Pandoraea sputorum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Pandoraea

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5005 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A239SG55|A0A239SG55_9BURK Acetylornithine deacetylase OS=Pandoraea sputorum OX=93222 GN=argE PE=4 SV=1
MM1 pKa = 7.6ANFYY5 pKa = 9.19EE6 pKa = 4.97IPFSPAPQAFRR17 pKa = 11.84VTLSGVEE24 pKa = 3.98YY25 pKa = 9.64TLTVQYY31 pKa = 10.5RR32 pKa = 11.84AADD35 pKa = 3.64DD36 pKa = 4.54AGWMLDD42 pKa = 3.17IADD45 pKa = 4.57ANGAPIIGGLPLVTGTDD62 pKa = 3.4LLAPYY67 pKa = 9.95AYY69 pKa = 10.25LGLGGRR75 pKa = 11.84LWVQGADD82 pKa = 3.77NPDD85 pKa = 3.3NTPTFDD91 pKa = 3.81DD92 pKa = 4.66LGVGSHH98 pKa = 5.71VLWVTDD104 pKa = 3.42

Molecular weight: 11.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A239SZR5|A0A239SZR5_9BURK Glycine dehydrogenase (decarboxylating) OS=Pandoraea sputorum OX=93222 GN=gcvP PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.43RR14 pKa = 11.84THH16 pKa = 5.88GFLVRR21 pKa = 11.84MKK23 pKa = 9.86TRR25 pKa = 11.84GGRR28 pKa = 11.84KK29 pKa = 8.93VIAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.8GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5005

0

5005

1675428

29

4562

334.8

36.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.598 ± 0.045

0.887 ± 0.012

5.451 ± 0.028

4.867 ± 0.037

3.607 ± 0.025

8.372 ± 0.052

2.231 ± 0.018

4.595 ± 0.026

3.019 ± 0.026

10.239 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.521 ± 0.018

2.795 ± 0.029

5.152 ± 0.027

3.636 ± 0.019

6.871 ± 0.039

5.717 ± 0.03

5.78 ± 0.038

7.908 ± 0.028

1.385 ± 0.014

2.371 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski