Maize yellow dwarf virus-RMV2

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Polerovirus; Maize yellow mosaic virus

Average proteome isoelectric point is 7.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A165G9A9|A0A165G9A9_9LUTE p5 OS=Maize yellow dwarf virus-RMV2 OX=1833824 PE=4 SV=1
MM1 pKa = 7.33YY2 pKa = 9.85YY3 pKa = 9.27WHH5 pKa = 7.62DD6 pKa = 3.83EE7 pKa = 4.19SWSKK11 pKa = 9.2EE12 pKa = 4.25TLSAGYY18 pKa = 8.96VQNDD22 pKa = 3.46SSRR25 pKa = 11.84ATPYY29 pKa = 10.56FLIPTVVGNYY39 pKa = 8.95KK40 pKa = 10.44VYY42 pKa = 10.37IEE44 pKa = 4.78CEE46 pKa = 3.72GFQAVKK52 pKa = 10.8AKK54 pKa = 10.66GGEE57 pKa = 4.32NNGKK61 pKa = 8.12MAGFITYY68 pKa = 10.0DD69 pKa = 3.48PQQAGWQAYY78 pKa = 7.54SWAGCTLSNYY88 pKa = 10.07RR89 pKa = 11.84ITDD92 pKa = 3.52GCVQAHH98 pKa = 7.54PDD100 pKa = 3.81LKK102 pKa = 11.51VNGCSFTKK110 pKa = 9.81GQCVEE115 pKa = 4.11RR116 pKa = 11.84DD117 pKa = 3.61VVISFDD123 pKa = 3.85LSVASEE129 pKa = 5.05GYY131 pKa = 8.45WALQAPPIEE140 pKa = 4.59KK141 pKa = 9.74TDD143 pKa = 3.37DD144 pKa = 3.33HH145 pKa = 7.69NFIVSYY151 pKa = 11.25GNYY154 pKa = 7.86TEE156 pKa = 6.16KK157 pKa = 10.44ILEE160 pKa = 4.04WGMVSISIDD169 pKa = 3.9EE170 pKa = 4.37INSSNDD176 pKa = 2.82AQRR179 pKa = 11.84VPNRR183 pKa = 11.84NKK185 pKa = 9.79DD186 pKa = 3.23TSEE189 pKa = 4.03PNRR192 pKa = 11.84LTDD195 pKa = 3.89FTNSASVGVTVTDD208 pKa = 3.9KK209 pKa = 11.36NLDD212 pKa = 3.85SEE214 pKa = 4.88PEE216 pKa = 4.06GQLVVQTPSLQTPANPVAEE235 pKa = 4.44PASKK239 pKa = 10.8VSAKK243 pKa = 9.83VVAWLNNPDD252 pKa = 4.17PEE254 pKa = 5.01SDD256 pKa = 3.73ADD258 pKa = 4.01APPPPTPWDD267 pKa = 3.48SSQVRR272 pKa = 11.84LRR274 pKa = 11.84SRR276 pKa = 11.84EE277 pKa = 3.87EE278 pKa = 3.88ANLEE282 pKa = 3.93PSPQNASPRR291 pKa = 11.84EE292 pKa = 4.15YY293 pKa = 10.24MNKK296 pKa = 9.34TYY298 pKa = 10.69EE299 pKa = 4.69DD300 pKa = 4.27DD301 pKa = 3.9VASQATKK308 pKa = 10.27TPTLSGGSLRR318 pKa = 11.84PRR320 pKa = 11.84LKK322 pKa = 9.45QTSDD326 pKa = 3.09SAKK329 pKa = 10.65DD330 pKa = 3.37NSSQVGSQTGTLTGGSLRR348 pKa = 11.84PSLQGGRR355 pKa = 11.84LASQQFRR362 pKa = 11.84MTTEE366 pKa = 3.44QAKK369 pKa = 10.4QYY371 pKa = 11.12DD372 pKa = 4.33LIKK375 pKa = 10.51RR376 pKa = 11.84SMGRR380 pKa = 11.84TRR382 pKa = 11.84ANEE385 pKa = 3.88YY386 pKa = 10.78YY387 pKa = 10.32EE388 pKa = 4.41EE389 pKa = 4.14CRR391 pKa = 11.84KK392 pKa = 10.01AAEE395 pKa = 4.43GSS397 pKa = 3.55

Molecular weight: 43.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A165G999|A0A165G999_9LUTE Movement protein OS=Maize yellow dwarf virus-RMV2 OX=1833824 PE=3 SV=1
MM1 pKa = 6.87NTGGRR6 pKa = 11.84NGRR9 pKa = 11.84RR10 pKa = 11.84ARR12 pKa = 11.84NRR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84ARR18 pKa = 11.84NNNRR22 pKa = 11.84AQPVVVVAANPRR34 pKa = 11.84RR35 pKa = 11.84GRR37 pKa = 11.84PRR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84PSGNTAGRR51 pKa = 11.84PGVRR55 pKa = 11.84RR56 pKa = 11.84GPRR59 pKa = 11.84EE60 pKa = 3.62TFVFSKK66 pKa = 11.02DD67 pKa = 3.43SLTGNASGKK76 pKa = 7.79VTFGPSLSEE85 pKa = 3.84CAAFSGGILKK95 pKa = 10.27AYY97 pKa = 10.08HH98 pKa = 6.52EE99 pKa = 4.39YY100 pKa = 10.12KK101 pKa = 10.44IKK103 pKa = 10.53IILEE107 pKa = 4.7FISEE111 pKa = 4.21AASTAEE117 pKa = 3.73GSIAYY122 pKa = 9.5EE123 pKa = 3.8LDD125 pKa = 3.19PHH127 pKa = 6.74NKK129 pKa = 9.68LSSLASTINKK139 pKa = 9.5FSIVKK144 pKa = 9.57GGRR147 pKa = 11.84RR148 pKa = 11.84VFASNQIGGGVWRR161 pKa = 11.84DD162 pKa = 3.39SSEE165 pKa = 4.18DD166 pKa = 3.19QCTIHH171 pKa = 6.52YY172 pKa = 9.09KK173 pKa = 11.03GNGKK177 pKa = 10.18SSVAGSFRR185 pKa = 11.84ITIEE189 pKa = 4.01VNVQNPRR196 pKa = 3.21

Molecular weight: 21.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2004

191

622

334.0

37.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.886 ± 0.41

1.747 ± 0.301

4.691 ± 0.711

5.439 ± 0.408

3.792 ± 0.731

6.986 ± 0.818

1.996 ± 0.412

3.992 ± 0.52

4.591 ± 0.573

9.481 ± 1.4

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.747 ± 0.328

4.341 ± 0.771

5.639 ± 0.685

4.341 ± 0.645

7.036 ± 1.441

10.08 ± 0.822

6.088 ± 0.59

6.487 ± 0.607

1.547 ± 0.235

3.094 ± 0.389
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski