Actinomycetales bacterium JB111

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Actinomycetales; unclassified Actinomycetales

Average proteome isoelectric point is 5.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2923 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1R4F4C1|A0A1R4F4C1_9ACTO 30S ribosomal protein S4 OS=Actinomycetales bacterium JB111 OX=1434822 GN=rpsD PE=3 SV=1
MM1 pKa = 7.63AGADD5 pKa = 4.26RR6 pKa = 11.84LPLPDD11 pKa = 5.86GIATTLLVQGTSISRR26 pKa = 11.84IQGRR30 pKa = 11.84LEE32 pKa = 3.67QTLLSDD38 pKa = 3.97GSASQRR44 pKa = 11.84LRR46 pKa = 11.84GAFPAGLSAEE56 pKa = 4.77AIDD59 pKa = 4.32TEE61 pKa = 4.48LSVLSGHH68 pKa = 6.42VSTATSNMGSAGQHH82 pKa = 4.56VWDD85 pKa = 4.32VGAALEE91 pKa = 4.39TCEE94 pKa = 5.04SEE96 pKa = 5.17VEE98 pKa = 4.01GLQADD103 pKa = 4.09WDD105 pKa = 4.1EE106 pKa = 4.33LDD108 pKa = 3.25AWFDD112 pKa = 3.59RR113 pKa = 11.84ALADD117 pKa = 3.81INSDD121 pKa = 3.29PYY123 pKa = 10.77YY124 pKa = 10.86AEE126 pKa = 4.98PEE128 pKa = 4.18NQRR131 pKa = 11.84DD132 pKa = 3.26RR133 pKa = 11.84AEE135 pKa = 4.59AIEE138 pKa = 5.06SIDD141 pKa = 3.65LQRR144 pKa = 11.84EE145 pKa = 4.03SGRR148 pKa = 11.84DD149 pKa = 3.54DD150 pKa = 3.74LKK152 pKa = 11.16EE153 pKa = 4.22RR154 pKa = 11.84YY155 pKa = 8.96HH156 pKa = 6.99SAIDD160 pKa = 3.68TFGDD164 pKa = 3.57AASAAATRR172 pKa = 11.84LTALTDD178 pKa = 4.72GIVPPSQQGSVTDD191 pKa = 3.73IAAAMTAGTTIADD204 pKa = 3.87HH205 pKa = 7.57DD206 pKa = 4.42RR207 pKa = 11.84ATHH210 pKa = 4.65VAEE213 pKa = 4.58EE214 pKa = 4.07QAEE217 pKa = 4.23DD218 pKa = 4.25ASDD221 pKa = 3.64LLEE224 pKa = 4.42RR225 pKa = 11.84ADD227 pKa = 5.15DD228 pKa = 3.93EE229 pKa = 4.63SLSAEE234 pKa = 4.15EE235 pKa = 3.83RR236 pKa = 11.84QEE238 pKa = 5.4AIDD241 pKa = 4.46EE242 pKa = 4.42FNATYY247 pKa = 11.02GDD249 pKa = 4.24LADD252 pKa = 5.08DD253 pKa = 5.06PYY255 pKa = 11.43FSAALMDD262 pKa = 4.77EE263 pKa = 4.28IGEE266 pKa = 4.26EE267 pKa = 4.0EE268 pKa = 4.58LMQHH272 pKa = 6.63IANLQDD278 pKa = 3.64YY279 pKa = 10.43QGEE282 pKa = 4.34NVEE285 pKa = 4.39NEE287 pKa = 4.35SVDD290 pKa = 3.85ASIDD294 pKa = 3.37TYY296 pKa = 11.76LNVLGSAFVTSTNDD310 pKa = 3.3PVGLSDD316 pKa = 3.87YY317 pKa = 9.87ATEE320 pKa = 4.33RR321 pKa = 11.84LTPAAEE327 pKa = 3.96TRR329 pKa = 11.84RR330 pKa = 11.84DD331 pKa = 3.74EE332 pKa = 4.22FTAAADD338 pKa = 3.65EE339 pKa = 4.52YY340 pKa = 11.52YY341 pKa = 10.06PYY343 pKa = 10.85DD344 pKa = 4.69DD345 pKa = 5.34GNGHH349 pKa = 5.96VPGWWSIGQLINGAQFADD367 pKa = 3.98VPVTPDD373 pKa = 2.95RR374 pKa = 11.84AFTEE378 pKa = 4.3SLGADD383 pKa = 3.3MMRR386 pKa = 11.84FYY388 pKa = 11.16YY389 pKa = 10.73DD390 pKa = 3.51PDD392 pKa = 3.99TVVGMQTNNPMNMSPTGNPYY412 pKa = 10.36PYY414 pKa = 10.18DD415 pKa = 3.58SLVEE419 pKa = 4.29FPEE422 pKa = 4.52GLEE425 pKa = 4.1LPGGVDD431 pKa = 3.52PEE433 pKa = 4.39QMFRR437 pKa = 11.84DD438 pKa = 3.84PLYY441 pKa = 10.38SLNYY445 pKa = 9.94SVTGDD450 pKa = 3.44EE451 pKa = 4.16EE452 pKa = 4.52AATGLYY458 pKa = 10.06LSEE461 pKa = 4.55VGTHH465 pKa = 5.46EE466 pKa = 4.35VNGEE470 pKa = 4.01QVPVSGMEE478 pKa = 4.12YY479 pKa = 10.39LMHH482 pKa = 6.93RR483 pKa = 11.84AVNYY487 pKa = 9.22EE488 pKa = 3.97PAYY491 pKa = 10.09HH492 pKa = 6.74EE493 pKa = 5.23AMLSPVVEE501 pKa = 4.6GAHH504 pKa = 5.42NVARR508 pKa = 11.84DD509 pKa = 3.76PYY511 pKa = 10.53DD512 pKa = 3.61EE513 pKa = 5.51EE514 pKa = 4.54STQVAQEE521 pKa = 3.94FVDD524 pKa = 3.21AHH526 pKa = 6.26LRR528 pKa = 11.84LIGGEE533 pKa = 4.01YY534 pKa = 10.14EE535 pKa = 4.38NYY537 pKa = 10.35VDD539 pKa = 3.81TTFMTTSVADD549 pKa = 3.29VWAANIDD556 pKa = 3.8SLMYY560 pKa = 10.76AVDD563 pKa = 4.22DD564 pKa = 4.9DD565 pKa = 4.45GVAVGEE571 pKa = 4.34DD572 pKa = 3.32GVYY575 pKa = 10.61AGNGPTQADD584 pKa = 3.99YY585 pKa = 10.38MFSISDD591 pKa = 3.59DD592 pKa = 3.33VRR594 pKa = 11.84AYY596 pKa = 10.98LPDD599 pKa = 3.36LMNYY603 pKa = 9.2FAEE606 pKa = 4.42DD607 pKa = 3.51RR608 pKa = 11.84PGEE611 pKa = 4.06FVIDD615 pKa = 4.18PNGPDD620 pKa = 3.58AGNPPALQRR629 pKa = 11.84IIDD632 pKa = 3.78ATVLDD637 pKa = 4.19SHH639 pKa = 7.47FDD641 pKa = 3.3AQNIFDD647 pKa = 4.35PEE649 pKa = 4.63HH650 pKa = 5.65YY651 pKa = 9.94QSLSGAEE658 pKa = 3.9NALQVSTDD666 pKa = 3.44QSSLFLNYY674 pKa = 9.97VNEE677 pKa = 4.28SLDD680 pKa = 3.54QQGAADD686 pKa = 4.43DD687 pKa = 4.35AQLEE691 pKa = 4.25QTRR694 pKa = 11.84GYY696 pKa = 10.56INGAVSGASGLIAGLGTTGRR716 pKa = 11.84FVYY719 pKa = 10.4GMADD723 pKa = 4.1DD724 pKa = 4.97LVMDD728 pKa = 5.14AFWDD732 pKa = 4.04AVGEE736 pKa = 4.23NYY738 pKa = 10.76DD739 pKa = 3.88PANASNSAVNFNSALEE755 pKa = 4.08DD756 pKa = 3.16AVYY759 pKa = 10.4QGAYY763 pKa = 9.46SAEE766 pKa = 4.17PWSVDD771 pKa = 2.67PWDD774 pKa = 4.22AQTNPAGVMSPEE786 pKa = 3.93QWSEE790 pKa = 3.59ATGQPRR796 pKa = 11.84FDD798 pKa = 4.1TDD800 pKa = 4.51NPADD804 pKa = 3.4WTPNQRR810 pKa = 11.84RR811 pKa = 11.84AYY813 pKa = 8.24EE814 pKa = 4.38AYY816 pKa = 10.26AIDD819 pKa = 4.33PNGGMGNYY827 pKa = 9.0TSGPVTEE834 pKa = 5.21IGDD837 pKa = 3.88SATEE841 pKa = 4.01AEE843 pKa = 4.59QLVTAHH849 pKa = 7.69RR850 pKa = 11.84SQADD854 pKa = 3.22QQ855 pKa = 3.31

Molecular weight: 92.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1R4F9K3|A0A1R4F9K3_9ACTO Ribosomal RNA small subunit methyltransferase G OS=Actinomycetales bacterium JB111 OX=1434822 GN=rsmG PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.45VHH17 pKa = 5.31GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84TKK38 pKa = 10.64GRR40 pKa = 11.84AKK42 pKa = 10.67LSAA45 pKa = 3.92

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2923

0

2923

961640

37

2177

329.0

34.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.554 ± 0.076

0.504 ± 0.01

6.759 ± 0.046

6.038 ± 0.045

2.587 ± 0.027

9.715 ± 0.047

2.034 ± 0.021

3.966 ± 0.033

1.49 ± 0.031

9.767 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.826 ± 0.02

1.721 ± 0.024

5.721 ± 0.039

2.494 ± 0.023

7.531 ± 0.05

5.673 ± 0.032

6.241 ± 0.036

9.198 ± 0.046

1.382 ± 0.019

1.798 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski