Fusarium graminearum hypovirus 1
Average proteome isoelectric point is 7.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|L7W5G3|L7W5G3_9VIRU Uncharacterized protein OS=Fusarium graminearum hypovirus 1 OX=1284208 GN=FgHV1-HN10_gp1 PE=4 SV=1
MM1 pKa = 8.32 DD2 pKa = 5.35 DD3 pKa = 4.12 PAWLGDD9 pKa = 5.22 ALLEE13 pKa = 3.87 WDD15 pKa = 4.91 LRR17 pKa = 11.84 HH18 pKa = 6.61 ACIAVNGKK26 pKa = 9.82 RR27 pKa = 11.84 NAVWTSEE34 pKa = 3.84 RR35 pKa = 11.84 RR36 pKa = 11.84 SGKK39 pKa = 9.76 ALKK42 pKa = 10.36 KK43 pKa = 10.23 FLVFDD48 pKa = 5.04 GYY50 pKa = 11.04 DD51 pKa = 3.35 VPKK54 pKa = 10.67 AFSLHH59 pKa = 5.64 HH60 pKa = 6.88 CGTIFEE66 pKa = 4.44 YY67 pKa = 10.45 LYY69 pKa = 10.8 HH70 pKa = 7.53 IDD72 pKa = 3.13 EE73 pKa = 4.8 AFRR76 pKa = 11.84 GRR78 pKa = 11.84 YY79 pKa = 8.76 LDD81 pKa = 4.18 YY82 pKa = 11.15 VAAPPDD88 pKa = 3.36 AKK90 pKa = 10.47 RR91 pKa = 11.84 ISEE94 pKa = 4.36 TPTTEE99 pKa = 4.7 CEE101 pKa = 4.17 GHH103 pKa = 6.26 CWRR106 pKa = 11.84 EE107 pKa = 3.75 LFLSYY112 pKa = 10.15 DD113 pKa = 3.31 TFTFDD118 pKa = 3.43 FTGNKK123 pKa = 9.39 SLSRR127 pKa = 11.84 RR128 pKa = 11.84 QLLQLVAKK136 pKa = 8.93 STLKK140 pKa = 10.73 SEE142 pKa = 4.14 TFGVVRR148 pKa = 11.84 HH149 pKa = 5.49 GTQLHH154 pKa = 4.33 VTEE157 pKa = 5.2 GKK159 pKa = 9.19 IAPMEE164 pKa = 4.86 IIRR167 pKa = 11.84 SMVNKK172 pKa = 9.68 EE173 pKa = 3.75 RR174 pKa = 11.84 LGG176 pKa = 3.59
Molecular weight: 20.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.819
IPC2_protein 6.942
IPC_protein 7.132
Toseland 6.985
ProMoST 7.541
Dawson 7.732
Bjellqvist 7.717
Wikipedia 7.658
Rodwell 7.702
Grimsley 7.073
Solomon 7.834
Lehninger 7.863
Nozaki 8.039
DTASelect 7.863
Thurlkill 7.907
EMBOSS 7.995
Sillero 8.156
Patrickios 4.418
IPC_peptide 7.834
IPC2_peptide 7.351
IPC2.peptide.svr19 7.337
Protein with the highest isoelectric point:
>tr|L7W5G3|L7W5G3_9VIRU Uncharacterized protein OS=Fusarium graminearum hypovirus 1 OX=1284208 GN=FgHV1-HN10_gp1 PE=4 SV=1
MM1 pKa = 7.17 AQSTQFSDD9 pKa = 2.56 ISFPLSVRR17 pKa = 11.84 EE18 pKa = 4.33 WYY20 pKa = 8.14 CTARR24 pKa = 11.84 DD25 pKa = 3.58 QSHH28 pKa = 6.75 AEE30 pKa = 3.79 LSQGHH35 pKa = 7.14 EE36 pKa = 4.28 ITCKK40 pKa = 10.23 CQLHH44 pKa = 5.23 MTPPSVQRR52 pKa = 11.84 AKK54 pKa = 10.45 MSGNPEE60 pKa = 4.12 LLAQTLARR68 pKa = 11.84 APSRR72 pKa = 11.84 GPVFAAAWEE81 pKa = 4.35 VHH83 pKa = 6.27 HH84 pKa = 6.87 SCQASNDD91 pKa = 3.35 TRR93 pKa = 11.84 LLDD96 pKa = 3.91 LRR98 pKa = 11.84 FAEE101 pKa = 4.83 LFVSAQDD108 pKa = 3.48 FKK110 pKa = 11.52 SYY112 pKa = 8.8 KK113 pKa = 7.15 TTAIPKK119 pKa = 9.14 EE120 pKa = 4.13 VAPLALKK127 pKa = 10.62 SNPDD131 pKa = 3.49 VSWFGMDD138 pKa = 3.31 QCWASALRR146 pKa = 11.84 PKK148 pKa = 10.5 LATRR152 pKa = 11.84 AVRR155 pKa = 11.84 VAAYY159 pKa = 8.32 EE160 pKa = 3.86 ILLSDD165 pKa = 3.96 FQRR168 pKa = 11.84 LLEE171 pKa = 4.22 LCGKK175 pKa = 10.47 CEE177 pKa = 4.32 GKK179 pKa = 9.79 PMRR182 pKa = 11.84 LIKK185 pKa = 10.4 VHH187 pKa = 6.3 SGLYY191 pKa = 9.37 HH192 pKa = 7.9 LEE194 pKa = 3.93 VEE196 pKa = 4.44 KK197 pKa = 10.99 DD198 pKa = 3.21 KK199 pKa = 10.72 KK200 pKa = 8.7 TSKK203 pKa = 10.62 SAGAKK208 pKa = 9.99 LYY210 pKa = 10.31 RR211 pKa = 11.84 DD212 pKa = 3.26 VSFAVAGSPGARR224 pKa = 11.84 IGNGFSFLFSKK235 pKa = 11.0 GGFTMGGSEE244 pKa = 4.45 PIKK247 pKa = 9.16 NTRR250 pKa = 11.84 PIPRR254 pKa = 11.84 EE255 pKa = 3.7 TSVCWSVPPPRR266 pKa = 11.84 YY267 pKa = 9.79 LEE269 pKa = 4.22 RR270 pKa = 11.84 APLSPVNALDD280 pKa = 3.39 EE281 pKa = 4.35 RR282 pKa = 11.84 EE283 pKa = 3.78 RR284 pKa = 11.84 RR285 pKa = 11.84 LFYY288 pKa = 9.81 TEE290 pKa = 3.47 KK291 pKa = 10.27 RR292 pKa = 11.84 RR293 pKa = 11.84 MRR295 pKa = 11.84 EE296 pKa = 3.58 EE297 pKa = 3.62 RR298 pKa = 11.84 EE299 pKa = 3.85 QRR301 pKa = 11.84 SKK303 pKa = 11.42 ADD305 pKa = 2.94 KK306 pKa = 9.74 HH307 pKa = 6.63 RR308 pKa = 11.84 RR309 pKa = 11.84 MLVLTEE315 pKa = 4.15 RR316 pKa = 11.84 RR317 pKa = 11.84 HH318 pKa = 5.37 SKK320 pKa = 10.51 AVASLLYY327 pKa = 10.71 KK328 pKa = 9.74 MRR330 pKa = 11.84 QGANINKK337 pKa = 7.42 RR338 pKa = 11.84 TPDD341 pKa = 2.72 AWKK344 pKa = 8.82 TNNRR348 pKa = 11.84 YY349 pKa = 9.2 AVLSAHH355 pKa = 6.31 NEE357 pKa = 3.67 RR358 pKa = 11.84 TRR360 pKa = 11.84 PVVFTNVIPVVSMCPGPRR378 pKa = 11.84 TRR380 pKa = 11.84 KK381 pKa = 8.26 TDD383 pKa = 3.51 DD384 pKa = 3.68 TQVSAAKK391 pKa = 10.05 RR392 pKa = 11.84 KK393 pKa = 7.93 STNIQRR399 pKa = 11.84 RR400 pKa = 11.84 NIEE403 pKa = 3.96 GKK405 pKa = 10.07 KK406 pKa = 9.76 EE407 pKa = 3.66 MRR409 pKa = 11.84 HH410 pKa = 3.65 WAFIRR415 pKa = 11.84 RR416 pKa = 11.84 THH418 pKa = 6.01 IQMFTKK424 pKa = 10.39 KK425 pKa = 9.7 IRR427 pKa = 11.84 LAEE430 pKa = 4.11 MNSLDD435 pKa = 4.56 AFFHH439 pKa = 6.45 SYY441 pKa = 10.55 LYY443 pKa = 10.31 PGMDD447 pKa = 3.65 LNQARR452 pKa = 11.84 ILLRR456 pKa = 11.84 VMRR459 pKa = 11.84 GRR461 pKa = 11.84 VVCPTEE467 pKa = 5.71 GYY469 pKa = 10.35 QLDD472 pKa = 3.84 LTFQNKK478 pKa = 8.97 RR479 pKa = 11.84 LEE481 pKa = 4.87 HH482 pKa = 6.39 ISCVPAPTEE491 pKa = 4.06 GVSINIDD498 pKa = 2.92 GHH500 pKa = 5.7 YY501 pKa = 7.61 FTHH504 pKa = 6.83 LGNRR508 pKa = 11.84 RR509 pKa = 11.84 AEE511 pKa = 3.69 IKK513 pKa = 10.52 YY514 pKa = 10.14 RR515 pKa = 11.84 LSAAAWKK522 pKa = 10.25 ARR524 pKa = 11.84 ALSLLKK530 pKa = 10.29 DD531 pKa = 3.8 HH532 pKa = 6.91 QSAAPEE538 pKa = 3.99 RR539 pKa = 11.84 KK540 pKa = 9.33 GKK542 pKa = 10.25 GMEE545 pKa = 4.22 IIGLAKK551 pKa = 10.0 TSVPFARR558 pKa = 11.84 PNLMRR563 pKa = 11.84 RR564 pKa = 11.84 MINGFRR570 pKa = 11.84 VCAIVGGAYY579 pKa = 9.35 IYY581 pKa = 10.64 SRR583 pKa = 11.84 SGRR586 pKa = 11.84 FEE588 pKa = 3.76 MADD591 pKa = 3.28 PRR593 pKa = 11.84 SPGDD597 pKa = 3.8 TITPMQTKK605 pKa = 10.42 VIDD608 pKa = 4.38 DD609 pKa = 4.08 YY610 pKa = 12.3 GHH612 pKa = 7.05 LKK614 pKa = 10.93 YY615 pKa = 10.99 SFTCKK620 pKa = 10.18 GLAKK624 pKa = 10.37 LWVDD628 pKa = 3.75 LGKK631 pKa = 10.69 RR632 pKa = 11.84 LDD634 pKa = 3.37 WRR636 pKa = 11.84 RR637 pKa = 11.84 VLAHH641 pKa = 6.26 SLGMKK646 pKa = 10.1 YY647 pKa = 10.33 SVAEE651 pKa = 3.72 QYY653 pKa = 7.82 MQQWYY658 pKa = 8.56 HH659 pKa = 6.46 HH660 pKa = 6.79 SGLDD664 pKa = 3.52 IVLDD668 pKa = 4.03 SPDD671 pKa = 3.8 DD672 pKa = 3.5 KK673 pKa = 11.09 PYY675 pKa = 11.06 NNRR678 pKa = 11.84 PGQVVHH684 pKa = 7.06 HH685 pKa = 5.83 YY686 pKa = 10.78 EE687 pKa = 3.96 EE688 pKa = 4.44 VTILEE693 pKa = 4.22 PRR695 pKa = 11.84 IEE697 pKa = 4.29 QPTHH701 pKa = 4.41 SRR703 pKa = 11.84 KK704 pKa = 9.57 RR705 pKa = 11.84 VVFGNTNGSGKK716 pKa = 9.78 NVVMPEE722 pKa = 3.71 RR723 pKa = 11.84 RR724 pKa = 11.84 FGKK727 pKa = 8.03 PTSDD731 pKa = 3.18 VVLKK735 pKa = 10.61 FGDD738 pKa = 3.3 MHH740 pKa = 7.07 VIARR744 pKa = 11.84 NADD747 pKa = 3.68 DD748 pKa = 5.92 ADD750 pKa = 3.87 VEE752 pKa = 4.64 LNPGPGHH759 pKa = 6.43 RR760 pKa = 11.84 PSLILEE766 pKa = 4.45 YY767 pKa = 9.61 ATPTADD773 pKa = 3.34 TPASLWYY780 pKa = 10.48 AKK782 pKa = 10.38 FYY784 pKa = 10.97 DD785 pKa = 4.03 DD786 pKa = 5.6 EE787 pKa = 4.24 EE788 pKa = 4.6 WEE790 pKa = 4.15 IDD792 pKa = 3.49 GSALEE797 pKa = 4.55 SVINTYY803 pKa = 7.78 EE804 pKa = 3.93 PKK806 pKa = 10.34 YY807 pKa = 8.82 VEE809 pKa = 4.19 SMGRR813 pKa = 11.84 SWNISEE819 pKa = 4.57 EE820 pKa = 4.25 SAGPHH825 pKa = 6.09 TYY827 pKa = 8.21 HH828 pKa = 6.68 TAKK831 pKa = 10.53 SPKK834 pKa = 9.8 GDD836 pKa = 3.53 VNSEE840 pKa = 3.96 QVRR843 pKa = 11.84 LARR846 pKa = 11.84 FWRR849 pKa = 11.84 RR850 pKa = 11.84 HH851 pKa = 4.2 KK852 pKa = 10.49 RR853 pKa = 11.84 SRR855 pKa = 11.84 SDD857 pKa = 3.47 NPISPLFEE865 pKa = 6.14 AEE867 pKa = 4.08 AHH869 pKa = 5.5 VDD871 pKa = 3.35 PYY873 pKa = 11.43 GADD876 pKa = 3.37 DD877 pKa = 5.15 HH878 pKa = 7.44 DD879 pKa = 4.35 VEE881 pKa = 5.14 KK882 pKa = 11.22 NPGPATPSSSKK893 pKa = 9.12 EE894 pKa = 3.9 AEE896 pKa = 4.17 RR897 pKa = 11.84 GRR899 pKa = 11.84 PAHH902 pKa = 6.63 KK903 pKa = 10.14 RR904 pKa = 11.84 STSEE908 pKa = 3.9 ASSLTLDD915 pKa = 3.78 TNQLEE920 pKa = 4.41 PKK922 pKa = 9.54 PVRR925 pKa = 11.84 EE926 pKa = 3.91 KK927 pKa = 10.94 SRR929 pKa = 11.84 ARR931 pKa = 11.84 SAIRR935 pKa = 11.84 KK936 pKa = 5.36 FTNAIGFTKK945 pKa = 10.41 SDD947 pKa = 3.77 LEE949 pKa = 4.07 QVRR952 pKa = 11.84 LNFGFDD958 pKa = 3.83 LNNEE962 pKa = 3.95 AVLPFTVSDD971 pKa = 4.37 DD972 pKa = 3.63 DD973 pKa = 4.72 TIATLYY979 pKa = 9.8 TGHH982 pKa = 7.23 LVVGHH987 pKa = 6.64 LPGNKK992 pKa = 8.55 IYY994 pKa = 10.34 HH995 pKa = 5.99 KK996 pKa = 9.99 PWRR999 pKa = 11.84 LPIWDD1004 pKa = 4.29 SLLSIEE1010 pKa = 4.96 DD1011 pKa = 3.52 QNVLDD1016 pKa = 4.74 KK1017 pKa = 11.03 NYY1019 pKa = 10.78 SSGPRR1024 pKa = 11.84 FGQPVVAEE1032 pKa = 4.38 VAQMIHH1038 pKa = 6.3 EE1039 pKa = 4.84 FFQRR1043 pKa = 11.84 SPLLCLGRR1051 pKa = 11.84 HH1052 pKa = 5.24 EE1053 pKa = 4.3 MAALAQHH1060 pKa = 6.65 IKK1062 pKa = 10.74 SEE1064 pKa = 4.34 FSCPVITEE1072 pKa = 4.81 GYY1074 pKa = 10.26 AIEE1077 pKa = 5.93 LILATNEE1084 pKa = 3.75 IVLHH1088 pKa = 4.73 YY1089 pKa = 8.22 TATPTSEE1096 pKa = 3.83 TAGRR1100 pKa = 11.84 LWYY1103 pKa = 9.97 YY1104 pKa = 10.54 VSGRR1108 pKa = 11.84 KK1109 pKa = 8.69 YY1110 pKa = 10.85 LHH1112 pKa = 6.6 GGDD1115 pKa = 5.53 DD1116 pKa = 2.96 IGEE1119 pKa = 4.08 NQRR1122 pKa = 11.84 LGSHH1126 pKa = 7.23 TIAQQFTKK1134 pKa = 11.08 GEE1136 pKa = 4.59 AITDD1140 pKa = 3.76 NVITSIDD1147 pKa = 3.54 PTTFSLATGRR1157 pKa = 11.84 RR1158 pKa = 11.84 LAYY1161 pKa = 10.11 LQSQLDD1167 pKa = 4.12 GVKK1170 pKa = 10.28 HH1171 pKa = 5.84 EE1172 pKa = 4.51 EE1173 pKa = 3.82 QSSFDD1178 pKa = 3.63 EE1179 pKa = 4.3 MQAMAIVYY1187 pKa = 9.2 ILLGIIRR1194 pKa = 11.84 KK1195 pKa = 9.19 HH1196 pKa = 6.22 DD1197 pKa = 3.61 ADD1199 pKa = 3.53 MSTTEE1204 pKa = 3.8 GLANIFTIFKK1214 pKa = 10.49 NVLGCPKK1221 pKa = 10.25 SGAYY1225 pKa = 10.05 AINFTNQMTTITIYY1239 pKa = 8.58 QTHH1242 pKa = 7.32 RR1243 pKa = 11.84 LTEE1246 pKa = 4.18 EE1247 pKa = 3.37 NRR1249 pKa = 11.84 EE1250 pKa = 4.17 RR1251 pKa = 11.84 CWVLVEE1257 pKa = 4.27 GEE1259 pKa = 4.34 PLFHH1263 pKa = 7.6 LGISSSEE1270 pKa = 3.96 EE1271 pKa = 3.76 DD1272 pKa = 3.21 SSEE1275 pKa = 3.94 VLGSFDD1281 pKa = 4.36 RR1282 pKa = 11.84 PLSITNPVLQGRR1294 pKa = 11.84 PSWAQARR1301 pKa = 11.84 FYY1303 pKa = 11.02 ANEE1306 pKa = 4.22 MYY1308 pKa = 10.78 DD1309 pKa = 3.81 SISTLPHH1316 pKa = 5.08 TRR1318 pKa = 11.84 DD1319 pKa = 3.1 MRR1321 pKa = 11.84 VVFTVEE1327 pKa = 3.77 HH1328 pKa = 5.88 FQPARR1333 pKa = 11.84 SAFCKK1338 pKa = 10.11 PIEE1341 pKa = 4.19 EE1342 pKa = 4.13 MSINDD1347 pKa = 4.54 VPWMLYY1353 pKa = 8.69 PWLWCIEE1360 pKa = 3.85 HH1361 pKa = 6.03 STRR1364 pKa = 11.84 FWLTLFHH1371 pKa = 7.92 FIRR1374 pKa = 11.84 LWFNMFSIHH1383 pKa = 6.37 RR1384 pKa = 11.84 HH1385 pKa = 5.51 PDD1387 pKa = 3.04 LSGFSPNVTYY1397 pKa = 10.56 PDD1399 pKa = 4.18 DD1400 pKa = 6.49 DD1401 pKa = 4.88 YY1402 pKa = 11.97 DD1403 pKa = 4.69 DD1404 pKa = 5.64 DD1405 pKa = 4.28 YY1406 pKa = 12.02 GRR1408 pKa = 11.84 RR1409 pKa = 11.84 SEE1411 pKa = 4.23 TFAVVTLGTEE1421 pKa = 4.1 GDD1423 pKa = 3.64 NRR1425 pKa = 11.84 PVHH1428 pKa = 6.56 LAAQTAGHH1436 pKa = 6.13 YY1437 pKa = 8.41 GIPTVVRR1444 pKa = 11.84 RR1445 pKa = 11.84 VRR1447 pKa = 11.84 TMDD1450 pKa = 3.06 GHH1452 pKa = 8.22 DD1453 pKa = 3.66 MEE1455 pKa = 4.66 NLRR1458 pKa = 11.84 VGKK1461 pKa = 9.69 VLQYY1465 pKa = 11.13 APDD1468 pKa = 3.67 YY1469 pKa = 10.35 ATIANFATEE1478 pKa = 4.71 GYY1480 pKa = 10.13 KK1481 pKa = 10.38 RR1482 pKa = 11.84 ILAPHH1487 pKa = 6.12 VEE1489 pKa = 3.86 VSMFDD1494 pKa = 3.31 GLSYY1498 pKa = 11.25 SLAPTTHH1505 pKa = 7.53 WIHH1508 pKa = 6.11 TPRR1511 pKa = 11.84 FVDD1514 pKa = 3.47 DD1515 pKa = 3.52 WGKK1518 pKa = 8.55 VTWIDD1523 pKa = 3.78 YY1524 pKa = 10.46 IPAAFMEE1531 pKa = 4.28 QMNSIFSPYY1540 pKa = 10.44 LRR1542 pKa = 11.84 IGCLKK1547 pKa = 10.27 KK1548 pKa = 10.74 GSNFPRR1554 pKa = 11.84 TVDD1557 pKa = 3.24 GFRR1560 pKa = 11.84 PLRR1563 pKa = 11.84 KK1564 pKa = 9.44 KK1565 pKa = 11.07 SNLDD1569 pKa = 3.33 TTGSRR1574 pKa = 11.84 VGWVSGSNNEE1584 pKa = 4.04 HH1585 pKa = 6.68 VIPHH1589 pKa = 7.18 HH1590 pKa = 6.25 IRR1592 pKa = 11.84 EE1593 pKa = 4.26 SCEE1596 pKa = 3.88 RR1597 pKa = 11.84 IPDD1600 pKa = 3.98 GDD1602 pKa = 3.3 HH1603 pKa = 7.1 SEE1605 pKa = 4.13 IFRR1608 pKa = 11.84 HH1609 pKa = 4.69 YY1610 pKa = 10.96 SKK1612 pKa = 10.68 IYY1614 pKa = 7.31 MHH1616 pKa = 6.99 GGAGTVQTAVACGCEE1631 pKa = 4.59 IEE1633 pKa = 4.85 ICDD1636 pKa = 3.55 PTMDD1640 pKa = 4.31 RR1641 pKa = 11.84 NYY1643 pKa = 9.54 HH1644 pKa = 6.03 TIPGPEE1650 pKa = 4.33 DD1651 pKa = 3.14 FHH1653 pKa = 8.33 VPSVSPLMGYY1663 pKa = 10.25 LVLSGFKK1670 pKa = 10.0 PQVPLEE1676 pKa = 4.02 IKK1678 pKa = 10.55 IMWVLSFLWNGKK1690 pKa = 7.59 MWLFLQLIDD1699 pKa = 4.7 YY1700 pKa = 9.38 AIKK1703 pKa = 10.2 CIAMLLSLMTAWKK1716 pKa = 9.27 IALSIYY1722 pKa = 10.34 VSVPLVVLRR1731 pKa = 11.84 LILKK1735 pKa = 7.31 QHH1737 pKa = 5.77 SVSSLLYY1744 pKa = 10.07 LGLWILWEE1752 pKa = 4.59 FPFFCLGQSWGYY1764 pKa = 9.64 FPLVAWTFKK1773 pKa = 10.54 KK1774 pKa = 9.89 SWVRR1778 pKa = 11.84 IVGDD1782 pKa = 3.69 FLAATRR1788 pKa = 11.84 PRR1790 pKa = 11.84 FYY1792 pKa = 10.72 IEE1794 pKa = 4.27 FEE1796 pKa = 3.83 PAKK1799 pKa = 10.27 LEE1801 pKa = 4.26 KK1802 pKa = 10.72 GHH1804 pKa = 6.32 MPFPYY1809 pKa = 10.15 GHH1811 pKa = 7.11 WSVLDD1816 pKa = 3.52 SKK1818 pKa = 10.02 TSYY1821 pKa = 10.15 RR1822 pKa = 11.84 YY1823 pKa = 10.22 EE1824 pKa = 4.03 GMFLKK1829 pKa = 10.59 DD1830 pKa = 3.27 EE1831 pKa = 4.09 RR1832 pKa = 11.84 HH1833 pKa = 5.47 TLGGMFKK1840 pKa = 9.31 FTRR1843 pKa = 11.84 NKK1845 pKa = 10.04 RR1846 pKa = 11.84 VSNRR1850 pKa = 11.84 IKK1852 pKa = 10.74 LSIRR1856 pKa = 11.84 VPFNLDD1862 pKa = 3.07 LAARR1866 pKa = 11.84 RR1867 pKa = 11.84 ALGGKK1872 pKa = 10.03 LIDD1875 pKa = 4.73 AYY1877 pKa = 10.88 SANHH1881 pKa = 6.15 NCLTMVEE1888 pKa = 4.22 QLISTHH1894 pKa = 5.81 SLIGMVALKK1903 pKa = 10.45 LMRR1906 pKa = 11.84 VMIWFTLQPPEE1917 pKa = 3.97 STLRR1921 pKa = 11.84 WMEE1924 pKa = 3.76 VLGYY1928 pKa = 10.97 NIDD1931 pKa = 3.5 NYY1933 pKa = 10.95 RR1934 pKa = 11.84 NSFLYY1939 pKa = 10.71 SKK1941 pKa = 10.65 LGFAASIEE1949 pKa = 4.18 DD1950 pKa = 3.65 VPLEE1954 pKa = 4.27 LEE1956 pKa = 4.54 DD1957 pKa = 5.27 EE1958 pKa = 4.22 ITAPVEE1964 pKa = 3.96 NVPASLDD1971 pKa = 3.74 GEE1973 pKa = 4.31 RR1974 pKa = 11.84 EE1975 pKa = 3.9 TAEE1978 pKa = 4.11 EE1979 pKa = 4.0 IVISEE1984 pKa = 4.56 LDD1986 pKa = 3.48 IEE1988 pKa = 4.82 DD1989 pKa = 3.82 EE1990 pKa = 4.45 SSFEE1994 pKa = 3.92 QVIQEE1999 pKa = 4.34 TVALSAVVSSKK2010 pKa = 11.36 YY2011 pKa = 10.32 EE2012 pKa = 3.64 VDD2014 pKa = 3.07 EE2015 pKa = 4.49 ATTHH2019 pKa = 6.3 EE2020 pKa = 4.13 IVYY2023 pKa = 10.53 DD2024 pKa = 3.74 CVLRR2028 pKa = 11.84 KK2029 pKa = 9.32 AALEE2033 pKa = 4.03 PLPDD2037 pKa = 4.69 DD2038 pKa = 4.19 NVLTRR2043 pKa = 11.84 PRR2045 pKa = 11.84 KK2046 pKa = 9.54 DD2047 pKa = 2.97 GEE2049 pKa = 4.35 AIRR2052 pKa = 11.84 THH2054 pKa = 7.0 LGDD2057 pKa = 3.72 ILDD2060 pKa = 5.48 AIQHH2064 pKa = 6.0 SISFIQHH2071 pKa = 4.28 TRR2073 pKa = 11.84 VGEE2076 pKa = 4.82 AFICWLKK2083 pKa = 11.28 GIGTRR2088 pKa = 11.84 IHH2090 pKa = 6.63 EE2091 pKa = 5.22 FILPLLHH2098 pKa = 6.79 VFTKK2102 pKa = 10.63 VLILGYY2108 pKa = 10.58 VLGEE2112 pKa = 3.75 KK2113 pKa = 10.33 YY2114 pKa = 10.33 FRR2116 pKa = 11.84 NFFIDD2121 pKa = 3.53 VSDD2124 pKa = 5.63 LITYY2128 pKa = 9.98 AYY2130 pKa = 10.22 GLEE2133 pKa = 3.83 KK2134 pKa = 10.4 SKK2136 pKa = 10.64 RR2137 pKa = 11.84 IKK2139 pKa = 8.95 TAWGLTGLYY2148 pKa = 8.2 RR2149 pKa = 11.84 TGFASQKK2156 pKa = 10.45 ARR2158 pKa = 11.84 LALEE2162 pKa = 4.16 IAHH2165 pKa = 6.16 MEE2167 pKa = 4.08 VTEE2170 pKa = 4.53 RR2171 pKa = 11.84 GHH2173 pKa = 7.47 PVDD2176 pKa = 4.15 DD2177 pKa = 4.15 WNNMVAEE2184 pKa = 4.58 INQTAAEE2191 pKa = 4.62 LGVGDD2196 pKa = 4.63 PKK2198 pKa = 10.98 HH2199 pKa = 6.6 VGGPQRR2205 pKa = 11.84 RR2206 pKa = 11.84 PINLKK2211 pKa = 9.79 NQLLTKK2217 pKa = 10.36 RR2218 pKa = 11.84 EE2219 pKa = 4.09 GDD2221 pKa = 3.46 TIMWDD2226 pKa = 2.95 EE2227 pKa = 4.3 SEE2229 pKa = 4.42 YY2230 pKa = 11.12 VRR2232 pKa = 11.84 DD2233 pKa = 3.24 PAYY2236 pKa = 10.2 EE2237 pKa = 3.81 KK2238 pKa = 10.59 RR2239 pKa = 11.84 VEE2241 pKa = 4.02 AKK2243 pKa = 9.83 IATGVPQGSDD2253 pKa = 3.11 QVRR2256 pKa = 11.84 MAKK2259 pKa = 10.0 HH2260 pKa = 5.39 FPEE2263 pKa = 5.56 KK2264 pKa = 9.36 ITEE2267 pKa = 4.45 SIDD2270 pKa = 3.47 RR2271 pKa = 11.84 YY2272 pKa = 8.38 EE2273 pKa = 4.06 PSYY2276 pKa = 11.43 ARR2278 pKa = 11.84 PTSVQIAFAEE2288 pKa = 4.42 EE2289 pKa = 3.94 CADD2292 pKa = 4.83 AEE2294 pKa = 4.76 VEE2296 pKa = 4.09 DD2297 pKa = 4.31 HH2298 pKa = 7.35 PKK2300 pKa = 10.52 FFLNCDD2306 pKa = 2.97 VTMPAAIRR2314 pKa = 11.84 RR2315 pKa = 11.84 YY2316 pKa = 9.51 NKK2318 pKa = 9.47 PKK2320 pKa = 9.75 QKK2322 pKa = 9.96 YY2323 pKa = 8.26 RR2324 pKa = 11.84 PGAFFSGPDD2333 pKa = 3.38 GFRR2336 pKa = 11.84 TRR2338 pKa = 11.84 EE2339 pKa = 3.5 AAARR2343 pKa = 11.84 AGFQKK2348 pKa = 10.79 VADD2351 pKa = 3.96 HH2352 pKa = 6.62 YY2353 pKa = 10.84 IRR2355 pKa = 11.84 KK2356 pKa = 9.26 CFRR2359 pKa = 11.84 EE2360 pKa = 4.32 GKK2362 pKa = 10.08 NILPNYY2368 pKa = 8.53 IAFVKK2373 pKa = 10.39 SQITNAVKK2381 pKa = 10.61 AMPKK2385 pKa = 10.16 SVGGQDD2391 pKa = 2.93 KK2392 pKa = 10.48 QFRR2395 pKa = 11.84 TVVAQDD2401 pKa = 3.7 DD2402 pKa = 3.93 FSYY2405 pKa = 9.79 MQNQVVMMDD2414 pKa = 3.23 RR2415 pKa = 11.84 NKK2417 pKa = 10.52 RR2418 pKa = 11.84 DD2419 pKa = 3.06 WADD2422 pKa = 3.3 EE2423 pKa = 4.37 FGAGAGMRR2431 pKa = 11.84 LNQSMLKK2438 pKa = 10.27 NFLALEE2444 pKa = 4.26 EE2445 pKa = 4.29 PKK2447 pKa = 9.8 EE2448 pKa = 4.35 TFEE2451 pKa = 4.36 GLYY2454 pKa = 10.3 MMADD2458 pKa = 3.38 ATAFDD2463 pKa = 3.94 STIPNIIQCYY2473 pKa = 8.84 HH2474 pKa = 5.61 EE2475 pKa = 4.94 RR2476 pKa = 11.84 LWSHH2480 pKa = 5.97 GFKK2483 pKa = 10.37 NHH2485 pKa = 6.94 PSGNGANIASVAIAATRR2502 pKa = 11.84 AKK2504 pKa = 10.28 SSGWIWGLTEE2514 pKa = 4.76 AEE2516 pKa = 4.12 HH2517 pKa = 6.41 SALKK2521 pKa = 10.55 VVIPEE2526 pKa = 3.62 QGRR2529 pKa = 11.84 RR2530 pKa = 11.84 DD2531 pKa = 3.77 TLVGYY2536 pKa = 10.28 DD2537 pKa = 3.26 SSRR2540 pKa = 11.84 FIDD2543 pKa = 3.68 ATHH2546 pKa = 6.93 LSLAGVTEE2554 pKa = 6.0 LITSGQTLGKK2564 pKa = 9.59 LLLVASRR2571 pKa = 11.84 NQANIPSSVKK2581 pKa = 10.48 DD2582 pKa = 3.52 LGSFIVDD2589 pKa = 3.19 NNANLMSTLRR2599 pKa = 11.84 NKK2601 pKa = 10.11 AHH2603 pKa = 6.43 YY2604 pKa = 9.04 SQTFIYY2610 pKa = 10.45 HH2611 pKa = 6.22 EE2612 pKa = 4.62 GGFNIGTRR2620 pKa = 11.84 QKK2622 pKa = 10.72 GAKK2625 pKa = 8.88 LVPRR2629 pKa = 11.84 AMYY2632 pKa = 10.76 DD2633 pKa = 3.6 DD2634 pKa = 4.18 LAKK2637 pKa = 10.56 FADD2640 pKa = 4.16 APLALLSNMHH2650 pKa = 5.91 YY2651 pKa = 10.51 KK2652 pKa = 10.37 NRR2654 pKa = 11.84 GGDD2657 pKa = 3.17 TGGNEE2662 pKa = 3.84 TTPYY2666 pKa = 9.1 NTLSLRR2672 pKa = 11.84 AIYY2675 pKa = 10.33 RR2676 pKa = 11.84 MAWSLTMNRR2685 pKa = 11.84 PPKK2688 pKa = 10.3 EE2689 pKa = 3.62 FQTYY2693 pKa = 10.43 NKK2695 pKa = 9.44 MSNQGDD2701 pKa = 3.67 DD2702 pKa = 4.38 AMWSSFGKK2710 pKa = 10.69 YY2711 pKa = 10.06 GIRR2714 pKa = 11.84 TYY2716 pKa = 11.33 KK2717 pKa = 10.57 QMLKK2721 pKa = 10.39 FKK2723 pKa = 9.39 EE2724 pKa = 3.87 ICAQMGITMTIDD2736 pKa = 3.08 STKK2739 pKa = 10.79 DD2740 pKa = 3.06 ITKK2743 pKa = 9.95 VEE2745 pKa = 4.17 YY2746 pKa = 10.21 LSKK2749 pKa = 10.25 FVRR2752 pKa = 11.84 RR2753 pKa = 11.84 PTPQDD2758 pKa = 3.61 SEE2760 pKa = 4.82 DD2761 pKa = 3.52 LAVWRR2766 pKa = 11.84 RR2767 pKa = 11.84 HH2768 pKa = 6.25 KK2769 pKa = 10.84 INEE2772 pKa = 4.04 ACNLARR2778 pKa = 11.84 LAGKK2782 pKa = 9.87 DD2783 pKa = 3.59 PNTLDD2788 pKa = 4.22 FSVLNNPKK2796 pKa = 9.74 YY2797 pKa = 10.52 VVYY2800 pKa = 10.62 HH2801 pKa = 5.38 STPALWLRR2809 pKa = 11.84 STAIRR2814 pKa = 11.84 YY2815 pKa = 7.16 YY2816 pKa = 10.16 QANKK2820 pKa = 9.45 EE2821 pKa = 3.84 NWRR2824 pKa = 11.84 TVSLARR2830 pKa = 11.84 TAGHH2834 pKa = 7.25 AGNCAFAPATYY2845 pKa = 10.5 LSFAHH2850 pKa = 6.37 EE2851 pKa = 4.33 WVEE2854 pKa = 4.82 DD2855 pKa = 3.82 ANFLLKK2861 pKa = 10.28 KK2862 pKa = 9.71 HH2863 pKa = 6.37 NIWTRR2868 pKa = 11.84 YY2869 pKa = 7.74 EE2870 pKa = 3.86 VSEE2873 pKa = 4.08 TRR2875 pKa = 11.84 GKK2877 pKa = 10.8 YY2878 pKa = 9.73 KK2879 pKa = 10.8 LPLVQEE2885 pKa = 4.5 ANPGAKK2891 pKa = 7.77 MTQALSPRR2899 pKa = 11.84 QKK2901 pKa = 11.28 AFLKK2905 pKa = 9.63 EE2906 pKa = 4.15 LKK2908 pKa = 10.86 GMMFPSYY2915 pKa = 10.23 LKK2917 pKa = 10.94 VMNVHH2922 pKa = 6.34 MNTADD2927 pKa = 3.68 IDD2929 pKa = 4.11 PEE2931 pKa = 4.0 AHH2933 pKa = 6.87 DD2934 pKa = 4.4 KK2935 pKa = 11.47 LFIKK2939 pKa = 10.39 LDD2941 pKa = 3.9 KK2942 pKa = 10.42 SWKK2945 pKa = 9.66 GPNEE2949 pKa = 3.87 VMAEE2953 pKa = 4.09 FADD2956 pKa = 3.74 QLQQFTDD2963 pKa = 4.84 MIPDD2967 pKa = 4.33 DD2968 pKa = 3.95 YY2969 pKa = 11.02 RR2970 pKa = 11.84 KK2971 pKa = 10.36 FMTGPSLQFAEE2982 pKa = 4.3 KK2983 pKa = 10.01 TFYY2986 pKa = 9.37 TKK2988 pKa = 11.2 NMLLEE2993 pKa = 4.32 KK2994 pKa = 9.9 FTYY2997 pKa = 10.21 KK2998 pKa = 10.58 QMLLEE3003 pKa = 4.9 SGDD3006 pKa = 4.09 DD3007 pKa = 3.86 EE3008 pKa = 4.4 ITFGDD3013 pKa = 3.74 FSEE3016 pKa = 5.19 RR3017 pKa = 11.84 IRR3019 pKa = 11.84 RR3020 pKa = 11.84 GPYY3023 pKa = 9.72 ACATDD3028 pKa = 3.89 PYY3030 pKa = 11.58 GFFEE3034 pKa = 5.35 KK3035 pKa = 10.65 KK3036 pKa = 10.63 NNDD3039 pKa = 3.49 PAFLKK3044 pKa = 10.31 EE3045 pKa = 3.94 VHH3047 pKa = 7.18 DD3048 pKa = 4.37 CNEE3051 pKa = 3.65 WLIQGLVFWITFIYY3065 pKa = 10.47 ALTPIVEE3072 pKa = 4.24 GFILSLWFFGPVYY3085 pKa = 9.78 KK3086 pKa = 9.81 IWMWSFFGLGKK3097 pKa = 10.65 LYY3099 pKa = 11.06 ALLNTLYY3106 pKa = 9.3 WHH3108 pKa = 6.74 SKK3110 pKa = 9.04 ANSSAEE3116 pKa = 4.18 ISRR3119 pKa = 11.84 MMPKK3123 pKa = 10.15 DD3124 pKa = 3.59 PYY3126 pKa = 10.32 MMSKK3130 pKa = 9.74 RR3131 pKa = 11.84 ACVFIVDD3138 pKa = 4.16 VFPQNFGFIMMVPCAIVNLIPEE3160 pKa = 4.63 LCEE3163 pKa = 4.41 AIGKK3167 pKa = 9.83 INFKK3171 pKa = 8.82 ATQMKK3176 pKa = 10.47 EE3177 pKa = 3.73 PDD3179 pKa = 3.81 TGNMPTEE3186 pKa = 4.49 NSWSRR3191 pKa = 11.84 YY3192 pKa = 7.9 AEE3194 pKa = 4.26 EE3195 pKa = 4.35 YY3196 pKa = 11.28 LDD3198 pKa = 4.43 ILWDD3202 pKa = 3.69 SPTRR3206 pKa = 11.84 SAYY3209 pKa = 10.13 LAADD3213 pKa = 3.74 TGTGKK3218 pKa = 10.69 SSWWLAALYY3227 pKa = 9.45 GARR3230 pKa = 11.84 RR3231 pKa = 11.84 HH3232 pKa = 5.71 KK3233 pKa = 10.5 NIRR3236 pKa = 11.84 HH3237 pKa = 4.49 VWVVSPYY3244 pKa = 10.73 KK3245 pKa = 10.33 SLRR3248 pKa = 11.84 DD3249 pKa = 3.89 NIDD3252 pKa = 2.97 VPFGIKK3258 pKa = 8.37 TQVLMKK3264 pKa = 10.08 GVQMNNDD3271 pKa = 3.53 FVKK3274 pKa = 10.55 SATYY3278 pKa = 10.49 GHH3280 pKa = 6.98 FAQARR3285 pKa = 11.84 INQIDD3290 pKa = 3.89 PEE3292 pKa = 4.49 RR3293 pKa = 11.84 DD3294 pKa = 3.18 VVLFDD3299 pKa = 3.89 EE3300 pKa = 5.18 FHH3302 pKa = 6.35 LQTMEE3307 pKa = 4.94 IINALHH3313 pKa = 6.48 FNPARR3318 pKa = 11.84 TFLLSATPVDD3328 pKa = 4.31 VPSLKK3333 pKa = 9.15 NTPSLFPDD3341 pKa = 3.57 IKK3343 pKa = 10.74 RR3344 pKa = 11.84 RR3345 pKa = 11.84 FQPVVKK3351 pKa = 10.5 LYY3353 pKa = 10.59 DD3354 pKa = 3.8 DD3355 pKa = 3.89 NMDD3358 pKa = 3.32 VVDD3361 pKa = 4.83 AYY3363 pKa = 10.18 KK3364 pKa = 10.28 EE3365 pKa = 4.33 AEE3367 pKa = 4.35 HH3368 pKa = 6.55 LWPHH3372 pKa = 5.96 VMKK3375 pKa = 10.69 KK3376 pKa = 8.67 PQPRR3380 pKa = 11.84 VLIVVPTIKK3389 pKa = 10.18 QQSDD3393 pKa = 4.49 TITLLQDD3400 pKa = 3.81 LLPNGTLINPYY3411 pKa = 10.31 SRR3413 pKa = 11.84 LHH3415 pKa = 6.28 RR3416 pKa = 11.84 NEE3418 pKa = 4.02 PPEE3421 pKa = 4.47 GIIVSTPYY3429 pKa = 10.78 VDD3431 pKa = 3.76 VGTNFKK3437 pKa = 10.72 NPPDD3441 pKa = 3.81 VLIDD3445 pKa = 3.51 AGKK3448 pKa = 10.05 QVLIDD3453 pKa = 3.85 RR3454 pKa = 11.84 GKK3456 pKa = 10.4 MILPLPWTDD3465 pKa = 3.84 PDD3467 pKa = 3.65 TDD3469 pKa = 3.32 KK3470 pKa = 11.08 QRR3472 pKa = 11.84 QGRR3475 pKa = 11.84 VARR3478 pKa = 11.84 KK3479 pKa = 8.14 GAGYY3483 pKa = 10.03 VFKK3486 pKa = 10.13 PHH3488 pKa = 6.75 SAGTGPKK3495 pKa = 9.41 GVAYY3499 pKa = 10.54 SSPSYY3504 pKa = 10.4 FAFKK3508 pKa = 10.42 HH3509 pKa = 4.92 VANHH3513 pKa = 6.6 FKK3515 pKa = 10.32 IQQLGSAPRR3524 pKa = 11.84 PACKK3528 pKa = 10.22 VMPWLGFNEE3537 pKa = 4.12 TDD3539 pKa = 3.61 ISIAEE3544 pKa = 4.3 KK3545 pKa = 10.6 KK3546 pKa = 10.38 SVALLHH3552 pKa = 6.21 AMAYY3556 pKa = 10.28 AGIKK3560 pKa = 8.86 EE3561 pKa = 4.35 SQWQKK3566 pKa = 10.39 FYY3568 pKa = 11.26 NIKK3571 pKa = 10.0 FQNKK3575 pKa = 8.39 PLSEE3579 pKa = 4.8 DD3580 pKa = 3.69 YY3581 pKa = 11.29 VFVDD3585 pKa = 4.74 RR3586 pKa = 11.84 VHH3588 pKa = 7.62 ADD3590 pKa = 3.55 YY3591 pKa = 10.92 QWNDD3595 pKa = 3.37 VQLLPYY3601 pKa = 8.35 TQARR3605 pKa = 11.84 MTYY3608 pKa = 8.28 NRR3610 pKa = 11.84 QGITSTFFKK3619 pKa = 10.57 RR3620 pKa = 11.84 RR3621 pKa = 11.84 TDD3623 pKa = 3.34 GAHH3626 pKa = 4.48 QEE3628 pKa = 4.14 EE3629 pKa = 4.82 RR3630 pKa = 11.84 LGRR3633 pKa = 11.84 PFKK3636 pKa = 10.81 PMGHH3640 pKa = 6.19 IWVQYY3645 pKa = 10.61 GSTLEE3650 pKa = 4.12 EE3651 pKa = 3.99 NDD3653 pKa = 4.8 DD3654 pKa = 3.81 ISVHH3658 pKa = 5.39 RR3659 pKa = 11.84 TDD3661 pKa = 4.61 ANTSIWGKK3669 pKa = 10.49 LNSDD3673 pKa = 3.86 LVKK3676 pKa = 10.29 TRR3678 pKa = 11.84 AALRR3682 pKa = 11.84 NMARR3686 pKa = 11.84 KK3687 pKa = 8.5 MPWDD3691 pKa = 3.33 RR3692 pKa = 11.84 RR3693 pKa = 11.84 LEE3695 pKa = 4.11 VLSDD3699 pKa = 3.84 LDD3701 pKa = 3.82 GVNYY3705 pKa = 10.71
Molecular weight: 421.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.727
IPC2_protein 8.009
IPC_protein 7.805
Toseland 7.863
ProMoST 8.434
Dawson 8.668
Bjellqvist 8.799
Wikipedia 8.697
Rodwell 8.741
Grimsley 8.053
Solomon 8.682
Lehninger 8.668
Nozaki 8.887
DTASelect 8.624
Thurlkill 8.697
EMBOSS 8.829
Sillero 8.931
Patrickios 4.482
IPC_peptide 8.682
IPC2_peptide 7.556
IPC2.peptide.svr19 7.776
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
3881
176
3705
1940.5
220.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.55 ± 0.065
1.185 ± 0.434
5.514 ± 0.294
5.772 ± 0.418
4.715 ± 0.386
5.978 ± 0.336
3.169 ± 0.323
5.437 ± 0.583
5.823 ± 0.17
8.374 ± 0.74
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.912 ± 0.482
4.148 ± 0.749
5.437 ± 0.81
3.247 ± 0.616
6.467 ± 0.367
6.596 ± 0.365
5.797 ± 0.408
6.081 ± 0.159
2.061 ± 0.084
3.736 ± 0.131
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here