Enterobacteria phage GA (Bacteriophage GA)

Taxonomy: Viruses; Riboviria; Orthornavirae; Lenarviricota; Leviviricetes; Norzivirales; Fiersviridae; Emesvirus; Emesvirus japonicum

Average proteome isoelectric point is 8.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P07394|MATA_BPGA Maturation protein A OS=Enterobacteria phage GA OX=12018 GN=A PE=2 SV=1
MM1 pKa = 7.12FRR3 pKa = 11.84FRR5 pKa = 11.84EE6 pKa = 4.05IEE8 pKa = 3.77KK9 pKa = 8.89TLCMDD14 pKa = 4.01RR15 pKa = 11.84TRR17 pKa = 11.84DD18 pKa = 3.47CAVRR22 pKa = 11.84FHH24 pKa = 7.27VYY26 pKa = 9.84LQSLDD31 pKa = 4.29LGSSDD36 pKa = 4.25PLSPDD41 pKa = 3.21FDD43 pKa = 3.73GLAYY47 pKa = 10.7LRR49 pKa = 11.84DD50 pKa = 3.68EE51 pKa = 5.36CLTKK55 pKa = 10.59HH56 pKa = 6.39PSLGDD61 pKa = 3.7SNSDD65 pKa = 3.12ARR67 pKa = 11.84RR68 pKa = 11.84KK69 pKa = 9.05EE70 pKa = 3.55LAYY73 pKa = 10.51AKK75 pKa = 10.79LMDD78 pKa = 4.09SDD80 pKa = 4.22QRR82 pKa = 11.84CKK84 pKa = 10.15IQNSNGYY91 pKa = 9.18DD92 pKa = 3.49YY93 pKa = 11.87SHH95 pKa = 7.23IEE97 pKa = 3.98SGVLSGILKK106 pKa = 8.4TAQALVANLLTGFEE120 pKa = 4.52SHH122 pKa = 7.27FLNDD126 pKa = 3.53CSFSNGASQGFKK138 pKa = 10.78LRR140 pKa = 11.84DD141 pKa = 3.12AAPFKK146 pKa = 10.75KK147 pKa = 9.93IAGQATVTAPAYY159 pKa = 9.74DD160 pKa = 3.34IAVAAVKK167 pKa = 9.25TCAPWYY173 pKa = 10.42AYY175 pKa = 8.05MQEE178 pKa = 4.3TYY180 pKa = 11.25GDD182 pKa = 3.41EE183 pKa = 4.55TKK185 pKa = 10.08WFRR188 pKa = 11.84RR189 pKa = 11.84VYY191 pKa = 10.56GNGLFSVPKK200 pKa = 9.87NNKK203 pKa = 8.33IDD205 pKa = 3.54RR206 pKa = 11.84AACKK210 pKa = 10.1EE211 pKa = 3.91PDD213 pKa = 3.19MNMYY217 pKa = 9.01LQKK220 pKa = 10.87GAGSFIRR227 pKa = 11.84KK228 pKa = 9.06RR229 pKa = 11.84LRR231 pKa = 11.84SVGIDD236 pKa = 3.74LNDD239 pKa = 3.19QTRR242 pKa = 11.84NQEE245 pKa = 4.02LARR248 pKa = 11.84LGSIDD253 pKa = 4.18GSLATIDD260 pKa = 4.48LSSASDD266 pKa = 3.95SISDD270 pKa = 3.63RR271 pKa = 11.84LVWDD275 pKa = 4.22LLPPHH280 pKa = 6.96VYY282 pKa = 10.32SYY284 pKa = 10.91LARR287 pKa = 11.84IRR289 pKa = 11.84TSFTMIDD296 pKa = 3.38GRR298 pKa = 11.84LHH300 pKa = 5.44KK301 pKa = 9.96WGLFSTMGNGFTFEE315 pKa = 4.45LEE317 pKa = 4.66SMIFWALSKK326 pKa = 10.89SIMLSMGVTGSLGIYY341 pKa = 9.83GDD343 pKa = 4.61DD344 pKa = 3.23IIVPVEE350 pKa = 3.98CRR352 pKa = 11.84PTLLKK357 pKa = 10.79VLSAVNFLPNEE368 pKa = 4.22EE369 pKa = 4.26KK370 pKa = 10.34TFTTGYY376 pKa = 10.01FRR378 pKa = 11.84EE379 pKa = 4.32SCGAHH384 pKa = 5.96FFKK387 pKa = 11.03DD388 pKa = 3.04ADD390 pKa = 3.79MKK392 pKa = 10.79PFYY395 pKa = 10.06CKK397 pKa = 10.27RR398 pKa = 11.84PMEE401 pKa = 4.28TLPDD405 pKa = 3.68VMLLCNRR412 pKa = 11.84IRR414 pKa = 11.84GWQTVGGMSDD424 pKa = 3.34PRR426 pKa = 11.84LFPIWKK432 pKa = 8.89EE433 pKa = 3.85FADD436 pKa = 4.28MIPPKK441 pKa = 10.62FKK443 pKa = 10.78GGCNLDD449 pKa = 3.23RR450 pKa = 11.84DD451 pKa = 4.59TYY453 pKa = 11.03LVSPDD458 pKa = 3.49KK459 pKa = 11.07PGVSLVRR466 pKa = 11.84IAKK469 pKa = 9.1VRR471 pKa = 11.84SGFNHH476 pKa = 6.91AFPYY480 pKa = 9.19GHH482 pKa = 6.61EE483 pKa = 4.13NGRR486 pKa = 11.84YY487 pKa = 6.63VHH489 pKa = 6.72WLHH492 pKa = 6.32MGSGEE497 pKa = 4.1VLEE500 pKa = 5.22TISSARR506 pKa = 11.84YY507 pKa = 7.61RR508 pKa = 11.84CKK510 pKa = 10.44PNSEE514 pKa = 3.77WRR516 pKa = 11.84TQIPLFPQEE525 pKa = 5.23LEE527 pKa = 4.08ACVLSS532 pKa = 3.99

Molecular weight: 59.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P07394|MATA_BPGA Maturation protein A OS=Enterobacteria phage GA OX=12018 GN=A PE=2 SV=1
MM1 pKa = 7.25FPKK4 pKa = 10.75SNIDD8 pKa = 3.01RR9 pKa = 11.84NYY11 pKa = 9.11KK12 pKa = 9.97VKK14 pKa = 10.83LISYY18 pKa = 7.89DD19 pKa = 3.35KK20 pKa = 10.7KK21 pKa = 11.21GKK23 pKa = 9.86LVSDD27 pKa = 4.28DD28 pKa = 4.13SFEE31 pKa = 4.16QVEE34 pKa = 4.6NYY36 pKa = 10.19LFQNRR41 pKa = 11.84STTYY45 pKa = 8.33KK46 pKa = 9.31PGYY49 pKa = 8.38IRR51 pKa = 11.84RR52 pKa = 11.84DD53 pKa = 3.36FRR55 pKa = 11.84RR56 pKa = 11.84PTNFWNGYY64 pKa = 8.42RR65 pKa = 11.84CFNQPVGTFTRR76 pKa = 11.84KK77 pKa = 10.07LSDD80 pKa = 3.08GGRR83 pKa = 11.84QVADD87 pKa = 3.47YY88 pKa = 11.18GIVNPNKK95 pKa = 8.75FTANSQHH102 pKa = 6.72LGDD105 pKa = 4.56NMVIYY110 pKa = 9.42PGPFSINIDD119 pKa = 3.04QRR121 pKa = 11.84ASVEE125 pKa = 4.17VLNKK129 pKa = 10.41LSQSNLNIGVAIAEE143 pKa = 4.38AKK145 pKa = 7.55MTASLLAKK153 pKa = 10.16QSIALIRR160 pKa = 11.84AYY162 pKa = 9.19TAAKK166 pKa = 8.83RR167 pKa = 11.84GNWRR171 pKa = 11.84EE172 pKa = 3.86VLSQLLISEE181 pKa = 4.29HH182 pKa = 6.74RR183 pKa = 11.84FRR185 pKa = 11.84APAKK189 pKa = 10.09DD190 pKa = 3.66LGGRR194 pKa = 11.84WLEE197 pKa = 4.16LQYY200 pKa = 11.47GWLPLMSDD208 pKa = 3.94LKK210 pKa = 10.95AAYY213 pKa = 9.66DD214 pKa = 4.15LLTQTKK220 pKa = 9.72LPAFMPLRR228 pKa = 11.84VTRR231 pKa = 11.84TVGGTHH237 pKa = 6.26NYY239 pKa = 9.16KK240 pKa = 9.95VRR242 pKa = 11.84NVEE245 pKa = 4.16SAGDD249 pKa = 3.41TWSYY253 pKa = 9.95RR254 pKa = 11.84HH255 pKa = 6.22RR256 pKa = 11.84LSVNYY261 pKa = 9.63RR262 pKa = 11.84IWYY265 pKa = 7.99FISDD269 pKa = 4.2PRR271 pKa = 11.84LAWASSLGLLNPLEE285 pKa = 4.69IYY287 pKa = 9.32WEE289 pKa = 4.0KK290 pKa = 10.17TPWSFVVDD298 pKa = 3.47WFLPVGNLIEE308 pKa = 5.13AMSNPLGLDD317 pKa = 3.5IISGTKK323 pKa = 7.87TWQLEE328 pKa = 4.42SKK330 pKa = 10.88LNATLPASGWSGTAKK345 pKa = 9.2LTAYY349 pKa = 10.14AKK351 pKa = 10.52AYY353 pKa = 10.06DD354 pKa = 3.54RR355 pKa = 11.84STFYY359 pKa = 11.1SFPTPLPYY367 pKa = 10.52VKK369 pKa = 10.53SPLSGLHH376 pKa = 6.17LANALALINQRR387 pKa = 11.84LKK389 pKa = 10.91RR390 pKa = 3.85

Molecular weight: 44.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1115

63

532

278.8

31.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.713 ± 1.191

1.435 ± 0.743

5.471 ± 0.808

3.767 ± 0.458

4.664 ± 0.53

6.726 ± 0.494

1.614 ± 0.383

5.112 ± 0.522

5.74 ± 0.225

10.404 ± 1.075

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.422 ± 0.551

4.933 ± 0.775

4.753 ± 0.549

2.691 ± 0.328

6.099 ± 0.811

8.7 ± 0.592

5.291 ± 0.273

5.919 ± 1.637

2.332 ± 0.48

4.215 ± 0.442
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski