Porphyromonas sp. COT-108 OH1349
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1734 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A2E615|A0A0A2E615_9PORP Lipoprotein-releasing system ATP-binding protein LolD OS=Porphyromonas sp. COT-108 OH1349 OX=1537504 GN=lolD PE=3 SV=1
MM1 pKa = 7.59 EE2 pKa = 4.73 ATSLSEE8 pKa = 3.35 ARR10 pKa = 11.84 IYY12 pKa = 10.84 VGTYY16 pKa = 9.92 AKK18 pKa = 10.82 YY19 pKa = 10.22 NDD21 pKa = 3.72 GSIEE25 pKa = 4.15 GKK27 pKa = 9.58 WFDD30 pKa = 5.19 LSDD33 pKa = 4.14 FSDD36 pKa = 3.24 KK37 pKa = 11.61 DD38 pKa = 3.51 EE39 pKa = 5.1 FYY41 pKa = 10.5 EE42 pKa = 4.49 ACKK45 pKa = 10.03 EE46 pKa = 3.95 LHH48 pKa = 6.84 ADD50 pKa = 3.91 EE51 pKa = 5.33 EE52 pKa = 4.63 DD53 pKa = 3.73 PEE55 pKa = 5.1 YY56 pKa = 10.77 MFQDD60 pKa = 3.59 WEE62 pKa = 4.41 NIPDD66 pKa = 3.82 EE67 pKa = 5.08 LIGEE71 pKa = 4.38 SWLSDD76 pKa = 3.27 NFFDD80 pKa = 5.86 IRR82 pKa = 11.84 DD83 pKa = 3.79 AMDD86 pKa = 5.13 DD87 pKa = 3.63 LSEE90 pKa = 5.11 DD91 pKa = 3.58 EE92 pKa = 4.44 QQAFMVWCNHH102 pKa = 4.92 VSHH105 pKa = 7.45 DD106 pKa = 4.76 LSTEE110 pKa = 3.97 DD111 pKa = 3.42 AHH113 pKa = 8.57 DD114 pKa = 5.14 LISSFRR120 pKa = 11.84 DD121 pKa = 3.52 DD122 pKa = 4.21 FQGKK126 pKa = 9.69 YY127 pKa = 10.0 NDD129 pKa = 3.95 EE130 pKa = 3.53 EE131 pKa = 5.41 DD132 pKa = 3.57 YY133 pKa = 10.93 AYY135 pKa = 10.12 EE136 pKa = 4.12 IVEE139 pKa = 3.94 QCYY142 pKa = 9.77 EE143 pKa = 3.98 LPEE146 pKa = 3.94 FAKK149 pKa = 10.18 TYY151 pKa = 10.62 FDD153 pKa = 3.75 YY154 pKa = 11.44 EE155 pKa = 4.0 KK156 pKa = 10.43 FARR159 pKa = 11.84 DD160 pKa = 4.15 LFMGDD165 pKa = 3.13 YY166 pKa = 9.52 WFEE169 pKa = 4.16 DD170 pKa = 3.43 SFVFRR175 pKa = 11.84 ASS177 pKa = 3.02
Molecular weight: 21.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.714
IPC2_protein 3.783
IPC_protein 3.783
Toseland 3.567
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.681
Rodwell 3.605
Grimsley 3.478
Solomon 3.757
Lehninger 3.719
Nozaki 3.872
DTASelect 4.088
Thurlkill 3.617
EMBOSS 3.694
Sillero 3.897
Patrickios 0.922
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.807
Protein with the highest isoelectric point:
>tr|A0A0A2E2V3|A0A0A2E2V3_9PORP Arsenic resistance protein ArsB OS=Porphyromonas sp. COT-108 OH1349 OX=1537504 GN=JT26_00500 PE=3 SV=1
MM1 pKa = 7.65 SKK3 pKa = 9.54 ICQITGKK10 pKa = 10.27 RR11 pKa = 11.84 AMVGNNVSHH20 pKa = 6.77 SKK22 pKa = 10.45 RR23 pKa = 11.84 RR24 pKa = 11.84 TKK26 pKa = 10.52 RR27 pKa = 11.84 VFDD30 pKa = 4.24 ANLFDD35 pKa = 5.04 RR36 pKa = 11.84 KK37 pKa = 10.04 FYY39 pKa = 10.1 WVEE42 pKa = 3.35 EE43 pKa = 4.26 DD44 pKa = 2.87 CWIRR48 pKa = 11.84 IKK50 pKa = 11.04 VSAAGLRR57 pKa = 11.84 LINKK61 pKa = 9.39 IGLDD65 pKa = 3.29 AALRR69 pKa = 11.84 RR70 pKa = 11.84 AAEE73 pKa = 4.04 NGTLRR78 pKa = 11.84 KK79 pKa = 8.54 YY80 pKa = 9.25 TLLL83 pKa = 4.9
Molecular weight: 9.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.321
IPC2_protein 9.677
IPC_protein 10.204
Toseland 10.804
ProMoST 10.394
Dawson 10.877
Bjellqvist 10.526
Wikipedia 11.038
Rodwell 11.213
Grimsley 10.906
Solomon 10.979
Lehninger 10.95
Nozaki 10.789
DTASelect 10.526
Thurlkill 10.774
EMBOSS 11.184
Sillero 10.804
Patrickios 10.965
IPC_peptide 10.979
IPC2_peptide 9.458
IPC2.peptide.svr19 8.641
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1734
0
1734
596397
40
2440
343.9
38.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.147 ± 0.053
1.006 ± 0.02
5.181 ± 0.047
7.116 ± 0.059
4.519 ± 0.04
6.812 ± 0.054
2.043 ± 0.029
7.09 ± 0.05
6.741 ± 0.051
9.711 ± 0.064
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.588 ± 0.027
4.279 ± 0.045
3.949 ± 0.032
3.184 ± 0.029
5.073 ± 0.04
6.835 ± 0.051
5.287 ± 0.036
6.408 ± 0.039
1.019 ± 0.019
4.01 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here