Tortoise microvirus 38
Average proteome isoelectric point is 7.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W6X2|A0A4P8W6X2_9VIRU Peptidase_M15_4 domain-containing protein OS=Tortoise microvirus 38 OX=2583141 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 10.06 FDD4 pKa = 4.63 PFSQQEE10 pKa = 3.97 VSTNEE15 pKa = 4.05 TVWFGKK21 pKa = 10.31 GRR23 pKa = 11.84 LRR25 pKa = 11.84 LSLSARR31 pKa = 11.84 CPLYY35 pKa = 10.5 IEE37 pKa = 4.5 AQGIEE42 pKa = 4.24 ALAGIAEE49 pKa = 4.4 TFDD52 pKa = 4.46 LDD54 pKa = 3.84 LSEE57 pKa = 4.48 EE58 pKa = 4.04 VSFRR62 pKa = 11.84 VEE64 pKa = 3.49 APEE67 pKa = 4.02 GVRR70 pKa = 11.84 AFLRR74 pKa = 11.84 VPEE77 pKa = 4.18 PTSFEE82 pKa = 4.11 PEE84 pKa = 3.9 GEE86 pKa = 4.14 VFTNIDD92 pKa = 3.75 RR93 pKa = 11.84 MPHH96 pKa = 5.68 EE97 pKa = 5.04 SGSVAEE103 pKa = 4.57 VTRR106 pKa = 11.84 AMRR109 pKa = 11.84 LFEE112 pKa = 4.34 LEE114 pKa = 3.24 RR115 pKa = 11.84 RR116 pKa = 11.84 AALRR120 pKa = 11.84 EE121 pKa = 3.78 IRR123 pKa = 11.84 EE124 pKa = 4.05 EE125 pKa = 4.08 AEE127 pKa = 3.8 EE128 pKa = 4.69 LRR130 pKa = 11.84 TLRR133 pKa = 11.84 QAAKK137 pKa = 9.42 PQAQPIHH144 pKa = 6.38 QGEE147 pKa = 4.75 GEE149 pKa = 4.13 AEE151 pKa = 4.09 ALVDD155 pKa = 4.11 EE156 pKa = 5.45 ADD158 pKa = 4.57 DD159 pKa = 4.2 GAAAA163 pKa = 4.39
Molecular weight: 18.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.729
IPC2_protein 4.596
IPC_protein 4.495
Toseland 4.368
ProMoST 4.571
Dawson 4.406
Bjellqvist 4.558
Wikipedia 4.228
Rodwell 4.329
Grimsley 4.279
Solomon 4.406
Lehninger 4.355
Nozaki 4.52
DTASelect 4.584
Thurlkill 4.342
EMBOSS 4.253
Sillero 4.596
Patrickios 4.202
IPC_peptide 4.418
IPC2_peptide 4.596
IPC2.peptide.svr19 4.524
Protein with the highest isoelectric point:
>tr|A0A4P8W692|A0A4P8W692_9VIRU Uncharacterized protein OS=Tortoise microvirus 38 OX=2583141 PE=4 SV=1
MM1 pKa = 6.95 VPNYY5 pKa = 10.48 RR6 pKa = 11.84 LFEE9 pKa = 4.3 KK10 pKa = 10.55 RR11 pKa = 11.84 SLPVVSQPVTEE22 pKa = 4.43 GRR24 pKa = 11.84 STVLNSTKK32 pKa = 10.66 AVLLHH37 pKa = 5.15 TAIRR41 pKa = 11.84 TILPALMGAVGALVATLAPIYY62 pKa = 10.67 HH63 pKa = 6.48 NAFCGITPP71 pKa = 4.38
Molecular weight: 7.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.264
IPC2_protein 9.531
IPC_protein 10.014
Toseland 10.028
ProMoST 9.838
Dawson 10.277
Bjellqvist 10.014
Wikipedia 10.482
Rodwell 10.496
Grimsley 10.365
Solomon 10.365
Lehninger 10.321
Nozaki 10.087
DTASelect 9.999
Thurlkill 10.116
EMBOSS 10.452
Sillero 10.204
Patrickios 10.423
IPC_peptide 10.35
IPC2_peptide 9.107
IPC2.peptide.svr19 8.34
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
1726
71
561
246.6
27.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.429 ± 0.666
0.753 ± 0.336
5.852 ± 0.629
6.373 ± 1.356
3.708 ± 0.311
7.648 ± 1.819
2.317 ± 0.755
3.882 ± 0.215
4.809 ± 0.708
8.691 ± 0.315
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.144 ± 0.117
3.94 ± 0.842
6.489 ± 0.436
3.592 ± 0.581
7.822 ± 1.035
5.156 ± 0.484
5.562 ± 0.537
6.779 ± 0.713
1.854 ± 0.473
2.202 ± 0.398
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here