Hubei toti-like virus 13
Average proteome isoelectric point is 7.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KFE6|A0A1L3KFE6_9VIRU RNA-directed RNA polymerase OS=Hubei toti-like virus 13 OX=1923301 PE=4 SV=1
MM1 pKa = 7.59 SSSQQSDD8 pKa = 3.48 SKK10 pKa = 11.0 KK11 pKa = 10.85 DD12 pKa = 3.41 EE13 pKa = 4.49 GKK15 pKa = 8.49 TPSTPNQHH23 pKa = 6.38 SNNAPSSSTLLKK35 pKa = 9.95 QQQFFDD41 pKa = 4.26 PAHH44 pKa = 5.89 VLQSTTAQVLKK55 pKa = 9.53 TWFLHH60 pKa = 6.58 PYY62 pKa = 8.4 TGVGPHH68 pKa = 5.89 SVSFSQSLTNRR79 pKa = 11.84 DD80 pKa = 3.85 IINMYY85 pKa = 9.11 SQSGNYY91 pKa = 8.99 FYY93 pKa = 11.03 HH94 pKa = 6.43 SNCYY98 pKa = 8.84 SASYY102 pKa = 7.48 WTASSSYY109 pKa = 10.01 HH110 pKa = 4.95 QYY112 pKa = 11.2 AYY114 pKa = 9.96 KK115 pKa = 10.3 KK116 pKa = 9.98 KK117 pKa = 10.64 SSLTPSGGSSTPPAAVQSSVHH138 pKa = 7.15 SEE140 pKa = 3.74 DD141 pKa = 3.6 SYY143 pKa = 12.18 SFISDD148 pKa = 3.3 SGAGLLDD155 pKa = 3.48 IGPNVGIYY163 pKa = 8.86 YY164 pKa = 10.25 QNFDD168 pKa = 4.05 QIPSKK173 pKa = 10.77 VVEE176 pKa = 4.3 SLSPSVIKK184 pKa = 10.34 ILKK187 pKa = 10.07 EE188 pKa = 3.85 SDD190 pKa = 2.98 KK191 pKa = 11.45 HH192 pKa = 7.06 KK193 pKa = 10.13 LTLGRR198 pKa = 11.84 KK199 pKa = 8.72 LVLPSTWRR207 pKa = 11.84 KK208 pKa = 7.56 YY209 pKa = 9.25 QPFEE213 pKa = 3.59 ATRR216 pKa = 11.84 WGKK219 pKa = 9.78 NSKK222 pKa = 10.74 DD223 pKa = 3.99 KK224 pKa = 11.0 INQAGSSEE232 pKa = 4.3 TEE234 pKa = 3.26 IDD236 pKa = 4.5 LNTVTCWPRR245 pKa = 11.84 IDD247 pKa = 3.62 IKK249 pKa = 11.3 YY250 pKa = 9.11 PNKK253 pKa = 10.6 DD254 pKa = 3.27 SVKK257 pKa = 10.25 FADD260 pKa = 4.86 KK261 pKa = 10.59 KK262 pKa = 8.47 WTPLADD268 pKa = 3.11 KK269 pKa = 10.52 SAYY272 pKa = 7.74 YY273 pKa = 8.56 TQAPIPSDD281 pKa = 3.65 KK282 pKa = 10.68 FPEE285 pKa = 4.15 ALGIEE290 pKa = 4.98 GIDD293 pKa = 3.61 DD294 pKa = 3.53 ATFQGNLDD302 pKa = 4.17 CGDD305 pKa = 3.94 TVPVRR310 pKa = 11.84 SITRR314 pKa = 11.84 TFSMLPSDD322 pKa = 3.51 IDD324 pKa = 3.82 ANRR327 pKa = 11.84 SIPTTNVYY335 pKa = 10.63 SQFLVKK341 pKa = 9.63 LTKK344 pKa = 10.37 DD345 pKa = 3.49 LPDD348 pKa = 3.81 DD349 pKa = 4.2 AFRR352 pKa = 11.84 SMISRR357 