Amino acid dipepetide frequency for Mycobacterium phage Kratio

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.391AlaAla: 21.391 ± 1.694
1.191AlaCys: 1.191 ± 0.283
8.494AlaAsp: 8.494 ± 0.582
7.925AlaGlu: 7.925 ± 0.803
3.056AlaPhe: 3.056 ± 0.559
13.208AlaGly: 13.208 ± 1.017
2.227AlaHis: 2.227 ± 0.349
4.765AlaIle: 4.765 ± 0.566
3.988AlaLys: 3.988 ± 0.431
10.773AlaLeu: 10.773 ± 0.805
3.159AlaMet: 3.159 ± 0.418
3.108AlaAsn: 3.108 ± 0.463
6.164AlaPro: 6.164 ± 0.855
4.713AlaGln: 4.713 ± 0.43
8.598AlaArg: 8.598 ± 0.827
5.024AlaSer: 5.024 ± 0.562
6.63AlaThr: 6.63 ± 0.736
10.463AlaVal: 10.463 ± 0.961
2.383AlaTrp: 2.383 ± 0.37
2.227AlaTyr: 2.227 ± 0.378
0.0AlaXaa: 0.0 ± 0.0
Cys
1.191CysAla: 1.191 ± 0.265
0.052CysCys: 0.052 ± 0.064
0.57CysAsp: 0.57 ± 0.155
0.414CysGlu: 0.414 ± 0.147
0.207CysPhe: 0.207 ± 0.106
1.398CysGly: 1.398 ± 0.314
0.466CysHis: 0.466 ± 0.17
0.414CysIle: 0.414 ± 0.161
0.57CysLys: 0.57 ± 0.138
0.207CysLeu: 0.207 ± 0.099
0.311CysMet: 0.311 ± 0.148
0.259CysAsn: 0.259 ± 0.117
0.622CysPro: 0.622 ± 0.176
0.466CysGln: 0.466 ± 0.167
0.518CysArg: 0.518 ± 0.155
0.932CysSer: 0.932 ± 0.249
0.829CysThr: 0.829 ± 0.201
0.518CysVal: 0.518 ± 0.143
0.414CysTrp: 0.414 ± 0.141
0.259CysTyr: 0.259 ± 0.122
0.0CysXaa: 0.0 ± 0.0
Asp
9.064AspAla: 9.064 ± 0.81
0.932AspCys: 0.932 ± 0.266
5.438AspAsp: 5.438 ± 0.609
5.283AspGlu: 5.283 ± 0.592
1.088AspPhe: 1.088 ± 0.23
5.905AspGly: 5.905 ± 0.551
1.191AspHis: 1.191 ± 0.21
2.279AspIle: 2.279 ± 0.328
1.916AspLys: 1.916 ± 0.436
6.215AspLeu: 6.215 ± 0.459
1.554AspMet: 1.554 ± 0.31
2.02AspAsn: 2.02 ± 0.341
3.522AspPro: 3.522 ± 0.42
1.657AspGln: 1.657 ± 0.246
5.801AspArg: 5.801 ± 0.683
1.968AspSer: 1.968 ± 0.315
3.936AspThr: 3.936 ± 0.424
4.972AspVal: 4.972 ± 0.409
1.295AspTrp: 1.295 ± 0.23
1.554AspTyr: 1.554 ± 0.256
0.0AspXaa: 0.0 ± 0.0
Glu
7.407GluAla: 7.407 ± 0.807
0.57GluCys: 0.57 ± 0.153
3.108GluAsp: 3.108 ± 0.393
2.693GluGlu: 2.693 ± 0.399
1.554GluPhe: 1.554 ± 0.313
3.47GluGly: 3.47 ± 0.484
1.865GluHis: 1.865 ± 0.387
1.709GluIle: 1.709 ± 0.369
1.761GluLys: 1.761 ± 0.291
5.594GluLeu: 5.594 ± 0.586
1.502GluMet: 1.502 ± 0.225
1.139GluAsn: 1.139 ± 0.198
3.263GluPro: 3.263 ± 0.501
2.486GluGln: 2.486 ± 0.387
5.179GluArg: 5.179 ± 0.824
2.486GluSer: 2.486 ± 0.341
2.227GluThr: 2.227 ± 0.365
3.988GluVal: 3.988 ± 0.428
1.036GluTrp: 1.036 ± 0.226
1.606GluTyr: 1.606 ± 0.282
0.0GluXaa: 0.0 ± 0.0
Phe
2.642PheAla: 2.642 ± 0.421
0.259PheCys: 0.259 ± 0.109
2.434PheAsp: 2.434 ± 0.316
1.813PheGlu: 1.813 ± 0.261
0.725PhePhe: 0.725 ± 0.166
3.004PheGly: 3.004 ± 0.361
0.673PheHis: 0.673 ± 0.185
1.139PheIle: 1.139 ± 0.218
0.881PheLys: 0.881 ± 0.231
1.657PheLeu: 1.657 ± 0.28
0.673PheMet: 0.673 ± 0.169
0.881PheAsn: 0.881 ± 0.237
1.347PhePro: 1.347 ± 0.259
0.57PheGln: 0.57 ± 0.173
1.657PheArg: 1.657 ± 0.303
1.606PheSer: 1.606 ± 0.261
2.02PheThr: 2.02 ± 0.29
1.398PheVal: 1.398 ± 0.284
0.363PheTrp: 0.363 ± 0.138
0.414PheTyr: 0.414 ± 0.137
0.0PheXaa: 0.0 ± 0.0
Gly
8.909GlyAla: 8.909 ± 0.89
1.036GlyCys: 1.036 ± 0.23
6.423GlyAsp: 6.423 ± 0.568
4.403GlyGlu: 4.403 ± 0.422
2.331GlyPhe: 2.331 ± 0.417
8.443GlyGly: 8.443 ± 1.39
1.657GlyHis: 1.657 ± 0.35
3.729GlyIle: 3.729 ± 0.417
3.263GlyLys: 3.263 ± 0.44
5.594GlyLeu: 5.594 ± 0.778
2.175GlyMet: 2.175 ± 0.418
3.004GlyAsn: 3.004 ± 0.435
4.092GlyPro: 4.092 ± 0.478
2.642GlyGln: 2.642 ± 0.436
6.785GlyArg: 6.785 ± 0.645
4.817GlySer: 4.817 ± 0.636
6.474GlyThr: 6.474 ± 0.603
5.542GlyVal: 5.542 ± 0.463
1.916GlyTrp: 1.916 ± 0.245
2.072GlyTyr: 2.072 ± 0.362
0.0GlyXaa: 0.0 ± 0.0
His
2.331HisAla: 2.331 ± 0.349
0.518HisCys: 0.518 ± 0.173
1.554HisAsp: 1.554 ± 0.297
1.191HisGlu: 1.191 ± 0.277
0.466HisPhe: 0.466 ± 0.165
2.175HisGly: 2.175 ± 0.362
0.673HisHis: 0.673 ± 0.195
0.777HisIle: 0.777 ± 0.191
0.881HisLys: 0.881 ± 0.219
1.968HisLeu: 1.968 ± 0.25
0.466HisMet: 0.466 ± 0.156
0.673HisAsn: 0.673 ± 0.168
1.243HisPro: 1.243 ± 0.32
0.622HisGln: 0.622 ± 0.232
1.813HisArg: 1.813 ± 0.331
0.932HisSer: 0.932 ± 0.181
1.347HisThr: 1.347 ± 0.261
1.865HisVal: 1.865 ± 0.34
0.363HisTrp: 0.363 ± 0.172
0.414HisTyr: 0.414 ± 0.171
0.0HisXaa: 0.0 ± 0.0
Ile
5.283IleAla: 5.283 ± 0.43
0.207IleCys: 0.207 ± 0.12
4.04IleAsp: 4.04 ± 0.483
2.538IleGlu: 2.538 ± 0.419
0.725IlePhe: 0.725 ± 0.177
3.574IleGly: 3.574 ± 0.451
0.673IleHis: 0.673 ± 0.211
1.398IleIle: 1.398 ± 0.268
1.502IleLys: 1.502 ± 0.338
2.486IleLeu: 2.486 ± 0.339
0.57IleMet: 0.57 ± 0.166
1.502IleAsn: 1.502 ± 0.253
1.657IlePro: 1.657 ± 0.364
1.191IleGln: 1.191 ± 0.192
2.952IleArg: 2.952 ± 0.361
2.175IleSer: 2.175 ± 0.319
2.642IleThr: 2.642 ± 0.335
3.367IleVal: 3.367 ± 0.41
0.622IleTrp: 0.622 ± 0.209
0.932IleTyr: 0.932 ± 0.233
0.0IleXaa: 0.0 ± 0.0
Lys
4.144LysAla: 4.144 ± 0.629
0.363LysCys: 0.363 ± 0.135
0.984LysAsp: 0.984 ± 0.231
0.984LysGlu: 0.984 ± 0.201
1.191LysPhe: 1.191 ± 0.258
1.761LysGly: 1.761 ± 0.412
0.984LysHis: 0.984 ± 0.269
1.502LysIle: 1.502 ± 0.316
1.139LysLys: 1.139 ± 0.224
3.885LysLeu: 3.885 ± 0.403
0.984LysMet: 0.984 ± 0.215
1.347LysAsn: 1.347 ± 0.351
2.59LysPro: 2.59 ± 0.482
1.347LysGln: 1.347 ± 0.238
2.952LysArg: 2.952 ± 0.399
2.072LysSer: 2.072 ± 0.315
2.486LysThr: 2.486 ± 0.317
2.59LysVal: 2.59 ± 0.346
0.777LysTrp: 0.777 ± 0.186
0.725LysTyr: 0.725 ± 0.182
0.0LysXaa: 0.0 ± 0.0
Leu
10.618LeuAla: 10.618 ± 0.583
0.932LeuCys: 0.932 ± 0.223
6.164LeuAsp: 6.164 ± 0.469
3.418LeuGlu: 3.418 ± 0.31
2.331LeuPhe: 2.331 ± 0.356
5.905LeuGly: 5.905 ± 0.612
1.554LeuHis: 1.554 ± 0.272
2.901LeuIle: 2.901 ± 0.468
2.745LeuLys: 2.745 ± 0.318
5.387LeuLeu: 5.387 ± 0.62
1.295LeuMet: 1.295 ± 0.235
2.434LeuAsn: 2.434 ± 0.369
4.454LeuPro: 4.454 ± 0.464
3.367LeuGln: 3.367 ± 0.45
6.94LeuArg: 6.94 ± 0.917
4.558LeuSer: 4.558 ± 0.58
5.853LeuThr: 5.853 ± 0.508
5.076LeuVal: 5.076 ± 0.422
1.554LeuTrp: 1.554 ± 0.309
1.709LeuTyr: 1.709 ± 0.316
0.0LeuXaa: 0.0 ± 0.0
Met
2.02MetAla: 2.02 ± 0.272
0.207MetCys: 0.207 ± 0.095
0.777MetAsp: 0.777 ± 0.264
0.673MetGlu: 0.673 ± 0.177
0.881MetPhe: 0.881 ± 0.228
1.761MetGly: 1.761 ± 0.29
0.363MetHis: 0.363 ± 0.144
0.829MetIle: 0.829 ± 0.202
0.725MetLys: 0.725 ± 0.222
2.434MetLeu: 2.434 ± 0.399
0.311MetMet: 0.311 ± 0.125
0.932MetAsn: 0.932 ± 0.209
1.036MetPro: 1.036 ± 0.176
0.777MetGln: 0.777 ± 0.23
1.139MetArg: 1.139 ± 0.227
1.865MetSer: 1.865 ± 0.291
2.331MetThr: 2.331 ± 0.335
1.554MetVal: 1.554 ± 0.247
0.518MetTrp: 0.518 ± 0.172
0.518MetTyr: 0.518 ± 0.183
0.0MetXaa: 0.0 ± 0.0
Asn
3.781AsnAla: 3.781 ± 0.527
0.207AsnCys: 0.207 ± 0.095
1.916AsnAsp: 1.916 ± 0.34
1.295AsnGlu: 1.295 ± 0.258
0.414AsnPhe: 0.414 ± 0.206
3.833AsnGly: 3.833 ± 0.357
0.57AsnHis: 0.57 ± 0.187
1.036AsnIle: 1.036 ± 0.335
0.932AsnLys: 0.932 ± 0.189
1.916AsnLeu: 1.916 ± 0.391
0.673AsnMet: 0.673 ± 0.158
0.777AsnAsn: 0.777 ± 0.222
2.745AsnPro: 2.745 ± 0.33
0.881AsnGln: 0.881 ± 0.197
1.657AsnArg: 1.657 ± 0.307
1.088AsnSer: 1.088 ± 0.259
2.124AsnThr: 2.124 ± 0.373
2.02AsnVal: 2.02 ± 0.258
0.673AsnTrp: 0.673 ± 0.165
0.518AsnTyr: 0.518 ± 0.139
0.0AsnXaa: 0.0 ± 0.0
Pro
9.271ProAla: 9.271 ± 0.814
0.311ProCys: 0.311 ± 0.15
3.626ProAsp: 3.626 ± 0.556
3.263ProGlu: 3.263 ± 0.343
1.502ProPhe: 1.502 ± 0.329
4.299ProGly: 4.299 ± 0.491
1.243ProHis: 1.243 ± 0.245
2.59ProIle: 2.59 ± 0.29
2.538ProLys: 2.538 ± 0.485
3.677ProLeu: 3.677 ± 0.534
0.932ProMet: 0.932 ± 0.231
1.398ProAsn: 1.398 ± 0.256
2.538ProPro: 2.538 ± 0.38
1.968ProGln: 1.968 ± 0.43
3.263ProArg: 3.263 ± 0.496
2.59ProSer: 2.59 ± 0.347
3.367ProThr: 3.367 ± 0.381
3.626ProVal: 3.626 ± 0.411
0.829ProTrp: 0.829 ± 0.239
1.036ProTyr: 1.036 ± 0.246
0.0ProXaa: 0.0 ± 0.0
Gln
4.972GlnAla: 4.972 ± 0.614
0.311GlnCys: 0.311 ± 0.123
1.191GlnAsp: 1.191 ± 0.277
1.45GlnGlu: 1.45 ± 0.219
0.829GlnPhe: 0.829 ± 0.188
1.554GlnGly: 1.554 ± 0.303
1.243GlnHis: 1.243 ± 0.256
1.709GlnIle: 1.709 ± 0.277
1.139GlnLys: 1.139 ± 0.24
3.418GlnLeu: 3.418 ± 0.523
0.984GlnMet: 0.984 ± 0.215
0.725GlnAsn: 0.725 ± 0.206
2.124GlnPro: 2.124 ± 0.339
1.347GlnGln: 1.347 ± 0.351
3.626GlnArg: 3.626 ± 0.409
1.709GlnSer: 1.709 ± 0.307
1.606GlnThr: 1.606 ± 0.258
2.952GlnVal: 2.952 ± 0.393
0.881GlnTrp: 0.881 ± 0.214
0.932GlnTyr: 0.932 ± 0.218
0.0GlnXaa: 0.0 ± 0.0
Arg
8.391ArgAla: 8.391 ± 0.849
1.036ArgCys: 1.036 ± 0.238
4.817ArgAsp: 4.817 ± 0.557
3.781ArgGlu: 3.781 ± 0.541
2.279ArgPhe: 2.279 ± 0.322
5.749ArgGly: 5.749 ± 0.551
1.554ArgHis: 1.554 ± 0.305
4.195ArgIle: 4.195 ± 0.431
2.901ArgLys: 2.901 ± 0.38
5.853ArgLeu: 5.853 ± 0.519
2.124ArgMet: 2.124 ± 0.327
2.124ArgAsn: 2.124 ± 0.319
3.988ArgPro: 3.988 ± 0.573
3.159ArgGln: 3.159 ± 0.352
7.458ArgArg: 7.458 ± 0.904
3.522ArgSer: 3.522 ± 0.43
5.179ArgThr: 5.179 ± 0.53
5.697ArgVal: 5.697 ± 0.641
1.502ArgTrp: 1.502 ± 0.301
1.709ArgTyr: 1.709 ± 0.299
0.0ArgXaa: 0.0 ± 0.0
Ser
6.008SerAla: 6.008 ± 0.611
0.466SerCys: 0.466 ± 0.164
3.263SerAsp: 3.263 ± 0.373
3.211SerGlu: 3.211 ± 0.396
1.139SerPhe: 1.139 ± 0.217
4.351SerGly: 4.351 ± 0.645
0.777SerHis: 0.777 ± 0.22
2.02SerIle: 2.02 ± 0.389
1.502SerLys: 1.502 ± 0.215
3.159SerLeu: 3.159 ± 0.558
1.347SerMet: 1.347 ± 0.244
1.709SerAsn: 1.709 ± 0.353
2.175SerPro: 2.175 ± 0.334
1.657SerGln: 1.657 ± 0.326
3.056SerArg: 3.056 ± 0.366
2.693SerSer: 2.693 ± 0.513
3.988SerThr: 3.988 ± 0.531
4.092SerVal: 4.092 ± 0.514
1.709SerTrp: 1.709 ± 0.313
1.347SerTyr: 1.347 ± 0.246
0.0SerXaa: 0.0 ± 0.0
Thr
9.012ThrAla: 9.012 ± 0.752
0.725ThrCys: 0.725 ± 0.252
3.574ThrAsp: 3.574 ± 0.342
3.315ThrGlu: 3.315 ± 0.433
2.072ThrPhe: 2.072 ± 0.313
5.594ThrGly: 5.594 ± 0.619
1.606ThrHis: 1.606 ± 0.348
2.797ThrIle: 2.797 ± 0.42
2.538ThrLys: 2.538 ± 0.457
4.61ThrLeu: 4.61 ± 0.595
0.725ThrMet: 0.725 ± 0.197
1.761ThrAsn: 1.761 ± 0.258
3.988ThrPro: 3.988 ± 0.461
1.554ThrGln: 1.554 ± 0.252
4.454ThrArg: 4.454 ± 0.495
3.47ThrSer: 3.47 ± 0.47
4.247ThrThr: 4.247 ± 0.572
6.837ThrVal: 6.837 ± 0.651
1.347ThrTrp: 1.347 ± 0.283
1.554ThrTyr: 1.554 ± 0.295
0.0ThrXaa: 0.0 ± 0.0
Val
8.494ValAla: 8.494 ± 0.879
0.777ValCys: 0.777 ± 0.185
6.578ValAsp: 6.578 ± 0.766
4.662ValGlu: 4.662 ± 0.543
2.227ValPhe: 2.227 ± 0.321
5.801ValGly: 5.801 ± 0.603
2.383ValHis: 2.383 ± 0.352
2.797ValIle: 2.797 ± 0.367
3.056ValLys: 3.056 ± 0.368
5.853ValLeu: 5.853 ± 0.601
1.295ValMet: 1.295 ± 0.217
1.968ValAsn: 1.968 ± 0.283
4.092ValPro: 4.092 ± 0.477
2.383ValGln: 2.383 ± 0.315
5.438ValArg: 5.438 ± 0.562
3.729ValSer: 3.729 ± 0.502
5.024ValThr: 5.024 ± 0.619
6.474ValVal: 6.474 ± 0.576
1.243ValTrp: 1.243 ± 0.218
1.606ValTyr: 1.606 ± 0.284
0.0ValXaa: 0.0 ± 0.0
Trp
2.227TrpAla: 2.227 ± 0.392
0.311TrpCys: 0.311 ± 0.156
1.243TrpAsp: 1.243 ± 0.323
1.036TrpGlu: 1.036 ± 0.19
0.57TrpPhe: 0.57 ± 0.169
1.088TrpGly: 1.088 ± 0.277
0.363TrpHis: 0.363 ± 0.127
0.725TrpIle: 0.725 ± 0.183
0.466TrpLys: 0.466 ± 0.123
2.538TrpLeu: 2.538 ± 0.346
0.259TrpMet: 0.259 ± 0.117
0.673TrpAsn: 0.673 ± 0.181
1.036TrpPro: 1.036 ± 0.2
0.881TrpGln: 0.881 ± 0.256
1.865TrpArg: 1.865 ± 0.279
1.139TrpSer: 1.139 ± 0.246
1.761TrpThr: 1.761 ± 0.302
1.191TrpVal: 1.191 ± 0.229
0.311TrpTrp: 0.311 ± 0.136
0.414TrpTyr: 0.414 ± 0.138
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.331TyrAla: 2.331 ± 0.315
0.207TyrCys: 0.207 ± 0.089
1.398TyrAsp: 1.398 ± 0.303
1.295TyrGlu: 1.295 ± 0.259
0.57TyrPhe: 0.57 ± 0.126
2.434TyrGly: 2.434 ± 0.348
0.207TyrHis: 0.207 ± 0.087
0.725TyrIle: 0.725 ± 0.182
0.363TyrLys: 0.363 ± 0.125
1.968TyrLeu: 1.968 ± 0.294
0.259TyrMet: 0.259 ± 0.103
0.622TyrAsn: 0.622 ± 0.17
1.191TyrPro: 1.191 ± 0.226
1.036TyrGln: 1.036 ± 0.236
1.865TyrArg: 1.865 ± 0.308
1.347TyrSer: 1.347 ± 0.255
1.709TyrThr: 1.709 ± 0.36
1.657TyrVal: 1.657 ± 0.328
0.363TyrTrp: 0.363 ± 0.129
0.622TyrTyr: 0.622 ± 0.195
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 99 proteins (19308 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski