Streptococcus phage D4276

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Moineauvirus; unclassified Moineauvirus

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A240FHV6|A0A240FHV6_9CAUD Uncharacterized protein OS=Streptococcus phage D4276 OX=2006928 GN=D4276_029 PE=4 SV=1
MM1 pKa = 7.11VNWVDD6 pKa = 4.52ADD8 pKa = 4.28GNDD11 pKa = 4.07LPDD14 pKa = 5.09GADD17 pKa = 3.18QDD19 pKa = 4.92FKK21 pKa = 11.67AGMYY25 pKa = 10.19FSFAGDD31 pKa = 3.52EE32 pKa = 4.3VNITDD37 pKa = 4.1TGDD40 pKa = 2.69GGYY43 pKa = 10.72YY44 pKa = 9.75GGYY47 pKa = 7.97YY48 pKa = 9.41YY49 pKa = 10.76RR50 pKa = 11.84RR51 pKa = 11.84FEE53 pKa = 4.06FGQFGTVWLSCWNKK67 pKa = 10.66DD68 pKa = 3.89DD69 pKa = 5.14LVNYY73 pKa = 7.38YY74 pKa = 8.26QQ75 pKa = 4.22

Molecular weight: 8.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A220GCE4|A0A220GCE4_9CAUD Uncharacterized protein OS=Streptococcus phage D4276 OX=2006928 GN=D4276_034 PE=4 SV=1
MM1 pKa = 7.56SKK3 pKa = 9.22TKK5 pKa = 10.5RR6 pKa = 11.84GVCANCHH13 pKa = 4.61TVFEE17 pKa = 4.53VSKK20 pKa = 10.01KK21 pKa = 6.75QRR23 pKa = 11.84RR24 pKa = 11.84KK25 pKa = 8.8IKK27 pKa = 8.88EE28 pKa = 4.09GKK30 pKa = 9.71SVFCSEE36 pKa = 3.81TCALEE41 pKa = 4.71KK42 pKa = 10.77YY43 pKa = 9.83GKK45 pKa = 8.07TKK47 pKa = 10.37IIISEE52 pKa = 4.3IPCCRR57 pKa = 11.84CGKK60 pKa = 9.28MFTPTYY66 pKa = 9.9NQYY69 pKa = 10.78KK70 pKa = 8.85RR71 pKa = 11.84YY72 pKa = 9.81KK73 pKa = 9.02YY74 pKa = 10.59NDD76 pKa = 3.28YY77 pKa = 10.92VSNSFCSNEE86 pKa = 3.99CRR88 pKa = 11.84WKK90 pKa = 10.73KK91 pKa = 10.23EE92 pKa = 4.07YY93 pKa = 9.51PCKK96 pKa = 10.6YY97 pKa = 8.82HH98 pKa = 7.57DD99 pKa = 5.03DD100 pKa = 4.04YY101 pKa = 12.18VSVFVNGKK109 pKa = 9.91EE110 pKa = 3.7ILLDD114 pKa = 3.54FDD116 pKa = 4.38VFEE119 pKa = 5.31KK120 pKa = 10.89YY121 pKa = 10.7SKK123 pKa = 9.54TLYY126 pKa = 9.73VRR128 pKa = 11.84NDD130 pKa = 2.66KK131 pKa = 10.86RR132 pKa = 11.84NNYY135 pKa = 8.28HH136 pKa = 5.82SVYY139 pKa = 10.47VFEE142 pKa = 4.88KK143 pKa = 10.23GKK145 pKa = 10.72KK146 pKa = 8.36ILSRR150 pKa = 11.84LIMSVADD157 pKa = 3.41KK158 pKa = 11.07NKK160 pKa = 10.77YY161 pKa = 8.8IDD163 pKa = 4.82HH164 pKa = 7.04INGNPLDD171 pKa = 3.85NRR173 pKa = 11.84RR174 pKa = 11.84SNLRR178 pKa = 11.84VVSHH182 pKa = 5.96QEE184 pKa = 3.05NMMNKK189 pKa = 8.25KK190 pKa = 8.41TYY192 pKa = 10.6KK193 pKa = 10.75NNTSKK198 pKa = 10.74IKK200 pKa = 10.53GVNLNKK206 pKa = 10.19KK207 pKa = 8.12GLWVARR213 pKa = 11.84IQVRR217 pKa = 11.84NQRR220 pKa = 11.84IFLGSSKK227 pKa = 10.56DD228 pKa = 3.26KK229 pKa = 11.18SVAEE233 pKa = 4.0RR234 pKa = 11.84LRR236 pKa = 11.84IEE238 pKa = 4.16AEE240 pKa = 3.57KK241 pKa = 10.62KK242 pKa = 10.51YY243 pKa = 10.45FGKK246 pKa = 10.56YY247 pKa = 8.89DD248 pKa = 3.6RR249 pKa = 11.84KK250 pKa = 10.04FLKK253 pKa = 10.59

Molecular weight: 30.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

12365

44

1530

229.0

26.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.179 ± 0.402

0.663 ± 0.133

6.373 ± 0.248

6.721 ± 0.43

4.214 ± 0.204

6.543 ± 0.461

1.52 ± 0.092

6.68 ± 0.279

8.799 ± 0.447

7.966 ± 0.338

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.564 ± 0.171

6.429 ± 0.264

2.822 ± 0.19

4.351 ± 0.208

4.108 ± 0.263

6.235 ± 0.211

6.227 ± 0.28

5.718 ± 0.217

1.456 ± 0.139

4.432 ± 0.306
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski