Black raspberry virus F

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Chrymotiviricetes; Ghabrivirales; Totiviridae; Totivirus; unclassified Totivirus

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A7XMH8|A7XMH8_9VIRU RNA-directed RNA polymerase OS=Black raspberry virus F OX=463392 PE=3 SV=1
MM1 pKa = 8.1DD2 pKa = 5.44AFLKK6 pKa = 10.79KK7 pKa = 10.37FFEE10 pKa = 4.71GFEE13 pKa = 4.1APGAEE18 pKa = 4.21FSFNASPARR27 pKa = 11.84IYY29 pKa = 10.9TFFQSLLRR37 pKa = 11.84VDD39 pKa = 4.25CDD41 pKa = 3.24GVIQNRR47 pKa = 11.84PFYY50 pKa = 10.38VNQPMTPLGKK60 pKa = 8.0MTARR64 pKa = 11.84FGSTVSTLDD73 pKa = 4.6GISKK77 pKa = 10.15PYY79 pKa = 8.72LTPEE83 pKa = 3.91GVINLNAIGDD93 pKa = 4.14VLKK96 pKa = 10.16STGGMTSNAWSLHH109 pKa = 4.7MGVVKK114 pKa = 10.32GWNWADD120 pKa = 3.11NHH122 pKa = 6.28VPLLVNMLRR131 pKa = 11.84YY132 pKa = 8.07TLIKK136 pKa = 9.98HH137 pKa = 5.45IQTVAGTLDD146 pKa = 3.67ATLGTYY152 pKa = 10.67DD153 pKa = 4.94DD154 pKa = 3.92GHH156 pKa = 5.49VTINSDD162 pKa = 3.56GCFTEE167 pKa = 5.89AYY169 pKa = 9.01PEE171 pKa = 3.78EE172 pKa = 4.48AAWDD176 pKa = 3.85GWPFAVPEE184 pKa = 4.21GQAAIEE190 pKa = 4.2AWTQFTRR197 pKa = 11.84FEE199 pKa = 4.17TAEE202 pKa = 3.89PQANDD207 pKa = 2.68VCLDD211 pKa = 3.7LRR213 pKa = 11.84NLTPQEE219 pKa = 4.2SAFVLLMCTQWQRR232 pKa = 11.84KK233 pKa = 7.32SRR235 pKa = 11.84LRR237 pKa = 11.84LDD239 pKa = 3.7FALPEE244 pKa = 4.08LAHH247 pKa = 6.66AISYY251 pKa = 8.6RR252 pKa = 11.84ASAEE256 pKa = 3.89PAGFAGWLSGTYY268 pKa = 9.58VAAQGPPAAPGPEE281 pKa = 4.26LAWSALKK288 pKa = 10.5KK289 pKa = 10.45YY290 pKa = 10.44VNQNRR295 pKa = 11.84LYY297 pKa = 10.9DD298 pKa = 3.77HH299 pKa = 7.26FSTALYY305 pKa = 10.45LVACSMYY312 pKa = 10.49QFAPVTAEE320 pKa = 3.55AHH322 pKa = 5.59SWLDD326 pKa = 3.41VDD328 pKa = 4.52WDD330 pKa = 3.79LSLPLFHH337 pKa = 7.0SVRR340 pKa = 11.84GRR342 pKa = 11.84YY343 pKa = 6.99TFLNEE348 pKa = 4.18DD349 pKa = 3.29EE350 pKa = 4.78AAFISHH356 pKa = 7.21RR357 pKa = 11.84ALNEE361 pKa = 3.28WSYY364 pKa = 11.97YY365 pKa = 9.3SVNINRR371 pKa = 11.84INLLALTFSQAYY383 pKa = 6.48HH384 pKa = 6.04TGFAVRR390 pKa = 11.84NVRR393 pKa = 11.84KK394 pKa = 10.23SITGKK399 pKa = 10.49LDD401 pKa = 4.65DD402 pKa = 4.47IYY404 pKa = 9.28TTEE407 pKa = 4.51GEE409 pKa = 4.22LLAPISMIPSAASEE423 pKa = 4.19AVRR426 pKa = 11.84APVPLSGMSGVYY438 pKa = 9.26MYY440 pKa = 9.47TQYY443 pKa = 11.97NLLDD447 pKa = 3.87YY448 pKa = 10.92DD449 pKa = 4.08PNRR452 pKa = 11.84TITLEE457 pKa = 3.66NDD459 pKa = 3.25YY460 pKa = 10.64TGEE463 pKa = 4.27DD464 pKa = 3.45DD465 pKa = 5.73GYY467 pKa = 11.43DD468 pKa = 3.64LIEE471 pKa = 4.7PEE473 pKa = 4.05VTTRR477 pKa = 11.84TEE479 pKa = 3.78VTVRR483 pKa = 11.84AGVDD487 pKa = 3.58LNALLLGGPPAAGTRR502 pKa = 11.84GGNNAAARR510 pKa = 11.84GRR512 pKa = 11.84GLGRR516 pKa = 11.84GGRR519 pKa = 11.84NGGRR523 pKa = 11.84GNGSTSAEE531 pKa = 4.06AEE533 pKa = 4.35DD534 pKa = 4.35GDD536 pKa = 4.68EE537 pKa = 5.63NLTIDD542 pKa = 4.43NFDD545 pKa = 4.14PNDD548 pKa = 3.38YY549 pKa = 10.89FEE551 pKa = 4.38VVEE554 pKa = 4.21IEE556 pKa = 4.44EE557 pKa = 4.33PAGPTTLRR565 pKa = 11.84VPWMTFPGYY574 pKa = 8.27PTMLVPIDD582 pKa = 4.47PFPDD586 pKa = 3.36VNIFTLKK593 pKa = 10.75GKK595 pKa = 10.02IDD597 pKa = 4.07GTTGDD602 pKa = 3.7CTRR605 pKa = 11.84WGFKK609 pKa = 6.56TTAMRR614 pKa = 11.84AWYY617 pKa = 9.28VANLYY622 pKa = 9.81RR623 pKa = 11.84LCGYY627 pKa = 10.69DD628 pKa = 4.44LDD630 pKa = 5.08FSSAQNLVGGEE641 pKa = 4.0KK642 pKa = 9.98FYY644 pKa = 11.51APNNVGFVWPILYY657 pKa = 9.76EE658 pKa = 4.31PNHH661 pKa = 5.26QHH663 pKa = 7.25DD664 pKa = 4.97EE665 pKa = 4.06IVINYY670 pKa = 6.31QQQRR674 pKa = 11.84MHH676 pKa = 6.75HH677 pKa = 7.22FITLPQIINNFFKK690 pKa = 11.06NKK692 pKa = 9.14TIEE695 pKa = 3.92FSHH698 pKa = 6.92RR699 pKa = 11.84IIRR702 pKa = 11.84RR703 pKa = 11.84GTTTSAYY710 pKa = 10.3GNWLDD715 pKa = 3.68VVEE718 pKa = 4.51SSGVVQMFANMTVAYY733 pKa = 7.14NTSAAVSRR741 pKa = 11.84LRR743 pKa = 11.84AYY745 pKa = 8.52ITRR748 pKa = 11.84SEE750 pKa = 3.99EE751 pKa = 3.64GFRR754 pKa = 11.84FVDD757 pKa = 3.72NVQGGAIPPPTEE769 pKa = 3.49

Molecular weight:
85.29 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A7XMH8|A7XMH8_9VIRU RNA-directed RNA polymerase OS=Black raspberry virus F OX=463392 PE=3 SV=1
MM1 pKa = 7.67NIPVWVTIRR10 pKa = 11.84GEE12 pKa = 4.04CLTAVDD18 pKa = 4.25RR19 pKa = 11.84EE20 pKa = 4.02KK21 pKa = 11.2AKK23 pKa = 10.93YY24 pKa = 9.16VLAHH28 pKa = 5.86VSYY31 pKa = 11.41SNTQDD36 pKa = 3.2SPGFGMFRR44 pKa = 11.84VGKK47 pKa = 8.5EE48 pKa = 3.91CVKK51 pKa = 10.94GFMARR56 pKa = 11.84CEE58 pKa = 4.86DD59 pKa = 2.73IWVYY63 pKa = 8.79YY64 pKa = 10.76VKK66 pKa = 10.09TSTPAISLPPVVKK79 pKa = 9.42RR80 pKa = 11.84TMSAMYY86 pKa = 10.19SGVEE90 pKa = 4.5DD91 pKa = 3.88YY92 pKa = 11.33DD93 pKa = 4.37FSDD96 pKa = 3.68FRR98 pKa = 11.84GHH100 pKa = 6.71RR101 pKa = 11.84FLRR104 pKa = 11.84RR105 pKa = 11.84KK106 pKa = 9.64FGVVRR111 pKa = 11.84EE112 pKa = 4.36EE113 pKa = 4.2IIHH116 pKa = 6.37AKK118 pKa = 9.74NIKK121 pKa = 9.33PPQKK125 pKa = 11.05GEE127 pKa = 4.0FEE129 pKa = 4.05KK130 pKa = 11.23SKK132 pKa = 9.34ITGEE136 pKa = 4.05HH137 pKa = 5.61HH138 pKa = 4.68THH140 pKa = 5.11YY141 pKa = 10.08TPDD144 pKa = 4.02EE145 pKa = 4.01VWEE148 pKa = 4.22IAVQTNTMRR157 pKa = 11.84RR158 pKa = 11.84ALLLVVDD165 pKa = 4.79KK166 pKa = 10.96LRR168 pKa = 11.84EE169 pKa = 3.85ISGVTEE175 pKa = 5.09AIVSTFMAYY184 pKa = 9.55IMFAKK189 pKa = 10.01PQVAYY194 pKa = 11.05LLATSANIWRR204 pKa = 11.84AKK206 pKa = 10.16DD207 pKa = 3.49VNTLAEE213 pKa = 4.15KK214 pKa = 10.46LKK216 pKa = 10.77EE217 pKa = 3.57ISTPLKK223 pKa = 10.14SMHH226 pKa = 6.21RR227 pKa = 11.84HH228 pKa = 6.2DD229 pKa = 4.61VLDD232 pKa = 3.54MTQLFEE238 pKa = 4.69LQVLVNRR245 pKa = 11.84GIGKK249 pKa = 9.49VDD251 pKa = 3.04WVNEE255 pKa = 3.94RR256 pKa = 11.84KK257 pKa = 9.44HH258 pKa = 5.46RR259 pKa = 11.84QKK261 pKa = 11.02PDD263 pKa = 3.05TVKK266 pKa = 10.95VGFTDD271 pKa = 4.3VYY273 pKa = 10.95CEE275 pKa = 3.71ARR277 pKa = 11.84RR278 pKa = 11.84LFEE281 pKa = 5.42LGVQRR286 pKa = 11.84GFKK289 pKa = 10.1YY290 pKa = 8.7PTMDD294 pKa = 3.09FDD296 pKa = 5.31KK297 pKa = 10.54YY298 pKa = 11.1LKK300 pKa = 10.54SRR302 pKa = 11.84WEE304 pKa = 3.9WVPTGSVHH312 pKa = 6.1SQYY315 pKa = 11.2EE316 pKa = 3.96GDD318 pKa = 3.43QEE320 pKa = 5.0YY321 pKa = 10.14IAKK324 pKa = 10.11DD325 pKa = 3.27YY326 pKa = 10.7RR327 pKa = 11.84HH328 pKa = 6.22RR329 pKa = 11.84SKK331 pKa = 10.89FVTLNRR337 pKa = 11.84LSCDD341 pKa = 3.5QIRR344 pKa = 11.84EE345 pKa = 4.05MFNRR349 pKa = 11.84KK350 pKa = 9.08PEE352 pKa = 3.87IQAWASVKK360 pKa = 10.46YY361 pKa = 9.66EE362 pKa = 3.86WAKK365 pKa = 10.25QRR367 pKa = 11.84AIYY370 pKa = 10.09GVDD373 pKa = 3.36LTSSVITNYY382 pKa = 11.26AMFRR386 pKa = 11.84CEE388 pKa = 4.22DD389 pKa = 3.64VLRR392 pKa = 11.84HH393 pKa = 5.83HH394 pKa = 6.86FPIGSEE400 pKa = 3.48ADD402 pKa = 3.09AQRR405 pKa = 11.84VHH407 pKa = 6.98KK408 pKa = 10.43RR409 pKa = 11.84LTYY412 pKa = 9.07MLKK415 pKa = 9.3DD416 pKa = 3.76TEE418 pKa = 4.51SFCYY422 pKa = 10.73DD423 pKa = 3.11FDD425 pKa = 5.14DD426 pKa = 5.75FNAQHH431 pKa = 6.54SIEE434 pKa = 4.25SMEE437 pKa = 4.27AVLLAYY443 pKa = 10.3YY444 pKa = 10.27DD445 pKa = 3.59QFKK448 pKa = 11.09DD449 pKa = 3.98QMTAAQASAMEE460 pKa = 4.44WVCQSVKK467 pKa = 10.84EE468 pKa = 4.03MIVNNNEE475 pKa = 3.64VQPPEE480 pKa = 4.08KK481 pKa = 10.46YY482 pKa = 9.95KK483 pKa = 11.27LKK485 pKa = 9.87GTLLSGWRR493 pKa = 11.84LTTFMNTVLNYY504 pKa = 9.71IYY506 pKa = 10.52FRR508 pKa = 11.84ISGALKK514 pKa = 9.47TPDD517 pKa = 3.27VVDD520 pKa = 4.09SVHH523 pKa = 6.92NGDD526 pKa = 5.82DD527 pKa = 3.52VLLSINNLKK536 pKa = 10.62AAVEE540 pKa = 4.14VDD542 pKa = 2.9SRR544 pKa = 11.84MAKK547 pKa = 9.82IGARR551 pKa = 11.84AQAAKK556 pKa = 10.58CNVFSIGEE564 pKa = 4.03FLRR567 pKa = 11.84VDD569 pKa = 3.4HH570 pKa = 6.91KK571 pKa = 10.73ISRR574 pKa = 11.84EE575 pKa = 3.97KK576 pKa = 11.01GVGAQYY582 pKa = 11.01LSRR585 pKa = 11.84AAATLVHH592 pKa = 6.53SRR594 pKa = 11.84IEE596 pKa = 4.15SQAPLRR602 pKa = 11.84LIEE605 pKa = 4.28AVKK608 pKa = 10.26AAYY611 pKa = 8.36TRR613 pKa = 11.84ASEE616 pKa = 3.97VAMRR620 pKa = 11.84SDD622 pKa = 3.26QSSEE626 pKa = 3.11ISALFFHH633 pKa = 7.16KK634 pKa = 10.33AAKK637 pKa = 9.65RR638 pKa = 11.84LSSIFGVDD646 pKa = 3.42YY647 pKa = 10.91EE648 pKa = 4.54KK649 pKa = 11.29VRR651 pKa = 11.84IAANQHH657 pKa = 4.76VVTGGIRR664 pKa = 11.84QDD666 pKa = 3.34FMASVEE672 pKa = 4.07YY673 pKa = 10.43LIHH676 pKa = 6.84EE677 pKa = 4.19EE678 pKa = 4.17VEE680 pKa = 4.19RR681 pKa = 11.84EE682 pKa = 4.0KK683 pKa = 11.31LGTDD687 pKa = 3.21SLHH690 pKa = 6.88NLGQVDD696 pKa = 4.04VSDD699 pKa = 4.12LGEE702 pKa = 4.42GIKK705 pKa = 10.51DD706 pKa = 3.61YY707 pKa = 11.47GSVLMSQYY715 pKa = 11.2GKK717 pKa = 8.83YY718 pKa = 5.92TTEE721 pKa = 3.3EE722 pKa = 4.2RR723 pKa = 11.84IYY725 pKa = 10.97KK726 pKa = 10.19SIVSATGRR734 pKa = 11.84QLSITRR740 pKa = 11.84STRR743 pKa = 11.84LSVVDD748 pKa = 3.69VRR750 pKa = 11.84HH751 pKa = 5.42DD752 pKa = 3.34TKK754 pKa = 11.11YY755 pKa = 10.12GYY757 pKa = 10.8GRR759 pKa = 11.84ALYY762 pKa = 9.89GYY764 pKa = 10.07LRR766 pKa = 11.84NLVNLPYY773 pKa = 10.08VEE775 pKa = 4.26KK776 pKa = 10.83ARR778 pKa = 11.84FVGISPLAMLDD789 pKa = 3.47AKK791 pKa = 11.34GMGTLRR797 pKa = 11.84KK798 pKa = 9.39YY799 pKa = 11.09IMGASDD805 pKa = 3.03VDD807 pKa = 3.51YY808 pKa = 10.28TLRR811 pKa = 11.84VLLL814 pKa = 4.58

Molecular weight:
93.03 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1583

769

814

791.5

89.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.528 ± 0.613

1.011 ± 0.074

5.559 ± 0.072

5.938 ± 0.35

4.548 ± 0.48

6.822 ± 1.006

2.401 ± 0.33

4.864 ± 0.23

4.611 ± 1.668

7.96 ± 0.267

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.716 ± 0.371

4.422 ± 1.05

4.169 ± 1.235

3.285 ± 0.216

6.128 ± 0.68

6.254 ± 0.582

6.507 ± 0.856

7.833 ± 1.073

1.769 ± 0.324

4.675 ± 0.186

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski