Porphyromonas sp. COT-108 OH1349

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Porphyromonadaceae; Porphyromonas; unclassified Porphyromonas

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1734 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0A2E615|A0A0A2E615_9PORP Lipoprotein-releasing system ATP-binding protein LolD OS=Porphyromonas sp. COT-108 OH1349 OX=1537504 GN=lolD PE=3 SV=1
MM1 pKa = 7.59EE2 pKa = 4.73ATSLSEE8 pKa = 3.35ARR10 pKa = 11.84IYY12 pKa = 10.84VGTYY16 pKa = 9.92AKK18 pKa = 10.82YY19 pKa = 10.22NDD21 pKa = 3.72GSIEE25 pKa = 4.15GKK27 pKa = 9.58WFDD30 pKa = 5.19LSDD33 pKa = 4.14FSDD36 pKa = 3.24KK37 pKa = 11.61DD38 pKa = 3.51EE39 pKa = 5.1FYY41 pKa = 10.5EE42 pKa = 4.49ACKK45 pKa = 10.03EE46 pKa = 3.95LHH48 pKa = 6.84ADD50 pKa = 3.91EE51 pKa = 5.33EE52 pKa = 4.63DD53 pKa = 3.73PEE55 pKa = 5.1YY56 pKa = 10.77MFQDD60 pKa = 3.59WEE62 pKa = 4.41NIPDD66 pKa = 3.82EE67 pKa = 5.08LIGEE71 pKa = 4.38SWLSDD76 pKa = 3.27NFFDD80 pKa = 5.86IRR82 pKa = 11.84DD83 pKa = 3.79AMDD86 pKa = 5.13DD87 pKa = 3.63LSEE90 pKa = 5.11DD91 pKa = 3.58EE92 pKa = 4.44QQAFMVWCNHH102 pKa = 4.92VSHH105 pKa = 7.45DD106 pKa = 4.76LSTEE110 pKa = 3.97DD111 pKa = 3.42AHH113 pKa = 8.57DD114 pKa = 5.14LISSFRR120 pKa = 11.84DD121 pKa = 3.52DD122 pKa = 4.21FQGKK126 pKa = 9.69YY127 pKa = 10.0NDD129 pKa = 3.95EE130 pKa = 3.53EE131 pKa = 5.41DD132 pKa = 3.57YY133 pKa = 10.93AYY135 pKa = 10.12EE136 pKa = 4.12IVEE139 pKa = 3.94QCYY142 pKa = 9.77EE143 pKa = 3.98LPEE146 pKa = 3.94FAKK149 pKa = 10.18TYY151 pKa = 10.62FDD153 pKa = 3.75YY154 pKa = 11.44EE155 pKa = 4.0KK156 pKa = 10.43FARR159 pKa = 11.84DD160 pKa = 4.15LFMGDD165 pKa = 3.13YY166 pKa = 9.52WFEE169 pKa = 4.16DD170 pKa = 3.43SFVFRR175 pKa = 11.84ASS177 pKa = 3.02

Molecular weight:
21.16 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0A2E2V3|A0A0A2E2V3_9PORP Arsenic resistance protein ArsB OS=Porphyromonas sp. COT-108 OH1349 OX=1537504 GN=JT26_00500 PE=3 SV=1
MM1 pKa = 7.65SKK3 pKa = 9.54ICQITGKK10 pKa = 10.27RR11 pKa = 11.84AMVGNNVSHH20 pKa = 6.77SKK22 pKa = 10.45RR23 pKa = 11.84RR24 pKa = 11.84TKK26 pKa = 10.52RR27 pKa = 11.84VFDD30 pKa = 4.24ANLFDD35 pKa = 5.04RR36 pKa = 11.84KK37 pKa = 10.04FYY39 pKa = 10.1WVEE42 pKa = 3.35EE43 pKa = 4.26DD44 pKa = 2.87CWIRR48 pKa = 11.84IKK50 pKa = 11.04VSAAGLRR57 pKa = 11.84LINKK61 pKa = 9.39IGLDD65 pKa = 3.29AALRR69 pKa = 11.84RR70 pKa = 11.84AAEE73 pKa = 4.04NGTLRR78 pKa = 11.84KK79 pKa = 8.54YY80 pKa = 9.25TLLL83 pKa = 4.9

Molecular weight:
9.58 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1734

0

1734

596397

40

2440

343.9

38.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.147 ± 0.053

1.006 ± 0.02

5.181 ± 0.047

7.116 ± 0.059

4.519 ± 0.04

6.812 ± 0.054

2.043 ± 0.029

7.09 ± 0.05

6.741 ± 0.051

9.711 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.588 ± 0.027

4.279 ± 0.045

3.949 ± 0.032

3.184 ± 0.029

5.073 ± 0.04

6.835 ± 0.051

5.287 ± 0.036

6.408 ± 0.039

1.019 ± 0.019

4.01 ± 0.04

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski