Spiroplasma sp. ChiS

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Entomoplasmatales; Spiroplasmataceae; Spiroplasma; unclassified Spiroplasma

Average proteome isoelectric point is 7.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1511 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S8NRJ0|A0A2S8NRJ0_9MOLU Uncharacterized protein (Fragment) OS=Spiroplasma sp. ChiS OX=2099885 GN=C6B38_04890 PE=4 SV=1
MM1 pKa = 7.74IPQSLNNEE9 pKa = 3.88DD10 pKa = 4.18SIYY13 pKa = 10.45LINEE17 pKa = 4.05EE18 pKa = 4.62SVSLPTMEE26 pKa = 5.19SEE28 pKa = 4.74NLLTDD33 pKa = 3.55IVGSGRR39 pKa = 11.84YY40 pKa = 9.32IIDD43 pKa = 3.08AVGFYY48 pKa = 11.09NLGKK52 pKa = 7.1TACRR56 pKa = 11.84IYY58 pKa = 10.37MFSDD62 pKa = 4.78DD63 pKa = 3.65IDD65 pKa = 4.29IYY67 pKa = 11.42DD68 pKa = 4.48PYY70 pKa = 11.48SIMSYY75 pKa = 10.15FVSLQTTSLYY85 pKa = 10.89NNSVRR90 pKa = 11.84DD91 pKa = 3.56WLTLYY96 pKa = 10.76SLSYY100 pKa = 10.35LDD102 pKa = 5.35QYY104 pKa = 11.35IDD106 pKa = 4.64DD107 pKa = 6.02DD108 pKa = 4.68IVHH111 pKa = 7.27DD112 pKa = 4.58WPVKK116 pKa = 10.75LPDD119 pKa = 4.79PPPSINFYY127 pKa = 9.98PVQINLSEE135 pKa = 3.68ISAYY139 pKa = 10.8LKK141 pKa = 11.04LNSQNLNPKK150 pKa = 10.23SKK152 pKa = 10.74LNKK155 pKa = 9.03QKK157 pKa = 10.95LYY159 pKa = 10.89NN160 pKa = 3.64

Molecular weight: 18.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S8NRI9|A0A2S8NRI9_9MOLU DUF3627 domain-containing protein OS=Spiroplasma sp. ChiS OX=2099885 GN=C6B38_04840 PE=4 SV=1
MM1 pKa = 7.32KK2 pKa = 9.47RR3 pKa = 11.84TWQPSKK9 pKa = 10.14IKK11 pKa = 10.33HH12 pKa = 5.53KK13 pKa = 9.07RR14 pKa = 11.84THH16 pKa = 5.81GFRR19 pKa = 11.84ARR21 pKa = 11.84MEE23 pKa = 4.26SASGRR28 pKa = 11.84KK29 pKa = 9.01VLSKK33 pKa = 10.58RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.82GRR39 pKa = 11.84KK40 pKa = 8.62VLSAA44 pKa = 4.05

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1511

0

1511

341352

16

1454

225.9

26.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.088 ± 0.075

0.857 ± 0.02

4.992 ± 0.057

6.166 ± 0.075

4.993 ± 0.066

4.812 ± 0.069

1.589 ± 0.031

9.862 ± 0.072

9.64 ± 0.082

9.826 ± 0.071

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.152 ± 0.027

7.701 ± 0.09

2.853 ± 0.036

3.996 ± 0.048

3.106 ± 0.052

5.312 ± 0.047

5.774 ± 0.052

5.477 ± 0.059

1.313 ± 0.034

4.451 ± 0.054
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski