Mycobacterium phage Kratio

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Timquatrovirus; unclassified Timquatrovirus

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 99 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5AMS6|A0A0C5AMS6_9CAUD Portal protein OS=Mycobacterium phage Kratio OX=1606763 GN=PBI_KRATIO_6 PE=4 SV=1
MM1 pKa = 7.76VEE3 pKa = 4.55HH4 pKa = 7.34LDD6 pKa = 4.31DD7 pKa = 3.98NHH9 pKa = 7.49DD10 pKa = 3.41VDD12 pKa = 4.0TDD14 pKa = 3.67HH15 pKa = 8.22AEE17 pKa = 4.24VPVSDD22 pKa = 3.66ITADD26 pKa = 4.05AITGAQQPTTTTPRR40 pKa = 11.84VKK42 pKa = 9.73GTCSNPDD49 pKa = 3.12WTPTVYY55 pKa = 10.36EE56 pKa = 4.44PYY58 pKa = 10.44RR59 pKa = 11.84PP60 pKa = 3.55

Molecular weight: 6.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5AE11|A0A0C5AE11_9CAUD HNH endonuclease OS=Mycobacterium phage Kratio OX=1606763 GN=PBI_KRATIO_100 PE=4 SV=1
MM1 pKa = 7.26NSKK4 pKa = 9.89PVRR7 pKa = 11.84KK8 pKa = 9.84VRR10 pKa = 11.84VNNGTAVHH18 pKa = 6.3VGRR21 pKa = 11.84FDD23 pKa = 3.67RR24 pKa = 11.84YY25 pKa = 8.11EE26 pKa = 4.17NKK28 pKa = 9.71VVATLCSNAVFTTEE42 pKa = 4.97NGWQTRR48 pKa = 11.84RR49 pKa = 11.84TFRR52 pKa = 11.84PAGGTVTCGRR62 pKa = 11.84CKK64 pKa = 10.28KK65 pKa = 10.41AQAKK69 pKa = 9.84LDD71 pKa = 3.76RR72 pKa = 11.84DD73 pKa = 3.62

Molecular weight: 8.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

99

0

99

19308

43

1369

195.0

21.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.948 ± 0.418

1.062 ± 0.143

6.759 ± 0.203

5.464 ± 0.287

2.615 ± 0.145

8.53 ± 0.401

2.227 ± 0.165

4.004 ± 0.174

3.48 ± 0.202

7.919 ± 0.203

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.165 ± 0.126

2.906 ± 0.192

5.386 ± 0.218

3.47 ± 0.138

7.701 ± 0.424

4.972 ± 0.241

6.578 ± 0.218

7.588 ± 0.196

2.01 ± 0.122

2.217 ± 0.146
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski