194 pKa = 3.54ITALAHH200 pKa = 6.21ISSSAVATKK209 pKa = 8.28WTFVNDD215 pKa = 3.57RR216 pKa = 11.84LAGGNKK222 pKa = 9.84DD223 pKa = 5.29LIDD226 pKa = 4.08CRR228 pKa = 11.84VLKK231 pKa = 10.49PKK233 pKa = 10.38LATRR237 pKa = 11.84FLSWIYY243 pKa = 10.54PSVTKK248 pKa = 7.92QTPVPVNN255 pKa = 3.27

Molecular weight: 28.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGV0|A0A1L3KGV0_9VIRU Uncharacterized protein OS=Hubei tombus-like virus 11 OX=1923257 PE=4 SV=1
MM1 pKa = 7.67SEE3 pKa = 3.95RR4 pKa = 11.84DD5 pKa = 3.42KK6 pKa = 11.46TRR8 pKa = 11.84ATPWKK13 pKa = 9.08QYY15 pKa = 9.7CFKK18 pKa = 10.94SFPGWYY24 pKa = 9.62KK25 pKa = 10.3VDD27 pKa = 4.05YY28 pKa = 9.96PSSTYY33 pKa = 9.85IDD35 pKa = 3.8EE36 pKa = 4.44EE37 pKa = 4.32VSLRR41 pKa = 11.84NRR43 pKa = 11.84ILLPMPQPQLNTPQWLSFVRR63 pKa = 11.84EE64 pKa = 3.99MRR66 pKa = 11.84HH67 pKa = 6.14LKK69 pKa = 9.57HH70 pKa = 5.69QVPIVEE76 pKa = 4.4TLTRR80 pKa = 11.84QQVILKK86 pKa = 7.68YY87 pKa = 8.92TGAKK91 pKa = 6.64RR92 pKa = 11.84TRR94 pKa = 11.84YY95 pKa = 8.64EE96 pKa = 3.61KK97 pKa = 10.83AAISLMTKK105 pKa = 9.9PLNKK109 pKa = 8.91RR110 pKa = 11.84DD111 pKa = 3.68SYY113 pKa = 11.28IDD115 pKa = 3.63CFLKK119 pKa = 10.43VEE121 pKa = 4.31KK122 pKa = 9.93MPHH125 pKa = 4.82EE126 pKa = 4.1TLKK129 pKa = 10.7IKK131 pKa = 10.45APRR134 pKa = 11.84VIQGRR139 pKa = 11.84SPRR142 pKa = 11.84YY143 pKa = 8.28NLEE146 pKa = 3.58LMRR149 pKa = 11.84YY150 pKa = 8.46LRR152 pKa = 11.84PIEE155 pKa = 3.59HH156 pKa = 7.11WFYY159 pKa = 11.16QKK161 pKa = 11.0PEE163 pKa = 3.83ILKK166 pKa = 9.49TNSLEE171 pKa = 3.91QRR173 pKa = 11.84ALKK176 pKa = 9.91IQQLLDD182 pKa = 3.17KK183 pKa = 10.16HH184 pKa = 6.91DD185 pKa = 4.2NPTVYY190 pKa = 10.52QIDD193 pKa = 3.96CSKK196 pKa = 11.0CDD198 pKa = 3.02AHH200 pKa = 5.75TTHH203 pKa = 6.61HH204 pKa = 5.92VLRR207 pKa = 11.84LEE209 pKa = 3.9HH210 pKa = 6.82NIYY213 pKa = 10.61KK214 pKa = 10.27KK215 pKa = 10.71ASRR218 pKa = 11.84NDD220 pKa = 3.56AKK222 pKa = 10.52CSQMLDD228 pKa = 3.22WQLLNVGFTPNGWTYY243 pKa = 9.46KK244 pKa = 7.51TTGRR248 pKa = 11.84RR249 pKa = 11.84ASGDD253 pKa = 3.53FNTGLGTSIICRR265 pKa = 11.84SLLKK269 pKa = 10.87GCIKK273 pKa = 9.39YY274 pKa = 10.15HH275 pKa = 6.28RR276 pKa = 11.84LRR278 pKa = 11.84ASFVCDD284 pKa = 4.04GDD286 pKa = 3.89DD287 pKa = 4.55CMLITSGKK295 pKa = 7.7LTRR298 pKa = 11.84AQIEE302 pKa = 4.18QVRR305 pKa = 11.84YY306 pKa = 9.78YY307 pKa = 10.75YY308 pKa = 9.65LQCGYY313 pKa = 10.63EE314 pKa = 3.88ITLDD318 pKa = 3.18EE319 pKa = 4.53CEE321 pKa = 4.44NYY323 pKa = 10.36EE324 pKa = 4.65EE325 pKa = 5.94AIHH328 pKa = 6.31CQTALIKK335 pKa = 9.74TPTPIMVRR343 pKa = 11.84KK344 pKa = 8.77PWDD347 pKa = 3.97TISKK351 pKa = 10.41CMVSQKK357 pKa = 10.62YY358 pKa = 7.56FTDD361 pKa = 3.3SKK363 pKa = 9.03TASAFLHH370 pKa = 6.04QMATCEE376 pKa = 4.02LSIHH380 pKa = 6.32RR381 pKa = 11.84GVPIMQSFFRR391 pKa = 11.84MILRR395 pKa = 11.84STKK398 pKa = 9.89PNNRR402 pKa = 11.84NYY404 pKa = 10.61SDD406 pKa = 3.96EE407 pKa = 4.2FQHH410 pKa = 7.2RR411 pKa = 11.84LVLVKK416 pKa = 8.99QLCDD420 pKa = 3.16NRR422 pKa = 11.84QDD424 pKa = 3.03ITTEE428 pKa = 3.56ARR430 pKa = 11.84ISFEE434 pKa = 4.4LSFKK438 pKa = 10.94VDD440 pKa = 2.72IATQYY445 pKa = 9.14YY446 pKa = 8.07WEE448 pKa = 4.34EE449 pKa = 4.12VFDD452 pKa = 3.69NWKK455 pKa = 10.33LDD457 pKa = 3.31TSIFDD462 pKa = 3.63SQHH465 pKa = 4.74

Molecular weight: 54.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

720

255

465

360.0

41.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.278 ± 1.054

2.5 ± 0.642

5.0 ± 0.333

5.833 ± 0.404

3.472 ± 0.162

3.611 ± 0.531

3.194 ± 0.028

5.694 ± 0.67

6.528 ± 0.884

9.167 ± 0.119

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.778 ± 0.016

4.028 ± 0.329

5.417 ± 0.796

5.972 ± 0.147

6.528 ± 0.503

6.944 ± 0.436

7.083 ± 0.392

4.722 ± 0.372

1.667 ± 0.238

4.583 ± 0.321
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski