Rhodococcus sp. OK302

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Rhodococcus; unclassified Rhodococcus

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6148 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A235GA69|A0A235GA69_9NOCA Uncharacterized protein (TIGR02680 family) OS=Rhodococcus sp. OK302 OX=1882769 GN=BDB13_4089 PE=4 SV=1
MM1 pKa = 7.18TNLYY5 pKa = 8.61RR6 pKa = 11.84TGRR9 pKa = 11.84GHH11 pKa = 6.06WRR13 pKa = 11.84SGLVAAILLVLAGCSASPSEE33 pKa = 4.59TEE35 pKa = 4.15SADD38 pKa = 3.44PAGPLPLGDD47 pKa = 3.55FTYY50 pKa = 10.88AANPCLDD57 pKa = 4.01PLIPGAPLPPFGDD70 pKa = 4.27DD71 pKa = 3.79FTCGTLIVPQNRR83 pKa = 11.84SQPGGATITIPVARR97 pKa = 11.84QRR99 pKa = 11.84AQNPQSEE106 pKa = 4.56MPPLLMLSGGPGGSGLLDD124 pKa = 3.43GLISYY129 pKa = 10.42SGLDD133 pKa = 3.54LNKK136 pKa = 10.45DD137 pKa = 3.0RR138 pKa = 11.84DD139 pKa = 4.0VIYY142 pKa = 10.16IDD144 pKa = 3.45QRR146 pKa = 11.84GTLRR150 pKa = 11.84ATPFVACPQVDD161 pKa = 3.25AVMQSLLDD169 pKa = 3.67KK170 pKa = 10.37PYY172 pKa = 10.37TDD174 pKa = 4.58PATEE178 pKa = 4.04AAGTAAVAACSEE190 pKa = 4.14QFRR193 pKa = 11.84AQGVDD198 pKa = 3.11LDD200 pKa = 4.26VFNSLEE206 pKa = 3.97NAADD210 pKa = 3.87LASLRR215 pKa = 11.84LALEE219 pKa = 3.9IPEE222 pKa = 3.91WDD224 pKa = 3.65VYY226 pKa = 10.2GVSYY230 pKa = 11.02GSDD233 pKa = 3.0LALQYY238 pKa = 11.19LRR240 pKa = 11.84DD241 pKa = 3.94FPDD244 pKa = 5.11GIRR247 pKa = 11.84AVVADD252 pKa = 4.17SVVPPQMNLADD263 pKa = 4.23TLWPNAARR271 pKa = 11.84GFDD274 pKa = 3.82ALEE277 pKa = 4.43AACDD281 pKa = 3.83AQPACQAMLPDD292 pKa = 4.02LTGTLATTVTDD303 pKa = 4.93LDD305 pKa = 3.47AHH307 pKa = 5.86PQVVSVTAGDD317 pKa = 4.21GSPVDD322 pKa = 3.94VMVDD326 pKa = 3.78GYY328 pKa = 11.72KK329 pKa = 10.37LASLVNSASLATNGLVDD346 pKa = 4.15MPAIIAAAGSGDD358 pKa = 3.82VTPAAQLLGSAQPSTVPLVGSGLTYY383 pKa = 10.84GVFCGEE389 pKa = 4.14AVAHH393 pKa = 6.23TSAEE397 pKa = 4.09AMFGAAKK404 pKa = 10.43NSLPQFPDD412 pKa = 2.97AVLSLTPQLPWLVGDD427 pKa = 4.31CAAWNVDD434 pKa = 3.53AVPDD438 pKa = 4.69LVVQPANSTVPVLLLSGGLDD458 pKa = 3.35GVTPPGNADD467 pKa = 3.01IAKK470 pKa = 8.2ATLPNSVALTFPEE483 pKa = 4.55SGHH486 pKa = 6.39GVLTQSDD493 pKa = 4.44CGPAAVTGFLDD504 pKa = 5.04DD505 pKa = 4.38PSASYY510 pKa = 10.63RR511 pKa = 11.84PQCLDD516 pKa = 3.35TVVIPPFSTSLVGSGG531 pKa = 3.6

Molecular weight: 54.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A235G705|A0A235G705_9NOCA Amino acid/polyamine/organocation transporter (APC superfamily) OS=Rhodococcus sp. OK302 OX=1882769 GN=BDB13_2916 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.72HH17 pKa = 5.63RR18 pKa = 11.84KK19 pKa = 7.56LLRR22 pKa = 11.84RR23 pKa = 11.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.11LGKK33 pKa = 9.87

Molecular weight: 4.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6148

0

6148

1987623

29

9545

323.3

34.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.061 ± 0.039

0.783 ± 0.01

6.095 ± 0.028

5.458 ± 0.027

3.165 ± 0.019

8.806 ± 0.033

2.076 ± 0.016

4.816 ± 0.022

2.478 ± 0.025

9.763 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.09 ± 0.015

2.509 ± 0.018

5.324 ± 0.022

2.883 ± 0.016

6.536 ± 0.035

6.474 ± 0.025

6.429 ± 0.039

8.765 ± 0.035

1.423 ± 0.011

2.067 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski