Kytococcus aerolatus
Average proteome isoelectric point is 5.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2173 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A212U5C1|A0A212U5C1_9MICO Sulfate permease SulP family OS=Kytococcus aerolatus OX=592308 GN=SAMN05445756_1944 PE=4 SV=1
MM1 pKa = 7.62 ARR3 pKa = 11.84 LEE5 pKa = 4.03 QAGITVTKK13 pKa = 10.52 AAGNDD18 pKa = 3.47 AEE20 pKa = 4.62 DD21 pKa = 3.72 ACGFMPGNSPGGITVGATGSYY42 pKa = 9.98 AGEE45 pKa = 3.84 VDD47 pKa = 3.72 VQADD51 pKa = 3.55 YY52 pKa = 11.84 SNFGSCVDD60 pKa = 3.51 IMAPGTGINSASNADD75 pKa = 4.06 DD76 pKa = 4.09 SSLLLMDD83 pKa = 4.86 GTSMSTPHH91 pKa = 5.12 VTGAAALYY99 pKa = 10.42 LEE101 pKa = 4.5 QHH103 pKa = 7.0 PDD105 pKa = 3.17 AAPDD109 pKa = 3.49 EE110 pKa = 4.83 VYY112 pKa = 10.47 QALLDD117 pKa = 3.91 AAAVDD122 pKa = 4.35 KK123 pKa = 11.35 LSGLNGSPNLLLNIEE138 pKa = 4.28 NLIEE142 pKa = 4.31 EE143 pKa = 5.11 PEE145 pKa = 4.26 PEE147 pKa = 4.19 PTPEE151 pKa = 4.86 PGLEE155 pKa = 4.02 RR156 pKa = 11.84 WWGADD161 pKa = 3.09 RR162 pKa = 11.84 YY163 pKa = 8.97 GTAAEE168 pKa = 4.21 TAVKK172 pKa = 10.01 SGQADD177 pKa = 3.6 TVYY180 pKa = 10.17 IASGQGFADD189 pKa = 3.59 AVTGGTAAQDD199 pKa = 3.68 VPVLLTQPDD208 pKa = 3.82 QASSPPAWTTPTPSPAAPPPRR229 pKa = 11.84 PRR231 pKa = 11.84 TAPCSS236 pKa = 3.57
Molecular weight: 23.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.738
IPC2_protein 3.795
IPC_protein 3.783
Toseland 3.567
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.694
Rodwell 3.605
Grimsley 3.478
Solomon 3.757
Lehninger 3.719
Nozaki 3.884
DTASelect 4.101
Thurlkill 3.617
EMBOSS 3.706
Sillero 3.897
Patrickios 1.875
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.823
Protein with the highest isoelectric point:
>tr|A0A212THL4|A0A212THL4_9MICO Osmoprotectant transport system ATP-binding protein OS=Kytococcus aerolatus OX=592308 GN=SAMN05445756_1356 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 ARR15 pKa = 11.84 KK16 pKa = 8.24 HH17 pKa = 4.6 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MSTRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILAARR35 pKa = 11.84 RR36 pKa = 11.84 GKK38 pKa = 10.47 GRR40 pKa = 11.84 SKK42 pKa = 11.09 LSAA45 pKa = 3.74
Molecular weight: 5.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2173
0
2173
726268
29
1652
334.2
35.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.095 ± 0.066
0.692 ± 0.014
5.827 ± 0.045
6.914 ± 0.056
2.499 ± 0.03
9.566 ± 0.044
2.362 ± 0.023
3.129 ± 0.036
1.784 ± 0.033
10.341 ± 0.069
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.01 ± 0.022
1.582 ± 0.023
5.887 ± 0.041
3.077 ± 0.031
7.732 ± 0.063
4.988 ± 0.035
6.065 ± 0.039
9.203 ± 0.046
1.614 ± 0.025
1.631 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here