Amino acid dipepetide frequency for Mycobacterium phage Echild

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.934AlaAla: 10.934 ± 0.944
0.541AlaCys: 0.541 ± 0.167
5.106AlaAsp: 5.106 ± 0.498
7.329AlaGlu: 7.329 ± 0.803
3.845AlaPhe: 3.845 ± 0.558
7.149AlaGly: 7.149 ± 0.693
1.502AlaHis: 1.502 ± 0.302
4.145AlaIle: 4.145 ± 0.542
5.767AlaLys: 5.767 ± 0.51
9.011AlaLeu: 9.011 ± 0.931
2.283AlaMet: 2.283 ± 0.418
3.244AlaAsn: 3.244 ± 0.499
5.407AlaPro: 5.407 ± 0.635
3.725AlaGln: 3.725 ± 0.554
6.548AlaArg: 6.548 ± 0.621
5.046AlaSer: 5.046 ± 0.483
5.106AlaThr: 5.106 ± 0.759
7.87AlaVal: 7.87 ± 0.712
1.562AlaTrp: 1.562 ± 0.342
2.223AlaTyr: 2.223 ± 0.434
0.0AlaXaa: 0.0 ± 0.0
Cys
0.601CysAla: 0.601 ± 0.172
0.06CysCys: 0.06 ± 0.063
0.841CysAsp: 0.841 ± 0.267
0.541CysGlu: 0.541 ± 0.158
0.3CysPhe: 0.3 ± 0.145
0.901CysGly: 0.901 ± 0.227
0.421CysHis: 0.421 ± 0.168
0.18CysIle: 0.18 ± 0.098
0.421CysLys: 0.421 ± 0.159
1.021CysLeu: 1.021 ± 0.251
0.12CysMet: 0.12 ± 0.09
0.36CysAsn: 0.36 ± 0.129
0.481CysPro: 0.481 ± 0.216
0.12CysGln: 0.12 ± 0.08
0.481CysArg: 0.481 ± 0.154
0.421CysSer: 0.421 ± 0.17
0.601CysThr: 0.601 ± 0.157
0.721CysVal: 0.721 ± 0.208
0.3CysTrp: 0.3 ± 0.129
0.3CysTyr: 0.3 ± 0.126
0.0CysXaa: 0.0 ± 0.0
Asp
7.269AspAla: 7.269 ± 0.771
0.541AspCys: 0.541 ± 0.174
3.965AspAsp: 3.965 ± 0.546
4.926AspGlu: 4.926 ± 0.641
2.643AspPhe: 2.643 ± 0.457
5.347AspGly: 5.347 ± 0.559
1.682AspHis: 1.682 ± 0.313
3.604AspIle: 3.604 ± 0.439
2.163AspLys: 2.163 ± 0.341
5.106AspLeu: 5.106 ± 0.535
1.622AspMet: 1.622 ± 0.275
1.622AspAsn: 1.622 ± 0.3
5.046AspPro: 5.046 ± 0.526
1.922AspGln: 1.922 ± 0.308
3.064AspArg: 3.064 ± 0.424
3.304AspSer: 3.304 ± 0.424
3.184AspThr: 3.184 ± 0.432
4.506AspVal: 4.506 ± 0.488
1.141AspTrp: 1.141 ± 0.277
2.463AspTyr: 2.463 ± 0.325
0.0AspXaa: 0.0 ± 0.0
Glu
7.149GluAla: 7.149 ± 0.855
0.36GluCys: 0.36 ± 0.153
4.926GluAsp: 4.926 ± 0.69
4.446GluGlu: 4.446 ± 0.597
3.124GluPhe: 3.124 ± 0.432
4.986GluGly: 4.986 ± 0.627
1.982GluHis: 1.982 ± 0.389
3.965GluIle: 3.965 ± 0.499
2.223GluLys: 2.223 ± 0.33
7.449GluLeu: 7.449 ± 0.744
2.403GluMet: 2.403 ± 0.377
1.922GluAsn: 1.922 ± 0.302
2.824GluPro: 2.824 ± 0.347
1.922GluGln: 1.922 ± 0.349
4.926GluArg: 4.926 ± 0.625
3.364GluSer: 3.364 ± 0.387
4.325GluThr: 4.325 ± 0.531
3.905GluVal: 3.905 ± 0.551
1.262GluTrp: 1.262 ± 0.239
1.802GluTyr: 1.802 ± 0.307
0.0GluXaa: 0.0 ± 0.0
Phe
3.244PheAla: 3.244 ± 0.411
0.3PheCys: 0.3 ± 0.149
2.583PheAsp: 2.583 ± 0.485
2.403PheGlu: 2.403 ± 0.345
0.661PhePhe: 0.661 ± 0.205
3.064PheGly: 3.064 ± 0.497
0.841PheHis: 0.841 ± 0.295
1.442PheIle: 1.442 ± 0.319
1.922PheLys: 1.922 ± 0.305
2.763PheLeu: 2.763 ± 0.489
0.721PheMet: 0.721 ± 0.193
1.802PheAsn: 1.802 ± 0.326
1.562PhePro: 1.562 ± 0.334
0.961PheGln: 0.961 ± 0.215
1.922PheArg: 1.922 ± 0.32
1.562PheSer: 1.562 ± 0.255
2.043PheThr: 2.043 ± 0.303
2.163PheVal: 2.163 ± 0.386
0.36PheTrp: 0.36 ± 0.165
0.721PheTyr: 0.721 ± 0.185
0.0PheXaa: 0.0 ± 0.0
Gly
6.128GlyAla: 6.128 ± 0.681
0.901GlyCys: 0.901 ± 0.238
5.347GlyAsp: 5.347 ± 0.609
4.325GlyGlu: 4.325 ± 0.593
3.364GlyPhe: 3.364 ± 0.383
6.608GlyGly: 6.608 ± 0.802
1.622GlyHis: 1.622 ± 0.312
3.845GlyIle: 3.845 ± 0.516
4.506GlyLys: 4.506 ± 0.448
6.668GlyLeu: 6.668 ± 0.732
1.442GlyMet: 1.442 ± 0.31
3.604GlyAsn: 3.604 ± 0.517
2.763GlyPro: 2.763 ± 0.453
3.244GlyGln: 3.244 ± 0.435
3.965GlyArg: 3.965 ± 0.423
3.845GlySer: 3.845 ± 0.46
5.226GlyThr: 5.226 ± 0.651
6.608GlyVal: 6.608 ± 0.794
1.622GlyTrp: 1.622 ± 0.323
2.884GlyTyr: 2.884 ± 0.362
0.0GlyXaa: 0.0 ± 0.0
His
1.322HisAla: 1.322 ± 0.292
0.3HisCys: 0.3 ± 0.127
1.742HisAsp: 1.742 ± 0.376
1.262HisGlu: 1.262 ± 0.279
0.541HisPhe: 0.541 ± 0.164
1.682HisGly: 1.682 ± 0.436
0.481HisHis: 0.481 ± 0.173
1.081HisIle: 1.081 ± 0.243
1.141HisLys: 1.141 ± 0.266
1.562HisLeu: 1.562 ± 0.35
0.36HisMet: 0.36 ± 0.135
0.481HisAsn: 0.481 ± 0.176
1.262HisPro: 1.262 ± 0.254
0.901HisGln: 0.901 ± 0.247
1.622HisArg: 1.622 ± 0.378
0.781HisSer: 0.781 ± 0.217
1.442HisThr: 1.442 ± 0.282
1.322HisVal: 1.322 ± 0.25
0.421HisTrp: 0.421 ± 0.15
0.721HisTyr: 0.721 ± 0.238
0.0HisXaa: 0.0 ± 0.0
Ile
5.467IleAla: 5.467 ± 0.638
0.721IleCys: 0.721 ± 0.183
3.785IleAsp: 3.785 ± 0.444
4.506IleGlu: 4.506 ± 0.53
1.081IlePhe: 1.081 ± 0.237
4.446IleGly: 4.446 ± 0.651
1.021IleHis: 1.021 ± 0.256
1.922IleIle: 1.922 ± 0.326
2.523IleLys: 2.523 ± 0.389
3.604IleLeu: 3.604 ± 0.489
0.541IleMet: 0.541 ± 0.188
2.343IleAsn: 2.343 ± 0.396
3.184IlePro: 3.184 ± 0.47
1.262IleGln: 1.262 ± 0.338
3.244IleArg: 3.244 ± 0.446
2.583IleSer: 2.583 ± 0.335
3.124IleThr: 3.124 ± 0.394
2.763IleVal: 2.763 ± 0.398
0.481IleTrp: 0.481 ± 0.167
1.021IleTyr: 1.021 ± 0.217
0.0IleXaa: 0.0 ± 0.0
Lys
5.767LysAla: 5.767 ± 0.622
0.24LysCys: 0.24 ± 0.155
2.343LysAsp: 2.343 ± 0.444
2.523LysGlu: 2.523 ± 0.382
0.841LysPhe: 0.841 ± 0.178
2.944LysGly: 2.944 ± 0.505
0.721LysHis: 0.721 ± 0.228
2.703LysIle: 2.703 ± 0.413
2.703LysLys: 2.703 ± 0.567
4.986LysLeu: 4.986 ± 0.591
0.961LysMet: 0.961 ± 0.227
1.442LysAsn: 1.442 ± 0.317
2.824LysPro: 2.824 ± 0.518
2.043LysGln: 2.043 ± 0.333
3.304LysArg: 3.304 ± 0.442
2.343LysSer: 2.343 ± 0.351
2.824LysThr: 2.824 ± 0.403
3.424LysVal: 3.424 ± 0.373
0.901LysTrp: 0.901 ± 0.226
1.562LysTyr: 1.562 ± 0.329
0.0LysXaa: 0.0 ± 0.0
Leu
8.711LeuAla: 8.711 ± 0.71
0.661LeuCys: 0.661 ± 0.209
5.887LeuAsp: 5.887 ± 0.634
6.128LeuGlu: 6.128 ± 0.723
2.343LeuPhe: 2.343 ± 0.255
5.827LeuGly: 5.827 ± 0.822
1.502LeuHis: 1.502 ± 0.355
3.845LeuIle: 3.845 ± 0.376
3.484LeuLys: 3.484 ± 0.476
6.248LeuLeu: 6.248 ± 0.79
2.824LeuMet: 2.824 ± 0.512
2.944LeuAsn: 2.944 ± 0.448
5.407LeuPro: 5.407 ± 0.445
2.944LeuGln: 2.944 ± 0.474
6.128LeuArg: 6.128 ± 0.747
5.226LeuSer: 5.226 ± 0.556
6.128LeuThr: 6.128 ± 0.87
5.287LeuVal: 5.287 ± 0.647
1.682LeuTrp: 1.682 ± 0.34
1.982LeuTyr: 1.982 ± 0.396
0.0LeuXaa: 0.0 ± 0.0
Met
2.403MetAla: 2.403 ± 0.297
0.06MetCys: 0.06 ± 0.053
1.141MetAsp: 1.141 ± 0.243
0.961MetGlu: 0.961 ± 0.214
0.421MetPhe: 0.421 ± 0.145
1.442MetGly: 1.442 ± 0.326
0.421MetHis: 0.421 ± 0.143
1.622MetIle: 1.622 ± 0.3
1.562MetLys: 1.562 ± 0.271
1.382MetLeu: 1.382 ± 0.276
0.481MetMet: 0.481 ± 0.153
0.781MetAsn: 0.781 ± 0.222
1.562MetPro: 1.562 ± 0.367
1.141MetGln: 1.141 ± 0.244
1.622MetArg: 1.622 ± 0.315
2.343MetSer: 2.343 ± 0.392
2.163MetThr: 2.163 ± 0.296
0.961MetVal: 0.961 ± 0.254
0.18MetTrp: 0.18 ± 0.107
0.781MetTyr: 0.781 ± 0.241
0.0MetXaa: 0.0 ± 0.0
Asn
3.004AsnAla: 3.004 ± 0.497
0.541AsnCys: 0.541 ± 0.174
2.223AsnAsp: 2.223 ± 0.344
2.103AsnGlu: 2.103 ± 0.354
0.961AsnPhe: 0.961 ± 0.274
3.424AsnGly: 3.424 ± 0.544
0.841AsnHis: 0.841 ± 0.187
1.442AsnIle: 1.442 ± 0.288
1.201AsnLys: 1.201 ± 0.302
3.544AsnLeu: 3.544 ± 0.522
0.601AsnMet: 0.601 ± 0.193
0.601AsnAsn: 0.601 ± 0.176
2.343AsnPro: 2.343 ± 0.414
0.901AsnGln: 0.901 ± 0.239
2.523AsnArg: 2.523 ± 0.467
1.502AsnSer: 1.502 ± 0.278
1.081AsnThr: 1.081 ± 0.254
2.163AsnVal: 2.163 ± 0.318
1.021AsnTrp: 1.021 ± 0.223
0.901AsnTyr: 0.901 ± 0.181
0.0AsnXaa: 0.0 ± 0.0
Pro
5.407ProAla: 5.407 ± 0.613
0.3ProCys: 0.3 ± 0.133
4.085ProAsp: 4.085 ± 0.488
5.226ProGlu: 5.226 ± 0.637
1.922ProPhe: 1.922 ± 0.399
4.265ProGly: 4.265 ± 0.561
1.201ProHis: 1.201 ± 0.242
2.403ProIle: 2.403 ± 0.353
2.403ProLys: 2.403 ± 0.494
3.544ProLeu: 3.544 ± 0.485
1.201ProMet: 1.201 ± 0.254
1.742ProAsn: 1.742 ± 0.293
2.403ProPro: 2.403 ± 0.497
1.682ProGln: 1.682 ± 0.327
3.544ProArg: 3.544 ± 0.53
2.583ProSer: 2.583 ± 0.409
4.205ProThr: 4.205 ± 0.674
3.905ProVal: 3.905 ± 0.526
1.262ProTrp: 1.262 ± 0.358
1.682ProTyr: 1.682 ± 0.315
0.0ProXaa: 0.0 ± 0.0
Gln
4.085GlnAla: 4.085 ± 0.545
0.18GlnCys: 0.18 ± 0.101
1.141GlnAsp: 1.141 ± 0.247
1.922GlnGlu: 1.922 ± 0.355
1.141GlnPhe: 1.141 ± 0.244
2.583GlnGly: 2.583 ± 0.49
0.661GlnHis: 0.661 ± 0.187
2.763GlnIle: 2.763 ± 0.34
1.742GlnLys: 1.742 ± 0.35
3.484GlnLeu: 3.484 ± 0.664
0.961GlnMet: 0.961 ± 0.216
0.721GlnAsn: 0.721 ± 0.168
1.262GlnPro: 1.262 ± 0.336
2.223GlnGln: 2.223 ± 0.444
2.523GlnArg: 2.523 ± 0.504
1.802GlnSer: 1.802 ± 0.347
2.403GlnThr: 2.403 ± 0.45
2.583GlnVal: 2.583 ± 0.325
1.021GlnTrp: 1.021 ± 0.29
0.661GlnTyr: 0.661 ± 0.189
0.0GlnXaa: 0.0 ± 0.0
Arg
5.407ArgAla: 5.407 ± 0.598
1.081ArgCys: 1.081 ± 0.26
4.385ArgAsp: 4.385 ± 0.529
5.647ArgGlu: 5.647 ± 0.805
2.283ArgPhe: 2.283 ± 0.386
4.385ArgGly: 4.385 ± 0.558
1.442ArgHis: 1.442 ± 0.25
4.025ArgIle: 4.025 ± 0.46
3.364ArgLys: 3.364 ± 0.52
5.166ArgLeu: 5.166 ± 0.511
1.682ArgMet: 1.682 ± 0.281
2.283ArgAsn: 2.283 ± 0.379
2.763ArgPro: 2.763 ± 0.364
2.343ArgGln: 2.343 ± 0.362
5.527ArgArg: 5.527 ± 0.624
3.004ArgSer: 3.004 ± 0.516
3.244ArgThr: 3.244 ± 0.504
4.746ArgVal: 4.746 ± 0.583
1.382ArgTrp: 1.382 ± 0.317
1.982ArgTyr: 1.982 ± 0.366
0.0ArgXaa: 0.0 ± 0.0
Ser
4.566SerAla: 4.566 ± 0.513
0.541SerCys: 0.541 ± 0.214
3.304SerAsp: 3.304 ± 0.443
3.124SerGlu: 3.124 ± 0.361
1.862SerPhe: 1.862 ± 0.314
4.986SerGly: 4.986 ± 0.584
1.081SerHis: 1.081 ± 0.252
1.982SerIle: 1.982 ± 0.32
2.283SerLys: 2.283 ± 0.361
4.205SerLeu: 4.205 ± 0.529
1.382SerMet: 1.382 ± 0.211
1.262SerAsn: 1.262 ± 0.242
3.484SerPro: 3.484 ± 0.485
2.223SerGln: 2.223 ± 0.376
3.965SerArg: 3.965 ± 0.526
2.583SerSer: 2.583 ± 0.413
3.424SerThr: 3.424 ± 0.424
2.824SerVal: 2.824 ± 0.4
1.262SerTrp: 1.262 ± 0.271
1.502SerTyr: 1.502 ± 0.321
0.0SerXaa: 0.0 ± 0.0
Thr
5.827ThrAla: 5.827 ± 0.666
0.36ThrCys: 0.36 ± 0.14
3.725ThrAsp: 3.725 ± 0.546
3.424ThrGlu: 3.424 ± 0.467
2.163ThrPhe: 2.163 ± 0.344
5.707ThrGly: 5.707 ± 0.618
0.841ThrHis: 0.841 ± 0.2
2.583ThrIle: 2.583 ± 0.404
3.124ThrLys: 3.124 ± 0.446
5.767ThrLeu: 5.767 ± 0.706
1.262ThrMet: 1.262 ± 0.242
1.502ThrAsn: 1.502 ± 0.245
4.626ThrPro: 4.626 ± 0.487
2.463ThrGln: 2.463 ± 0.406
2.944ThrArg: 2.944 ± 0.393
3.064ThrSer: 3.064 ± 0.461
3.665ThrThr: 3.665 ± 0.473
5.166ThrVal: 5.166 ± 0.68
1.262ThrTrp: 1.262 ± 0.306
2.043ThrTyr: 2.043 ± 0.459
0.0ThrXaa: 0.0 ± 0.0
Val
6.668ValAla: 6.668 ± 0.856
0.901ValCys: 0.901 ± 0.196
4.686ValAsp: 4.686 ± 0.481
4.926ValGlu: 4.926 ± 0.496
2.223ValPhe: 2.223 ± 0.435
4.866ValGly: 4.866 ± 0.472
1.141ValHis: 1.141 ± 0.231
3.725ValIle: 3.725 ± 0.641
3.364ValLys: 3.364 ± 0.423
5.407ValLeu: 5.407 ± 0.584
1.502ValMet: 1.502 ± 0.293
2.583ValAsn: 2.583 ± 0.375
3.064ValPro: 3.064 ± 0.531
2.223ValGln: 2.223 ± 0.351
4.446ValArg: 4.446 ± 0.521
3.965ValSer: 3.965 ± 0.529
4.746ValThr: 4.746 ± 0.482
5.347ValVal: 5.347 ± 0.501
1.442ValTrp: 1.442 ± 0.344
2.043ValTyr: 2.043 ± 0.445
0.0ValXaa: 0.0 ± 0.0
Trp
1.682TrpAla: 1.682 ± 0.36
0.36TrpCys: 0.36 ± 0.163
1.382TrpAsp: 1.382 ± 0.285
1.442TrpGlu: 1.442 ± 0.283
0.481TrpPhe: 0.481 ± 0.171
1.682TrpGly: 1.682 ± 0.289
0.541TrpHis: 0.541 ± 0.195
0.961TrpIle: 0.961 ± 0.211
0.721TrpLys: 0.721 ± 0.267
1.322TrpLeu: 1.322 ± 0.312
0.541TrpMet: 0.541 ± 0.168
0.781TrpAsn: 0.781 ± 0.206
1.021TrpPro: 1.021 ± 0.247
0.721TrpGln: 0.721 ± 0.203
1.262TrpArg: 1.262 ± 0.227
1.081TrpSer: 1.081 ± 0.289
1.201TrpThr: 1.201 ± 0.27
1.322TrpVal: 1.322 ± 0.231
0.421TrpTrp: 0.421 ± 0.157
0.601TrpTyr: 0.601 ± 0.21
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.703TyrAla: 2.703 ± 0.422
0.24TyrCys: 0.24 ± 0.141
2.523TyrAsp: 2.523 ± 0.334
2.103TyrGlu: 2.103 ± 0.322
0.841TyrPhe: 0.841 ± 0.184
2.223TyrGly: 2.223 ± 0.366
0.481TyrHis: 0.481 ± 0.139
1.382TyrIle: 1.382 ± 0.222
0.781TyrLys: 0.781 ± 0.234
3.004TyrLeu: 3.004 ± 0.357
0.481TyrMet: 0.481 ± 0.177
0.901TyrAsn: 0.901 ± 0.208
1.682TyrPro: 1.682 ± 0.324
0.901TyrGln: 0.901 ± 0.2
2.583TyrArg: 2.583 ± 0.481
1.442TyrSer: 1.442 ± 0.311
1.382TyrThr: 1.382 ± 0.271
1.682TyrVal: 1.682 ± 0.29
0.541TyrTrp: 0.541 ± 0.193
0.541TyrTyr: 0.541 ± 0.185
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 100 proteins (16647 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski