Marinitoga camini virus 1
Average proteome isoelectric point is 7.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A139ZPG3|A0A139ZPG3_9CAUD Chitin binding protein OS=Marinitoga camini virus 1 OX=1629954 GN=UF08_36 PE=4 SV=1
MM1 pKa = 7.5 ALVKK5 pKa = 9.8 TDD7 pKa = 3.32 YY8 pKa = 11.54 GLIYY12 pKa = 10.55 NDD14 pKa = 5.02 DD15 pKa = 3.69 GSLCVTVRR23 pKa = 11.84 DD24 pKa = 3.97 DD25 pKa = 5.3 DD26 pKa = 4.01 IPFEE30 pKa = 4.35 GVKK33 pKa = 10.46 SFAVEE38 pKa = 3.87 EE39 pKa = 4.69 GTTNLITGDD48 pKa = 3.57 SATFATSIGSWVEE61 pKa = 3.29 SDD63 pKa = 3.91 ANLTITWEE71 pKa = 4.1 NIYY74 pKa = 11.21 NEE76 pKa = 4.05 FLKK79 pKa = 11.02 QNGVMRR85 pKa = 11.84 YY86 pKa = 9.57 RR87 pKa = 11.84 KK88 pKa = 7.88 TDD90 pKa = 3.28 SNNASCRR97 pKa = 11.84 INITDD102 pKa = 3.67 STLTTYY108 pKa = 10.58 SGSIYY113 pKa = 10.24 IKK115 pKa = 10.47 LISGNINAIRR125 pKa = 11.84 PAGVFYY131 pKa = 11.2 YY132 pKa = 10.69 SDD134 pKa = 3.93 NTWTDD139 pKa = 3.65 YY140 pKa = 11.53 SWVDD144 pKa = 3.11 ANGSYY149 pKa = 10.58 KK150 pKa = 10.55 RR151 pKa = 11.84 IIDD154 pKa = 3.87 MGNGIYY160 pKa = 10.18 RR161 pKa = 11.84 IEE163 pKa = 4.27 VNNMKK168 pKa = 10.15 YY169 pKa = 10.57 DD170 pKa = 3.48 QTKK173 pKa = 9.74 IDD175 pKa = 3.66 AGLTISKK182 pKa = 8.96 FQIRR186 pKa = 11.84 ITSPDD191 pKa = 3.26 LVDD194 pKa = 5.0 FYY196 pKa = 12.01 VLAAQWEE203 pKa = 4.4 QKK205 pKa = 10.27 PFATSFVDD213 pKa = 3.74 GTRR216 pKa = 11.84 ADD218 pKa = 4.17 GNLQLPWTMDD228 pKa = 2.99 GYY230 pKa = 11.68 NFVINLFAKK239 pKa = 10.6 DD240 pKa = 4.35 LFSSTNTNTHH250 pKa = 6.21 IFNLGTTGLTDD261 pKa = 3.36 VYY263 pKa = 10.79 DD264 pKa = 3.57 GRR266 pKa = 11.84 LILRR270 pKa = 11.84 QNNSLFQLYY279 pKa = 9.63 IDD281 pKa = 4.84 PADD284 pKa = 3.94 GSASVSSNTSVASNDD299 pKa = 3.46 GKK301 pKa = 10.96 YY302 pKa = 10.6 HH303 pKa = 6.0 MLTVLVEE310 pKa = 4.09 ATQARR315 pKa = 11.84 LYY317 pKa = 10.49 IDD319 pKa = 5.51 GIYY322 pKa = 8.87 KK323 pKa = 7.77 TTLTYY328 pKa = 11.18 DD329 pKa = 3.5 FTKK332 pKa = 10.88 EE333 pKa = 4.01 NFDD336 pKa = 4.04 KK337 pKa = 11.15 LVLGSNISFSPVTAYY352 pKa = 10.69 GLISNLYY359 pKa = 8.84 IGNYY363 pKa = 9.4 DD364 pKa = 3.59 PNIWTDD370 pKa = 3.28 AFIQEE375 pKa = 4.98 LYY377 pKa = 8.71 NAKK380 pKa = 10.26 RR381 pKa = 11.84 PFSVPAKK388 pKa = 10.53 LPIII392 pKa = 4.4
Molecular weight: 43.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.398
IPC2_protein 4.507
IPC_protein 4.495
Toseland 4.291
ProMoST 4.647
Dawson 4.482
Bjellqvist 4.635
Wikipedia 4.418
Rodwell 4.317
Grimsley 4.19
Solomon 4.482
Lehninger 4.431
Nozaki 4.584
DTASelect 4.863
Thurlkill 4.329
EMBOSS 4.431
Sillero 4.622
Patrickios 3.312
IPC_peptide 4.482
IPC2_peptide 4.596
IPC2.peptide.svr19 4.577
Protein with the highest isoelectric point:
>tr|A0A139ZPK3|A0A139ZPK3_9CAUD HTH cro/C1-type domain-containing protein OS=Marinitoga camini virus 1 OX=1629954 GN=UF08_76 PE=4 SV=1
MM1 pKa = 7.55 KK2 pKa = 10.44 GGDD5 pKa = 3.54 IMAKK9 pKa = 7.17 TNKK12 pKa = 9.12 GKK14 pKa = 10.7 KK15 pKa = 8.38 IVPVKK20 pKa = 10.69 SYY22 pKa = 8.34 TRR24 pKa = 11.84 KK25 pKa = 10.19 KK26 pKa = 9.22 NGKK29 pKa = 7.72 IEE31 pKa = 3.98 KK32 pKa = 9.69 VRR34 pKa = 11.84 GHH36 pKa = 6.44 RR37 pKa = 11.84 RR38 pKa = 11.84 STPNN42 pKa = 2.98
Molecular weight: 4.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.216
IPC2_protein 10.204
IPC_protein 11.023
Toseland 11.462
ProMoST 11.389
Dawson 11.491
Bjellqvist 11.242
Wikipedia 11.754
Rodwell 11.901
Grimsley 11.52
Solomon 11.725
Lehninger 11.681
Nozaki 11.433
DTASelect 11.242
Thurlkill 11.433
EMBOSS 11.886
Sillero 11.447
Patrickios 11.637
IPC_peptide 11.74
IPC2_peptide 10.189
IPC2.peptide.svr19 8.367
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
77
0
77
15943
41
1373
207.1
23.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.754 ± 0.417
0.452 ± 0.075
5.551 ± 0.258
8.342 ± 0.454
4.88 ± 0.243
5.068 ± 0.262
1.305 ± 0.135
10.287 ± 0.279
11.008 ± 0.545
8.882 ± 0.265
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.007 ± 0.117
7.138 ± 0.254
2.716 ± 0.174
3.023 ± 0.246
3.318 ± 0.283
5.52 ± 0.363
5.231 ± 0.423
4.93 ± 0.229
1.123 ± 0.137
4.466 ± 0.225
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here