Hubei toti-like virus 13

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KFE6|A0A1L3KFE6_9VIRU RNA-directed RNA polymerase OS=Hubei toti-like virus 13 OX=1923301 PE=4 SV=1
MM1 pKa = 7.59SSSQQSDD8 pKa = 3.48SKK10 pKa = 11.0KK11 pKa = 10.85DD12 pKa = 3.41EE13 pKa = 4.49GKK15 pKa = 8.49TPSTPNQHH23 pKa = 6.38SNNAPSSSTLLKK35 pKa = 9.95QQQFFDD41 pKa = 4.26PAHH44 pKa = 5.89VLQSTTAQVLKK55 pKa = 9.53TWFLHH60 pKa = 6.58PYY62 pKa = 8.4TGVGPHH68 pKa = 5.89SVSFSQSLTNRR79 pKa = 11.84DD80 pKa = 3.85IINMYY85 pKa = 9.11SQSGNYY91 pKa = 8.99FYY93 pKa = 11.03HH94 pKa = 6.43SNCYY98 pKa = 8.84SASYY102 pKa = 7.48WTASSSYY109 pKa = 10.01HH110 pKa = 4.95QYY112 pKa = 11.2AYY114 pKa = 9.96KK115 pKa = 10.3KK116 pKa = 9.98KK117 pKa = 10.64SSLTPSGGSSTPPAAVQSSVHH138 pKa = 7.15SEE140 pKa = 3.74DD141 pKa = 3.6SYY143 pKa = 12.18SFISDD148 pKa = 3.3SGAGLLDD155 pKa = 3.48IGPNVGIYY163 pKa = 8.86YY164 pKa = 10.25QNFDD168 pKa = 4.05QIPSKK173 pKa = 10.77VVEE176 pKa = 4.3SLSPSVIKK184 pKa = 10.34ILKK187 pKa = 10.07EE188 pKa = 3.85SDD190 pKa = 2.98KK191 pKa = 11.45HH192 pKa = 7.06KK193 pKa = 10.13LTLGRR198 pKa = 11.84KK199 pKa = 8.72LVLPSTWRR207 pKa = 11.84KK208 pKa = 7.56YY209 pKa = 9.25QPFEE213 pKa = 3.59ATRR216 pKa = 11.84WGKK219 pKa = 9.78NSKK222 pKa = 10.74DD223 pKa = 3.99KK224 pKa = 11.0INQAGSSEE232 pKa = 4.3TEE234 pKa = 3.26IDD236 pKa = 4.5LNTVTCWPRR245 pKa = 11.84IDD247 pKa = 3.62IKK249 pKa = 11.3YY250 pKa = 9.11PNKK253 pKa = 10.6DD254 pKa = 3.27SVKK257 pKa = 10.25FADD260 pKa = 4.86KK261 pKa = 10.59KK262 pKa = 8.47WTPLADD268 pKa = 3.11KK269 pKa = 10.52SAYY272 pKa = 7.74YY273 pKa = 8.56TQAPIPSDD281 pKa = 3.65KK282 pKa = 10.68FPEE285 pKa = 4.15ALGIEE290 pKa = 4.98GIDD293 pKa = 3.61DD294 pKa = 3.53ATFQGNLDD302 pKa = 4.17CGDD305 pKa = 3.94TVPVRR310 pKa = 11.84SITRR314 pKa = 11.84TFSMLPSDD322 pKa = 3.51IDD324 pKa = 3.82ANRR327 pKa = 11.84SIPTTNVYY335 pKa = 10.63SQFLVKK341 pKa = 9.63LTKK344 pKa = 10.37DD345 pKa = 3.49LPDD348 pKa = 3.81DD349 pKa = 4.2AFRR352 pKa = 11.84SMISRR357 pKa = 11.84PAITDD362 pKa = 3.13ADD364 pKa = 3.81SRR366 pKa = 11.84IWLAYY371 pKa = 10.08NLSQLKK377 pKa = 9.51YY378 pKa = 9.2GSYY381 pKa = 10.0MDD383 pKa = 4.77VIPPTVAMLLLHH395 pKa = 6.67SLTADD400 pKa = 3.67RR401 pKa = 11.84HH402 pKa = 4.71WVDD405 pKa = 4.24PIIFRR410 pKa = 11.84TIRR413 pKa = 11.84NKK415 pKa = 10.45SIRR418 pKa = 11.84AYY420 pKa = 10.46DD421 pKa = 3.55KK422 pKa = 11.0DD423 pKa = 3.25IAISARR429 pKa = 11.84PATEE433 pKa = 3.93YY434 pKa = 10.6PNLIVVSSHH443 pKa = 5.11LTHH446 pKa = 6.48FVKK449 pKa = 10.79YY450 pKa = 7.94MSRR453 pKa = 11.84TVPVHH458 pKa = 7.68DD459 pKa = 4.43SFDD462 pKa = 4.36PNMIDD467 pKa = 4.7DD468 pKa = 4.45DD469 pKa = 4.68WIAVPVTTEE478 pKa = 4.06LLAVPTKK485 pKa = 10.73LGAFVMCHH493 pKa = 6.46LSSEE497 pKa = 4.55FWNGTITWSKK507 pKa = 8.85KK508 pKa = 8.59HH509 pKa = 6.39AYY511 pKa = 9.81SIPDD515 pKa = 3.17SMIPDD520 pKa = 4.33GQAEE524 pKa = 4.89GYY526 pKa = 8.46KK527 pKa = 10.3QKK529 pKa = 10.72FRR531 pKa = 11.84MGKK534 pKa = 8.9EE535 pKa = 3.66YY536 pKa = 10.62FMPSSNSVYY545 pKa = 9.77IDD547 pKa = 3.41GVKK550 pKa = 10.31KK551 pKa = 9.39VWLVIMPSTSNATATIKK568 pKa = 10.61LYY570 pKa = 9.26NTTVPRR576 pKa = 11.84SPDD579 pKa = 3.22KK580 pKa = 11.0DD581 pKa = 3.42GKK583 pKa = 10.85LSPKK587 pKa = 10.48DD588 pKa = 3.72LTPFWATYY596 pKa = 7.84WQGTDD601 pKa = 3.36RR602 pKa = 11.84DD603 pKa = 4.62NIPLLITDD611 pKa = 5.0FFWALCAMTNSTVTPDD627 pKa = 3.13STRR630 pKa = 11.84RR631 pKa = 11.84AMGLATEE638 pKa = 5.04LCNVSYY644 pKa = 10.29PGNRR648 pKa = 11.84FQNSTEE654 pKa = 4.05VQPALIGGAWTYY666 pKa = 11.24GGQKK670 pKa = 9.54VFKK673 pKa = 9.98NKK675 pKa = 9.7YY676 pKa = 9.74DD677 pKa = 3.49SSDD680 pKa = 3.0WVDD683 pKa = 2.88AHH685 pKa = 7.48IPVKK689 pKa = 10.26NASYY693 pKa = 10.15PRR695 pKa = 11.84RR696 pKa = 11.84TRR698 pKa = 11.84MAGFSFSATSPFLQHH713 pKa = 6.67PSSLSTLTQNLYY725 pKa = 10.71LYY727 pKa = 9.91NLGMTTGEE735 pKa = 4.13DD736 pKa = 3.35QVVKK740 pKa = 9.94QIWVIDD746 pKa = 4.24KK747 pKa = 10.32IRR749 pKa = 11.84QRR751 pKa = 11.84WATTQPTYY759 pKa = 11.05NVPQYY764 pKa = 10.28IANQATSFCRR774 pKa = 11.84LSVAAGFLEE783 pKa = 4.43TGGGRR788 pKa = 11.84EE789 pKa = 4.03YY790 pKa = 10.8RR791 pKa = 11.84FSVSPAVQQFLTHH804 pKa = 6.48NGAAMFGNTTNFLIANDD821 pKa = 3.64IQPWFWMGYY830 pKa = 9.44GYY832 pKa = 10.88GDD834 pKa = 3.66HH835 pKa = 7.14PHH837 pKa = 6.77FLDD840 pKa = 5.26AYY842 pKa = 8.75ATLFKK847 pKa = 10.87QLTQSMMLPVNIYY860 pKa = 10.44NLAISCQNLDD870 pKa = 3.01WNNIVEE876 pKa = 4.7YY877 pKa = 10.99YY878 pKa = 9.38NTDD881 pKa = 3.17PFDD884 pKa = 3.45QEE886 pKa = 3.79RR887 pKa = 11.84WMQSAPIPVSNYY899 pKa = 9.12LQWNQKK905 pKa = 8.56MDD907 pKa = 4.08LPQTPNTGEE916 pKa = 4.05VQPVQLFGKK925 pKa = 9.39QLYY928 pKa = 9.44GLKK931 pKa = 10.13LDD933 pKa = 4.36RR934 pKa = 11.84TNSDD938 pKa = 2.7LKK940 pKa = 11.24ARR942 pKa = 11.84IFLLVNDD949 pKa = 5.4RR950 pKa = 11.84KK951 pKa = 8.88TEE953 pKa = 3.88WPKK956 pKa = 10.66ATVFDD961 pKa = 5.24ANDD964 pKa = 3.33EE965 pKa = 4.4TPYY968 pKa = 10.88NEE970 pKa = 4.13WMWLDD975 pKa = 4.2DD976 pKa = 4.3YY977 pKa = 11.77YY978 pKa = 11.76FMSTALVDD986 pKa = 3.91AGQMVTTKK994 pKa = 9.86WDD996 pKa = 3.25GNAYY1000 pKa = 10.36LSSNTYY1006 pKa = 10.44ARR1008 pKa = 11.84STSPGVLGYY1017 pKa = 9.82EE1018 pKa = 4.06AEE1020 pKa = 4.22TLIVVTSGLGLGRR1033 pKa = 11.84NAVQSLPHH1041 pKa = 5.3VTPVSLPDD1049 pKa = 3.97PPDD1052 pKa = 3.47AKK1054 pKa = 10.2TFLAAKK1060 pKa = 9.7VADD1063 pKa = 4.56SNPPPPPEE1071 pKa = 4.29MKK1073 pKa = 10.19SDD1075 pKa = 5.17PIPTKK1080 pKa = 9.37EE1081 pKa = 4.0QVSAVTDD1088 pKa = 3.5AVTPVTDD1095 pKa = 4.94TITQAA1100 pKa = 3.32

Molecular weight:
123.16 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KFE6|A0A1L3KFE6_9VIRU RNA-directed RNA polymerase OS=Hubei toti-like virus 13 OX=1923301 PE=4 SV=1
MM1 pKa = 7.43CHH3 pKa = 6.77LHH5 pKa = 5.87TLGGPSPYY13 pKa = 9.44TKK15 pKa = 10.41KK16 pKa = 10.5QIHH19 pKa = 7.42DD20 pKa = 5.67DD21 pKa = 3.12IDD23 pKa = 3.46SWVKK27 pKa = 11.17GEE29 pKa = 4.29IDD31 pKa = 3.34TFPRR35 pKa = 11.84NWTLEE40 pKa = 4.07QVDD43 pKa = 4.93KK44 pKa = 11.36IFMSTKK50 pKa = 10.22RR51 pKa = 11.84LHH53 pKa = 6.08QSNALTFSDD62 pKa = 3.96YY63 pKa = 11.52SKK65 pKa = 11.41DD66 pKa = 3.51VLRR69 pKa = 11.84WATSGGAPASHH80 pKa = 7.13IGGQKK85 pKa = 9.65VRR87 pKa = 11.84SKK89 pKa = 9.7WAWGFSRR96 pKa = 11.84LGDD99 pKa = 3.4INSPKK104 pKa = 10.24VGEE107 pKa = 4.46AIARR111 pKa = 11.84EE112 pKa = 4.16SLTTEE117 pKa = 4.13GKK119 pKa = 8.63YY120 pKa = 8.49TVALKK125 pKa = 10.66RR126 pKa = 11.84EE127 pKa = 3.97PAKK130 pKa = 10.09TRR132 pKa = 11.84EE133 pKa = 4.33IISGPMASHH142 pKa = 6.95IRR144 pKa = 11.84QSYY147 pKa = 9.55LIYY150 pKa = 10.02RR151 pKa = 11.84TTNWDD156 pKa = 3.58LPSPAFNKK164 pKa = 8.81TAYY167 pKa = 7.96QHH169 pKa = 6.33YY170 pKa = 9.02MEE172 pKa = 5.02KK173 pKa = 10.42QFTSYY178 pKa = 11.03CSIDD182 pKa = 3.66GDD184 pKa = 3.69RR185 pKa = 11.84FDD187 pKa = 5.68HH188 pKa = 6.81NVPKK192 pKa = 10.29WFVLEE197 pKa = 4.38FVRR200 pKa = 11.84RR201 pKa = 11.84LAFDD205 pKa = 3.89EE206 pKa = 4.09EE207 pKa = 4.48TQRR210 pKa = 11.84VVDD213 pKa = 5.19LEE215 pKa = 4.48IIHH218 pKa = 6.69LNSAYY223 pKa = 10.85LEE225 pKa = 4.26FEE227 pKa = 3.81GRR229 pKa = 11.84RR230 pKa = 11.84WEE232 pKa = 4.17WQHH235 pKa = 6.09GLLSGWRR242 pKa = 11.84ITSLLGTVISHH253 pKa = 7.49IAALWIKK260 pKa = 10.47QSTGLFFQHH269 pKa = 6.68LVLGDD274 pKa = 4.51DD275 pKa = 4.14IILYY279 pKa = 10.55SDD281 pKa = 3.4VDD283 pKa = 3.89EE284 pKa = 5.83LNVSQCVDD292 pKa = 3.0AYY294 pKa = 10.13KK295 pKa = 10.93SFGIPANKK303 pKa = 9.69SKK305 pKa = 10.79SISGRR310 pKa = 11.84TGEE313 pKa = 4.42FLRR316 pKa = 11.84KK317 pKa = 9.11IATPDD322 pKa = 4.04GILGYY327 pKa = 9.14PALALKK333 pKa = 10.14SVYY336 pKa = 9.8YY337 pKa = 10.54ASPWLNEE344 pKa = 4.0YY345 pKa = 10.29KK346 pKa = 9.63RR347 pKa = 11.84TVQSEE352 pKa = 4.34ISHH355 pKa = 5.64SWWTFCSRR363 pKa = 11.84MLPHH367 pKa = 6.66RR368 pKa = 11.84TNNNIIMSIIEE379 pKa = 5.01RR380 pKa = 11.84MNQDD384 pKa = 1.73IWFTDD389 pKa = 3.33DD390 pKa = 5.27QFYY393 pKa = 10.8KK394 pKa = 10.19WVQTPMCMGGGGILEE409 pKa = 4.53TSSEE413 pKa = 4.49QYY415 pKa = 8.67WLSYY419 pKa = 10.52LVNTDD424 pKa = 3.04SKK426 pKa = 11.28LDD428 pKa = 3.7RR429 pKa = 11.84NAYY432 pKa = 9.58FYY434 pKa = 11.38SLFGIGDD441 pKa = 3.37KK442 pKa = 10.64SVYY445 pKa = 10.05RR446 pKa = 11.84KK447 pKa = 9.01PQFKK451 pKa = 10.44KK452 pKa = 9.71IVPSRR457 pKa = 11.84FSISHH462 pKa = 6.06MNHH465 pKa = 6.98DD466 pKa = 4.97FPTKK470 pKa = 10.17PSIPDD475 pKa = 3.94NINKK479 pKa = 9.07TIIIMRR485 pKa = 11.84WYY487 pKa = 10.05FNRR490 pKa = 11.84YY491 pKa = 8.04PNSFLQQGGISFSRR505 pKa = 11.84SVRR508 pKa = 11.84GLSNTKK514 pKa = 10.23LLEE517 pKa = 4.2ILLGQSDD524 pKa = 4.45RR525 pKa = 11.84LTSVVSLLHH534 pKa = 7.31VGEE537 pKa = 4.12QLSDD541 pKa = 4.08KK542 pKa = 10.64ISSTLGMLSTLRR554 pKa = 11.84PNGPVSQLVADD565 pKa = 4.07MYY567 pKa = 11.56VFLEE571 pKa = 3.95RR572 pKa = 11.84FLEE575 pKa = 4.21NKK577 pKa = 9.53EE578 pKa = 4.06LSLVTWW584 pKa = 4.24

Molecular weight:
67.13 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1684

584

1100

842.0

95.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.879 ± 0.99

0.772 ± 0.047

6.235 ± 0.519

3.325 ± 0.726

4.276 ± 0.29

5.404 ± 0.521

2.257 ± 0.366

5.76 ± 0.801

5.582 ± 0.038

8.076 ± 0.751

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.375 ± 0.084

4.751 ± 0.359

6.057 ± 1.09

4.454 ± 0.288

4.157 ± 0.836

10.036 ± 0.133

7.542 ± 0.866

5.76 ± 0.349

2.791 ± 0.259

4.513 ± 0.226

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski