Black raspberry virus F
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A7XMH8|A7XMH8_9VIRU RNA-directed RNA polymerase OS=Black raspberry virus F OX=463392 PE=3 SV=1
MM1 pKa = 8.1 DD2 pKa = 5.44 AFLKK6 pKa = 10.79 KK7 pKa = 10.37 FFEE10 pKa = 4.71 GFEE13 pKa = 4.1 APGAEE18 pKa = 4.21 FSFNASPARR27 pKa = 11.84 IYY29 pKa = 10.9 TFFQSLLRR37 pKa = 11.84 VDD39 pKa = 4.25 CDD41 pKa = 3.24 GVIQNRR47 pKa = 11.84 PFYY50 pKa = 10.38 VNQPMTPLGKK60 pKa = 8.0 MTARR64 pKa = 11.84 FGSTVSTLDD73 pKa = 4.6 GISKK77 pKa = 10.15 PYY79 pKa = 8.72 LTPEE83 pKa = 3.91 GVINLNAIGDD93 pKa = 4.14 VLKK96 pKa = 10.16 STGGMTSNAWSLHH109 pKa = 4.7 MGVVKK114 pKa = 10.32 GWNWADD120 pKa = 3.11 NHH122 pKa = 6.28 VPLLVNMLRR131 pKa = 11.84 YY132 pKa = 8.07 TLIKK136 pKa = 9.98 HH137 pKa = 5.45 IQTVAGTLDD146 pKa = 3.67 ATLGTYY152 pKa = 10.67 DD153 pKa = 4.94 DD154 pKa = 3.92 GHH156 pKa = 5.49 VTINSDD162 pKa = 3.56 GCFTEE167 pKa = 5.89 AYY169 pKa = 9.01 PEE171 pKa = 3.78 EE172 pKa = 4.48 AAWDD176 pKa = 3.85 GWPFAVPEE184 pKa = 4.21 GQAAIEE190 pKa = 4.2 AWTQFTRR197 pKa = 11.84 FEE199 pKa = 4.17 TAEE202 pKa = 3.89 PQANDD207 pKa = 2.68 VCLDD211 pKa = 3.7 LRR213 pKa = 11.84 NLTPQEE219 pKa = 4.2 SAFVLLMCTQWQRR232 pKa = 11.84 KK233 pKa = 7.32 SRR235 pKa = 11.84 LRR237 pKa = 11.84 LDD239 pKa = 3.7 FALPEE244 pKa = 4.08 LAHH247 pKa = 6.66 AISYY251 pKa = 8.6 RR252 pKa = 11.84 ASAEE256 pKa = 3.89 PAGFAGWLSGTYY268 pKa = 9.58 VAAQGPPAAPGPEE281 pKa = 4.26 LAWSALKK288 pKa = 10.5 KK289 pKa = 10.45 YY290 pKa = 10.44 VNQNRR295 pKa = 11.84 LYY297 pKa = 10.9 DD298 pKa = 3.77 HH299 pKa = 7.26 FSTALYY305 pKa = 10.45 LVACSMYY312 pKa = 10.49 QFAPVTAEE320 pKa = 3.55 AHH322 pKa = 5.59 SWLDD326 pKa = 3.41 VDD328 pKa = 4.52 WDD330 pKa = 3.79 LSLPLFHH337 pKa = 7.0 SVRR340 pKa = 11.84 GRR342 pKa = 11.84 YY343 pKa = 6.99 TFLNEE348 pKa = 4.18 DD349 pKa = 3.29 EE350 pKa = 4.78 AAFISHH356 pKa = 7.21 RR357 pKa = 11.84 ALNEE361 pKa = 3.28 WSYY364 pKa = 11.97 YY365 pKa = 9.3 SVNINRR371 pKa = 11.84 INLLALTFSQAYY383 pKa = 6.48 HH384 pKa = 6.04 TGFAVRR390 pKa = 11.84 NVRR393 pKa = 11.84 KK394 pKa = 10.23 SITGKK399 pKa = 10.49 LDD401 pKa = 4.65 DD402 pKa = 4.47 IYY404 pKa = 9.28 TTEE407 pKa = 4.51 GEE409 pKa = 4.22 LLAPISMIPSAASEE423 pKa = 4.19 AVRR426 pKa = 11.84 APVPLSGMSGVYY438 pKa = 9.26 MYY440 pKa = 9.47 TQYY443 pKa = 11.97 NLLDD447 pKa = 3.87 YY448 pKa = 10.92 DD449 pKa = 4.08 PNRR452 pKa = 11.84 TITLEE457 pKa = 3.66 NDD459 pKa = 3.25 YY460 pKa = 10.64 TGEE463 pKa = 4.27 DD464 pKa = 3.45 DD465 pKa = 5.73 GYY467 pKa = 11.43 DD468 pKa = 3.64 LIEE471 pKa = 4.7 PEE473 pKa = 4.05 VTTRR477 pKa = 11.84 TEE479 pKa = 3.78 VTVRR483 pKa = 11.84 AGVDD487 pKa = 3.58 LNALLLGGPPAAGTRR502 pKa = 11.84 GGNNAAARR510 pKa = 11.84 GRR512 pKa = 11.84 GLGRR516 pKa = 11.84 GGRR519 pKa = 11.84 NGGRR523 pKa = 11.84 GNGSTSAEE531 pKa = 4.06 AEE533 pKa = 4.35 DD534 pKa = 4.35 GDD536 pKa = 4.68 EE537 pKa = 5.63 NLTIDD542 pKa = 4.43 NFDD545 pKa = 4.14 PNDD548 pKa = 3.38 YY549 pKa = 10.89 FEE551 pKa = 4.38 VVEE554 pKa = 4.21 IEE556 pKa = 4.44 EE557 pKa = 4.33 PAGPTTLRR565 pKa = 11.84 VPWMTFPGYY574 pKa = 8.27 PTMLVPIDD582 pKa = 4.47 PFPDD586 pKa = 3.36 VNIFTLKK593 pKa = 10.75 GKK595 pKa = 10.02 IDD597 pKa = 4.07 GTTGDD602 pKa = 3.7 CTRR605 pKa = 11.84 WGFKK609 pKa = 6.56 TTAMRR614 pKa = 11.84 AWYY617 pKa = 9.28 VANLYY622 pKa = 9.81 RR623 pKa = 11.84 LCGYY627 pKa = 10.69 DD628 pKa = 4.44 LDD630 pKa = 5.08 FSSAQNLVGGEE641 pKa = 4.0 KK642 pKa = 9.98 FYY644 pKa = 11.51 APNNVGFVWPILYY657 pKa = 9.76 EE658 pKa = 4.31 PNHH661 pKa = 5.26 QHH663 pKa = 7.25 DD664 pKa = 4.97 EE665 pKa = 4.06 IVINYY670 pKa = 6.31 QQQRR674 pKa = 11.84 MHH676 pKa = 6.75 HH677 pKa = 7.22 FITLPQIINNFFKK690 pKa = 11.06 NKK692 pKa = 9.14 TIEE695 pKa = 3.92 FSHH698 pKa = 6.92 RR699 pKa = 11.84 IIRR702 pKa = 11.84 RR703 pKa = 11.84 GTTTSAYY710 pKa = 10.3 GNWLDD715 pKa = 3.68 VVEE718 pKa = 4.51 SSGVVQMFANMTVAYY733 pKa = 7.14 NTSAAVSRR741 pKa = 11.84 LRR743 pKa = 11.84 AYY745 pKa = 8.52 ITRR748 pKa = 11.84 SEE750 pKa = 3.99 EE751 pKa = 3.64 GFRR754 pKa = 11.84 FVDD757 pKa = 3.72 NVQGGAIPPPTEE769 pKa = 3.49
Molecular weight: 85.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.866
IPC2_protein 4.952
IPC_protein 4.914
Toseland 4.838
ProMoST 5.029
Dawson 4.902
Bjellqvist 5.054
Wikipedia 4.787
Rodwell 4.813
Grimsley 4.762
Solomon 4.902
Lehninger 4.863
Nozaki 5.029
DTASelect 5.194
Thurlkill 4.838
EMBOSS 4.825
Sillero 5.092
Patrickios 3.63
IPC_peptide 4.914
IPC2_peptide 5.092
IPC2.peptide.svr19 5.003
Protein with the highest isoelectric point:
>tr|A7XMH8|A7XMH8_9VIRU RNA-directed RNA polymerase OS=Black raspberry virus F OX=463392 PE=3 SV=1
MM1 pKa = 7.67 NIPVWVTIRR10 pKa = 11.84 GEE12 pKa = 4.04 CLTAVDD18 pKa = 4.25 RR19 pKa = 11.84 EE20 pKa = 4.02 KK21 pKa = 11.2 AKK23 pKa = 10.93 YY24 pKa = 9.16 VLAHH28 pKa = 5.86 VSYY31 pKa = 11.41 SNTQDD36 pKa = 3.2 SPGFGMFRR44 pKa = 11.84 VGKK47 pKa = 8.5 EE48 pKa = 3.91 CVKK51 pKa = 10.94 GFMARR56 pKa = 11.84 CEE58 pKa = 4.86 DD59 pKa = 2.73 IWVYY63 pKa = 8.79 YY64 pKa = 10.76 VKK66 pKa = 10.09 TSTPAISLPPVVKK79 pKa = 9.42 RR80 pKa = 11.84 TMSAMYY86 pKa = 10.19 SGVEE90 pKa = 4.5 DD91 pKa = 3.88 YY92 pKa = 11.33 DD93 pKa = 4.37 FSDD96 pKa = 3.68 FRR98 pKa = 11.84 GHH100 pKa = 6.71 RR101 pKa = 11.84 FLRR104 pKa = 11.84 RR105 pKa = 11.84 KK106 pKa = 9.64 FGVVRR111 pKa = 11.84 EE112 pKa = 4.36 EE113 pKa = 4.2 IIHH116 pKa = 6.37 AKK118 pKa = 9.74 NIKK121 pKa = 9.33 PPQKK125 pKa = 11.05 GEE127 pKa = 4.0 FEE129 pKa = 4.05 KK130 pKa = 11.23 SKK132 pKa = 9.34 ITGEE136 pKa = 4.05 HH137 pKa = 5.61 HH138 pKa = 4.68 THH140 pKa = 5.11 YY141 pKa = 10.08 TPDD144 pKa = 4.02 EE145 pKa = 4.01 VWEE148 pKa = 4.22 IAVQTNTMRR157 pKa = 11.84 RR158 pKa = 11.84 ALLLVVDD165 pKa = 4.79 KK166 pKa = 10.96 LRR168 pKa = 11.84 EE169 pKa = 3.85 ISGVTEE175 pKa = 5.09 AIVSTFMAYY184 pKa = 9.55 IMFAKK189 pKa = 10.01 PQVAYY194 pKa = 11.05 LLATSANIWRR204 pKa = 11.84 AKK206 pKa = 10.16 DD207 pKa = 3.49 VNTLAEE213 pKa = 4.15 KK214 pKa = 10.46 LKK216 pKa = 10.77 EE217 pKa = 3.57 ISTPLKK223 pKa = 10.14 SMHH226 pKa = 6.21 RR227 pKa = 11.84 HH228 pKa = 6.2 DD229 pKa = 4.61 VLDD232 pKa = 3.54 MTQLFEE238 pKa = 4.69 LQVLVNRR245 pKa = 11.84 GIGKK249 pKa = 9.49 VDD251 pKa = 3.04 WVNEE255 pKa = 3.94 RR256 pKa = 11.84 KK257 pKa = 9.44 HH258 pKa = 5.46 RR259 pKa = 11.84 QKK261 pKa = 11.02 PDD263 pKa = 3.05 TVKK266 pKa = 10.95 VGFTDD271 pKa = 4.3 VYY273 pKa = 10.95 CEE275 pKa = 3.71 ARR277 pKa = 11.84 RR278 pKa = 11.84 LFEE281 pKa = 5.42 LGVQRR286 pKa = 11.84 GFKK289 pKa = 10.1 YY290 pKa = 8.7 PTMDD294 pKa = 3.09 FDD296 pKa = 5.31 KK297 pKa = 10.54 YY298 pKa = 11.1 LKK300 pKa = 10.54 SRR302 pKa = 11.84 WEE304 pKa = 3.9 WVPTGSVHH312 pKa = 6.1 SQYY315 pKa = 11.2 EE316 pKa = 3.96 GDD318 pKa = 3.43 QEE320 pKa = 5.0 YY321 pKa = 10.14 IAKK324 pKa = 10.11 DD325 pKa = 3.27 YY326 pKa = 10.7 RR327 pKa = 11.84 HH328 pKa = 6.22 RR329 pKa = 11.84 SKK331 pKa = 10.89 FVTLNRR337 pKa = 11.84 LSCDD341 pKa = 3.5 QIRR344 pKa = 11.84 EE345 pKa = 4.05 MFNRR349 pKa = 11.84 KK350 pKa = 9.08 PEE352 pKa = 3.87 IQAWASVKK360 pKa = 10.46 YY361 pKa = 9.66 EE362 pKa = 3.86 WAKK365 pKa = 10.25 QRR367 pKa = 11.84 AIYY370 pKa = 10.09 GVDD373 pKa = 3.36 LTSSVITNYY382 pKa = 11.26 AMFRR386 pKa = 11.84 CEE388 pKa = 4.22 DD389 pKa = 3.64 VLRR392 pKa = 11.84 HH393 pKa = 5.83 HH394 pKa = 6.86 FPIGSEE400 pKa = 3.48 ADD402 pKa = 3.09 AQRR405 pKa = 11.84 VHH407 pKa = 6.98 KK408 pKa = 10.43 RR409 pKa = 11.84 LTYY412 pKa = 9.07 MLKK415 pKa = 9.3 DD416 pKa = 3.76 TEE418 pKa = 4.51 SFCYY422 pKa = 10.73 DD423 pKa = 3.11 FDD425 pKa = 5.14 DD426 pKa = 5.75 FNAQHH431 pKa = 6.54 SIEE434 pKa = 4.25 SMEE437 pKa = 4.27 AVLLAYY443 pKa = 10.3 YY444 pKa = 10.27 DD445 pKa = 3.59 QFKK448 pKa = 11.09 DD449 pKa = 3.98 QMTAAQASAMEE460 pKa = 4.44 WVCQSVKK467 pKa = 10.84 EE468 pKa = 4.03 MIVNNNEE475 pKa = 3.64 VQPPEE480 pKa = 4.08 KK481 pKa = 10.46 YY482 pKa = 9.95 KK483 pKa = 11.27 LKK485 pKa = 9.87 GTLLSGWRR493 pKa = 11.84 LTTFMNTVLNYY504 pKa = 9.71 IYY506 pKa = 10.52 FRR508 pKa = 11.84 ISGALKK514 pKa = 9.47 TPDD517 pKa = 3.27 VVDD520 pKa = 4.09 SVHH523 pKa = 6.92 NGDD526 pKa = 5.82 DD527 pKa = 3.52 VLLSINNLKK536 pKa = 10.62 AAVEE540 pKa = 4.14 VDD542 pKa = 2.9 SRR544 pKa = 11.84 MAKK547 pKa = 9.82 IGARR551 pKa = 11.84 AQAAKK556 pKa = 10.58 CNVFSIGEE564 pKa = 4.03 FLRR567 pKa = 11.84 VDD569 pKa = 3.4 HH570 pKa = 6.91 KK571 pKa = 10.73 ISRR574 pKa = 11.84 EE575 pKa = 3.97 KK576 pKa = 11.01 GVGAQYY582 pKa = 11.01 LSRR585 pKa = 11.84 AAATLVHH592 pKa = 6.53 SRR594 pKa = 11.84 IEE596 pKa = 4.15 SQAPLRR602 pKa = 11.84 LIEE605 pKa = 4.28 AVKK608 pKa = 10.26 AAYY611 pKa = 8.36 TRR613 pKa = 11.84 ASEE616 pKa = 3.97 VAMRR620 pKa = 11.84 SDD622 pKa = 3.26 QSSEE626 pKa = 3.11 ISALFFHH633 pKa = 7.16 KK634 pKa = 10.33 AAKK637 pKa = 9.65 RR638 pKa = 11.84 LSSIFGVDD646 pKa = 3.42 YY647 pKa = 10.91 EE648 pKa = 4.54 KK649 pKa = 11.29 VRR651 pKa = 11.84 IAANQHH657 pKa = 4.76 VVTGGIRR664 pKa = 11.84 QDD666 pKa = 3.34 FMASVEE672 pKa = 4.07 YY673 pKa = 10.43 LIHH676 pKa = 6.84 EE677 pKa = 4.19 EE678 pKa = 4.17 VEE680 pKa = 4.19 RR681 pKa = 11.84 EE682 pKa = 4.0 KK683 pKa = 11.31 LGTDD687 pKa = 3.21 SLHH690 pKa = 6.88 NLGQVDD696 pKa = 4.04 VSDD699 pKa = 4.12 LGEE702 pKa = 4.42 GIKK705 pKa = 10.51 DD706 pKa = 3.61 YY707 pKa = 11.47 GSVLMSQYY715 pKa = 11.2 GKK717 pKa = 8.83 YY718 pKa = 5.92 TTEE721 pKa = 3.3 EE722 pKa = 4.2 RR723 pKa = 11.84 IYY725 pKa = 10.97 KK726 pKa = 10.19 SIVSATGRR734 pKa = 11.84 QLSITRR740 pKa = 11.84 STRR743 pKa = 11.84 LSVVDD748 pKa = 3.69 VRR750 pKa = 11.84 HH751 pKa = 5.42 DD752 pKa = 3.34 TKK754 pKa = 11.11 YY755 pKa = 10.12 GYY757 pKa = 10.8 GRR759 pKa = 11.84 ALYY762 pKa = 9.89 GYY764 pKa = 10.07 LRR766 pKa = 11.84 NLVNLPYY773 pKa = 10.08 VEE775 pKa = 4.26 KK776 pKa = 10.83 ARR778 pKa = 11.84 FVGISPLAMLDD789 pKa = 3.47 AKK791 pKa = 11.34 GMGTLRR797 pKa = 11.84 KK798 pKa = 9.39 YY799 pKa = 11.09 IMGASDD805 pKa = 3.03 VDD807 pKa = 3.51 YY808 pKa = 10.28 TLRR811 pKa = 11.84 VLLL814 pKa = 4.58
Molecular weight: 93.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.285
IPC2_protein 8.317
IPC_protein 8.185
Toseland 8.653
ProMoST 8.697
Dawson 9.063
Bjellqvist 8.99
Wikipedia 9.238
Rodwell 9.194
Grimsley 9.063
Solomon 9.121
Lehninger 9.077
Nozaki 9.033
DTASelect 8.887
Thurlkill 8.96
EMBOSS 9.194
Sillero 9.151
Patrickios 4.457
IPC_peptide 9.107
IPC2_peptide 7.673
IPC2.peptide.svr19 7.659
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1583
769
814
791.5
89.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.528 ± 0.613
1.011 ± 0.074
5.559 ± 0.072
5.938 ± 0.35
4.548 ± 0.48
6.822 ± 1.006
2.401 ± 0.33
4.864 ± 0.23
4.611 ± 1.668
7.96 ± 0.267
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.716 ± 0.371
4.422 ± 1.05
4.169 ± 1.235
3.285 ± 0.216
6.128 ± 0.68
6.254 ± 0.582
6.507 ± 0.856
7.833 ± 1.073
1.769 ± 0.324
4.675 ± 0.186
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here