Durio zibethinus (Durian)
Average proteome isoelectric point is 6.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 53026 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6P5XK65|A0A6P5XK65_DURZI benzyl alcohol O-benzoyltransferase-like OS=Durio zibethinus OX=66656 GN=LOC111284102 PE=3 SV=1
MM1 pKa = 7.88 PKK3 pKa = 10.39 FNFPTILILAILLLACEE20 pKa = 4.48 LAIISPVLAYY30 pKa = 10.88 NDD32 pKa = 3.39 VDD34 pKa = 4.01 IGDD37 pKa = 4.27 ISPSAVTDD45 pKa = 3.57 DD46 pKa = 5.56 DD47 pKa = 4.92 MITSSSLDD55 pKa = 3.31 AGQLEE60 pKa = 4.51 DD61 pKa = 4.52 LYY63 pKa = 10.89 QRR65 pKa = 11.84 CVVMLGDD72 pKa = 3.6 EE73 pKa = 4.62 CGDD76 pKa = 3.97 EE77 pKa = 4.08 IFDD80 pKa = 5.49 LIFRR84 pKa = 11.84 NDD86 pKa = 3.32 SLIVTDD92 pKa = 4.7 KK93 pKa = 11.35 DD94 pKa = 2.86 IGLVSKK100 pKa = 8.89 YY101 pKa = 10.88 CCDD104 pKa = 3.21 KK105 pKa = 10.64 LLNMGRR111 pKa = 11.84 NCHH114 pKa = 6.01 DD115 pKa = 3.19 QLVNVIAQTTHH126 pKa = 6.53 FSTNSSATVPRR137 pKa = 11.84 SSQVWDD143 pKa = 3.69 LCSLSSSS150 pKa = 3.53
Molecular weight: 16.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.736
IPC2_protein 3.923
IPC_protein 3.923
Toseland 3.694
ProMoST 4.113
Dawson 3.948
Bjellqvist 4.101
Wikipedia 3.923
Rodwell 3.757
Grimsley 3.605
Solomon 3.935
Lehninger 3.884
Nozaki 4.062
DTASelect 4.368
Thurlkill 3.77
EMBOSS 3.923
Sillero 4.062
Patrickios 1.036
IPC_peptide 3.923
IPC2_peptide 4.024
IPC2.peptide.svr19 3.919
Protein with the highest isoelectric point:
>tr|A0A6P5ZWJ5|A0A6P5ZWJ5_DURZI Peptidyl-prolyl cis-trans isomerase OS=Durio zibethinus OX=66656 GN=LOC111304638 PE=3 SV=1
MM1 pKa = 6.7 YY2 pKa = 10.1 HH3 pKa = 6.64 SLRR6 pKa = 11.84 SQSLSPRR13 pKa = 11.84 SCFPARR19 pKa = 11.84 RR20 pKa = 11.84 RR21 pKa = 11.84 PSYY24 pKa = 10.1 SRR26 pKa = 11.84 QRR28 pKa = 11.84 SRR30 pKa = 11.84 SHH32 pKa = 5.84 SLSRR36 pKa = 11.84 SPSPVRR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 LRR46 pKa = 11.84 SPYY49 pKa = 9.74 QRR51 pKa = 11.84 WSPTLVRR58 pKa = 11.84 RR59 pKa = 11.84 RR60 pKa = 11.84 YY61 pKa = 9.42 LSPICRR67 pKa = 11.84 HH68 pKa = 6.2 RR69 pKa = 11.84 SPLSIRR75 pKa = 11.84 RR76 pKa = 11.84 RR77 pKa = 11.84 RR78 pKa = 11.84 SSSLVRR84 pKa = 11.84 RR85 pKa = 11.84 HH86 pKa = 6.21 RR87 pKa = 11.84 SPSTVFRR94 pKa = 11.84 RR95 pKa = 11.84 SSSPVRR101 pKa = 11.84 RR102 pKa = 11.84 RR103 pKa = 11.84 SASSVCRR110 pKa = 11.84 RR111 pKa = 11.84 SPLPMCRR118 pKa = 11.84 RR119 pKa = 11.84 SPSPLRR125 pKa = 11.84 HH126 pKa = 6.35 RR127 pKa = 11.84 SPHH130 pKa = 4.11 MQRR133 pKa = 11.84 RR134 pKa = 11.84 SPSSVRR140 pKa = 11.84 QRR142 pKa = 11.84 HH143 pKa = 5.51 HH144 pKa = 6.95 RR145 pKa = 11.84 LSSTPRR151 pKa = 11.84 HH152 pKa = 5.86 RR153 pKa = 11.84 SSSLVRR159 pKa = 11.84 RR160 pKa = 11.84 RR161 pKa = 11.84 SPGSNRR167 pKa = 11.84 RR168 pKa = 11.84 SITPSHH174 pKa = 6.23 GKK176 pKa = 9.56 SLSLYY181 pKa = 8.9 QSCSLSPVQHH191 pKa = 6.7 RR192 pKa = 11.84 SSSPLKK198 pKa = 10.41 RR199 pKa = 11.84 SPKK202 pKa = 10.17 DD203 pKa = 3.09
Molecular weight: 23.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.353
IPC2_protein 10.95
IPC_protein 12.354
Toseland 12.515
ProMoST 13.013
Dawson 12.515
Bjellqvist 12.515
Wikipedia 12.998
Rodwell 12.047
Grimsley 12.559
Solomon 13.013
Lehninger 12.91
Nozaki 12.515
DTASelect 12.515
Thurlkill 12.515
EMBOSS 13.013
Sillero 12.515
Patrickios 11.754
IPC_peptide 13.013
IPC2_peptide 12.003
IPC2.peptide.svr19 9.153
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
35616
17410
53026
26388952
31
5460
497.7
55.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.651 ± 0.008
1.882 ± 0.005
5.28 ± 0.007
6.555 ± 0.012
4.217 ± 0.006
6.406 ± 0.01
2.371 ± 0.004
5.349 ± 0.008
6.135 ± 0.01
9.934 ± 0.015
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.382 ± 0.003
4.574 ± 0.007
4.835 ± 0.009
3.881 ± 0.008
5.209 ± 0.008
9.231 ± 0.012
4.736 ± 0.006
6.386 ± 0.007
1.245 ± 0.003
2.738 ± 0.005
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here