pKa = 11.84 PAITDD362 pKa = 3.13 ADD364 pKa = 3.81 SRR366 pKa = 11.84 IWLAYY371 pKa = 10.08 NLSQLKK377 pKa = 9.51 YY378 pKa = 9.2 GSYY381 pKa = 10.0 MDD383 pKa = 4.77 VIPPTVAMLLLHH395 pKa = 6.67 SLTADD400 pKa = 3.67 RR401 pKa = 11.84 HH402 pKa = 4.71 WVDD405 pKa = 4.24 PIIFRR410 pKa = 11.84 TIRR413 pKa = 11.84 NKK415 pKa = 10.45 SIRR418 pKa = 11.84 AYY420 pKa = 10.46 DD421 pKa = 3.55 KK422 pKa = 11.0 DD423 pKa = 3.25 IAISARR429 pKa = 11.84 PATEE433 pKa = 3.93 YY434 pKa = 10.6 PNLIVVSSHH443 pKa = 5.11 LTHH446 pKa = 6.48 FVKK449 pKa = 10.79 YY450 pKa = 7.94 MSRR453 pKa = 11.84 TVPVHH458 pKa = 7.68 DD459 pKa = 4.43 SFDD462 pKa = 4.36 PNMIDD467 pKa = 4.7 DD468 pKa = 4.45 DD469 pKa = 4.68 WIAVPVTTEE478 pKa = 4.06 LLAVPTKK485 pKa = 10.73 LGAFVMCHH493 pKa = 6.46 LSSEE497 pKa = 4.55 FWNGTITWSKK507 pKa = 8.85 KK508 pKa = 8.59 HH509 pKa = 6.39 AYY511 pKa = 9.81 SIPDD515 pKa = 3.17 SMIPDD520 pKa = 4.33 GQAEE524 pKa = 4.89 GYY526 pKa = 8.46 KK527 pKa = 10.3 QKK529 pKa = 10.72 FRR531 pKa = 11.84 MGKK534 pKa = 8.9 EE535 pKa = 3.66 YY536 pKa = 10.62 FMPSSNSVYY545 pKa = 9.77 IDD547 pKa = 3.41 GVKK550 pKa = 10.31 KK551 pKa = 9.39 VWLVIMPSTSNATATIKK568 pKa = 10.61 LYY570 pKa = 9.26 NTTVPRR576 pKa = 11.84 SPDD579 pKa = 3.22 KK580 pKa = 11.0 DD581 pKa = 3.42 GKK583 pKa = 10.85 LSPKK587 pKa = 10.48 DD588 pKa = 3.72 LTPFWATYY596 pKa = 7.84 WQGTDD601 pKa = 3.36 RR602 pKa = 11.84 DD603 pKa = 4.62 NIPLLITDD611 pKa = 5.0 FFWALCAMTNSTVTPDD627 pKa = 3.13 STRR630 pKa = 11.84 RR631 pKa = 11.84 AMGLATEE638 pKa = 5.04 LCNVSYY644 pKa = 10.29 PGNRR648 pKa = 11.84 FQNSTEE654 pKa = 4.05 VQPALIGGAWTYY666 pKa = 11.24 GGQKK670 pKa = 9.54 VFKK673 pKa = 9.98 NKK675 pKa = 9.7 YY676 pKa = 9.74 DD677 pKa = 3.49 SSDD680 pKa = 3.0 WVDD683 pKa = 2.88 AHH685 pKa = 7.48 IPVKK689 pKa = 10.26 NASYY693 pKa = 10.15 PRR695 pKa = 11.84 RR696 pKa = 11.84 TRR698 pKa = 11.84 MAGFSFSATSPFLQHH713 pKa = 6.67 PSSLSTLTQNLYY725 pKa = 10.71 LYY727 pKa = 9.91 NLGMTTGEE735 pKa = 4.13 DD736 pKa = 3.35 QVVKK740 pKa = 9.94 QIWVIDD746 pKa = 4.24 KK747 pKa = 10.32 IRR749 pKa = 11.84 QRR751 pKa = 11.84 WATTQPTYY759 pKa = 11.05 NVPQYY764 pKa = 10.28 IANQATSFCRR774 pKa = 11.84 LSVAAGFLEE783 pKa = 4.43 TGGGRR788 pKa = 11.84 EE789 pKa = 4.03 YY790 pKa = 10.8 RR791 pKa = 11.84 FSVSPAVQQFLTHH804 pKa = 6.48 NGAAMFGNTTNFLIANDD821 pKa = 3.64 IQPWFWMGYY830 pKa = 9.44 GYY832 pKa = 10.88 GDD834 pKa = 3.66 HH835 pKa = 7.14 PHH837 pKa = 6.77 FLDD840 pKa = 5.26 AYY842 pKa = 8.75 ATLFKK847 pKa = 10.87 QLTQSMMLPVNIYY860 pKa = 10.44 NLAISCQNLDD870 pKa = 3.01 WNNIVEE876 pKa = 4.7 YY877 pKa = 10.99 YY878 pKa = 9.38 NTDD881 pKa = 3.17 PFDD884 pKa = 3.45 QEE886 pKa = 3.79 RR887 pKa = 11.84 WMQSAPIPVSNYY899 pKa = 9.12 LQWNQKK905 pKa = 8.56 MDD907 pKa = 4.08 LPQTPNTGEE916 pKa = 4.05 VQPVQLFGKK925 pKa = 9.39 QLYY928 pKa = 9.44 GLKK931 pKa = 10.13 LDD933 pKa = 4.36 RR934 pKa = 11.84 TNSDD938 pKa = 2.7 LKK940 pKa = 11.24 ARR942 pKa = 11.84 IFLLVNDD949 pKa = 5.4 RR950 pKa = 11.84 KK951 pKa = 8.88 TEE953 pKa = 3.88 WPKK956 pKa = 10.66 ATVFDD961 pKa = 5.24 ANDD964 pKa = 3.33 EE965 pKa = 4.4 TPYY968 pKa = 10.88 NEE970 pKa = 4.13 WMWLDD975 pKa = 4.2 DD976 pKa = 4.3 YY977 pKa = 11.77 YY978 pKa = 11.76 FMSTALVDD986 pKa = 3.91 AGQMVTTKK994 pKa = 9.86 WDD996 pKa = 3.25 GNAYY1000 pKa = 10.36 LSSNTYY1006 pKa = 10.44 ARR1008 pKa = 11.84 STSPGVLGYY1017 pKa = 9.82 EE1018 pKa = 4.06 AEE1020 pKa = 4.22 TLIVVTSGLGLGRR1033 pKa = 11.84 NAVQSLPHH1041 pKa = 5.3 VTPVSLPDD1049 pKa = 3.97 PPDD1052 pKa = 3.47 AKK1054 pKa = 10.2 TFLAAKK1060 pKa = 9.7 VADD1063 pKa = 4.56 SNPPPPPEE1071 pKa = 4.29 MKK1073 pKa = 10.19 SDD1075 pKa = 5.17 PIPTKK1080 pKa = 9.37 EE1081 pKa = 4.0 QVSAVTDD1088 pKa = 3.5 AVTPVTDD1095 pKa = 4.94 TITQAA1100 pKa = 3.32
Molecular weight: 123.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.09
IPC2_protein 6.084
IPC_protein 6.186
Toseland 6.576
ProMoST 6.59
Dawson 6.532
Bjellqvist 6.504
Wikipedia 6.547
Rodwell 6.517
Grimsley 6.751
Solomon 6.532
Lehninger 6.517
Nozaki 6.795
DTASelect 6.985
Thurlkill 7.015
EMBOSS 6.985
Sillero 6.912
Patrickios 3.973
IPC_peptide 6.547
IPC2_peptide 6.722
IPC2.peptide.svr19 6.791
Protein with the highest isoelectric point:
>tr|A0A1L3KFE6|A0A1L3KFE6_9VIRU RNA-directed RNA polymerase OS=Hubei toti-like virus 13 OX=1923301 PE=4 SV=1
MM1 pKa = 7.43 CHH3 pKa = 6.77 LHH5 pKa = 5.87 TLGGPSPYY13 pKa = 9.44 TKK15 pKa = 10.41 KK16 pKa = 10.5 QIHH19 pKa = 7.42 DD20 pKa = 5.67 DD21 pKa = 3.12 IDD23 pKa = 3.46 SWVKK27 pKa = 11.17 GEE29 pKa = 4.29 IDD31 pKa = 3.34 TFPRR35 pKa = 11.84 NWTLEE40 pKa = 4.07 QVDD43 pKa = 4.93 KK44 pKa = 11.36 IFMSTKK50 pKa = 10.22 RR51 pKa = 11.84 LHH53 pKa = 6.08 QSNALTFSDD62 pKa = 3.96 YY63 pKa = 11.52 SKK65 pKa = 11.41 DD66 pKa = 3.51 VLRR69 pKa = 11.84 WATSGGAPASHH80 pKa = 7.13 IGGQKK85 pKa = 9.65 VRR87 pKa = 11.84 SKK89 pKa = 9.7 WAWGFSRR96 pKa = 11.84 LGDD99 pKa = 3.4 INSPKK104 pKa = 10.24 VGEE107 pKa = 4.46 AIARR111 pKa = 11.84 EE112 pKa = 4.16 SLTTEE117 pKa = 4.13 GKK119 pKa = 8.63 YY120 pKa = 8.49 TVALKK125 pKa = 10.66 RR126 pKa = 11.84 EE127 pKa = 3.97 PAKK130 pKa = 10.09 TRR132 pKa = 11.84 EE133 pKa = 4.33 IISGPMASHH142 pKa = 6.95 IRR144 pKa = 11.84 QSYY147 pKa = 9.55 LIYY150 pKa = 10.02 RR151 pKa = 11.84 TTNWDD156 pKa = 3.58 LPSPAFNKK164 pKa = 8.81 TAYY167 pKa = 7.96 QHH169 pKa = 6.33 YY170 pKa = 9.02 MEE172 pKa = 5.02 KK173 pKa = 10.42 QFTSYY178 pKa = 11.03 CSIDD182 pKa = 3.66 GDD184 pKa = 3.69 RR185 pKa = 11.84 FDD187 pKa = 5.68 HH188 pKa = 6.81 NVPKK192 pKa = 10.29 WFVLEE197 pKa = 4.38 FVRR200 pKa = 11.84 RR201 pKa = 11.84 LAFDD205 pKa = 3.89 EE206 pKa = 4.09 EE207 pKa = 4.48 TQRR210 pKa = 11.84 VVDD213 pKa = 5.19 LEE215 pKa = 4.48 IIHH218 pKa = 6.69 LNSAYY223 pKa = 10.85 LEE225 pKa = 4.26 FEE227 pKa = 3.81 GRR229 pKa = 11.84 RR230 pKa = 11.84 WEE232 pKa = 4.17 WQHH235 pKa = 6.09 GLLSGWRR242 pKa = 11.84 ITSLLGTVISHH253 pKa = 7.49 IAALWIKK260 pKa = 10.47 QSTGLFFQHH269 pKa = 6.68 LVLGDD274 pKa = 4.51 DD275 pKa = 4.14 IILYY279 pKa = 10.55 SDD281 pKa = 3.4 VDD283 pKa = 3.89 EE284 pKa = 5.83 LNVSQCVDD292 pKa = 3.0 AYY294 pKa = 10.13 KK295 pKa = 10.93 SFGIPANKK303 pKa = 9.69 SKK305 pKa = 10.79 SISGRR310 pKa = 11.84 TGEE313 pKa = 4.42 FLRR316 pKa = 11.84 KK317 pKa = 9.11 IATPDD322 pKa = 4.04 GILGYY327 pKa = 9.14 PALALKK333 pKa = 10.14 SVYY336 pKa = 9.8 YY337 pKa = 10.54 ASPWLNEE344 pKa = 4.0 YY345 pKa = 10.29 KK346 pKa = 9.63 RR347 pKa = 11.84 TVQSEE352 pKa = 4.34 ISHH355 pKa = 5.64 SWWTFCSRR363 pKa = 11.84 MLPHH367 pKa = 6.66 RR368 pKa = 11.84 TNNNIIMSIIEE379 pKa = 5.01 RR380 pKa = 11.84 MNQDD384 pKa = 1.73 IWFTDD389 pKa = 3.33 DD390 pKa = 5.27 QFYY393 pKa = 10.8 KK394 pKa = 10.19 WVQTPMCMGGGGILEE409 pKa = 4.53 TSSEE413 pKa = 4.49 QYY415 pKa = 8.67 WLSYY419 pKa = 10.52 LVNTDD424 pKa = 3.04 SKK426 pKa = 11.28 LDD428 pKa = 3.7 RR429 pKa = 11.84 NAYY432 pKa = 9.58 FYY434 pKa = 11.38 SLFGIGDD441 pKa = 3.37 KK442 pKa = 10.64 SVYY445 pKa = 10.05 RR446 pKa = 11.84 KK447 pKa = 9.01 PQFKK451 pKa = 10.44 KK452 pKa = 9.71 IVPSRR457 pKa = 11.84 FSISHH462 pKa = 6.06 MNHH465 pKa = 6.98 DD466 pKa = 4.97 FPTKK470 pKa = 10.17 PSIPDD475 pKa = 3.94 NINKK479 pKa = 9.07 TIIIMRR485 pKa = 11.84 WYY487 pKa = 10.05 FNRR490 pKa = 11.84 YY491 pKa = 8.04 PNSFLQQGGISFSRR505 pKa = 11.84 SVRR508 pKa = 11.84 GLSNTKK514 pKa = 10.23 LLEE517 pKa = 4.2 ILLGQSDD524 pKa = 4.45 RR525 pKa = 11.84 LTSVVSLLHH534 pKa = 7.31 VGEE537 pKa = 4.12 QLSDD541 pKa = 4.08 KK542 pKa = 10.64 ISSTLGMLSTLRR554 pKa = 11.84 PNGPVSQLVADD565 pKa = 4.07 MYY567 pKa = 11.56 VFLEE571 pKa = 3.95 RR572 pKa = 11.84 FLEE575 pKa = 4.21 NKK577 pKa = 9.53 EE578 pKa = 4.06 LSLVTWW584 pKa = 4.24
Molecular weight: 67.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.23
IPC2_protein 8.273
IPC_protein 8.17
Toseland 8.624
ProMoST 8.639
Dawson 9.019
Bjellqvist 8.946
Wikipedia 9.18
Rodwell 9.136
Grimsley 8.99
Solomon 9.107
Lehninger 9.063
Nozaki 8.975
DTASelect 8.843
Thurlkill 8.916
EMBOSS 9.151
Sillero 9.107
Patrickios 4.457
IPC_peptide 9.092
IPC2_peptide 7.658
IPC2.peptide.svr19 7.638
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1684
584
1100
842.0
95.15
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.879 ± 0.99
0.772 ± 0.047
6.235 ± 0.519
3.325 ± 0.726
4.276 ± 0.29
5.404 ± 0.521
2.257 ± 0.366
5.76 ± 0.801
5.582 ± 0.038
8.076 ± 0.751
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.375 ± 0.084
4.751 ± 0.359
6.057 ± 1.09
4.454 ± 0.288
4.157 ± 0.836
10.036 ± 0.133
7.542 ± 0.866
5.76 ± 0.349
2.791 ± 0.259
4.513 ± 0.226
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here