Vibrio ponticus
Average proteome isoelectric point is 6.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5653 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A090P6X1|A0A090P6X1_9VIBR Dihydrodipicolinate synthase family OS=Vibrio ponticus OX=265668 GN=JCM19238_1985 PE=4 SV=1
MM1 pKa = 7.95 DD2 pKa = 4.37 ARR4 pKa = 11.84 VGYY7 pKa = 9.95 RR8 pKa = 11.84 VADD11 pKa = 3.87 FDD13 pKa = 5.4 LSAFYY18 pKa = 11.35 SNTDD22 pKa = 2.6 VDD24 pKa = 4.23 KK25 pKa = 10.94 DD26 pKa = 3.72 YY27 pKa = 11.49 QAAQTNPVDD36 pKa = 3.54 DD37 pKa = 4.09 HH38 pKa = 7.9 SGYY41 pKa = 10.35 GVEE44 pKa = 4.28 VRR46 pKa = 11.84 FAGIEE51 pKa = 4.06 NVNLAAAYY59 pKa = 8.64 YY60 pKa = 7.79 ATSSDD65 pKa = 3.96 TDD67 pKa = 3.44 NDD69 pKa = 3.98 DD70 pKa = 4.0 NNVVAFAADD79 pKa = 3.44 YY80 pKa = 11.52 TMDD83 pKa = 2.86 KK84 pKa = 8.84 WTFATGYY91 pKa = 10.5 SIGDD95 pKa = 3.64 HH96 pKa = 7.18 DD97 pKa = 6.06 NNAKK101 pKa = 10.61 DD102 pKa = 3.5 QDD104 pKa = 3.73 NWFLNAGYY112 pKa = 10.37 GIAPNTTVYY121 pKa = 11.15 AEE123 pKa = 4.05 VGGEE127 pKa = 4.03 DD128 pKa = 3.94 KK129 pKa = 11.25 EE130 pKa = 5.29 GVDD133 pKa = 3.95 TDD135 pKa = 3.55 TALAIGVKK143 pKa = 10.5 AEE145 pKa = 3.97 FF146 pKa = 4.16
Molecular weight: 15.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.682
IPC2_protein 3.732
IPC_protein 3.745
Toseland 3.516
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.923
Wikipedia 3.732
Rodwell 3.567
Grimsley 3.427
Solomon 3.745
Lehninger 3.706
Nozaki 3.884
DTASelect 4.177
Thurlkill 3.579
EMBOSS 3.745
Sillero 3.872
Patrickios 1.036
IPC_peptide 3.745
IPC2_peptide 3.846
IPC2.peptide.svr19 3.786
Protein with the highest isoelectric point:
>tr|A0A090PDT3|A0A090PDT3_9VIBR Lipoprotein releasing system transmembrane protein LolE OS=Vibrio ponticus OX=265668 GN=JCM19238_5457 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.5 RR3 pKa = 11.84 TFQPTVLKK11 pKa = 10.46 RR12 pKa = 11.84 KK13 pKa = 7.65 RR14 pKa = 11.84 THH16 pKa = 5.89 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.44 NGRR28 pKa = 11.84 KK29 pKa = 9.39 VINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.22 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LSKK44 pKa = 10.83
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.705
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.427
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5653
0
5653
1295991
37
2466
229.3
25.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.957 ± 0.038
1.077 ± 0.014
5.424 ± 0.037
6.32 ± 0.036
4.089 ± 0.025
6.688 ± 0.036
2.22 ± 0.021
6.314 ± 0.026
5.089 ± 0.028
10.473 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.81 ± 0.021
4.17 ± 0.024
3.787 ± 0.023
4.871 ± 0.035
4.497 ± 0.03
6.54 ± 0.03
5.299 ± 0.028
7.157 ± 0.029
1.233 ± 0.012
2.985 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here