Vibrio mangrovi
Average proteome isoelectric point is 6.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4312 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y6IPN4|A0A1Y6IPN4_9VIBR Autoinducer 2-binding periplasmic protein LuxP OS=Vibrio mangrovi OX=474394 GN=lsrB PE=4 SV=1
MM1 pKa = 7.65 KK2 pKa = 8.99 KK3 pKa = 8.31 TLIALAVLAAGSASAAEE20 pKa = 5.09 IYY22 pKa = 10.35 NQDD25 pKa = 3.09 GVAVTVAGAGEE36 pKa = 4.23 VQLMQAYY43 pKa = 8.26 EE44 pKa = 3.89 QDD46 pKa = 3.72 GTDD49 pKa = 2.76 VSTKK53 pKa = 10.57 LRR55 pKa = 11.84 IDD57 pKa = 3.97 DD58 pKa = 3.93 MQLNTHH64 pKa = 5.6 GTIEE68 pKa = 4.2 VAEE71 pKa = 4.36 GLSAVVGLDD80 pKa = 3.24 FTFEE84 pKa = 4.78 DD85 pKa = 4.91 DD86 pKa = 4.02 SSASKK91 pKa = 11.35 GNVQTDD97 pKa = 3.33 GSYY100 pKa = 11.16 VGFAYY105 pKa = 9.64 TDD107 pKa = 3.55 YY108 pKa = 9.86 GTITYY113 pKa = 9.06 GRR115 pKa = 11.84 QYY117 pKa = 11.1 LITDD121 pKa = 3.79 DD122 pKa = 4.25 SGIGADD128 pKa = 4.88 FEE130 pKa = 4.76 TGKK133 pKa = 9.59 NQYY136 pKa = 9.25 GQEE139 pKa = 4.15 TTVGKK144 pKa = 10.26 DD145 pKa = 3.15 VIKK148 pKa = 10.68 YY149 pKa = 10.18 VYY151 pKa = 10.78 DD152 pKa = 3.29 NGTFYY157 pKa = 11.07 FGLSHH162 pKa = 7.54 DD163 pKa = 4.41 LDD165 pKa = 3.93 EE166 pKa = 6.31 SEE168 pKa = 4.6 GTTIDD173 pKa = 3.26 ATSTDD178 pKa = 2.86 GRR180 pKa = 11.84 IGFRR184 pKa = 11.84 YY185 pKa = 9.62 EE186 pKa = 3.74 GLDD189 pKa = 3.2 ARR191 pKa = 11.84 LYY193 pKa = 10.14 IYY195 pKa = 10.89 SGDD198 pKa = 3.94 DD199 pKa = 3.18 VDD201 pKa = 4.79 ISSTATGDD209 pKa = 3.31 EE210 pKa = 4.27 DD211 pKa = 4.5 SFNLEE216 pKa = 3.4 VTYY219 pKa = 11.0 EE220 pKa = 3.68 MDD222 pKa = 3.42 AFNFGASYY230 pKa = 10.57 GQTEE234 pKa = 4.26 QDD236 pKa = 2.86 NTAGVEE242 pKa = 4.13 VTDD245 pKa = 3.96 RR246 pKa = 11.84 DD247 pKa = 3.66 IIEE250 pKa = 4.5 LVGSYY255 pKa = 10.29 TMDD258 pKa = 2.85 KK259 pKa = 8.34 TTFSLGYY266 pKa = 9.11 VHH268 pKa = 6.95 NDD270 pKa = 3.17 DD271 pKa = 4.04 NTSVSNDD278 pKa = 2.59 GDD280 pKa = 3.54 NLYY283 pKa = 11.18 GVVVYY288 pKa = 10.69 KK289 pKa = 10.36 MNANVRR295 pKa = 11.84 TYY297 pKa = 11.45 AEE299 pKa = 3.95 VGFYY303 pKa = 10.83 DD304 pKa = 4.68 DD305 pKa = 6.16 DD306 pKa = 4.6 SNKK309 pKa = 10.73 DD310 pKa = 3.25 YY311 pKa = 11.53 DD312 pKa = 3.7 PAYY315 pKa = 8.79 TLGMEE320 pKa = 4.52 VKK322 pKa = 10.57 FF323 pKa = 4.41
Molecular weight: 35.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.732
IPC2_protein 3.745
IPC_protein 3.783
Toseland 3.554
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.719
Rodwell 3.605
Grimsley 3.452
Solomon 3.77
Lehninger 3.732
Nozaki 3.884
DTASelect 4.151
Thurlkill 3.605
EMBOSS 3.732
Sillero 3.897
Patrickios 0.642
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.805
Protein with the highest isoelectric point:
>tr|A0A1Y6IYJ9|A0A1Y6IYJ9_9VIBR Uncharacterized protein OS=Vibrio mangrovi OX=474394 GN=VIM7927_04064 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.5 RR3 pKa = 11.84 TFQPTVLKK11 pKa = 10.46 RR12 pKa = 11.84 KK13 pKa = 7.65 RR14 pKa = 11.84 THH16 pKa = 5.89 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.45 NGRR28 pKa = 11.84 KK29 pKa = 9.19 VLNARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.26 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LSKK44 pKa = 10.83
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.705
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.427
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4312
0
4312
1428354
29
4679
331.3
36.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.251 ± 0.036
1.126 ± 0.016
5.386 ± 0.036
6.237 ± 0.038
4.025 ± 0.027
6.831 ± 0.034
2.557 ± 0.02
6.573 ± 0.04
4.672 ± 0.041
10.315 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.654 ± 0.02
3.881 ± 0.026
4.096 ± 0.025
5.022 ± 0.032
4.935 ± 0.032
6.713 ± 0.031
5.374 ± 0.042
6.854 ± 0.029
1.262 ± 0.016
3.235 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here