Amylibacter cionae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Amylibacter

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3948 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N5ILU8|A0A2N5ILU8_9RHOB Guanine deaminase OS=Amylibacter cionae OX=2035344 GN=C0U40_02015 PE=4 SV=1
MM1 pKa = 6.95VRR3 pKa = 11.84STTFEE8 pKa = 4.44FICMPMITHH17 pKa = 6.53TNDD20 pKa = 3.23RR21 pKa = 11.84ARR23 pKa = 11.84KK24 pKa = 8.55AAITPRR30 pKa = 11.84AACMRR35 pKa = 11.84SSAHH39 pKa = 5.68CFARR43 pKa = 11.84LFVRR47 pKa = 11.84YY48 pKa = 9.24SVLLLLAVLFALVKK62 pKa = 10.76SGGVWAQQLILNPTGAPTVVGNGIGKK88 pKa = 9.78RR89 pKa = 11.84GVWRR93 pKa = 11.84NVGTVNGASVDD104 pKa = 3.21IVGIMTLATRR114 pKa = 11.84DD115 pKa = 3.52HH116 pKa = 7.08RR117 pKa = 11.84FGTGNGQIMITSVGQDD133 pKa = 2.89PHH135 pKa = 7.15FADD138 pKa = 3.93FHH140 pKa = 8.08IYY142 pKa = 9.64EE143 pKa = 4.77AGTYY147 pKa = 10.43NIFSNSGGVPVVADD161 pKa = 3.46VDD163 pKa = 3.77IQINDD168 pKa = 2.88IDD170 pKa = 4.54GPVNEE175 pKa = 4.08QVYY178 pKa = 11.16VNLCDD183 pKa = 4.03GSVEE187 pKa = 4.1YY188 pKa = 11.21VRR190 pKa = 11.84VDD192 pKa = 3.76RR193 pKa = 11.84NATTYY198 pKa = 10.15RR199 pKa = 11.84GYY201 pKa = 10.32IEE203 pKa = 5.93GPDD206 pKa = 3.51ANLGTEE212 pKa = 4.15VFYY215 pKa = 11.03LAGDD219 pKa = 3.45QNYY222 pKa = 10.42ANQPVSGLEE231 pKa = 3.3ISYY234 pKa = 9.06PQTSTFSFGRR244 pKa = 11.84TANNGFLVLLANPTYY259 pKa = 10.47DD260 pKa = 3.79VSDD263 pKa = 3.83TYY265 pKa = 10.89EE266 pKa = 4.18LKK268 pKa = 11.09CGDD271 pKa = 3.82FKK273 pKa = 11.78APVLQDD279 pKa = 4.14DD280 pKa = 4.18IKK282 pKa = 10.62EE283 pKa = 4.19QVLGEE288 pKa = 4.11PVTVNILFNDD298 pKa = 3.86SVATEE303 pKa = 4.22NDD305 pKa = 2.95NAPANNSQQPSEE317 pKa = 4.23YY318 pKa = 10.95AMQAIDD324 pKa = 5.75LIPPTGALNIVTDD337 pKa = 4.12SAGHH341 pKa = 5.77RR342 pKa = 11.84VGFDD346 pKa = 3.35VIGEE350 pKa = 4.49GTWSYY355 pKa = 12.03DD356 pKa = 3.37DD357 pKa = 3.55VTGEE361 pKa = 3.95LTFTPFVAFFAAPTPIDD378 pKa = 3.53YY379 pKa = 10.5RR380 pKa = 11.84FQSPIILPSEE390 pKa = 4.04PQAYY394 pKa = 8.9SAPARR399 pKa = 11.84VSIDD403 pKa = 3.36VGSVGLLKK411 pKa = 10.54LAQLVDD417 pKa = 3.88TNLNGYY423 pKa = 9.4ADD425 pKa = 4.32PGEE428 pKa = 4.36TIAYY432 pKa = 9.14VFTAEE437 pKa = 4.1NFGNVDD443 pKa = 3.57LTNVQLAEE451 pKa = 4.29TQFSGAGIPPVITFQAATSLSPEE474 pKa = 3.84GTLLVGEE481 pKa = 4.4KK482 pKa = 10.26AVYY485 pKa = 7.91TATYY489 pKa = 9.11TLVPEE494 pKa = 5.2DD495 pKa = 4.76LDD497 pKa = 4.03TTISNQAEE505 pKa = 4.34VTAQTPSGTTVSDD518 pKa = 3.93LSDD521 pKa = 3.67SEE523 pKa = 4.42NPSDD527 pKa = 3.88GDD529 pKa = 3.71GVARR533 pKa = 11.84NGPGEE538 pKa = 4.19GRR540 pKa = 11.84DD541 pKa = 3.91DD542 pKa = 3.61PTTIYY547 pKa = 10.61AGSGPDD553 pKa = 4.01RR554 pKa = 11.84GDD556 pKa = 3.35APITYY561 pKa = 10.05GDD563 pKa = 4.06PQHH566 pKa = 7.14ADD568 pKa = 3.04TSAYY572 pKa = 9.16WIGALNGDD580 pKa = 3.97GDD582 pKa = 4.12GSAQHH587 pKa = 6.48SADD590 pKa = 3.42GTGDD594 pKa = 3.87DD595 pKa = 5.81LDD597 pKa = 4.75GKK599 pKa = 10.5DD600 pKa = 5.28DD601 pKa = 3.83EE602 pKa = 5.08SEE604 pKa = 4.26EE605 pKa = 4.39NFPQLYY611 pKa = 10.66GDD613 pKa = 4.16LTRR616 pKa = 11.84TVTIPVNEE624 pKa = 4.04PVPGSGYY631 pKa = 10.14LQAFIDD637 pKa = 3.98FGGDD641 pKa = 3.21GTFLSLGDD649 pKa = 3.82QVATDD654 pKa = 3.73IQDD657 pKa = 3.95GGPQDD662 pKa = 4.92LDD664 pKa = 3.34GTANGEE670 pKa = 3.64ISFAISVPATAVLTPTFARR689 pKa = 11.84LRR691 pKa = 11.84WSSVAGLTAISPATDD706 pKa = 3.7GEE708 pKa = 4.46VEE710 pKa = 4.76DD711 pKa = 4.26YY712 pKa = 11.18GITIKK717 pKa = 10.01TPPEE721 pKa = 4.08ADD723 pKa = 3.59RR724 pKa = 11.84GDD726 pKa = 3.83APASYY731 pKa = 10.79GDD733 pKa = 3.62PQHH736 pKa = 7.29IVEE739 pKa = 5.2GPAAPEE745 pKa = 4.09IYY747 pKa = 10.21LGTIAPDD754 pKa = 3.09VDD756 pKa = 4.85VIAQNTATASGDD768 pKa = 3.82DD769 pKa = 3.97LDD771 pKa = 6.05GNDD774 pKa = 4.88DD775 pKa = 3.57EE776 pKa = 6.91DD777 pKa = 4.91GVVLPTLYY785 pKa = 10.86LGGLAEE791 pKa = 4.29ITVTVNDD798 pKa = 4.0VGSPPQKK805 pKa = 10.04SAYY808 pKa = 9.12LQAFIDD814 pKa = 3.88FDD816 pKa = 4.63GDD818 pKa = 3.84GTFLQAGEE826 pKa = 4.18QVATNLQDD834 pKa = 4.65GDD836 pKa = 4.33PLDD839 pKa = 4.53KK840 pKa = 11.24DD841 pKa = 3.94GASDD845 pKa = 3.54GSIRR849 pKa = 11.84FEE851 pKa = 4.14VAVPAGATTLPTFARR866 pKa = 11.84FRR868 pKa = 11.84WSTDD872 pKa = 2.48ATGQAIAFNGEE883 pKa = 3.93VEE885 pKa = 5.09DD886 pKa = 4.21YY887 pKa = 10.38PLTISGDD894 pKa = 3.96PPPFLCDD901 pKa = 3.45GSIYY905 pKa = 10.24RR906 pKa = 11.84YY907 pKa = 10.6DD908 pKa = 3.71DD909 pKa = 3.48DD910 pKa = 4.36RR911 pKa = 11.84VVRR914 pKa = 11.84RR915 pKa = 11.84LTFTASGSSYY925 pKa = 11.14AIGFQDD931 pKa = 4.02IGTAPRR937 pKa = 11.84NLNANWGYY945 pKa = 10.52NALDD949 pKa = 3.84GYY951 pKa = 9.97MYY953 pKa = 10.85AVRR956 pKa = 11.84PGRR959 pKa = 11.84SDD961 pKa = 4.29LYY963 pKa = 11.11RR964 pKa = 11.84LDD966 pKa = 3.68GAGNFVDD973 pKa = 4.74LTNLPGGTADD983 pKa = 3.76GSGAGDD989 pKa = 3.57IMPNGTMVYY998 pKa = 9.68VANGNTWQLISLVDD1012 pKa = 3.6ASNPVNLGVLNLSQSVDD1029 pKa = 3.4TRR1031 pKa = 11.84DD1032 pKa = 3.12IAYY1035 pKa = 10.35NPVDD1039 pKa = 3.33GLFYY1043 pKa = 10.83GINQSSGRR1051 pKa = 11.84AFKK1054 pKa = 10.68VDD1056 pKa = 3.69PNGGVAGLATVTEE1069 pKa = 5.06FGPAIYY1075 pKa = 10.12GGIFGSVWFDD1085 pKa = 3.02QDD1087 pKa = 2.69GRR1089 pKa = 11.84FYY1091 pKa = 11.22VYY1093 pKa = 11.24SNTTNNLYY1101 pKa = 8.83LTNTTTGEE1109 pKa = 3.9AQLIANSTVDD1119 pKa = 3.39EE1120 pKa = 5.09AGDD1123 pKa = 3.86SDD1125 pKa = 4.32GMSCRR1130 pKa = 11.84GPAPIPFGAISGNVYY1145 pKa = 10.99DD1146 pKa = 5.01DD1147 pKa = 4.3TNASDD1152 pKa = 3.81VKK1154 pKa = 11.49DD1155 pKa = 3.59NGEE1158 pKa = 4.23TNLGAGIAISVYY1170 pKa = 10.74ADD1172 pKa = 2.98SGTPADD1178 pKa = 3.9TSDD1181 pKa = 4.29DD1182 pKa = 3.91LFLITTDD1189 pKa = 3.44TEE1191 pKa = 4.34ADD1193 pKa = 3.15GTYY1196 pKa = 10.89AVADD1200 pKa = 3.83LLINTNYY1207 pKa = 10.0RR1208 pKa = 11.84VEE1210 pKa = 4.54LDD1212 pKa = 3.74EE1213 pKa = 5.96ADD1215 pKa = 4.01PDD1217 pKa = 3.87LGGGKK1222 pKa = 8.45TIGTSNPRR1230 pKa = 11.84LGVEE1234 pKa = 4.14VTAGEE1239 pKa = 4.43TTTDD1243 pKa = 2.84QDD1245 pKa = 3.92FGFDD1249 pKa = 3.3AAASDD1254 pKa = 4.98LEE1256 pKa = 4.32LTKK1259 pKa = 10.95YY1260 pKa = 10.49AALTGTTTPVTNVSEE1275 pKa = 4.32GDD1277 pKa = 3.7VIDD1280 pKa = 4.41WIITITNVSGGSPSGVKK1297 pKa = 10.5VIDD1300 pKa = 5.25LIPSGFAYY1308 pKa = 10.72VSDD1311 pKa = 4.3SAPSTGDD1318 pKa = 2.92TYY1320 pKa = 11.76DD1321 pKa = 4.56PDD1323 pKa = 3.71TGLWFVDD1330 pKa = 4.89EE1331 pKa = 4.45ILAGASEE1338 pKa = 4.4TLVITTTVLDD1348 pKa = 3.88SGEE1351 pKa = 4.17LTNTAEE1357 pKa = 4.8IIYY1360 pKa = 10.41SSLPDD1365 pKa = 4.36PDD1367 pKa = 4.99SDD1369 pKa = 4.19PSTGPLTDD1377 pKa = 4.97DD1378 pKa = 5.15LFDD1381 pKa = 4.82QIADD1385 pKa = 4.04DD1386 pKa = 5.38DD1387 pKa = 4.35EE1388 pKa = 6.53ASYY1391 pKa = 9.74TVNLITNDD1399 pKa = 3.24RR1400 pKa = 11.84VFSGRR1405 pKa = 11.84IFSDD1409 pKa = 2.97NGEE1412 pKa = 4.46SGGTAHH1418 pKa = 7.65DD1419 pKa = 3.89AEE1421 pKa = 4.76INGGEE1426 pKa = 4.21VGLSSALLEE1435 pKa = 4.31IVDD1438 pKa = 3.8NTGTVIATPDD1448 pKa = 3.47VASGGTWTYY1457 pKa = 11.42ALPSSYY1463 pKa = 10.15TGEE1466 pKa = 3.97LTIRR1470 pKa = 11.84AIPANGYY1477 pKa = 9.27RR1478 pKa = 11.84AISEE1482 pKa = 4.28ATNALPGLSNPDD1494 pKa = 3.2PHH1496 pKa = 7.43DD1497 pKa = 3.82GSFTFTPDD1505 pKa = 3.17PSGNLVNLDD1514 pKa = 3.27IGLLEE1519 pKa = 4.51LPILTEE1525 pKa = 4.24DD1526 pKa = 3.3RR1527 pKa = 11.84VSTVGQGQIVALPHH1541 pKa = 7.06LYY1543 pKa = 9.2TATSDD1548 pKa = 3.62GSVTFSLADD1557 pKa = 3.43STSAPTGAFSAALYY1571 pKa = 10.46HH1572 pKa = 7.17DD1573 pKa = 5.26ASCTGAPGVPVTGPLSVSAGEE1594 pKa = 4.37TICIQSRR1601 pKa = 11.84ISAGAGAGTGSRR1613 pKa = 11.84FTYY1616 pKa = 10.17KK1617 pKa = 10.33IIAKK1621 pKa = 6.5TTFTGTTAVSSASNTDD1637 pKa = 3.19QVTVTAEE1644 pKa = 3.78SGQVHH1649 pKa = 5.66LTKK1652 pKa = 10.28TVEE1655 pKa = 3.89NEE1657 pKa = 4.02TAGTAEE1663 pKa = 4.29ATSNMGGANDD1673 pKa = 3.39VLLYY1677 pKa = 10.43RR1678 pKa = 11.84IYY1680 pKa = 11.07LEE1682 pKa = 4.45NTDD1685 pKa = 3.83TTPVTNVKK1693 pKa = 10.27VYY1695 pKa = 11.07DD1696 pKa = 3.58RR1697 pKa = 11.84TPPYY1701 pKa = 9.25TALSEE1706 pKa = 4.8PIADD1710 pKa = 3.97PQILSPNLSCNLAKK1724 pKa = 10.36PVTNTANYY1732 pKa = 9.87SGLVEE1737 pKa = 4.38WNCSGNFLPGEE1748 pKa = 4.08VGALQFRR1755 pKa = 11.84VAITPP1760 pKa = 3.69

Molecular weight: 184.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N5IG79|A0A2N5IG79_9RHOB CoA-binding protein OS=Amylibacter cionae OX=2035344 GN=C0U40_14645 PE=4 SV=1
MM1 pKa = 7.17HH2 pKa = 7.11VRR4 pKa = 11.84RR5 pKa = 11.84TMYY8 pKa = 7.2EE9 pKa = 3.47TSMSGRR15 pKa = 11.84HH16 pKa = 5.95FISQRR21 pKa = 11.84YY22 pKa = 8.07LFRR25 pKa = 11.84SHH27 pKa = 6.91ALSFTLQKK35 pKa = 9.63LTPEE39 pKa = 4.15RR40 pKa = 11.84SKK42 pKa = 11.6SLLARR47 pKa = 11.84AACLPVPIEE56 pKa = 4.47TEE58 pKa = 4.09SQLPTVLHH66 pKa = 5.6VVSAIAANVGIPPFKK81 pKa = 10.41TGFCAATIQHH91 pKa = 6.06QGRR94 pKa = 11.84KK95 pKa = 10.01AEE97 pKa = 4.1VRR99 pKa = 11.84CKK101 pKa = 10.38RR102 pKa = 11.84EE103 pKa = 3.15RR104 pKa = 11.84DD105 pKa = 3.59AILVSKK111 pKa = 10.53RR112 pKa = 11.84AFKK115 pKa = 10.66ACYY118 pKa = 8.39PIPTNNVRR126 pKa = 11.84LL127 pKa = 3.82

Molecular weight: 14.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3948

0

3948

1297894

21

5932

328.7

35.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.428 ± 0.051

0.919 ± 0.012

6.058 ± 0.047

5.999 ± 0.038

3.925 ± 0.029

8.587 ± 0.04

1.967 ± 0.022

5.441 ± 0.028

3.796 ± 0.036

9.906 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.811 ± 0.026

3.004 ± 0.024

4.759 ± 0.033

3.292 ± 0.021

5.883 ± 0.045

5.609 ± 0.032

5.633 ± 0.036

7.342 ± 0.035

1.287 ± 0.016

2.353 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski