Amino acid dipepetide frequency for Actinomycetales bacterium JB111

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.336AlaAla: 20.336 ± 0.216	0.771AlaCys: 0.771 ± 0.027	9.517AlaAsp: 9.517 ± 0.109	8.851AlaGlu: 8.851 ± 0.125	3.204AlaPhe: 3.204 ± 0.061	13.823AlaGly: 13.823 ± 0.144	2.563AlaHis: 2.563 ± 0.055	5.301AlaIle: 5.301 ± 0.093	2.023AlaLys: 2.023 ± 0.063	13.43AlaLeu: 13.43 ± 0.132	2.79AlaMet: 2.79 ± 0.054	2.016AlaAsn: 2.016 ± 0.051	7.105AlaPro: 7.105 ± 0.103	3.315AlaGln: 3.315 ± 0.062	10.094AlaArg: 10.094 ± 0.152	6.867AlaSer: 6.867 ± 0.088	7.856AlaThr: 7.856 ± 0.092	11.634AlaVal: 11.634 ± 0.127	1.834AlaTrp: 1.834 ± 0.05	2.211AlaTyr: 2.211 ± 0.05	0.0AlaXaa: 0.0 ± 0.0
Cys	0.662CysAla: 0.662 ± 0.028	0.055CysCys: 0.055 ± 0.007	0.298CysAsp: 0.298 ± 0.02	0.251CysGlu: 0.251 ± 0.016	0.139CysPhe: 0.139 ± 0.013	0.687CysGly: 0.687 ± 0.032	0.122CysHis: 0.122 ± 0.011	0.151CysIle: 0.151 ± 0.015	0.045CysLys: 0.045 ± 0.007	0.46CysLeu: 0.46 ± 0.024	0.081CysMet: 0.081 ± 0.009	0.088CysAsn: 0.088 ± 0.01	0.312CysPro: 0.312 ± 0.021	0.113CysGln: 0.113 ± 0.011	0.343CysArg: 0.343 ± 0.02	0.305CysSer: 0.305 ± 0.019	0.343CysThr: 0.343 ± 0.021	0.433CysVal: 0.433 ± 0.02	0.054CysTrp: 0.054 ± 0.008	0.099CysTyr: 0.099 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	9.052AspAla: 9.052 ± 0.099	0.249AspCys: 0.249 ± 0.018	5.21AspAsp: 5.21 ± 0.088	4.779AspGlu: 4.779 ± 0.081	1.488AspPhe: 1.488 ± 0.04	7.201AspGly: 7.201 ± 0.104	1.424AspHis: 1.424 ± 0.04	2.183AspIle: 2.183 ± 0.049	0.758AspLys: 0.758 ± 0.032	7.164AspLeu: 7.164 ± 0.089	0.985AspMet: 0.985 ± 0.028	0.88AspAsn: 0.88 ± 0.037	4.772AspPro: 4.772 ± 0.084	1.696AspGln: 1.696 ± 0.047	4.796AspArg: 4.796 ± 0.069	3.108AspSer: 3.108 ± 0.064	3.165AspThr: 3.165 ± 0.06	6.602AspVal: 6.602 ± 0.083	0.949AspTrp: 0.949 ± 0.037	1.13AspTyr: 1.13 ± 0.037	0.0AspXaa: 0.0 ± 0.0
Glu	7.735GluAla: 7.735 ± 0.111	0.268GluCys: 0.268 ± 0.02	3.738GluAsp: 3.738 ± 0.069	3.708GluGlu: 3.708 ± 0.075	1.514GluPhe: 1.514 ± 0.044	4.368GluGly: 4.368 ± 0.063	1.581GluHis: 1.581 ± 0.045	3.108GluIle: 3.108 ± 0.061	1.181GluLys: 1.181 ± 0.04	6.341GluLeu: 6.341 ± 0.093	1.135GluMet: 1.135 ± 0.037	1.249GluAsn: 1.249 ± 0.047	3.409GluPro: 3.409 ± 0.064	2.189GluGln: 2.189 ± 0.06	5.301GluArg: 5.301 ± 0.079	3.337GluSer: 3.337 ± 0.062	3.128GluThr: 3.128 ± 0.063	5.215GluVal: 5.215 ± 0.071	0.785GluTrp: 0.785 ± 0.026	1.089GluTyr: 1.089 ± 0.034	0.0GluXaa: 0.0 ± 0.0
Phe	3.505PheAla: 3.505 ± 0.062	0.156PheCys: 0.156 ± 0.013	1.992PheAsp: 1.992 ± 0.049	1.439PheGlu: 1.439 ± 0.045	0.809PhePhe: 0.809 ± 0.029	2.962PheGly: 2.962 ± 0.059	0.5PheHis: 0.5 ± 0.022	0.843PheIle: 0.843 ± 0.037	0.305PheLys: 0.305 ± 0.019	2.357PheLeu: 2.357 ± 0.057	0.41PheMet: 0.41 ± 0.024	0.493PheAsn: 0.493 ± 0.024	1.192PhePro: 1.192 ± 0.033	0.591PheGln: 0.591 ± 0.024	1.512PheArg: 1.512 ± 0.04	1.632PheSer: 1.632 ± 0.042	1.744PheThr: 1.744 ± 0.045	2.556PheVal: 2.556 ± 0.059	0.357PheTrp: 0.357 ± 0.018	0.515PheTyr: 0.515 ± 0.028	0.0PheXaa: 0.0 ± 0.0
Gly	12.122GlyAla: 12.122 ± 0.116	0.544GlyCys: 0.544 ± 0.025	5.864GlyAsp: 5.864 ± 0.11	5.989GlyGlu: 5.989 ± 0.082	2.677GlyPhe: 2.677 ± 0.061	9.198GlyGly: 9.198 ± 0.129	1.95GlyHis: 1.95 ± 0.055	4.337GlyIle: 4.337 ± 0.074	1.702GlyLys: 1.702 ± 0.049	9.159GlyLeu: 9.159 ± 0.117	2.058GlyMet: 2.058 ± 0.044	1.72GlyAsn: 1.72 ± 0.048	5.11GlyPro: 5.11 ± 0.095	2.641GlyGln: 2.641 ± 0.068	7.795GlyArg: 7.795 ± 0.117	6.284GlySer: 6.284 ± 0.093	6.29GlyThr: 6.29 ± 0.092	8.132GlyVal: 8.132 ± 0.105	1.57GlyTrp: 1.57 ± 0.044	2.007GlyTyr: 2.007 ± 0.051	0.0GlyXaa: 0.0 ± 0.0
His	2.621HisAla: 2.621 ± 0.06	0.128HisCys: 0.128 ± 0.012	1.404HisAsp: 1.404 ± 0.035	1.201HisGlu: 1.201 ± 0.039	0.417HisPhe: 0.417 ± 0.021	2.035HisGly: 2.035 ± 0.046	0.605HisHis: 0.605 ± 0.027	0.621HisIle: 0.621 ± 0.024	0.221HisLys: 0.221 ± 0.016	2.299HisLeu: 2.299 ± 0.053	0.306HisMet: 0.306 ± 0.017	0.317HisAsn: 0.317 ± 0.019	1.528HisPro: 1.528 ± 0.041	0.475HisGln: 0.475 ± 0.024	1.676HisArg: 1.676 ± 0.039	1.0HisSer: 1.0 ± 0.032	0.992HisThr: 0.992 ± 0.034	1.876HisVal: 1.876 ± 0.044	0.246HisTrp: 0.246 ± 0.015	0.374HisTyr: 0.374 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	5.998IleAla: 5.998 ± 0.081	0.224IleCys: 0.224 ± 0.015	3.373IleAsp: 3.373 ± 0.06	2.602IleGlu: 2.602 ± 0.05	0.931IlePhe: 0.931 ± 0.032	4.328IleGly: 4.328 ± 0.076	0.664IleHis: 0.664 ± 0.028	1.379IleIle: 1.379 ± 0.043	0.549IleLys: 0.549 ± 0.023	3.37IleLeu: 3.37 ± 0.068	0.574IleMet: 0.574 ± 0.024	0.752IleAsn: 0.752 ± 0.03	2.082IlePro: 2.082 ± 0.049	0.782IleGln: 0.782 ± 0.027	2.371IleArg: 2.371 ± 0.047	2.124IleSer: 2.124 ± 0.052	2.391IleThr: 2.391 ± 0.048	4.083IleVal: 4.083 ± 0.071	0.426IleTrp: 0.426 ± 0.022	0.66IleTyr: 0.66 ± 0.025	0.0IleXaa: 0.0 ± 0.0
Lys	1.825LysAla: 1.825 ± 0.056	0.048LysCys: 0.048 ± 0.008	1.05LysAsp: 1.05 ± 0.037	0.921LysGlu: 0.921 ± 0.037	0.342LysPhe: 0.342 ± 0.02	1.189LysGly: 1.189 ± 0.041	0.356LysHis: 0.356 ± 0.018	0.74LysIle: 0.74 ± 0.035	0.576LysLys: 0.576 ± 0.031	1.225LysLeu: 1.225 ± 0.042	0.311LysMet: 0.311 ± 0.016	0.38LysAsn: 0.38 ± 0.023	0.796LysPro: 0.796 ± 0.035	0.467LysGln: 0.467 ± 0.024	1.116LysArg: 1.116 ± 0.041	0.876LysSer: 0.876 ± 0.029	0.812LysThr: 0.812 ± 0.035	1.405LysVal: 1.405 ± 0.047	0.157LysTrp: 0.157 ± 0.013	0.311LysTyr: 0.311 ± 0.017	0.0LysXaa: 0.0 ± 0.0
Leu	15.331LeuAla: 15.331 ± 0.16	0.475LeuCys: 0.475 ± 0.025	6.592LeuAsp: 6.592 ± 0.089	5.141LeuGlu: 5.141 ± 0.076	2.311LeuPhe: 2.311 ± 0.054	9.625LeuGly: 9.625 ± 0.099	1.766LeuHis: 1.766 ± 0.044	3.317LeuIle: 3.317 ± 0.069	1.375LeuLys: 1.375 ± 0.052	9.656LeuLeu: 9.656 ± 0.139	1.644LeuMet: 1.644 ± 0.039	1.415LeuAsn: 1.415 ± 0.038	5.55LeuPro: 5.55 ± 0.081	1.995LeuGln: 1.995 ± 0.047	7.188LeuArg: 7.188 ± 0.093	5.203LeuSer: 5.203 ± 0.075	6.796LeuThr: 6.796 ± 0.088	9.816LeuVal: 9.816 ± 0.109	1.095LeuTrp: 1.095 ± 0.036	1.377LeuTyr: 1.377 ± 0.036	0.0LeuXaa: 0.0 ± 0.0
Met	2.183MetAla: 2.183 ± 0.046	0.103MetCys: 0.103 ± 0.01	0.912MetAsp: 0.912 ± 0.03	0.751MetGlu: 0.751 ± 0.026	0.552MetPhe: 0.552 ± 0.023	1.394MetGly: 1.394 ± 0.038	0.362MetHis: 0.362 ± 0.022	0.766MetIle: 0.766 ± 0.028	0.292MetLys: 0.292 ± 0.017	1.797MetLeu: 1.797 ± 0.042	0.313MetMet: 0.313 ± 0.021	0.434MetAsn: 0.434 ± 0.02	1.203MetPro: 1.203 ± 0.036	0.48MetGln: 0.48 ± 0.025	1.448MetArg: 1.448 ± 0.038	1.527MetSer: 1.527 ± 0.04	1.712MetThr: 1.712 ± 0.04	1.464MetVal: 1.464 ± 0.032	0.22MetTrp: 0.22 ± 0.015	0.345MetTyr: 0.345 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	2.003AsnAla: 2.003 ± 0.042	0.09AsnCys: 0.09 ± 0.01	1.05AsnAsp: 1.05 ± 0.036	0.93AsnGlu: 0.93 ± 0.033	0.519AsnPhe: 0.519 ± 0.025	1.696AsnGly: 1.696 ± 0.05	0.415AsnHis: 0.415 ± 0.022	0.726AsnIle: 0.726 ± 0.028	0.261AsnLys: 0.261 ± 0.018	1.782AsnLeu: 1.782 ± 0.041	0.308AsnMet: 0.308 ± 0.018	0.396AsnAsn: 0.396 ± 0.023	1.404AsnPro: 1.404 ± 0.037	0.506AsnGln: 0.506 ± 0.021	1.175AsnArg: 1.175 ± 0.032	0.907AsnSer: 0.907 ± 0.033	0.889AsnThr: 0.889 ± 0.035	1.482AsnVal: 1.482 ± 0.038	0.244AsnTrp: 0.244 ± 0.017	0.428AsnTyr: 0.428 ± 0.025	0.0AsnXaa: 0.0 ± 0.0
Pro	7.928ProAla: 7.928 ± 0.107	0.21ProCys: 0.21 ± 0.017	4.841ProAsp: 4.841 ± 0.083	4.181ProGlu: 4.181 ± 0.075	1.488ProPhe: 1.488 ± 0.035	6.312ProGly: 6.312 ± 0.101	1.166ProHis: 1.166 ± 0.038	1.889ProIle: 1.889 ± 0.052	0.82ProLys: 0.82 ± 0.036	4.498ProLeu: 4.498 ± 0.074	0.957ProMet: 0.957 ± 0.03	0.944ProAsn: 0.944 ± 0.029	2.708ProPro: 2.708 ± 0.067	1.293ProGln: 1.293 ± 0.04	3.671ProArg: 3.671 ± 0.073	3.425ProSer: 3.425 ± 0.059	3.965ProThr: 3.965 ± 0.073	5.033ProVal: 5.033 ± 0.085	0.834ProTrp: 0.834 ± 0.03	1.048ProTyr: 1.048 ± 0.035	0.0ProXaa: 0.0 ± 0.0
Gln	3.308GlnAla: 3.308 ± 0.056	0.122GlnCys: 0.122 ± 0.011	1.332GlnAsp: 1.332 ± 0.042	1.315GlnGlu: 1.315 ± 0.038	0.741GlnPhe: 0.741 ± 0.027	2.081GlnGly: 2.081 ± 0.054	0.537GlnHis: 0.537 ± 0.021	1.312GlnIle: 1.312 ± 0.038	0.389GlnLys: 0.389 ± 0.022	2.428GlnLeu: 2.428 ± 0.054	0.521GlnMet: 0.521 ± 0.026	0.503GlnAsn: 0.503 ± 0.024	1.259GlnPro: 1.259 ± 0.04	0.961GlnGln: 0.961 ± 0.048	2.008GlnArg: 2.008 ± 0.05	1.335GlnSer: 1.335 ± 0.036	1.293GlnThr: 1.293 ± 0.037	2.613GlnVal: 2.613 ± 0.054	0.397GlnTrp: 0.397 ± 0.019	0.489GlnTyr: 0.489 ± 0.023	0.0GlnXaa: 0.0 ± 0.0
Arg	9.943ArgAla: 9.943 ± 0.122	0.316ArgCys: 0.316 ± 0.02	4.144ArgAsp: 4.144 ± 0.076	4.636ArgGlu: 4.636 ± 0.08	1.983ArgPhe: 1.983 ± 0.044	5.897ArgGly: 5.897 ± 0.101	1.646ArgHis: 1.646 ± 0.043	3.385ArgIle: 3.385 ± 0.063	1.153ArgLys: 1.153 ± 0.038	7.438ArgLeu: 7.438 ± 0.106	1.661ArgMet: 1.661 ± 0.043	1.149ArgAsn: 1.149 ± 0.036	4.536ArgPro: 4.536 ± 0.084	1.914ArgGln: 1.914 ± 0.056	7.516ArgArg: 7.516 ± 0.122	4.598ArgSer: 4.598 ± 0.089	4.917ArgThr: 4.917 ± 0.072	5.868ArgVal: 5.868 ± 0.081	1.192ArgTrp: 1.192 ± 0.035	1.414ArgTyr: 1.414 ± 0.04	0.0ArgXaa: 0.0 ± 0.0
Ser	7.347SerAla: 7.347 ± 0.104	0.271SerCys: 0.271 ± 0.018	3.645SerAsp: 3.645 ± 0.077	2.957SerGlu: 2.957 ± 0.063	1.614SerPhe: 1.614 ± 0.038	6.569SerGly: 6.569 ± 0.098	1.027SerHis: 1.027 ± 0.037	2.259SerIle: 2.259 ± 0.05	0.787SerLys: 0.787 ± 0.034	5.248SerLeu: 5.248 ± 0.069	1.177SerMet: 1.177 ± 0.034	1.015SerAsn: 1.015 ± 0.037	3.399SerPro: 3.399 ± 0.07	1.281SerGln: 1.281 ± 0.039	3.953SerArg: 3.953 ± 0.073	3.718SerSer: 3.718 ± 0.083	3.796SerThr: 3.796 ± 0.065	4.706SerVal: 4.706 ± 0.067	0.85SerTrp: 0.85 ± 0.034	1.114SerTyr: 1.114 ± 0.038	0.0SerXaa: 0.0 ± 0.0
Thr	7.626ThrAla: 7.626 ± 0.09	0.307ThrCys: 0.307 ± 0.019	4.221ThrAsp: 4.221 ± 0.077	3.529ThrGlu: 3.529 ± 0.066	1.886ThrPhe: 1.886 ± 0.049	6.515ThrGly: 6.515 ± 0.09	1.218ThrHis: 1.218 ± 0.035	2.735ThrIle: 2.735 ± 0.053	0.889ThrLys: 0.889 ± 0.038	5.63ThrLeu: 5.63 ± 0.084	1.101ThrMet: 1.101 ± 0.031	1.226ThrAsn: 1.226 ± 0.037	3.985ThrPro: 3.985 ± 0.069	1.327ThrGln: 1.327 ± 0.036	3.88ThrArg: 3.88 ± 0.069	3.869ThrSer: 3.869 ± 0.07	4.479ThrThr: 4.479 ± 0.084	5.712ThrVal: 5.712 ± 0.093	0.941ThrTrp: 0.941 ± 0.033	1.336ThrTyr: 1.336 ± 0.037	0.0ThrXaa: 0.0 ± 0.0
Val	12.307ValAla: 12.307 ± 0.125	0.501ValCys: 0.501 ± 0.025	6.343ValAsp: 6.343 ± 0.079	5.463ValGlu: 5.463 ± 0.09	2.181ValPhe: 2.181 ± 0.057	8.348ValGly: 8.348 ± 0.105	1.821ValHis: 1.821 ± 0.047	3.468ValIle: 3.468 ± 0.064	1.131ValLys: 1.131 ± 0.041	9.907ValLeu: 9.907 ± 0.136	1.389ValMet: 1.389 ± 0.039	1.56ValAsn: 1.56 ± 0.045	5.193ValPro: 5.193 ± 0.072	1.96ValGln: 1.96 ± 0.041	6.745ValArg: 6.745 ± 0.1	4.706ValSer: 4.706 ± 0.072	5.981ValThr: 5.981 ± 0.087	10.555ValVal: 10.555 ± 0.13	1.064ValTrp: 1.064 ± 0.034	1.352ValTyr: 1.352 ± 0.037	0.0ValXaa: 0.0 ± 0.0
Trp	1.53TrpAla: 1.53 ± 0.045	0.092TrpCys: 0.092 ± 0.01	0.891TrpAsp: 0.891 ± 0.038	0.672TrpGlu: 0.672 ± 0.028	0.475TrpPhe: 0.475 ± 0.024	1.0TrpGly: 1.0 ± 0.032	0.301TrpHis: 0.301 ± 0.018	0.607TrpIle: 0.607 ± 0.028	0.197TrpLys: 0.197 ± 0.015	1.515TrpLeu: 1.515 ± 0.048	0.266TrpMet: 0.266 ± 0.016	0.337TrpAsn: 0.337 ± 0.023	0.698TrpPro: 0.698 ± 0.03	0.485TrpGln: 0.485 ± 0.024	1.266TrpArg: 1.266 ± 0.037	0.907TrpSer: 0.907 ± 0.028	0.851TrpThr: 0.851 ± 0.031	1.096TrpVal: 1.096 ± 0.034	0.343TrpTrp: 0.343 ± 0.018	0.289TrpTyr: 0.289 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.181TyrAla: 2.181 ± 0.048	0.112TyrCys: 0.112 ± 0.013	1.173TyrAsp: 1.173 ± 0.038	1.063TyrGlu: 1.063 ± 0.035	0.598TyrPhe: 0.598 ± 0.028	1.921TyrGly: 1.921 ± 0.055	0.321TyrHis: 0.321 ± 0.018	0.535TyrIle: 0.535 ± 0.025	0.246TyrLys: 0.246 ± 0.014	1.96TyrLeu: 1.96 ± 0.043	0.271TyrMet: 0.271 ± 0.016	0.338TyrAsn: 0.338 ± 0.019	0.972TyrPro: 0.972 ± 0.031	0.473TyrGln: 0.473 ± 0.021	1.456TyrArg: 1.456 ± 0.042	1.005TyrSer: 1.005 ± 0.036	1.013TyrThr: 1.013 ± 0.032	1.695TyrVal: 1.695 ± 0.051	0.256TyrTrp: 0.256 ± 0.016	0.391TyrTyr: 0.391 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2923 proteins (961640 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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