Beihai rhabdo-like virus 6
Average proteome isoelectric point is 7.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KMN0|A0A1L3KMN0_9MONO Putative glycoprotein OS=Beihai rhabdo-like virus 6 OX=1922656 PE=4 SV=1
MM1 pKa = 6.85 TAAGPFTKK9 pKa = 10.13 RR10 pKa = 11.84 PRR12 pKa = 11.84 HH13 pKa = 5.43 YY14 pKa = 10.15 EE15 pKa = 3.6 SDD17 pKa = 3.41 MVSLKK22 pKa = 10.36 RR23 pKa = 11.84 HH24 pKa = 5.77 APSFTPLQMPHH35 pKa = 6.92 EE36 pKa = 4.37 IDD38 pKa = 3.15 IRR40 pKa = 11.84 SRR42 pKa = 11.84 LYY44 pKa = 11.04 NGTTTAIDD52 pKa = 3.18 WVAAVAYY59 pKa = 8.77 QYY61 pKa = 11.09 RR62 pKa = 11.84 LSSEE66 pKa = 4.42 VIAKK70 pKa = 9.58 LFSPYY75 pKa = 11.13 ALDD78 pKa = 3.26 QHH80 pKa = 6.34 NRR82 pKa = 11.84 PLYY85 pKa = 9.9 FPRR88 pKa = 11.84 FDD90 pKa = 3.73 TEE92 pKa = 3.86 DD93 pKa = 3.25 AKK95 pKa = 10.83 IANIRR100 pKa = 11.84 ARR102 pKa = 11.84 VKK104 pKa = 10.93 DD105 pKa = 3.5 SGKK108 pKa = 10.64 EE109 pKa = 3.74 GLEE112 pKa = 3.79 AARR115 pKa = 11.84 VGIQIAAYY123 pKa = 8.64 AVIVGLHH130 pKa = 5.25 KK131 pKa = 9.74 TIEE134 pKa = 4.53 DD135 pKa = 3.6 KK136 pKa = 11.32 NKK138 pKa = 9.74 EE139 pKa = 4.04 YY140 pKa = 10.81 VLRR143 pKa = 11.84 RR144 pKa = 11.84 IAAVGASQDD153 pKa = 4.76 DD154 pKa = 4.45 EE155 pKa = 4.51 ILTNIPVADD164 pKa = 3.39 MDD166 pKa = 3.77 YY167 pKa = 10.02 WVVVDD172 pKa = 6.16 RR173 pKa = 11.84 INDD176 pKa = 3.13 AHH178 pKa = 7.33 KK179 pKa = 10.22 YY180 pKa = 8.89 IRR182 pKa = 11.84 AAEE185 pKa = 3.95 ITNDD189 pKa = 4.0 LLHH192 pKa = 6.95 DD193 pKa = 3.85 VCTSVDD199 pKa = 3.74 DD200 pKa = 5.23 DD201 pKa = 4.08 STPLHH206 pKa = 6.54 IAMVDD211 pKa = 3.74 SIKK214 pKa = 10.3 MVLKK218 pKa = 10.47 GYY220 pKa = 10.97 GMLGTQVMANFLTTPSKK237 pKa = 10.63 AWLMAQVLDD246 pKa = 3.9 EE247 pKa = 5.08 GIAFKK252 pKa = 10.71 RR253 pKa = 11.84 ALRR256 pKa = 11.84 TFKK259 pKa = 10.76 ALHH262 pKa = 6.0 GDD264 pKa = 2.81 SWEE267 pKa = 3.87 WGRR270 pKa = 11.84 VLKK273 pKa = 11.08 LNTIEE278 pKa = 4.39 TLDD281 pKa = 3.6 KK282 pKa = 10.48 TSYY285 pKa = 9.59 PHH287 pKa = 7.24 LYY289 pKa = 7.91 WAAAVRR295 pKa = 11.84 RR296 pKa = 11.84 NGGTGGQADD305 pKa = 4.04 GLKK308 pKa = 10.05 NYY310 pKa = 8.97 VMKK313 pKa = 10.9 APDD316 pKa = 3.46 GLMVNKK322 pKa = 8.77 TQLEE326 pKa = 4.22 SWALKK331 pKa = 10.4 DD332 pKa = 3.55 LASAAVLTATFAQKK346 pKa = 10.14 IKK348 pKa = 9.41 EE349 pKa = 4.28 TLGINLDD356 pKa = 3.95 DD357 pKa = 4.05 KK358 pKa = 11.77 VGDD361 pKa = 3.84 KK362 pKa = 11.42 VEE364 pKa = 4.23 DD365 pKa = 3.65 LL366 pKa = 4.17
Molecular weight: 40.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.493
IPC2_protein 6.351
IPC_protein 6.576
Toseland 7.044
ProMoST 6.927
Dawson 6.927
Bjellqvist 6.81
Wikipedia 6.956
Rodwell 6.927
Grimsley 7.205
Solomon 6.956
Lehninger 6.956
Nozaki 7.117
DTASelect 7.337
Thurlkill 7.366
EMBOSS 7.38
Sillero 7.293
Patrickios 4.482
IPC_peptide 6.971
IPC2_peptide 6.927
IPC2.peptide.svr19 6.986
Protein with the highest isoelectric point:
>tr|A0A1L3KMM4|A0A1L3KMM4_9MONO Putative nucleoprotein OS=Beihai rhabdo-like virus 6 OX=1922656 PE=4 SV=1
MM1 pKa = 7.58 AEE3 pKa = 3.66 KK4 pKa = 10.42 SRR6 pKa = 11.84 RR7 pKa = 11.84 KK8 pKa = 9.79 KK9 pKa = 10.83 SLGEE13 pKa = 3.88 RR14 pKa = 11.84 TPGSISARR22 pKa = 11.84 VGDD25 pKa = 3.71 RR26 pKa = 11.84 AKK28 pKa = 10.51 KK29 pKa = 9.83 RR30 pKa = 11.84 KK31 pKa = 8.3 LTEE34 pKa = 4.13 EE35 pKa = 4.2 EE36 pKa = 4.63 DD37 pKa = 3.85 PLPAAIASFTEE48 pKa = 3.97 LMEE51 pKa = 4.61 GKK53 pKa = 9.74 FARR56 pKa = 11.84 VMEE59 pKa = 4.26 ILEE62 pKa = 4.61 EE63 pKa = 4.13 IRR65 pKa = 11.84 SQTAGMEE72 pKa = 4.0 ARR74 pKa = 11.84 ISSLEE79 pKa = 3.85 RR80 pKa = 11.84 RR81 pKa = 11.84 VGNMNVDD88 pKa = 3.44 PRR90 pKa = 11.84 GTTVTAPPVATIGRR104 pKa = 11.84 GRR106 pKa = 11.84 GRR108 pKa = 11.84 GLTLVPPRR116 pKa = 11.84 QIGSTAAVGRR126 pKa = 11.84 GRR128 pKa = 11.84 GGRR131 pKa = 11.84 GQPIAGPSSFRR142 pKa = 11.84 PRR144 pKa = 11.84 SPEE147 pKa = 3.68 TGSDD151 pKa = 3.65 DD152 pKa = 4.54 EE153 pKa = 4.81 EE154 pKa = 6.44 DD155 pKa = 3.8 GSAMDD160 pKa = 4.73 GQPSDD165 pKa = 5.16 DD166 pKa = 4.03 RR167 pKa = 11.84 YY168 pKa = 11.34 EE169 pKa = 4.41 DD170 pKa = 4.01 ADD172 pKa = 4.66 LLGLLL177 pKa = 4.64
Molecular weight: 18.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.548
IPC2_protein 8.317
IPC_protein 8.58
Toseland 9.472
ProMoST 8.975
Dawson 9.575
Bjellqvist 9.151
Wikipedia 9.677
Rodwell 9.853
Grimsley 9.589
Solomon 9.75
Lehninger 9.75
Nozaki 9.37
DTASelect 9.165
Thurlkill 9.443
EMBOSS 9.823
Sillero 9.487
Patrickios 9.736
IPC_peptide 9.75
IPC2_peptide 7.658
IPC2.peptide.svr19 7.501
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
2884
177
1832
721.0
80.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.079 ± 1.04
1.872 ± 0.619
5.34 ± 0.798
6.727 ± 0.727
3.467 ± 0.42
7.767 ± 1.32
2.739 ± 0.57
4.716 ± 0.627
5.617 ± 0.603
9.847 ± 0.544
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.462 ± 0.225
2.288 ± 0.337
4.993 ± 0.426
2.739 ± 0.336
6.657 ± 0.797
7.282 ± 0.634
5.895 ± 0.319
7.004 ± 0.387
1.63 ± 0.226
2.878 ± 0.448
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here