Ornithinibacillus sp. L9

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Ornithinibacillus

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4042 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6N8FHT5|A0A6N8FHT5_9BACI Uncharacterized protein OS=Ornithinibacillus sp. L9 OX=2678566 GN=GMD78_12280 PE=4 SV=1
MM1 pKa = 7.18NKK3 pKa = 9.86YY4 pKa = 9.92YY5 pKa = 10.47KK6 pKa = 10.4LAMIILFSGLLLIGCGSDD24 pKa = 4.81DD25 pKa = 5.37DD26 pKa = 4.93AAEE29 pKa = 4.35PNQGDD34 pKa = 4.28SYY36 pKa = 11.27PVNNKK41 pKa = 10.05DD42 pKa = 3.6EE43 pKa = 4.59SDD45 pKa = 3.82SNAEE49 pKa = 4.09NDD51 pKa = 3.79SEE53 pKa = 5.05SDD55 pKa = 3.54VSGSKK60 pKa = 8.27VTEE63 pKa = 3.5IHH65 pKa = 5.74TLEE68 pKa = 4.49EE69 pKa = 4.01EE70 pKa = 4.55DD71 pKa = 5.72DD72 pKa = 3.53ITRR75 pKa = 11.84EE76 pKa = 4.11NISINYY82 pKa = 8.72DD83 pKa = 3.15GSVVYY88 pKa = 10.24FDD90 pKa = 5.52LVDD93 pKa = 4.63DD94 pKa = 4.42SGEE97 pKa = 4.41DD98 pKa = 3.67EE99 pKa = 4.49TNTPYY104 pKa = 10.42FYY106 pKa = 11.32YY107 pKa = 10.72NGDD110 pKa = 3.54MFDD113 pKa = 3.75ASSVDD118 pKa = 3.46SFSDD122 pKa = 3.16CKK124 pKa = 11.33YY125 pKa = 10.65NVNAADD131 pKa = 4.04TIYY134 pKa = 9.3FTGTCKK140 pKa = 10.5DD141 pKa = 3.56EE142 pKa = 4.47KK143 pKa = 9.65DD144 pKa = 3.46TRR146 pKa = 11.84VSVVYY151 pKa = 10.51DD152 pKa = 3.1ITEE155 pKa = 3.85NTITHH160 pKa = 5.23STDD163 pKa = 2.93RR164 pKa = 11.84DD165 pKa = 3.82RR166 pKa = 11.84NVHH169 pKa = 5.13VLNDD173 pKa = 3.34GRR175 pKa = 11.84VLYY178 pKa = 8.11TTEE181 pKa = 3.99FSEE184 pKa = 5.67DD185 pKa = 3.02GTIYY189 pKa = 10.8EE190 pKa = 4.12LTEE193 pKa = 4.71DD194 pKa = 3.89GEE196 pKa = 4.65EE197 pKa = 3.95PFIEE201 pKa = 5.3IEE203 pKa = 4.76DD204 pKa = 3.86MPTILNFSVDD214 pKa = 3.47QNNEE218 pKa = 3.35VFFIYY223 pKa = 10.09GANDD227 pKa = 3.9EE228 pKa = 4.48YY229 pKa = 10.66WNSYY233 pKa = 9.5VFDD236 pKa = 3.91TTSDD240 pKa = 3.74SEE242 pKa = 4.31PVPFQSVPDD251 pKa = 4.3DD252 pKa = 4.63DD253 pKa = 4.57EE254 pKa = 4.37ATTVEE259 pKa = 4.63GRR261 pKa = 11.84ISPDD265 pKa = 2.45GKK267 pKa = 10.17YY268 pKa = 10.49IMYY271 pKa = 9.67RR272 pKa = 11.84YY273 pKa = 10.0RR274 pKa = 11.84GVTGGKK280 pKa = 9.15HH281 pKa = 4.08YY282 pKa = 11.02NYY284 pKa = 9.99VDD286 pKa = 3.31SYY288 pKa = 11.16IYY290 pKa = 10.82NRR292 pKa = 11.84DD293 pKa = 3.51SEE295 pKa = 4.68EE296 pKa = 4.25EE297 pKa = 3.63IHH299 pKa = 6.65LGHH302 pKa = 6.55GFDD305 pKa = 3.84MNFVRR310 pKa = 11.84SNGFVFDD317 pKa = 3.73EE318 pKa = 4.07VRR320 pKa = 11.84EE321 pKa = 4.06YY322 pKa = 11.44GQVIYY327 pKa = 11.25NMEE330 pKa = 4.37TDD332 pKa = 2.93TWLAPDD338 pKa = 4.16TADD341 pKa = 2.71ITYY344 pKa = 10.26FDD346 pKa = 3.54STYY349 pKa = 10.66KK350 pKa = 10.72DD351 pKa = 3.55EE352 pKa = 4.93YY353 pKa = 10.95LDD355 pKa = 4.3EE356 pKa = 5.89ADD358 pKa = 4.31QEE360 pKa = 4.37DD361 pKa = 4.3FQRR364 pKa = 11.84SSFVAISGDD373 pKa = 3.54GEE375 pKa = 4.66TVLTLDD381 pKa = 3.83RR382 pKa = 11.84FRR384 pKa = 11.84PLSEE388 pKa = 4.65DD389 pKa = 2.89RR390 pKa = 11.84VEE392 pKa = 3.89YY393 pKa = 10.93SKK395 pKa = 11.38LHH397 pKa = 5.98TISTEE402 pKa = 3.95DD403 pKa = 3.45YY404 pKa = 9.86IKK406 pKa = 10.64FLEE409 pKa = 4.82EE410 pKa = 3.6YY411 pKa = 10.21DD412 pKa = 3.65IEE414 pKa = 5.0IDD416 pKa = 4.18FEE418 pKa = 4.37PSPFDD423 pKa = 3.5EE424 pKa = 5.29KK425 pKa = 11.35GAA427 pKa = 3.85

Molecular weight: 48.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6N8FEN4|A0A6N8FEN4_9BACI Hemolysin III family protein OS=Ornithinibacillus sp. L9 OX=2678566 GN=GMD78_06170 PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 8.12KK14 pKa = 8.46VHH16 pKa = 5.5GFRR19 pKa = 11.84TRR21 pKa = 11.84MSTKK25 pKa = 10.06NGRR28 pKa = 11.84KK29 pKa = 8.49VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4042

0

4042

1157730

10

3029

286.4

32.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.318 ± 0.039

0.623 ± 0.01

5.482 ± 0.034

7.813 ± 0.05

4.547 ± 0.031

6.66 ± 0.041

2.147 ± 0.021

8.302 ± 0.041

6.653 ± 0.041

9.503 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.746 ± 0.019

4.822 ± 0.03

3.52 ± 0.022

3.737 ± 0.024

3.865 ± 0.03

5.994 ± 0.031

5.481 ± 0.029

7.027 ± 0.034

1.043 ± 0.017

3.716 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski