Amino acid dipepetide frequency for Actinobacteria bacterium

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	14.147AlaAla: 14.147 ± 0.157	0.945AlaCys: 0.945 ± 0.027	6.128AlaAsp: 6.128 ± 0.085	7.536AlaGlu: 7.536 ± 0.082	3.836AlaPhe: 3.836 ± 0.06	10.582AlaGly: 10.582 ± 0.132	2.025AlaHis: 2.025 ± 0.042	5.731AlaIle: 5.731 ± 0.081	3.079AlaLys: 3.079 ± 0.067	11.768AlaLeu: 11.768 ± 0.123	2.612AlaMet: 2.612 ± 0.052	2.211AlaAsn: 2.211 ± 0.049	4.8AlaPro: 4.8 ± 0.079	2.773AlaGln: 2.773 ± 0.057	7.904AlaArg: 7.904 ± 0.104	6.195AlaSer: 6.195 ± 0.068	6.194AlaThr: 6.194 ± 0.081	8.655AlaVal: 8.655 ± 0.109	1.426AlaTrp: 1.426 ± 0.039	2.226AlaTyr: 2.226 ± 0.048	0.0AlaXaa: 0.0 ± 0.0
Cys	0.742CysAla: 0.742 ± 0.027	0.109CysCys: 0.109 ± 0.01	0.524CysAsp: 0.524 ± 0.023	0.502CysGlu: 0.502 ± 0.018	0.291CysPhe: 0.291 ± 0.017	0.898CysGly: 0.898 ± 0.033	0.218CysHis: 0.218 ± 0.015	0.312CysIle: 0.312 ± 0.018	0.201CysLys: 0.201 ± 0.013	0.675CysLeu: 0.675 ± 0.026	0.133CysMet: 0.133 ± 0.011	0.202CysAsn: 0.202 ± 0.014	0.515CysPro: 0.515 ± 0.024	0.161CysGln: 0.161 ± 0.013	0.609CysArg: 0.609 ± 0.024	0.548CysSer: 0.548 ± 0.022	0.474CysThr: 0.474 ± 0.021	0.52CysVal: 0.52 ± 0.024	0.112CysTrp: 0.112 ± 0.011	0.22CysTyr: 0.22 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	6.746AspAla: 6.746 ± 0.079	0.448AspCys: 0.448 ± 0.022	4.24AspAsp: 4.24 ± 0.068	4.935AspGlu: 4.935 ± 0.066	2.05AspPhe: 2.05 ± 0.044	5.837AspGly: 5.837 ± 0.092	1.449AspHis: 1.449 ± 0.037	2.837AspIle: 2.837 ± 0.059	1.379AspLys: 1.379 ± 0.038	6.865AspLeu: 6.865 ± 0.088	1.035AspMet: 1.035 ± 0.035	1.175AspAsn: 1.175 ± 0.04	4.382AspPro: 4.382 ± 0.067	1.652AspGln: 1.652 ± 0.04	4.629AspArg: 4.629 ± 0.077	2.835AspSer: 2.835 ± 0.049	2.57AspThr: 2.57 ± 0.055	5.196AspVal: 5.196 ± 0.073	0.827AspTrp: 0.827 ± 0.032	1.417AspTyr: 1.417 ± 0.037	0.0AspXaa: 0.0 ± 0.0
Glu	7.693GluAla: 7.693 ± 0.107	0.399GluCys: 0.399 ± 0.019	4.0GluAsp: 4.0 ± 0.063	4.822GluGlu: 4.822 ± 0.077	2.092GluPhe: 2.092 ± 0.05	5.902GluGly: 5.902 ± 0.07	1.47GluHis: 1.47 ± 0.036	4.094GluIle: 4.094 ± 0.066	2.012GluLys: 2.012 ± 0.054	6.836GluLeu: 6.836 ± 0.093	1.67GluMet: 1.67 ± 0.041	1.397GluAsn: 1.397 ± 0.037	3.297GluPro: 3.297 ± 0.056	2.038GluGln: 2.038 ± 0.048	5.945GluArg: 5.945 ± 0.085	3.324GluSer: 3.324 ± 0.06	3.489GluThr: 3.489 ± 0.055	6.128GluVal: 6.128 ± 0.089	0.859GluTrp: 0.859 ± 0.031	1.247GluTyr: 1.247 ± 0.035	0.0GluXaa: 0.0 ± 0.0
Phe	3.658PheAla: 3.658 ± 0.063	0.342PheCys: 0.342 ± 0.017	2.509PheAsp: 2.509 ± 0.059	2.523PheGlu: 2.523 ± 0.049	1.377PhePhe: 1.377 ± 0.042	3.528PheGly: 3.528 ± 0.065	0.715PheHis: 0.715 ± 0.029	1.522PheIle: 1.522 ± 0.042	0.901PheLys: 0.901 ± 0.029	3.288PheLeu: 3.288 ± 0.07	0.629PheMet: 0.629 ± 0.022	0.818PheAsn: 0.818 ± 0.029	1.685PhePro: 1.685 ± 0.043	0.852PheGln: 0.852 ± 0.029	2.106PheArg: 2.106 ± 0.047	2.195PheSer: 2.195 ± 0.045	1.946PheThr: 1.946 ± 0.047	3.034PheVal: 3.034 ± 0.053	0.456PheTrp: 0.456 ± 0.019	0.783PheTyr: 0.783 ± 0.028	0.0PheXaa: 0.0 ± 0.0
Gly	9.613GlyAla: 9.613 ± 0.099	0.83GlyCys: 0.83 ± 0.03	5.788GlyAsp: 5.788 ± 0.093	5.606GlyGlu: 5.606 ± 0.072	3.548GlyPhe: 3.548 ± 0.056	8.634GlyGly: 8.634 ± 0.139	1.934GlyHis: 1.934 ± 0.04	4.966GlyIle: 4.966 ± 0.079	2.957GlyLys: 2.957 ± 0.06	8.781GlyLeu: 8.781 ± 0.106	2.268GlyMet: 2.268 ± 0.043	2.202GlyAsn: 2.202 ± 0.068	4.167GlyPro: 4.167 ± 0.072	2.106GlyGln: 2.106 ± 0.053	6.336GlyArg: 6.336 ± 0.079	5.908GlySer: 5.908 ± 0.085	5.546GlyThr: 5.546 ± 0.087	7.356GlyVal: 7.356 ± 0.099	1.455GlyTrp: 1.455 ± 0.042	2.333GlyTyr: 2.333 ± 0.058	0.0GlyXaa: 0.0 ± 0.0
His	1.913HisAla: 1.913 ± 0.044	0.184HisCys: 0.184 ± 0.014	1.281HisAsp: 1.281 ± 0.037	1.292HisGlu: 1.292 ± 0.038	0.668HisPhe: 0.668 ± 0.025	1.916HisGly: 1.916 ± 0.049	0.607HisHis: 0.607 ± 0.029	0.912HisIle: 0.912 ± 0.028	0.423HisLys: 0.423 ± 0.022	2.232HisLeu: 2.232 ± 0.045	0.381HisMet: 0.381 ± 0.02	0.467HisAsn: 0.467 ± 0.02	1.576HisPro: 1.576 ± 0.041	0.533HisGln: 0.533 ± 0.025	1.519HisArg: 1.519 ± 0.039	1.12HisSer: 1.12 ± 0.035	0.89HisThr: 0.89 ± 0.031	1.551HisVal: 1.551 ± 0.039	0.277HisTrp: 0.277 ± 0.017	0.522HisTyr: 0.522 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	6.342IleAla: 6.342 ± 0.083	0.438IleCys: 0.438 ± 0.019	3.77IleAsp: 3.77 ± 0.066	4.332IleGlu: 4.332 ± 0.064	1.576IlePhe: 1.576 ± 0.04	4.771IleGly: 4.771 ± 0.07	0.935IleHis: 0.935 ± 0.029	1.779IleIle: 1.779 ± 0.047	1.386IleLys: 1.386 ± 0.038	4.28IleLeu: 4.28 ± 0.077	0.722IleMet: 0.722 ± 0.027	1.14IleAsn: 1.14 ± 0.032	2.702IlePro: 2.702 ± 0.06	1.17IleGln: 1.17 ± 0.031	3.114IleArg: 3.114 ± 0.054	2.846IleSer: 2.846 ± 0.049	2.699IleThr: 2.699 ± 0.053	4.559IleVal: 4.559 ± 0.071	0.549IleTrp: 0.549 ± 0.026	1.027IleTyr: 1.027 ± 0.031	0.0IleXaa: 0.0 ± 0.0
Lys	3.175LysAla: 3.175 ± 0.072	0.162LysCys: 0.162 ± 0.013	1.738LysAsp: 1.738 ± 0.046	2.011LysGlu: 2.011 ± 0.048	0.675LysPhe: 0.675 ± 0.027	2.632LysGly: 2.632 ± 0.062	0.611LysHis: 0.611 ± 0.022	1.485LysIle: 1.485 ± 0.045	1.43LysLys: 1.43 ± 0.067	2.793LysLeu: 2.793 ± 0.05	0.632LysMet: 0.632 ± 0.025	0.765LysAsn: 0.765 ± 0.032	1.522LysPro: 1.522 ± 0.037	0.814LysGln: 0.814 ± 0.029	2.282LysArg: 2.282 ± 0.051	1.595LysSer: 1.595 ± 0.044	1.786LysThr: 1.786 ± 0.049	2.595LysVal: 2.595 ± 0.049	0.372LysTrp: 0.372 ± 0.016	0.534LysTyr: 0.534 ± 0.025	0.0LysXaa: 0.0 ± 0.0
Leu	11.867LeuAla: 11.867 ± 0.143	0.738LeuCys: 0.738 ± 0.028	6.472LeuAsp: 6.472 ± 0.084	6.965LeuGlu: 6.965 ± 0.075	3.259LeuPhe: 3.259 ± 0.069	9.285LeuGly: 9.285 ± 0.113	1.767LeuHis: 1.767 ± 0.042	4.797LeuIle: 4.797 ± 0.086	3.052LeuLys: 3.052 ± 0.057	9.377LeuLeu: 9.377 ± 0.118	2.054LeuMet: 2.054 ± 0.047	2.102LeuAsn: 2.102 ± 0.048	4.864LeuPro: 4.864 ± 0.067	2.326LeuGln: 2.326 ± 0.046	6.592LeuArg: 6.592 ± 0.08	5.871LeuSer: 5.871 ± 0.075	5.399LeuThr: 5.399 ± 0.068	8.332LeuVal: 8.332 ± 0.096	1.113LeuTrp: 1.113 ± 0.033	1.927LeuTyr: 1.927 ± 0.047	0.0LeuXaa: 0.0 ± 0.0
Met	2.43MetAla: 2.43 ± 0.044	0.132MetCys: 0.132 ± 0.012	1.187MetAsp: 1.187 ± 0.033	1.17MetGlu: 1.17 ± 0.032	0.701MetPhe: 0.701 ± 0.023	1.805MetGly: 1.805 ± 0.041	0.411MetHis: 0.411 ± 0.019	1.097MetIle: 1.097 ± 0.032	0.873MetLys: 0.873 ± 0.027	1.981MetLeu: 1.981 ± 0.043	0.474MetMet: 0.474 ± 0.021	0.643MetAsn: 0.643 ± 0.023	1.179MetPro: 1.179 ± 0.032	0.548MetGln: 0.548 ± 0.025	1.591MetArg: 1.591 ± 0.037	1.514MetSer: 1.514 ± 0.033	1.619MetThr: 1.619 ± 0.044	1.696MetVal: 1.696 ± 0.041	0.27MetTrp: 0.27 ± 0.016	0.362MetTyr: 0.362 ± 0.019	0.0MetXaa: 0.0 ± 0.0
Asn	2.359AsnAla: 2.359 ± 0.049	0.203AsnCys: 0.203 ± 0.016	1.533AsnAsp: 1.533 ± 0.053	1.335AsnGlu: 1.335 ± 0.032	0.843AsnPhe: 0.843 ± 0.028	2.07AsnGly: 2.07 ± 0.051	0.493AsnHis: 0.493 ± 0.022	1.037AsnIle: 1.037 ± 0.033	0.58AsnLys: 0.58 ± 0.027	2.345AsnLeu: 2.345 ± 0.048	0.4AsnMet: 0.4 ± 0.019	0.586AsnAsn: 0.586 ± 0.031	1.643AsnPro: 1.643 ± 0.042	0.625AsnGln: 0.625 ± 0.025	1.483AsnArg: 1.483 ± 0.04	1.119AsnSer: 1.119 ± 0.035	1.089AsnThr: 1.089 ± 0.033	1.951AsnVal: 1.951 ± 0.049	0.312AsnTrp: 0.312 ± 0.018	0.593AsnTyr: 0.593 ± 0.026	0.0AsnXaa: 0.0 ± 0.0
Pro	5.266ProAla: 5.266 ± 0.077	0.307ProCys: 0.307 ± 0.018	3.915ProAsp: 3.915 ± 0.061	4.536ProGlu: 4.536 ± 0.08	1.823ProPhe: 1.823 ± 0.044	5.191ProGly: 5.191 ± 0.077	1.045ProHis: 1.045 ± 0.03	2.269ProIle: 2.269 ± 0.047	1.645ProLys: 1.645 ± 0.045	4.457ProLeu: 4.457 ± 0.058	1.138ProMet: 1.138 ± 0.035	1.245ProAsn: 1.245 ± 0.038	2.922ProPro: 2.922 ± 0.064	1.306ProGln: 1.306 ± 0.036	3.132ProArg: 3.132 ± 0.058	3.31ProSer: 3.31 ± 0.061	2.913ProThr: 2.913 ± 0.056	4.208ProVal: 4.208 ± 0.052	0.732ProTrp: 0.732 ± 0.027	1.161ProTyr: 1.161 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	2.816GlnAla: 2.816 ± 0.059	0.162GlnCys: 0.162 ± 0.013	1.23GlnAsp: 1.23 ± 0.035	1.609GlnGlu: 1.609 ± 0.041	0.715GlnPhe: 0.715 ± 0.027	2.051GlnGly: 2.051 ± 0.047	0.493GlnHis: 0.493 ± 0.023	1.539GlnIle: 1.539 ± 0.033	0.845GlnLys: 0.845 ± 0.028	2.468GlnLeu: 2.468 ± 0.047	0.649GlnMet: 0.649 ± 0.023	0.619GlnAsn: 0.619 ± 0.018	1.288GlnPro: 1.288 ± 0.041	0.867GlnGln: 0.867 ± 0.034	2.067GlnArg: 2.067 ± 0.044	1.311GlnSer: 1.311 ± 0.034	1.428GlnThr: 1.428 ± 0.036	2.154GlnVal: 2.154 ± 0.042	0.342GlnTrp: 0.342 ± 0.018	0.46GlnTyr: 0.46 ± 0.018	0.0GlnXaa: 0.0 ± 0.0
Arg	7.287ArgAla: 7.287 ± 0.097	0.602ArgCys: 0.602 ± 0.027	3.99ArgAsp: 3.99 ± 0.057	4.257ArgGlu: 4.257 ± 0.068	2.82ArgPhe: 2.82 ± 0.059	5.282ArgGly: 5.282 ± 0.082	1.468ArgHis: 1.468 ± 0.041	3.852ArgIle: 3.852 ± 0.063	2.365ArgLys: 2.365 ± 0.063	7.311ArgLeu: 7.311 ± 0.088	1.803ArgMet: 1.803 ± 0.04	1.604ArgAsn: 1.604 ± 0.037	3.444ArgPro: 3.444 ± 0.062	1.734ArgGln: 1.734 ± 0.04	5.989ArgArg: 5.989 ± 0.095	4.775ArgSer: 4.775 ± 0.075	3.757ArgThr: 3.757 ± 0.056	5.262ArgVal: 5.262 ± 0.067	1.215ArgTrp: 1.215 ± 0.032	1.808ArgTyr: 1.808 ± 0.048	0.0ArgXaa: 0.0 ± 0.0
Ser	5.843SerAla: 5.843 ± 0.074	0.457SerCys: 0.457 ± 0.019	3.501SerAsp: 3.501 ± 0.059	3.748SerGlu: 3.748 ± 0.053	2.209SerPhe: 2.209 ± 0.047	6.142SerGly: 6.142 ± 0.081	1.128SerHis: 1.128 ± 0.029	3.01SerIle: 3.01 ± 0.045	1.63SerLys: 1.63 ± 0.037	5.807SerLeu: 5.807 ± 0.077	1.509SerMet: 1.509 ± 0.033	1.353SerAsn: 1.353 ± 0.038	3.199SerPro: 3.199 ± 0.061	1.448SerGln: 1.448 ± 0.036	3.833SerArg: 3.833 ± 0.065	3.803SerSer: 3.803 ± 0.063	3.275SerThr: 3.275 ± 0.066	4.492SerVal: 4.492 ± 0.071	0.872SerTrp: 0.872 ± 0.026	1.317SerTyr: 1.317 ± 0.034	0.0SerXaa: 0.0 ± 0.0
Thr	5.756ThrAla: 5.756 ± 0.079	0.467ThrCys: 0.467 ± 0.025	3.29ThrAsp: 3.29 ± 0.06	3.57ThrGlu: 3.57 ± 0.063	2.159ThrPhe: 2.159 ± 0.041	5.629ThrGly: 5.629 ± 0.08	1.125ThrHis: 1.125 ± 0.034	3.01ThrIle: 3.01 ± 0.054	1.579ThrLys: 1.579 ± 0.041	5.092ThrLeu: 5.092 ± 0.075	1.169ThrMet: 1.169 ± 0.033	1.292ThrAsn: 1.292 ± 0.034	3.294ThrPro: 3.294 ± 0.066	1.359ThrGln: 1.359 ± 0.036	3.339ThrArg: 3.339 ± 0.052	3.366ThrSer: 3.366 ± 0.055	3.363ThrThr: 3.363 ± 0.068	4.541ThrVal: 4.541 ± 0.071	0.794ThrTrp: 0.794 ± 0.035	1.34ThrTyr: 1.34 ± 0.034	0.0ThrXaa: 0.0 ± 0.0
Val	9.794ValAla: 9.794 ± 0.112	0.712ValCys: 0.712 ± 0.027	5.259ValAsp: 5.259 ± 0.065	5.621ValGlu: 5.621 ± 0.088	2.828ValPhe: 2.828 ± 0.049	6.901ValGly: 6.901 ± 0.104	1.656ValHis: 1.656 ± 0.043	4.339ValIle: 4.339 ± 0.067	2.288ValLys: 2.288 ± 0.044	8.131ValLeu: 8.131 ± 0.104	1.683ValMet: 1.683 ± 0.039	1.894ValAsn: 1.894 ± 0.041	4.311ValPro: 4.311 ± 0.066	1.777ValGln: 1.777 ± 0.037	5.344ValArg: 5.344 ± 0.072	4.724ValSer: 4.724 ± 0.066	5.177ValThr: 5.177 ± 0.069	7.852ValVal: 7.852 ± 0.096	0.988ValTrp: 0.988 ± 0.036	1.541ValTyr: 1.541 ± 0.036	0.0ValXaa: 0.0 ± 0.0
Trp	1.297TrpAla: 1.297 ± 0.033	0.128TrpCys: 0.128 ± 0.01	0.837TrpAsp: 0.837 ± 0.029	0.796TrpGlu: 0.796 ± 0.033	0.569TrpPhe: 0.569 ± 0.025	1.146TrpGly: 1.146 ± 0.035	0.313TrpHis: 0.313 ± 0.015	0.723TrpIle: 0.723 ± 0.023	0.42TrpLys: 0.42 ± 0.021	1.341TrpLeu: 1.341 ± 0.037	0.358TrpMet: 0.358 ± 0.017	0.384TrpAsn: 0.384 ± 0.021	0.557TrpPro: 0.557 ± 0.021	0.396TrpGln: 0.396 ± 0.018	1.006TrpArg: 1.006 ± 0.032	0.926TrpSer: 0.926 ± 0.039	0.849TrpThr: 0.849 ± 0.034	0.99TrpVal: 0.99 ± 0.032	0.267TrpTrp: 0.267 ± 0.018	0.274TrpTyr: 0.274 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.029TyrAla: 2.029 ± 0.048	0.199TyrCys: 0.199 ± 0.013	1.313TyrAsp: 1.313 ± 0.038	1.55TyrGlu: 1.55 ± 0.037	0.829TyrPhe: 0.829 ± 0.03	2.131TyrGly: 2.131 ± 0.046	0.403TyrHis: 0.403 ± 0.019	0.823TyrIle: 0.823 ± 0.031	0.562TyrLys: 0.562 ± 0.024	2.333TyrLeu: 2.333 ± 0.048	0.364TyrMet: 0.364 ± 0.02	0.499TyrAsn: 0.499 ± 0.025	1.174TyrPro: 1.174 ± 0.035	0.629TyrGln: 0.629 ± 0.026	1.809TyrArg: 1.809 ± 0.049	1.289TyrSer: 1.289 ± 0.031	1.069TyrThr: 1.069 ± 0.033	1.749TyrVal: 1.749 ± 0.042	0.336TyrTrp: 0.336 ± 0.021	0.532TyrTyr: 0.532 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3711 proteins (1073929 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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