Xanthomonas virus CP1
Average proteome isoelectric point is 6.65
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 47 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I7GY99|I7GY99_9CAUD GIY-YIG domain-containing protein OS=Xanthomonas virus CP1 OX=1188794 PE=4 SV=1
MM1 pKa = 7.58 YY2 pKa = 10.24 YY3 pKa = 10.09 RR4 pKa = 11.84 VDD6 pKa = 3.91 IEE8 pKa = 4.66 FEE10 pKa = 5.02 DD11 pKa = 3.3 IHH13 pKa = 8.29 GPEE16 pKa = 4.48 SLDD19 pKa = 3.55 EE20 pKa = 4.49 DD21 pKa = 3.9 TLEE24 pKa = 4.34 YY25 pKa = 11.15 YY26 pKa = 9.43 LAKK29 pKa = 10.5 YY30 pKa = 8.4 IRR32 pKa = 11.84 KK33 pKa = 9.0 YY34 pKa = 10.46 CGQAYY39 pKa = 8.12 PVEE42 pKa = 4.37 VVVMDD47 pKa = 3.83 KK48 pKa = 11.3 DD49 pKa = 4.33 EE50 pKa = 4.17 YY51 pKa = 11.04 CITYY55 pKa = 9.62 QLQVQWHH62 pKa = 7.42 RR63 pKa = 11.84 DD64 pKa = 3.43 TKK66 pKa = 11.14 DD67 pKa = 2.67 WLGPYY72 pKa = 10.09 GMQDD76 pKa = 2.99 VVGKK80 pKa = 8.1 VLRR83 pKa = 11.84 DD84 pKa = 3.3 AGEE87 pKa = 4.14 YY88 pKa = 9.5 PVALSVEE95 pKa = 4.02 LLEE98 pKa = 5.02 FDD100 pKa = 4.53 EE101 pKa = 5.82 EE102 pKa = 4.21 EE103 pKa = 4.36 DD104 pKa = 3.54
Molecular weight: 12.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.905
IPC2_protein 4.101
IPC_protein 4.037
Toseland 3.846
ProMoST 4.113
Dawson 4.012
Bjellqvist 4.202
Wikipedia 3.91
Rodwell 3.872
Grimsley 3.77
Solomon 3.999
Lehninger 3.948
Nozaki 4.126
DTASelect 4.304
Thurlkill 3.884
EMBOSS 3.923
Sillero 4.151
Patrickios 0.604
IPC_peptide 3.999
IPC2_peptide 4.139
IPC2.peptide.svr19 4.079
Protein with the highest isoelectric point:
>tr|I7HDH9|I7HDH9_9CAUD Putative head protein OS=Xanthomonas virus CP1 OX=1188794 PE=4 SV=1
MM1 pKa = 7.53 RR2 pKa = 11.84 RR3 pKa = 11.84 GVTGIYY9 pKa = 10.27 AITSPSGRR17 pKa = 11.84 RR18 pKa = 11.84 YY19 pKa = 9.87 VGQAVCIRR27 pKa = 11.84 ARR29 pKa = 11.84 WRR31 pKa = 11.84 KK32 pKa = 9.03 HH33 pKa = 5.16 LSNLHH38 pKa = 6.69 RR39 pKa = 11.84 DD40 pKa = 3.45 QHH42 pKa = 6.43 HH43 pKa = 6.63 NPLLTNAFRR52 pKa = 11.84 KK53 pKa = 10.63 YY54 pKa = 10.55 GDD56 pKa = 3.63 ALVFSVIEE64 pKa = 3.92 EE65 pKa = 4.29 CLAEE69 pKa = 4.62 DD70 pKa = 3.9 LTARR74 pKa = 11.84 EE75 pKa = 4.31 QFHH78 pKa = 6.86 MDD80 pKa = 3.13 QTPPSMLLNVCPAAGSPLGMKK101 pKa = 10.34 RR102 pKa = 11.84 SPEE105 pKa = 3.46 TRR107 pKa = 11.84 ARR109 pKa = 11.84 MSAATKK115 pKa = 10.41 GKK117 pKa = 10.41 AKK119 pKa = 9.5 STEE122 pKa = 3.85 HH123 pKa = 6.43 RR124 pKa = 11.84 AKK126 pKa = 10.57 LAAANKK132 pKa = 9.83 GKK134 pKa = 8.03 TLPAEE139 pKa = 4.04 QCAKK143 pKa = 10.07 IAEE146 pKa = 4.25 ALRR149 pKa = 11.84 GRR151 pKa = 11.84 VHH153 pKa = 6.44 SAEE156 pKa = 4.13 ARR158 pKa = 11.84 TKK160 pKa = 9.39 QAEE163 pKa = 4.11 AVRR166 pKa = 11.84 GKK168 pKa = 9.18 MLPNNTSGFCGVRR181 pKa = 11.84 RR182 pKa = 11.84 NGTGWQAKK190 pKa = 9.78 LGLLGSSVYY199 pKa = 10.41 FPTRR203 pKa = 11.84 PSPEE207 pKa = 3.92 AAWALRR213 pKa = 11.84 EE214 pKa = 3.91 NFLRR218 pKa = 11.84 ILYY221 pKa = 10.25 YY222 pKa = 10.25 YY223 pKa = 10.45 DD224 pKa = 3.54 HH225 pKa = 7.03 SS226 pKa = 4.0
Molecular weight: 25.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.323
IPC2_protein 9.575
IPC_protein 10.043
Toseland 10.423
ProMoST 10.087
Dawson 10.57
Bjellqvist 10.262
Wikipedia 10.745
Rodwell 10.877
Grimsley 10.628
Solomon 10.628
Lehninger 10.599
Nozaki 10.438
DTASelect 10.248
Thurlkill 10.438
EMBOSS 10.818
Sillero 10.496
Patrickios 10.57
IPC_peptide 10.628
IPC2_peptide 9.268
IPC2.peptide.svr19 8.554
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
47
0
47
12615
66
1573
268.4
29.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.702 ± 0.691
1.118 ± 0.192
5.295 ± 0.232
5.612 ± 0.284
2.933 ± 0.188
8.514 ± 0.279
2.013 ± 0.307
4.233 ± 0.202
4.962 ± 0.369
8.617 ± 0.261
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.418 ± 0.208
4.027 ± 0.232
3.813 ± 0.27
4.511 ± 0.396
5.937 ± 0.297
6.92 ± 0.351
6.294 ± 0.425
7.071 ± 0.282
1.736 ± 0.144
3.274 ± 0.251
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here