Mycobacterium phage Echild

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 100 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W0LNN3|W0LNN3_9CAUD DUF2786 domain-containing protein OS=Mycobacterium phage Echild OX=1437839 GN=87 PE=4 SV=1
MM1 pKa = 7.71TIGDD5 pKa = 3.59IEE7 pKa = 4.93KK8 pKa = 10.2IRR10 pKa = 11.84EE11 pKa = 4.09AYY13 pKa = 10.3DD14 pKa = 3.28GFKK17 pKa = 11.22AMLDD21 pKa = 3.5AKK23 pKa = 10.55QEE25 pKa = 4.07FMDD28 pKa = 4.42EE29 pKa = 4.45HH30 pKa = 8.18NDD32 pKa = 3.57DD33 pKa = 5.03DD34 pKa = 5.51GNEE37 pKa = 4.11PEE39 pKa = 5.06CGYY42 pKa = 9.87EE43 pKa = 3.93VWDD46 pKa = 5.04EE47 pKa = 4.4EE48 pKa = 4.23IADD51 pKa = 4.03YY52 pKa = 11.2NHH54 pKa = 6.32EE55 pKa = 3.97LAYY58 pKa = 10.83LGEE61 pKa = 4.37LLFDD65 pKa = 4.3AVSKK69 pKa = 11.11ALGIKK74 pKa = 9.79EE75 pKa = 4.05DD76 pKa = 3.74SS77 pKa = 3.52

Molecular weight: 8.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W0LNU0|W0LNU0_9CAUD Holin OS=Mycobacterium phage Echild OX=1437839 GN=10 PE=4 SV=1
MM1 pKa = 7.29SWASSRR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84YY10 pKa = 9.77DD11 pKa = 4.66LPPDD15 pKa = 3.14WEE17 pKa = 4.41LNYY20 pKa = 10.26RR21 pKa = 11.84LPVLRR26 pKa = 11.84DD27 pKa = 3.59ANWICEE33 pKa = 4.27LQWNGCVGVASEE45 pKa = 4.24VDD47 pKa = 3.59HH48 pKa = 6.9IKK50 pKa = 10.61RR51 pKa = 11.84GNDD54 pKa = 3.11HH55 pKa = 6.61SRR57 pKa = 11.84SNLQAVCHH65 pKa = 6.16RR66 pKa = 11.84CHH68 pKa = 6.56AKK70 pKa = 10.26KK71 pKa = 10.66SSAEE75 pKa = 3.83GNARR79 pKa = 11.84KK80 pKa = 10.1AEE82 pKa = 3.73LRR84 pKa = 11.84ARR86 pKa = 11.84RR87 pKa = 11.84KK88 pKa = 10.31RR89 pKa = 11.84PDD91 pKa = 2.97EE92 pKa = 3.82RR93 pKa = 11.84HH94 pKa = 5.48PGRR97 pKa = 11.84RR98 pKa = 3.44

Molecular weight: 11.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

100

0

100

16647

20

845

166.5

18.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.834 ± 0.33

0.919 ± 0.108

6.512 ± 0.21

6.926 ± 0.346

3.304 ± 0.146

7.851 ± 0.253

2.042 ± 0.193

4.962 ± 0.178

4.74 ± 0.203

8.524 ± 0.325

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.343 ± 0.127

3.292 ± 0.23

5.496 ± 0.218

3.586 ± 0.19

6.614 ± 0.413

5.184 ± 0.236

6.151 ± 0.291

6.926 ± 0.202

1.952 ± 0.131

2.841 ± 0.157
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski