Changjiang tombus-like virus 6

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KG36|A0A1L3KG36_9VIRU RNA-directed RNA polymerase OS=Changjiang tombus-like virus 6 OX=1922820 PE=4 SV=1
MM1 pKa = 7.3HH2 pKa = 7.64PVFPGLSGALRR13 pKa = 11.84FGVHH17 pKa = 6.25NNNLRR22 pKa = 11.84NGCRR26 pKa = 11.84ALVEE30 pKa = 4.2RR31 pKa = 11.84VFLRR35 pKa = 11.84EE36 pKa = 4.14DD37 pKa = 3.43NGNLIPPPPCVEE49 pKa = 4.46DD50 pKa = 3.46VNKK53 pKa = 9.55TLEE56 pKa = 4.03EE57 pKa = 4.01FKK59 pKa = 10.86AAVITRR65 pKa = 11.84VGVHH69 pKa = 5.88RR70 pKa = 11.84RR71 pKa = 11.84WSVDD75 pKa = 2.8KK76 pKa = 10.69FVNSYY81 pKa = 8.12TGRR84 pKa = 11.84RR85 pKa = 11.84QNVYY89 pKa = 10.58ARR91 pKa = 11.84AAKK94 pKa = 10.23SLEE97 pKa = 4.06TTPVHH102 pKa = 6.0TGDD105 pKa = 3.97FKK107 pKa = 11.88VMNAFVKK114 pKa = 10.65AEE116 pKa = 4.07KK117 pKa = 10.13TNLTSKK123 pKa = 10.16PDD125 pKa = 3.68PAPRR129 pKa = 11.84VIQPRR134 pKa = 11.84NPRR137 pKa = 11.84YY138 pKa = 8.64NVEE141 pKa = 3.56VGRR144 pKa = 11.84FLKK147 pKa = 10.79GLEE150 pKa = 4.05HH151 pKa = 6.66KK152 pKa = 10.39VYY154 pKa = 10.61KK155 pKa = 10.4AVEE158 pKa = 4.73DD159 pKa = 3.63IFGDD163 pKa = 3.61PTVMKK168 pKa = 9.99GYY170 pKa = 9.64NAEE173 pKa = 3.99EE174 pKa = 4.42VAEE177 pKa = 4.17HH178 pKa = 6.42MVSKK182 pKa = 9.3WQSFSDD188 pKa = 4.06PVAVGLDD195 pKa = 3.17ASRR198 pKa = 11.84FDD200 pKa = 3.35QHH202 pKa = 6.42VRR204 pKa = 11.84PSMLEE209 pKa = 3.93WEE211 pKa = 4.36HH212 pKa = 7.3SVYY215 pKa = 10.78AGCFSTGEE223 pKa = 3.93AKK225 pKa = 10.4LLKK228 pKa = 9.68WLLQGQIRR236 pKa = 11.84NVCTMRR242 pKa = 11.84VTDD245 pKa = 4.03GLVKK249 pKa = 10.68YY250 pKa = 9.92RR251 pKa = 11.84VDD253 pKa = 3.56GSRR256 pKa = 11.84MSGDD260 pKa = 3.28MNTALGNCLIMCALVWRR277 pKa = 11.84LGKK280 pKa = 10.52VLGIRR285 pKa = 11.84LKK287 pKa = 10.72LANNGDD293 pKa = 3.83DD294 pKa = 3.31CVVFMEE300 pKa = 4.96RR301 pKa = 11.84KK302 pKa = 10.13DD303 pKa = 3.37LTAFNNKK310 pKa = 8.35VKK312 pKa = 10.61GFFLDD317 pKa = 3.29YY318 pKa = 10.67GFTMKK323 pKa = 10.45VEE325 pKa = 4.17APSFFIEE332 pKa = 4.95QIEE335 pKa = 4.55FCQAKK340 pKa = 9.42PVWDD344 pKa = 3.75GCKK347 pKa = 9.59WIMVRR352 pKa = 11.84DD353 pKa = 3.86PRR355 pKa = 11.84VCTSKK360 pKa = 11.04DD361 pKa = 3.14ATCVVKK367 pKa = 10.62DD368 pKa = 3.58YY369 pKa = 10.91GYY371 pKa = 11.48GSAASYY377 pKa = 9.86WLGAVGEE384 pKa = 4.99CGLAMAGGIPVMQNYY399 pKa = 9.36YY400 pKa = 10.61SAFARR405 pKa = 11.84FGNTNRR411 pKa = 11.84QVQCVTEE418 pKa = 3.85TGMAYY423 pKa = 10.65LARR426 pKa = 11.84GLHH429 pKa = 6.21RR430 pKa = 11.84EE431 pKa = 4.07ATVISADD438 pKa = 3.12ARR440 pKa = 11.84VSFWRR445 pKa = 11.84AFGIAPTQQRR455 pKa = 11.84EE456 pKa = 4.17LEE458 pKa = 4.23KK459 pKa = 10.48WCDD462 pKa = 3.57NTSLTLPNSPCIRR475 pKa = 11.84AYY477 pKa = 9.29PPRR480 pKa = 11.84GILPLLL486 pKa = 3.91

Molecular weight: 54.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KG36|A0A1L3KG36_9VIRU RNA-directed RNA polymerase OS=Changjiang tombus-like virus 6 OX=1922820 PE=4 SV=1
MM1 pKa = 7.6AKK3 pKa = 10.07KK4 pKa = 10.35KK5 pKa = 10.12NSKK8 pKa = 9.75NKK10 pKa = 8.37TVIRR14 pKa = 11.84RR15 pKa = 11.84VAKK18 pKa = 10.27IPRR21 pKa = 11.84SPLDD25 pKa = 3.64AQGLAYY31 pKa = 10.18ARR33 pKa = 11.84LLADD37 pKa = 3.85PCNAPLVHH45 pKa = 6.36PVYY48 pKa = 10.83SGTEE52 pKa = 3.54GGYY55 pKa = 10.6LIRR58 pKa = 11.84ADD60 pKa = 3.46SFLTVGANALEE71 pKa = 4.17QAGVFQWVPGGIDD84 pKa = 3.28ALQDD88 pKa = 3.96NILLGAAANATTAINPGVSGAASPAWDD115 pKa = 4.03FLTNTASQVRR125 pKa = 11.84CVAACLRR132 pKa = 11.84IMYY135 pKa = 8.49PGSEE139 pKa = 4.16SSRR142 pKa = 11.84SGRR145 pKa = 11.84IAFGQASGALLKK157 pKa = 11.04SGVTVTANNLANTMAHH173 pKa = 5.83YY174 pKa = 10.69CRR176 pKa = 11.84TPADD180 pKa = 4.17EE181 pKa = 5.77IEE183 pKa = 4.84VVWKK187 pKa = 10.32PNDD190 pKa = 3.46ADD192 pKa = 3.57QLFRR196 pKa = 11.84NPALNGALQDD206 pKa = 3.82LDD208 pKa = 3.55RR209 pKa = 11.84RR210 pKa = 11.84AALVCSFAGLPAATGVVFRR229 pKa = 11.84MTAVYY234 pKa = 9.37EE235 pKa = 3.81WQPRR239 pKa = 11.84VNEE242 pKa = 4.95GIAVPNLSKK251 pKa = 11.17SMSSNTLDD259 pKa = 4.41DD260 pKa = 3.73VVNYY264 pKa = 9.72LIQRR268 pKa = 11.84GYY270 pKa = 11.58GFINGAAASAGHH282 pKa = 6.4GLSAGLISGISGVFGVMPSVTTSRR306 pKa = 11.84SRR308 pKa = 11.84RR309 pKa = 11.84VMGG312 pKa = 3.97

Molecular weight: 32.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1064

266

486

354.7

38.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.842 ± 2.031

2.538 ± 0.331

4.605 ± 0.158

3.947 ± 0.651

4.041 ± 0.471

8.459 ± 0.403

1.504 ± 0.198

3.853 ± 0.396

4.135 ± 0.727

7.425 ± 0.669

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.289 ± 0.593

4.511 ± 1.21

4.793 ± 0.368

2.82 ± 0.134

7.331 ± 0.643

5.545 ± 0.872

5.169 ± 0.174

9.492 ± 0.409

1.692 ± 0.187

3.008 ± 0.174
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski