Alkalicaulis satelles

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Maricaulales; Maricaulaceae; Alkalicaulis

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2694 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5M6Z9K1|A0A5M6Z9K1_9PROT Glutathione reductase OS=Alkalicaulis satelles OX=2609175 GN=gor PE=3 SV=1
MM1 pKa = 7.97RR2 pKa = 11.84ISTLLASSVLAFGAVGAAAVADD24 pKa = 3.81HH25 pKa = 6.95HH26 pKa = 7.04GDD28 pKa = 3.6GYY30 pKa = 10.81SAEE33 pKa = 4.11AQPTIVEE40 pKa = 4.23IASADD45 pKa = 3.66DD46 pKa = 3.67TFSTLVAAVTAADD59 pKa = 4.5LADD62 pKa = 3.78ALAGEE67 pKa = 5.13GPFTVFAPTNDD78 pKa = 3.5AFDD81 pKa = 4.64ALPEE85 pKa = 4.19GTVEE89 pKa = 4.54SLLEE93 pKa = 4.13EE94 pKa = 4.54GNRR97 pKa = 11.84DD98 pKa = 3.2QLTAVLTYY106 pKa = 9.87HH107 pKa = 5.74VVSGRR112 pKa = 11.84YY113 pKa = 8.39LAAEE117 pKa = 4.53TPAGTYY123 pKa = 7.46EE124 pKa = 4.32LPTLQGSTVTVVIGEE139 pKa = 4.44DD140 pKa = 3.53GAVTVDD146 pKa = 3.43GANVISADD154 pKa = 3.34IEE156 pKa = 4.06ASNGVIHH163 pKa = 7.02VIDD166 pKa = 4.33AVILPP171 pKa = 4.22

Molecular weight: 17.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5M6ZL28|A0A5M6ZL28_9PROT Uncharacterized protein OS=Alkalicaulis satelles OX=2609175 GN=F1654_03215 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 9.58LVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.38GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.29SGRR28 pKa = 11.84LVIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.91GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2694

0

2694

872582

26

2105

323.9

34.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.8 ± 0.088

0.785 ± 0.014

6.003 ± 0.039

6.11 ± 0.039

3.615 ± 0.032

8.949 ± 0.045

1.968 ± 0.021

4.554 ± 0.033

2.336 ± 0.04

10.069 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.391 ± 0.022

2.157 ± 0.035

5.361 ± 0.037

2.928 ± 0.022

7.983 ± 0.049

5.019 ± 0.033

4.672 ± 0.033

6.837 ± 0.032

1.427 ± 0.02

2.039 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski