Actinobacteria bacterium

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; unclassified Actinomycetia

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3711 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6L5G0D3|A0A6L5G0D3_9ACTN DksA C4-type domain-containing protein OS=Actinobacteria bacterium OX=1883427 GN=GEU71_13755 PE=4 SV=1
MM1 pKa = 7.06ITLFNEE7 pKa = 4.56SSQAEE12 pKa = 4.22PPICADD18 pKa = 4.24GLDD21 pKa = 3.89EE22 pKa = 4.5TQLEE26 pKa = 4.51AIIAQPNDD34 pKa = 3.33YY35 pKa = 10.42YY36 pKa = 11.66LEE38 pKa = 4.52IGNEE42 pKa = 4.0DD43 pKa = 2.83TGGFLKK49 pKa = 10.73SQLWAEE55 pKa = 4.33GDD57 pKa = 3.71APDD60 pKa = 5.29PSEE63 pKa = 4.87GCDD66 pKa = 3.26NSDD69 pKa = 3.08SRR71 pKa = 11.84SYY73 pKa = 11.78GDD75 pKa = 4.51LPIGTWLRR83 pKa = 11.84EE84 pKa = 4.0LLLSEE89 pKa = 5.36GSPQGDD95 pKa = 3.67PLEE98 pKa = 4.81SSDD101 pKa = 3.56IQDD104 pKa = 3.39VGSSLQLKK112 pKa = 9.9GSTSGPPMWMYY123 pKa = 9.31VTAQNPADD131 pKa = 3.79LSKK134 pKa = 11.54DD135 pKa = 3.07EE136 pKa = 4.98GIRR139 pKa = 11.84TEE141 pKa = 4.11HH142 pKa = 6.34VSVIRR147 pKa = 11.84GFEE150 pKa = 3.95ITHH153 pKa = 5.47LTVANGSFDD162 pKa = 3.95GYY164 pKa = 10.7NASNGQWEE172 pKa = 4.45LSIIAYY178 pKa = 9.87GGAEE182 pKa = 4.8GITPKK187 pKa = 10.64LSSDD191 pKa = 3.42QTLDD195 pKa = 3.01WFDD198 pKa = 3.54AALGYY203 pKa = 10.86ADD205 pKa = 4.26TSPPPSCAA213 pKa = 3.3

Molecular weight: 22.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6L5G1D7|A0A6L5G1D7_9ACTN NAD(P)-binding protein OS=Actinobacteria bacterium OX=1883427 GN=GEU71_12515 PE=4 SV=1
MM1 pKa = 7.44GSLVKK6 pKa = 10.25KK7 pKa = 9.81RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.63KK11 pKa = 9.26MRR13 pKa = 11.84KK14 pKa = 8.0KK15 pKa = 10.11KK16 pKa = 9.19HH17 pKa = 5.69RR18 pKa = 11.84KK19 pKa = 6.5MLKK22 pKa = 6.94KK23 pKa = 9.13TRR25 pKa = 11.84WQRR28 pKa = 11.84RR29 pKa = 11.84QQGKK33 pKa = 9.75

Molecular weight: 4.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3711

0

3711

1073929

25

1737

289.4

31.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.077 ± 0.057

0.796 ± 0.013

6.251 ± 0.042

6.872 ± 0.04

3.487 ± 0.028

9.233 ± 0.043

2.026 ± 0.02

5.013 ± 0.023

2.961 ± 0.032

9.816 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.168 ± 0.017

2.26 ± 0.022

5.252 ± 0.03

2.511 ± 0.022

7.063 ± 0.049

5.857 ± 0.027

5.553 ± 0.027

8.282 ± 0.03

1.358 ± 0.019

2.162 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski