Amino acid dipepetide frequency for Rhodococcus sp. OK302

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	16.593AlaAla: 16.593 ± 0.132	0.887AlaCys: 0.887 ± 0.021	7.501AlaAsp: 7.501 ± 0.066	7.057AlaGlu: 7.057 ± 0.075	3.473AlaPhe: 3.473 ± 0.038	10.812AlaGly: 10.812 ± 0.097	2.396AlaHis: 2.396 ± 0.04	5.507AlaIle: 5.507 ± 0.062	3.175AlaLys: 3.175 ± 0.053	12.477AlaLeu: 12.477 ± 0.097	2.708AlaMet: 2.708 ± 0.041	2.564AlaAsn: 2.564 ± 0.038	5.734AlaPro: 5.734 ± 0.074	3.81AlaGln: 3.81 ± 0.05	7.312AlaArg: 7.312 ± 0.064	6.782AlaSer: 6.782 ± 0.053	7.031AlaThr: 7.031 ± 0.062	11.15AlaVal: 11.15 ± 0.088	1.436AlaTrp: 1.436 ± 0.032	2.202AlaTyr: 2.202 ± 0.039	0.0AlaXaa: 0.0 ± 0.0
Cys	1.005CysAla: 1.005 ± 0.025	0.1CysCys: 0.1 ± 0.007	0.51CysAsp: 0.51 ± 0.019	0.417CysGlu: 0.417 ± 0.018	0.213CysPhe: 0.213 ± 0.012	0.901CysGly: 0.901 ± 0.023	0.169CysHis: 0.169 ± 0.009	0.322CysIle: 0.322 ± 0.014	0.135CysLys: 0.135 ± 0.008	0.604CysLeu: 0.604 ± 0.017	0.119CysMet: 0.119 ± 0.009	0.183CysAsn: 0.183 ± 0.009	0.446CysPro: 0.446 ± 0.017	0.207CysGln: 0.207 ± 0.011	0.501CysArg: 0.501 ± 0.017	0.593CysSer: 0.593 ± 0.016	0.504CysThr: 0.504 ± 0.017	0.626CysVal: 0.626 ± 0.018	0.12CysTrp: 0.12 ± 0.008	0.16CysTyr: 0.16 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	7.227AspAla: 7.227 ± 0.063	0.476AspCys: 0.476 ± 0.015	3.492AspAsp: 3.492 ± 0.044	3.592AspGlu: 3.592 ± 0.05	1.824AspPhe: 1.824 ± 0.03	5.795AspGly: 5.795 ± 0.054	1.357AspHis: 1.357 ± 0.027	2.806AspIle: 2.806 ± 0.038	1.403AspLys: 1.403 ± 0.032	6.217AspLeu: 6.217 ± 0.066	1.055AspMet: 1.055 ± 0.023	1.487AspAsn: 1.487 ± 0.027	3.977AspPro: 3.977 ± 0.045	1.872AspGln: 1.872 ± 0.032	4.173AspArg: 4.173 ± 0.05	3.534AspSer: 3.534 ± 0.044	3.199AspThr: 3.199 ± 0.05	5.268AspVal: 5.268 ± 0.064	0.887AspTrp: 0.887 ± 0.023	1.308AspTyr: 1.308 ± 0.026	0.0AspXaa: 0.0 ± 0.0
Glu	5.648GluAla: 5.648 ± 0.068	0.389GluCys: 0.389 ± 0.015	2.593GluAsp: 2.593 ± 0.036	2.685GluGlu: 2.685 ± 0.05	2.021GluPhe: 2.021 ± 0.038	3.573GluGly: 3.573 ± 0.051	1.469GluHis: 1.469 ± 0.027	3.026GluIle: 3.026 ± 0.044	1.682GluLys: 1.682 ± 0.036	6.65GluLeu: 6.65 ± 0.08	1.136GluMet: 1.136 ± 0.028	1.595GluAsn: 1.595 ± 0.033	2.687GluPro: 2.687 ± 0.047	2.221GluGln: 2.221 ± 0.034	4.201GluArg: 4.201 ± 0.051	3.701GluSer: 3.701 ± 0.05	2.962GluThr: 2.962 ± 0.045	4.262GluVal: 4.262 ± 0.049	0.828GluTrp: 0.828 ± 0.025	1.248GluTyr: 1.248 ± 0.03	0.0GluXaa: 0.0 ± 0.0
Phe	3.934PheAla: 3.934 ± 0.048	0.312PheCys: 0.312 ± 0.014	2.589PheAsp: 2.589 ± 0.047	1.787PheGlu: 1.787 ± 0.035	1.021PhePhe: 1.021 ± 0.025	3.586PheGly: 3.586 ± 0.054	0.644PheHis: 0.644 ± 0.018	1.198PheIle: 1.198 ± 0.032	0.611PheLys: 0.611 ± 0.021	2.638PheLeu: 2.638 ± 0.036	0.529PheMet: 0.529 ± 0.017	0.793PheAsn: 0.793 ± 0.02	1.464PhePro: 1.464 ± 0.032	0.737PheGln: 0.737 ± 0.024	1.75PheArg: 1.75 ± 0.03	2.024PheSer: 2.024 ± 0.035	2.197PheThr: 2.197 ± 0.038	2.737PheVal: 2.737 ± 0.04	0.419PheTrp: 0.419 ± 0.017	0.677PheTyr: 0.677 ± 0.02	0.0PheXaa: 0.0 ± 0.0
Gly	9.457GlyAla: 9.457 ± 0.09	0.757GlyCys: 0.757 ± 0.021	4.723GlyAsp: 4.723 ± 0.047	4.471GlyGlu: 4.471 ± 0.056	3.204GlyPhe: 3.204 ± 0.044	7.7GlyGly: 7.7 ± 0.087	1.823GlyHis: 1.823 ± 0.032	4.848GlyIle: 4.848 ± 0.06	2.71GlyLys: 2.71 ± 0.043	8.497GlyLeu: 8.497 ± 0.082	2.067GlyMet: 2.067 ± 0.032	2.4GlyAsn: 2.4 ± 0.044	3.985GlyPro: 3.985 ± 0.049	2.652GlyGln: 2.652 ± 0.043	5.518GlyArg: 5.518 ± 0.056	6.186GlySer: 6.186 ± 0.064	5.832GlyThr: 5.832 ± 0.062	7.482GlyVal: 7.482 ± 0.071	1.492GlyTrp: 1.492 ± 0.03	2.254GlyTyr: 2.254 ± 0.04	0.0GlyXaa: 0.0 ± 0.0
His	2.164HisAla: 2.164 ± 0.039	0.227HisCys: 0.227 ± 0.011	1.226HisAsp: 1.226 ± 0.023	1.065HisGlu: 1.065 ± 0.026	0.696HisPhe: 0.696 ± 0.018	1.893HisGly: 1.893 ± 0.03	0.671HisHis: 0.671 ± 0.022	0.858HisIle: 0.858 ± 0.02	0.393HisLys: 0.393 ± 0.015	2.079HisLeu: 2.079 ± 0.035	0.364HisMet: 0.364 ± 0.014	0.497HisAsn: 0.497 ± 0.016	1.453HisPro: 1.453 ± 0.028	0.623HisGln: 0.623 ± 0.018	1.64HisArg: 1.64 ± 0.031	1.324HisSer: 1.324 ± 0.029	1.165HisThr: 1.165 ± 0.028	1.605HisVal: 1.605 ± 0.03	0.314HisTrp: 0.314 ± 0.013	0.502HisTyr: 0.502 ± 0.017	0.0HisXaa: 0.0 ± 0.0
Ile	6.935IleAla: 6.935 ± 0.071	0.41IleCys: 0.41 ± 0.015	3.651IleAsp: 3.651 ± 0.043	3.006IleGlu: 3.006 ± 0.042	1.312IlePhe: 1.312 ± 0.032	4.857IleGly: 4.857 ± 0.057	0.861IleHis: 0.861 ± 0.021	1.762IleIle: 1.762 ± 0.036	0.979IleLys: 0.979 ± 0.023	3.982IleLeu: 3.982 ± 0.05	0.742IleMet: 0.742 ± 0.022	1.191IleAsn: 1.191 ± 0.026	2.663IlePro: 2.663 ± 0.039	0.981IleGln: 0.981 ± 0.019	2.847IleArg: 2.847 ± 0.039	3.115IleSer: 3.115 ± 0.043	3.195IleThr: 3.195 ± 0.04	4.335IleVal: 4.335 ± 0.057	0.482IleTrp: 0.482 ± 0.014	0.85IleTyr: 0.85 ± 0.023	0.0IleXaa: 0.0 ± 0.0
Lys	2.972LysAla: 2.972 ± 0.054	0.136LysCys: 0.136 ± 0.009	1.299LysAsp: 1.299 ± 0.03	1.11LysGlu: 1.11 ± 0.03	0.845LysPhe: 0.845 ± 0.021	1.709LysGly: 1.709 ± 0.035	0.523LysHis: 0.523 ± 0.016	1.381LysIle: 1.381 ± 0.032	0.905LysLys: 0.905 ± 0.029	2.389LysLeu: 2.389 ± 0.039	0.602LysMet: 0.602 ± 0.018	0.733LysAsn: 0.733 ± 0.022	1.449LysPro: 1.449 ± 0.033	0.757LysGln: 0.757 ± 0.021	1.596LysArg: 1.596 ± 0.031	1.622LysSer: 1.622 ± 0.033	1.566LysThr: 1.566 ± 0.032	2.205LysVal: 2.205 ± 0.039	0.371LysTrp: 0.371 ± 0.014	0.617LysTyr: 0.617 ± 0.019	0.0LysXaa: 0.0 ± 0.0
Leu	12.857LeuAla: 12.857 ± 0.094	0.803LeuCys: 0.803 ± 0.02	6.665LeuAsp: 6.665 ± 0.073	5.09LeuGlu: 5.09 ± 0.056	2.799LeuPhe: 2.799 ± 0.043	9.017LeuGly: 9.017 ± 0.073	1.928LeuHis: 1.928 ± 0.034	4.649LeuIle: 4.649 ± 0.06	1.882LeuLys: 1.882 ± 0.04	9.136LeuLeu: 9.136 ± 0.091	1.85LeuMet: 1.85 ± 0.034	2.171LeuAsn: 2.171 ± 0.035	5.425LeuPro: 5.425 ± 0.061	2.34LeuGln: 2.34 ± 0.035	6.827LeuArg: 6.827 ± 0.061	6.449LeuSer: 6.449 ± 0.066	6.587LeuThr: 6.587 ± 0.061	8.387LeuVal: 8.387 ± 0.073	1.154LeuTrp: 1.154 ± 0.023	1.611LeuTyr: 1.611 ± 0.029	0.0LeuXaa: 0.0 ± 0.0
Met	2.326MetAla: 2.326 ± 0.04	0.181MetCys: 0.181 ± 0.009	0.96MetAsp: 0.96 ± 0.022	0.761MetGlu: 0.761 ± 0.022	0.694MetPhe: 0.694 ± 0.019	1.484MetGly: 1.484 ± 0.029	0.435MetHis: 0.435 ± 0.015	1.141MetIle: 1.141 ± 0.026	0.58MetLys: 0.58 ± 0.016	2.088MetLeu: 2.088 ± 0.035	0.476MetMet: 0.476 ± 0.017	0.637MetAsn: 0.637 ± 0.017	1.116MetPro: 1.116 ± 0.025	0.571MetGln: 0.571 ± 0.017	1.384MetArg: 1.384 ± 0.03	1.838MetSer: 1.838 ± 0.032	1.918MetThr: 1.918 ± 0.034	1.608MetVal: 1.608 ± 0.031	0.286MetTrp: 0.286 ± 0.013	0.416MetTyr: 0.416 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	2.854AsnAla: 2.854 ± 0.041	0.213AsnCys: 0.213 ± 0.011	1.252AsnAsp: 1.252 ± 0.024	1.166AsnGlu: 1.166 ± 0.024	0.791AsnPhe: 0.791 ± 0.025	2.313AsnGly: 2.313 ± 0.041	0.54AsnHis: 0.54 ± 0.017	1.164AsnIle: 1.164 ± 0.025	0.624AsnLys: 0.624 ± 0.019	2.386AsnLeu: 2.386 ± 0.041	0.515AsnMet: 0.515 ± 0.016	0.744AsnAsn: 0.744 ± 0.024	1.897AsnPro: 1.897 ± 0.035	0.726AsnGln: 0.726 ± 0.02	1.615AsnArg: 1.615 ± 0.03	1.687AsnSer: 1.687 ± 0.03	1.629AsnThr: 1.629 ± 0.032	1.969AsnVal: 1.969 ± 0.035	0.402AsnTrp: 0.402 ± 0.014	0.605AsnTyr: 0.605 ± 0.019	0.0AsnXaa: 0.0 ± 0.0
Pro	6.272ProAla: 6.272 ± 0.071	0.255ProCys: 0.255 ± 0.012	3.777ProAsp: 3.777 ± 0.049	3.685ProGlu: 3.685 ± 0.044	1.542ProPhe: 1.542 ± 0.025	4.856ProGly: 4.856 ± 0.063	1.037ProHis: 1.037 ± 0.024	2.46ProIle: 2.46 ± 0.034	1.402ProLys: 1.402 ± 0.028	4.644ProLeu: 4.644 ± 0.054	1.114ProMet: 1.114 ± 0.028	1.42ProAsn: 1.42 ± 0.03	2.475ProPro: 2.475 ± 0.058	1.552ProGln: 1.552 ± 0.033	2.955ProArg: 2.955 ± 0.042	3.443ProSer: 3.443 ± 0.04	3.694ProThr: 3.694 ± 0.056	4.804ProVal: 4.804 ± 0.067	0.766ProTrp: 0.766 ± 0.022	1.087ProTyr: 1.087 ± 0.026	0.0ProXaa: 0.0 ± 0.0
Gln	3.236GlnAla: 3.236 ± 0.042	0.231GlnCys: 0.231 ± 0.012	1.265GlnAsp: 1.265 ± 0.026	1.261GlnGlu: 1.261 ± 0.026	1.104GlnPhe: 1.104 ± 0.029	1.964GlnGly: 1.964 ± 0.037	0.642GlnHis: 0.642 ± 0.019	1.628GlnIle: 1.628 ± 0.028	0.749GlnLys: 0.749 ± 0.021	3.326GlnLeu: 3.326 ± 0.046	0.679GlnMet: 0.679 ± 0.02	0.735GlnAsn: 0.735 ± 0.019	1.548GlnPro: 1.548 ± 0.03	1.212GlnGln: 1.212 ± 0.031	2.18GlnArg: 2.18 ± 0.034	1.824GlnSer: 1.824 ± 0.031	1.459GlnThr: 1.459 ± 0.032	2.584GlnVal: 2.584 ± 0.042	0.547GlnTrp: 0.547 ± 0.017	0.662GlnTyr: 0.662 ± 0.019	0.0GlnXaa: 0.0 ± 0.0
Arg	6.957ArgAla: 6.957 ± 0.069	0.519ArgCys: 0.519 ± 0.018	3.672ArgAsp: 3.672 ± 0.044	3.89ArgGlu: 3.89 ± 0.049	2.178ArgPhe: 2.178 ± 0.032	4.807ArgGly: 4.807 ± 0.053	1.441ArgHis: 1.441 ± 0.029	3.543ArgIle: 3.543 ± 0.045	1.791ArgLys: 1.791 ± 0.033	6.23ArgLeu: 6.23 ± 0.069	1.654ArgMet: 1.654 ± 0.027	1.721ArgAsn: 1.721 ± 0.031	3.187ArgPro: 3.187 ± 0.043	1.934ArgGln: 1.934 ± 0.032	5.252ArgArg: 5.252 ± 0.07	4.366ArgSer: 4.366 ± 0.049	4.424ArgThr: 4.424 ± 0.055	5.099ArgVal: 5.099 ± 0.056	1.118ArgTrp: 1.118 ± 0.026	1.581ArgTyr: 1.581 ± 0.029	0.0ArgXaa: 0.0 ± 0.0
Ser	7.921SerAla: 7.921 ± 0.077	0.453SerCys: 0.453 ± 0.016	3.922SerAsp: 3.922 ± 0.046	3.492SerGlu: 3.492 ± 0.049	1.93SerPhe: 1.93 ± 0.031	6.621SerGly: 6.621 ± 0.068	1.137SerHis: 1.137 ± 0.03	3.17SerIle: 3.17 ± 0.043	1.564SerLys: 1.564 ± 0.03	5.709SerLeu: 5.709 ± 0.055	1.661SerMet: 1.661 ± 0.028	1.51SerAsn: 1.51 ± 0.029	3.28SerPro: 3.28 ± 0.043	1.731SerGln: 1.731 ± 0.033	3.917SerArg: 3.917 ± 0.046	4.243SerSer: 4.243 ± 0.051	4.374SerThr: 4.374 ± 0.053	5.838SerVal: 5.838 ± 0.064	0.98SerTrp: 0.98 ± 0.023	1.283SerTyr: 1.283 ± 0.027	0.0SerXaa: 0.0 ± 0.0
Thr	7.892ThrAla: 7.892 ± 0.082	0.457ThrCys: 0.457 ± 0.014	3.901ThrAsp: 3.901 ± 0.051	3.55ThrGlu: 3.55 ± 0.041	2.049ThrPhe: 2.049 ± 0.033	5.935ThrGly: 5.935 ± 0.06	1.219ThrHis: 1.219 ± 0.027	2.845ThrIle: 2.845 ± 0.041	1.425ThrLys: 1.425 ± 0.029	6.004ThrLeu: 6.004 ± 0.06	1.257ThrMet: 1.257 ± 0.024	1.411ThrAsn: 1.411 ± 0.031	3.93ThrPro: 3.93 ± 0.049	1.594ThrGln: 1.594 ± 0.029	3.589ThrArg: 3.589 ± 0.042	4.021ThrSer: 4.021 ± 0.047	4.598ThrThr: 4.598 ± 0.094	6.335ThrVal: 6.335 ± 0.069	0.876ThrTrp: 0.876 ± 0.019	1.401ThrTyr: 1.401 ± 0.033	0.0ThrXaa: 0.0 ± 0.0
Val	10.69ValAla: 10.69 ± 0.097	0.675ValCys: 0.675 ± 0.018	5.963ValAsp: 5.963 ± 0.059	4.795ValGlu: 4.795 ± 0.052	2.643ValPhe: 2.643 ± 0.039	7.212ValGly: 7.212 ± 0.075	1.775ValHis: 1.775 ± 0.029	4.325ValIle: 4.325 ± 0.051	1.918ValLys: 1.918 ± 0.032	8.858ValLeu: 8.858 ± 0.086	1.655ValMet: 1.655 ± 0.027	2.257ValAsn: 2.257 ± 0.035	4.694ValPro: 4.694 ± 0.057	2.131ValGln: 2.131 ± 0.036	5.515ValArg: 5.515 ± 0.053	5.57ValSer: 5.57 ± 0.064	5.809ValThr: 5.809 ± 0.07	8.621ValVal: 8.621 ± 0.091	1.052ValTrp: 1.052 ± 0.022	1.494ValTyr: 1.494 ± 0.028	0.0ValXaa: 0.0 ± 0.0
Trp	1.467TrpAla: 1.467 ± 0.027	0.159TrpCys: 0.159 ± 0.008	0.742TrpAsp: 0.742 ± 0.021	0.633TrpGlu: 0.633 ± 0.017	0.535TrpPhe: 0.535 ± 0.018	1.04TrpGly: 1.04 ± 0.026	0.307TrpHis: 0.307 ± 0.012	0.758TrpIle: 0.758 ± 0.023	0.42TrpLys: 0.42 ± 0.015	1.483TrpLeu: 1.483 ± 0.03	0.382TrpMet: 0.382 ± 0.016	0.505TrpAsn: 0.505 ± 0.016	0.66TrpPro: 0.66 ± 0.021	0.528TrpGln: 0.528 ± 0.015	0.985TrpArg: 0.985 ± 0.023	1.015TrpSer: 1.015 ± 0.025	0.948TrpThr: 0.948 ± 0.023	1.04TrpVal: 1.04 ± 0.018	0.33TrpTrp: 0.33 ± 0.016	0.29TrpTyr: 0.29 ± 0.012	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.202TyrAla: 2.202 ± 0.034	0.193TyrCys: 0.193 ± 0.01	1.252TyrAsp: 1.252 ± 0.026	1.068TyrGlu: 1.068 ± 0.023	0.771TyrPhe: 0.771 ± 0.023	1.98TyrGly: 1.98 ± 0.03	0.382TyrHis: 0.382 ± 0.015	0.767TyrIle: 0.767 ± 0.024	0.436TyrLys: 0.436 ± 0.017	2.232TyrLeu: 2.232 ± 0.036	0.335TyrMet: 0.335 ± 0.013	0.536TyrAsn: 0.536 ± 0.017	1.169TyrPro: 1.169 ± 0.025	0.656TyrGln: 0.656 ± 0.023	1.609TyrArg: 1.609 ± 0.034	1.398TyrSer: 1.398 ± 0.024	1.2TyrThr: 1.2 ± 0.029	1.697TyrVal: 1.697 ± 0.035	0.365TyrTrp: 0.365 ± 0.013	0.421TyrTyr: 0.421 ± 0.016	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6148 proteins (1987623 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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