Capybara microvirus Cap1_SP_51
Average proteome isoelectric point is 5.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W7G7|A0A4P8W7G7_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_51 OX=2584781 PE=4 SV=1
MM1 pKa = 7.69 SSFLTRR7 pKa = 11.84 LLNPIKK13 pKa = 10.52 LPIISIPLSFIEE25 pKa = 5.13 KK26 pKa = 10.4 GVDD29 pKa = 3.33 LFKK32 pKa = 11.07 DD33 pKa = 3.51 SSSLSEE39 pKa = 3.52 ITIGSKK45 pKa = 10.28 SLNDD49 pKa = 3.7 TTLGSYY55 pKa = 10.51 LGLTPSDD62 pKa = 3.46 AAEE65 pKa = 4.19 SAEE68 pKa = 4.03 SSSFVIDD75 pKa = 3.73 GVTFSADD82 pKa = 2.81 NQYY85 pKa = 10.29 AQNYY89 pKa = 6.98 IARR92 pKa = 11.84 FGYY95 pKa = 9.93 SSPRR99 pKa = 11.84 VLSSQLQKK107 pKa = 11.13 SLWEE111 pKa = 3.78 QAVSYY116 pKa = 7.94 EE117 pKa = 4.01 QNKK120 pKa = 10.13 IITDD124 pKa = 3.47 NQRR127 pKa = 11.84 EE128 pKa = 4.08 YY129 pKa = 11.18 DD130 pKa = 3.89 SEE132 pKa = 4.4 SSQVEE137 pKa = 4.09 RR138 pKa = 11.84 LQEE141 pKa = 3.88 AGINADD147 pKa = 3.5 LSGLSGVSGISQSATSPSDD166 pKa = 3.19 TDD168 pKa = 4.28 DD169 pKa = 3.24 VSNLINAGSGVVGLASGLFSGIFGGIQSIISIRR202 pKa = 11.84 KK203 pKa = 8.13 QLAEE207 pKa = 4.11 TEE209 pKa = 4.28 ATKK212 pKa = 11.09 SNTFGGVVKK221 pKa = 10.44 LAQEE225 pKa = 4.33 RR226 pKa = 11.84 ADD228 pKa = 3.67 TMSGISITEE237 pKa = 4.06 DD238 pKa = 2.98 QDD240 pKa = 3.69 GLKK243 pKa = 10.79 FNGNLYY249 pKa = 9.89 PDD251 pKa = 3.42 WNSFYY256 pKa = 11.01 DD257 pKa = 3.51 ANTDD261 pKa = 3.79 LFADD265 pKa = 4.27 DD266 pKa = 5.54 FSYY269 pKa = 10.88 LPKK272 pKa = 10.25 HH273 pKa = 5.6 LQSVYY278 pKa = 10.37 GKK280 pKa = 10.52 AFFGRR285 pKa = 11.84 FRR287 pKa = 11.84 SAAGRR292 pKa = 11.84 AAAARR297 pKa = 11.84 NQLAANKK304 pKa = 9.24 GASEE308 pKa = 5.14 LIMDD312 pKa = 4.94 QSFDD316 pKa = 3.78 PANTSDD322 pKa = 4.13 LFKK325 pKa = 10.2 MAHH328 pKa = 4.46 EE329 pKa = 4.51 TFRR332 pKa = 11.84 NSSSLSWLTTQIEE345 pKa = 4.26 LQIKK349 pKa = 9.83 QSLANSQIDD358 pKa = 3.52 YY359 pKa = 9.77 GGTMMTYY366 pKa = 11.09 ADD368 pKa = 5.05 LYY370 pKa = 11.43 AMNEE374 pKa = 4.24 TSSLQYY380 pKa = 10.85 DD381 pKa = 3.87 ITRR384 pKa = 11.84 SLADD388 pKa = 3.42 EE389 pKa = 3.82 QVYY392 pKa = 10.5 ILEE395 pKa = 4.49 KK396 pKa = 10.21 EE397 pKa = 3.92 LRR399 pKa = 11.84 LLVEE403 pKa = 4.0 QDD405 pKa = 3.01 EE406 pKa = 4.8 AMTSRR411 pKa = 11.84 IHH413 pKa = 7.06 SSLINSINNDD423 pKa = 3.1 FEE425 pKa = 4.65 SPYY428 pKa = 10.09 IRR430 pKa = 11.84 SILLSALFSNPEE442 pKa = 3.74 KK443 pKa = 10.61 VASDD447 pKa = 3.39 VVNNVITAAIGSALVLL463 pKa = 3.91
Molecular weight: 50.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.516
IPC2_protein 4.444
IPC_protein 4.418
Toseland 4.24
ProMoST 4.533
Dawson 4.368
Bjellqvist 4.52
Wikipedia 4.266
Rodwell 4.24
Grimsley 4.139
Solomon 4.368
Lehninger 4.329
Nozaki 4.482
DTASelect 4.673
Thurlkill 4.253
EMBOSS 4.279
Sillero 4.533
Patrickios 3.872
IPC_peptide 4.368
IPC2_peptide 4.52
IPC2.peptide.svr19 4.463
Protein with the highest isoelectric point:
>tr|A0A4P8W4W2|A0A4P8W4W2_9VIRU Replication initiator protein OS=Capybara microvirus Cap1_SP_51 OX=2584781 PE=4 SV=1
MM1 pKa = 7.95 SILNLFNFFSKK12 pKa = 10.52 FKK14 pKa = 10.32 PFLKK18 pKa = 9.95 KK19 pKa = 10.56 VYY21 pKa = 10.4 SFTPLVLSVIAIFISLSCLRR41 pKa = 11.84 VSRR44 pKa = 11.84 SALEE48 pKa = 4.15 KK49 pKa = 10.34 SSHH52 pKa = 5.58 AVEE55 pKa = 4.02 ICSNYY60 pKa = 10.37 SSDD63 pKa = 3.06 ISSLVDD69 pKa = 3.24 EE70 pKa = 4.49 VRR72 pKa = 11.84 KK73 pKa = 7.51 MTEE76 pKa = 3.89 TIEE79 pKa = 4.5 NRR81 pKa = 11.84 LDD83 pKa = 3.57 EE84 pKa = 4.33 QNEE87 pKa = 4.28 MLSHH91 pKa = 7.07 IYY93 pKa = 10.39 GDD95 pKa = 4.01 YY96 pKa = 10.67 FISIYY101 pKa = 11.23 GNGNKK106 pKa = 9.24 VDD108 pKa = 3.79 GKK110 pKa = 10.68 NFGKK114 pKa = 9.3 TSKK117 pKa = 10.58 KK118 pKa = 10.52
Molecular weight: 13.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.158
IPC2_protein 8.112
IPC_protein 8.17
Toseland 8.609
ProMoST 8.799
Dawson 8.902
Bjellqvist 8.887
Wikipedia 8.96
Rodwell 9.004
Grimsley 8.521
Solomon 9.151
Lehninger 9.136
Nozaki 9.033
DTASelect 8.756
Thurlkill 8.858
EMBOSS 9.092
Sillero 9.077
Patrickios 4.558
IPC_peptide 9.136
IPC2_peptide 7.658
IPC2.peptide.svr19 7.669
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1928
89
784
385.6
43.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.135 ± 1.102
1.297 ± 0.52
6.795 ± 0.339
4.305 ± 0.637
7.417 ± 0.911
4.824 ± 0.916
1.815 ± 0.666
5.965 ± 0.613
4.824 ± 1.016
8.766 ± 0.624
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.49 ± 0.408
6.587 ± 0.765
4.253 ± 0.878
2.645 ± 0.799
4.409 ± 0.51
12.033 ± 1.226
4.876 ± 0.502
5.913 ± 0.625
0.467 ± 0.083
5.187 ± 0.612
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here