Amino acid dipepetide frequency for Bacillus phage Palmer

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.026AlaAla: 9.026 ± 0.899
0.471AlaCys: 0.471 ± 0.186
5.023AlaAsp: 5.023 ± 0.516
5.808AlaGlu: 5.808 ± 1.183
3.061AlaPhe: 3.061 ± 0.423
6.985AlaGly: 6.985 ± 0.805
0.785AlaHis: 0.785 ± 0.286
4.081AlaIle: 4.081 ± 0.469
5.416AlaLys: 5.416 ± 0.903
5.808AlaLeu: 5.808 ± 0.827
2.669AlaMet: 2.669 ± 0.326
3.924AlaAsn: 3.924 ± 0.571
3.375AlaPro: 3.375 ± 0.715
3.375AlaGln: 3.375 ± 0.797
4.238AlaArg: 4.238 ± 0.535
3.846AlaSer: 3.846 ± 0.62
4.866AlaThr: 4.866 ± 0.57
4.16AlaVal: 4.16 ± 0.51
0.863AlaTrp: 0.863 ± 0.227
2.826AlaTyr: 2.826 ± 0.439
0.0AlaXaa: 0.0 ± 0.0
Cys
0.471CysAla: 0.471 ± 0.186
0.157CysCys: 0.157 ± 0.117
0.471CysAsp: 0.471 ± 0.203
0.942CysGlu: 0.942 ± 0.34
0.157CysPhe: 0.157 ± 0.116
0.628CysGly: 0.628 ± 0.192
0.157CysHis: 0.157 ± 0.126
0.235CysIle: 0.235 ± 0.143
0.471CysLys: 0.471 ± 0.189
0.235CysLeu: 0.235 ± 0.147
0.314CysMet: 0.314 ± 0.186
0.471CysAsn: 0.471 ± 0.233
0.314CysPro: 0.314 ± 0.152
0.235CysGln: 0.235 ± 0.142
0.314CysArg: 0.314 ± 0.134
0.392CysSer: 0.392 ± 0.194
0.392CysThr: 0.392 ± 0.155
0.314CysVal: 0.314 ± 0.183
0.078CysTrp: 0.078 ± 0.067
0.235CysTyr: 0.235 ± 0.114
0.0CysXaa: 0.0 ± 0.0
Asp
3.61AspAla: 3.61 ± 0.432
0.549AspCys: 0.549 ± 0.245
2.826AspAsp: 2.826 ± 0.483
4.081AspGlu: 4.081 ± 0.553
3.061AspPhe: 3.061 ± 0.369
4.238AspGly: 4.238 ± 0.638
0.392AspHis: 0.392 ± 0.185
4.709AspIle: 4.709 ± 0.681
4.16AspLys: 4.16 ± 0.56
4.395AspLeu: 4.395 ± 0.541
1.57AspMet: 1.57 ± 0.49
3.375AspAsn: 3.375 ± 0.471
1.491AspPro: 1.491 ± 0.266
2.041AspGln: 2.041 ± 0.435
1.884AspArg: 1.884 ± 0.376
2.826AspSer: 2.826 ± 0.446
2.669AspThr: 2.669 ± 0.495
4.238AspVal: 4.238 ± 0.626
0.942AspTrp: 0.942 ± 0.275
2.433AspTyr: 2.433 ± 0.442
0.0AspXaa: 0.0 ± 0.0
Glu
6.2GluAla: 6.2 ± 0.836
0.628GluCys: 0.628 ± 0.236
2.826GluAsp: 2.826 ± 0.484
4.945GluGlu: 4.945 ± 1.004
2.512GluPhe: 2.512 ± 0.382
4.16GluGly: 4.16 ± 0.563
1.57GluHis: 1.57 ± 0.331
5.416GluIle: 5.416 ± 0.798
6.2GluLys: 6.2 ± 0.659
5.887GluLeu: 5.887 ± 1.02
2.904GluMet: 2.904 ± 0.572
4.317GluAsn: 4.317 ± 0.635
2.119GluPro: 2.119 ± 0.422
4.317GluGln: 4.317 ± 0.949
3.296GluArg: 3.296 ± 0.482
2.826GluSer: 2.826 ± 0.473
2.982GluThr: 2.982 ± 0.573
4.552GluVal: 4.552 ± 0.682
0.942GluTrp: 0.942 ± 0.29
2.982GluTyr: 2.982 ± 0.449
0.0GluXaa: 0.0 ± 0.0
Phe
1.805PheAla: 1.805 ± 0.345
0.392PheCys: 0.392 ± 0.186
2.826PheAsp: 2.826 ± 0.442
3.139PheGlu: 3.139 ± 0.578
1.648PhePhe: 1.648 ± 0.321
2.982PheGly: 2.982 ± 0.332
0.628PheHis: 0.628 ± 0.225
3.453PheIle: 3.453 ± 0.637
3.139PheLys: 3.139 ± 0.523
2.982PheLeu: 2.982 ± 0.551
1.256PheMet: 1.256 ± 0.293
3.139PheAsn: 3.139 ± 0.583
1.727PhePro: 1.727 ± 0.266
1.884PheGln: 1.884 ± 0.39
1.727PheArg: 1.727 ± 0.446
2.982PheSer: 2.982 ± 0.457
2.826PheThr: 2.826 ± 0.495
1.413PheVal: 1.413 ± 0.272
0.314PheTrp: 0.314 ± 0.137
1.099PheTyr: 1.099 ± 0.299
0.0PheXaa: 0.0 ± 0.0
Gly
6.279GlyAla: 6.279 ± 0.734
0.157GlyCys: 0.157 ± 0.106
2.904GlyAsp: 2.904 ± 0.4
4.16GlyGlu: 4.16 ± 0.698
2.119GlyPhe: 2.119 ± 0.408
5.887GlyGly: 5.887 ± 0.907
0.785GlyHis: 0.785 ± 0.195
3.767GlyIle: 3.767 ± 0.455
6.043GlyLys: 6.043 ± 1.075
5.18GlyLeu: 5.18 ± 0.613
2.276GlyMet: 2.276 ± 0.412
3.767GlyAsn: 3.767 ± 0.474
1.256GlyPro: 1.256 ± 0.361
2.904GlyGln: 2.904 ± 0.639
2.826GlyArg: 2.826 ± 0.467
3.689GlySer: 3.689 ± 0.556
5.494GlyThr: 5.494 ± 1.006
5.808GlyVal: 5.808 ± 0.738
1.02GlyTrp: 1.02 ± 0.29
2.747GlyTyr: 2.747 ± 0.366
0.0GlyXaa: 0.0 ± 0.0
His
0.942HisAla: 0.942 ± 0.274
0.235HisCys: 0.235 ± 0.148
1.648HisAsp: 1.648 ± 0.356
0.785HisGlu: 0.785 ± 0.306
0.942HisPhe: 0.942 ± 0.237
0.549HisGly: 0.549 ± 0.18
0.235HisHis: 0.235 ± 0.144
1.02HisIle: 1.02 ± 0.255
1.413HisLys: 1.413 ± 0.249
1.02HisLeu: 1.02 ± 0.32
0.314HisMet: 0.314 ± 0.184
1.334HisAsn: 1.334 ± 0.327
0.942HisPro: 0.942 ± 0.273
0.628HisGln: 0.628 ± 0.184
0.628HisArg: 0.628 ± 0.202
0.785HisSer: 0.785 ± 0.206
0.785HisThr: 0.785 ± 0.296
0.549HisVal: 0.549 ± 0.185
0.314HisTrp: 0.314 ± 0.172
0.942HisTyr: 0.942 ± 0.261
0.0HisXaa: 0.0 ± 0.0
Ile
4.945IleAla: 4.945 ± 0.783
0.471IleCys: 0.471 ± 0.179
4.631IleAsp: 4.631 ± 0.816
4.631IleGlu: 4.631 ± 0.724
1.962IlePhe: 1.962 ± 0.448
3.532IleGly: 3.532 ± 0.545
1.491IleHis: 1.491 ± 0.379
4.16IleIle: 4.16 ± 0.707
5.887IleLys: 5.887 ± 0.745
3.767IleLeu: 3.767 ± 0.506
1.256IleMet: 1.256 ± 0.342
4.552IleAsn: 4.552 ± 0.594
1.962IlePro: 1.962 ± 0.24
3.061IleGln: 3.061 ± 0.484
2.041IleArg: 2.041 ± 0.488
2.747IleSer: 2.747 ± 0.529
3.846IleThr: 3.846 ± 0.622
3.375IleVal: 3.375 ± 0.53
0.549IleTrp: 0.549 ± 0.189
2.276IleTyr: 2.276 ± 0.43
0.0IleXaa: 0.0 ± 0.0
Lys
6.828LysAla: 6.828 ± 0.894
0.785LysCys: 0.785 ± 0.233
4.16LysAsp: 4.16 ± 0.558
6.907LysGlu: 6.907 ± 0.755
2.826LysPhe: 2.826 ± 0.426
5.651LysGly: 5.651 ± 0.829
1.256LysHis: 1.256 ± 0.298
4.317LysIle: 4.317 ± 0.7
7.142LysLys: 7.142 ± 0.871
5.573LysLeu: 5.573 ± 0.685
2.433LysMet: 2.433 ± 0.536
5.887LysAsn: 5.887 ± 0.539
2.826LysPro: 2.826 ± 0.517
5.023LysGln: 5.023 ± 0.973
3.846LysArg: 3.846 ± 0.576
4.474LysSer: 4.474 ± 0.67
4.866LysThr: 4.866 ± 0.655
4.474LysVal: 4.474 ± 0.548
0.549LysTrp: 0.549 ± 0.211
2.982LysTyr: 2.982 ± 0.547
0.0LysXaa: 0.0 ± 0.0
Leu
4.866LeuAla: 4.866 ± 0.542
0.314LeuCys: 0.314 ± 0.143
3.139LeuAsp: 3.139 ± 0.43
6.593LeuGlu: 6.593 ± 0.949
2.669LeuPhe: 2.669 ± 0.389
4.866LeuGly: 4.866 ± 0.637
1.256LeuHis: 1.256 ± 0.26
4.003LeuIle: 4.003 ± 0.786
6.436LeuLys: 6.436 ± 0.823
5.102LeuLeu: 5.102 ± 0.865
1.884LeuMet: 1.884 ± 0.398
5.573LeuAsn: 5.573 ± 0.643
1.491LeuPro: 1.491 ± 0.381
2.512LeuGln: 2.512 ± 0.459
2.982LeuArg: 2.982 ± 0.613
5.18LeuSer: 5.18 ± 0.521
5.965LeuThr: 5.965 ± 0.748
3.61LeuVal: 3.61 ± 0.466
1.177LeuTrp: 1.177 ± 0.322
2.119LeuTyr: 2.119 ± 0.458
0.0LeuXaa: 0.0 ± 0.0
Met
2.041MetAla: 2.041 ± 0.362
0.235MetCys: 0.235 ± 0.129
1.884MetAsp: 1.884 ± 0.393
1.256MetGlu: 1.256 ± 0.28
1.57MetPhe: 1.57 ± 0.362
1.727MetGly: 1.727 ± 0.344
0.392MetHis: 0.392 ± 0.231
1.57MetIle: 1.57 ± 0.393
2.747MetLys: 2.747 ± 0.451
1.491MetLeu: 1.491 ± 0.453
0.628MetMet: 0.628 ± 0.252
1.648MetAsn: 1.648 ± 0.406
1.962MetPro: 1.962 ± 0.415
1.805MetGln: 1.805 ± 0.419
1.256MetArg: 1.256 ± 0.349
1.648MetSer: 1.648 ± 0.356
1.805MetThr: 1.805 ± 0.532
1.648MetVal: 1.648 ± 0.374
0.078MetTrp: 0.078 ± 0.073
0.942MetTyr: 0.942 ± 0.293
0.0MetXaa: 0.0 ± 0.0
Asn
6.2AsnAla: 6.2 ± 0.766
0.471AsnCys: 0.471 ± 0.213
3.532AsnAsp: 3.532 ± 0.513
3.218AsnGlu: 3.218 ± 0.555
2.512AsnPhe: 2.512 ± 0.489
4.395AsnGly: 4.395 ± 0.515
1.256AsnHis: 1.256 ± 0.299
3.218AsnIle: 3.218 ± 0.538
4.631AsnLys: 4.631 ± 0.683
3.689AsnLeu: 3.689 ± 0.617
1.962AsnMet: 1.962 ± 0.335
3.375AsnAsn: 3.375 ± 0.569
2.982AsnPro: 2.982 ± 0.917
2.59AsnGln: 2.59 ± 0.485
3.139AsnArg: 3.139 ± 0.508
3.218AsnSer: 3.218 ± 0.43
2.982AsnThr: 2.982 ± 0.5
5.023AsnVal: 5.023 ± 0.708
0.863AsnTrp: 0.863 ± 0.253
2.433AsnTyr: 2.433 ± 0.466
0.0AsnXaa: 0.0 ± 0.0
Pro
3.532ProAla: 3.532 ± 0.546
0.235ProCys: 0.235 ± 0.14
2.041ProAsp: 2.041 ± 0.427
3.296ProGlu: 3.296 ± 0.485
1.727ProPhe: 1.727 ± 0.433
2.355ProGly: 2.355 ± 0.413
0.314ProHis: 0.314 ± 0.164
2.669ProIle: 2.669 ± 0.523
2.512ProLys: 2.512 ± 0.491
2.826ProLeu: 2.826 ± 0.565
0.549ProMet: 0.549 ± 0.224
1.727ProAsn: 1.727 ± 0.458
1.648ProPro: 1.648 ± 0.793
1.884ProGln: 1.884 ± 0.454
0.863ProArg: 0.863 ± 0.218
1.962ProSer: 1.962 ± 0.291
2.512ProThr: 2.512 ± 0.618
2.198ProVal: 2.198 ± 0.394
0.471ProTrp: 0.471 ± 0.21
1.727ProTyr: 1.727 ± 0.537
0.0ProXaa: 0.0 ± 0.0
Gln
4.474GlnAla: 4.474 ± 0.689
0.078GlnCys: 0.078 ± 0.081
2.355GlnAsp: 2.355 ± 0.551
4.16GlnGlu: 4.16 ± 0.821
2.433GlnPhe: 2.433 ± 0.693
3.296GlnGly: 3.296 ± 0.616
0.471GlnHis: 0.471 ± 0.179
1.727GlnIle: 1.727 ± 0.322
3.689GlnLys: 3.689 ± 0.795
4.945GlnLeu: 4.945 ± 0.602
1.256GlnMet: 1.256 ± 0.347
2.904GlnAsn: 2.904 ± 0.769
1.57GlnPro: 1.57 ± 0.363
4.395GlnGln: 4.395 ± 1.504
2.119GlnArg: 2.119 ± 0.487
2.198GlnSer: 2.198 ± 0.371
1.884GlnThr: 1.884 ± 0.284
2.119GlnVal: 2.119 ± 0.311
1.099GlnTrp: 1.099 ± 0.564
1.648GlnTyr: 1.648 ± 0.466
0.0GlnXaa: 0.0 ± 0.0
Arg
3.61ArgAla: 3.61 ± 0.572
0.628ArgCys: 0.628 ± 0.206
2.276ArgAsp: 2.276 ± 0.443
3.453ArgGlu: 3.453 ± 0.487
2.041ArgPhe: 2.041 ± 0.443
2.355ArgGly: 2.355 ± 0.497
0.785ArgHis: 0.785 ± 0.229
2.276ArgIle: 2.276 ± 0.352
3.296ArgLys: 3.296 ± 0.383
3.924ArgLeu: 3.924 ± 0.533
1.177ArgMet: 1.177 ± 0.231
2.198ArgAsn: 2.198 ± 0.45
0.706ArgPro: 0.706 ± 0.228
1.884ArgGln: 1.884 ± 0.446
1.648ArgArg: 1.648 ± 0.379
2.59ArgSer: 2.59 ± 0.466
1.884ArgThr: 1.884 ± 0.405
2.355ArgVal: 2.355 ± 0.355
0.314ArgTrp: 0.314 ± 0.146
1.884ArgTyr: 1.884 ± 0.357
0.0ArgXaa: 0.0 ± 0.0
Ser
3.375SerAla: 3.375 ± 0.396
0.078SerCys: 0.078 ± 0.074
3.296SerAsp: 3.296 ± 0.559
2.747SerGlu: 2.747 ± 0.478
3.453SerPhe: 3.453 ± 0.587
4.631SerGly: 4.631 ± 0.63
1.099SerHis: 1.099 ± 0.315
3.296SerIle: 3.296 ± 0.532
4.866SerLys: 4.866 ± 0.516
4.238SerLeu: 4.238 ± 0.608
1.727SerMet: 1.727 ± 0.323
2.904SerAsn: 2.904 ± 0.527
2.041SerPro: 2.041 ± 0.394
2.982SerGln: 2.982 ± 0.688
1.413SerArg: 1.413 ± 0.417
4.238SerSer: 4.238 ± 0.629
3.218SerThr: 3.218 ± 0.634
2.826SerVal: 2.826 ± 0.39
0.706SerTrp: 0.706 ± 0.216
2.59SerTyr: 2.59 ± 0.53
0.0SerXaa: 0.0 ± 0.0
Thr
5.416ThrAla: 5.416 ± 0.793
0.314ThrCys: 0.314 ± 0.169
2.433ThrAsp: 2.433 ± 0.509
3.924ThrGlu: 3.924 ± 0.498
2.355ThrPhe: 2.355 ± 0.399
3.689ThrGly: 3.689 ± 0.549
1.02ThrHis: 1.02 ± 0.36
4.631ThrIle: 4.631 ± 0.871
4.317ThrLys: 4.317 ± 0.471
3.296ThrLeu: 3.296 ± 0.402
0.942ThrMet: 0.942 ± 0.266
3.846ThrAsn: 3.846 ± 0.61
3.924ThrPro: 3.924 ± 0.493
2.433ThrGln: 2.433 ± 0.356
1.727ThrArg: 1.727 ± 0.3
3.924ThrSer: 3.924 ± 0.721
3.767ThrThr: 3.767 ± 0.852
4.317ThrVal: 4.317 ± 0.685
0.706ThrTrp: 0.706 ± 0.247
2.747ThrTyr: 2.747 ± 0.501
0.0ThrXaa: 0.0 ± 0.0
Val
4.317ValAla: 4.317 ± 0.444
0.392ValCys: 0.392 ± 0.264
4.238ValAsp: 4.238 ± 0.546
4.16ValGlu: 4.16 ± 0.654
2.669ValPhe: 2.669 ± 0.48
4.16ValGly: 4.16 ± 0.572
1.256ValHis: 1.256 ± 0.208
3.375ValIle: 3.375 ± 0.494
4.945ValLys: 4.945 ± 0.533
3.846ValLeu: 3.846 ± 0.557
1.884ValMet: 1.884 ± 0.416
3.375ValAsn: 3.375 ± 0.654
2.826ValPro: 2.826 ± 0.377
2.433ValGln: 2.433 ± 0.465
2.512ValArg: 2.512 ± 0.417
3.689ValSer: 3.689 ± 0.496
4.238ValThr: 4.238 ± 0.51
2.904ValVal: 2.904 ± 0.547
0.314ValTrp: 0.314 ± 0.145
2.041ValTyr: 2.041 ± 0.603
0.0ValXaa: 0.0 ± 0.0
Trp
0.706TrpAla: 0.706 ± 0.229
0.078TrpCys: 0.078 ± 0.074
0.392TrpAsp: 0.392 ± 0.134
0.706TrpGlu: 0.706 ± 0.254
0.314TrpPhe: 0.314 ± 0.148
0.549TrpGly: 0.549 ± 0.21
0.157TrpHis: 0.157 ± 0.125
0.706TrpIle: 0.706 ± 0.216
1.884TrpLys: 1.884 ± 0.391
1.02TrpLeu: 1.02 ± 0.278
0.235TrpMet: 0.235 ± 0.144
1.02TrpAsn: 1.02 ± 0.337
0.235TrpPro: 0.235 ± 0.174
1.02TrpGln: 1.02 ± 0.508
0.628TrpArg: 0.628 ± 0.264
0.549TrpSer: 0.549 ± 0.223
0.706TrpThr: 0.706 ± 0.287
0.863TrpVal: 0.863 ± 0.267
0.078TrpTrp: 0.078 ± 0.07
0.549TrpTyr: 0.549 ± 0.167
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.962TyrAla: 1.962 ± 0.328
0.314TyrCys: 0.314 ± 0.175
2.669TyrAsp: 2.669 ± 0.43
2.669TyrGlu: 2.669 ± 0.483
1.334TyrPhe: 1.334 ± 0.388
2.433TyrGly: 2.433 ± 0.457
0.706TyrHis: 0.706 ± 0.235
2.512TyrIle: 2.512 ± 0.744
3.61TyrLys: 3.61 ± 0.675
2.512TyrLeu: 2.512 ± 0.447
1.02TyrMet: 1.02 ± 0.311
2.355TyrAsn: 2.355 ± 0.377
1.648TyrPro: 1.648 ± 0.418
1.413TyrGln: 1.413 ± 0.363
2.198TyrArg: 2.198 ± 0.357
1.962TyrSer: 1.962 ± 0.31
2.041TyrThr: 2.041 ± 0.297
2.826TyrVal: 2.826 ± 0.67
1.02TyrTrp: 1.02 ± 0.248
1.413TyrTyr: 1.413 ± 0.348
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 50 proteins (12742 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski