Amino acid dipepetide frequency for Pandoraea sputorum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	16.42AlaAla: 16.42 ± 0.134	1.221AlaCys: 1.221 ± 0.028	6.364AlaAsp: 6.364 ± 0.068	5.6AlaGlu: 5.6 ± 0.08	4.278AlaPhe: 4.278 ± 0.053	10.397AlaGly: 10.397 ± 0.122	2.669AlaHis: 2.669 ± 0.042	5.71AlaIle: 5.71 ± 0.069	3.774AlaLys: 3.774 ± 0.057	14.041AlaLeu: 14.041 ± 0.116	3.635AlaMet: 3.635 ± 0.053	3.412AlaAsn: 3.412 ± 0.05	6.218AlaPro: 6.218 ± 0.081	5.695AlaGln: 5.695 ± 0.082	8.777AlaArg: 8.777 ± 0.081	7.769AlaSer: 7.769 ± 0.083	6.86AlaThr: 6.86 ± 0.071	8.832AlaVal: 8.832 ± 0.083	1.628AlaTrp: 1.628 ± 0.037	2.68AlaTyr: 2.68 ± 0.046	0.0AlaXaa: 0.0 ± 0.0
Cys	1.182CysAla: 1.182 ± 0.027	0.114CysCys: 0.114 ± 0.009	0.523CysAsp: 0.523 ± 0.019	0.479CysGlu: 0.479 ± 0.018	0.298CysPhe: 0.298 ± 0.013	0.963CysGly: 0.963 ± 0.028	0.262CysHis: 0.262 ± 0.012	0.361CysIle: 0.361 ± 0.014	0.186CysLys: 0.186 ± 0.011	0.808CysLeu: 0.808 ± 0.023	0.194CysMet: 0.194 ± 0.011	0.214CysAsn: 0.214 ± 0.012	0.441CysPro: 0.441 ± 0.017	0.243CysGln: 0.243 ± 0.013	0.568CysArg: 0.568 ± 0.02	0.445CysSer: 0.445 ± 0.018	0.471CysThr: 0.471 ± 0.018	0.793CysVal: 0.793 ± 0.023	0.105CysTrp: 0.105 ± 0.009	0.223CysTyr: 0.223 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	8.01AspAla: 8.01 ± 0.079	0.42AspCys: 0.42 ± 0.015	3.293AspAsp: 3.293 ± 0.051	3.171AspGlu: 3.171 ± 0.055	2.023AspPhe: 2.023 ± 0.041	4.768AspGly: 4.768 ± 0.059	1.049AspHis: 1.049 ± 0.028	2.718AspIle: 2.718 ± 0.041	1.695AspLys: 1.695 ± 0.033	5.023AspLeu: 5.023 ± 0.064	1.247AspMet: 1.247 ± 0.026	1.375AspAsn: 1.375 ± 0.032	2.775AspPro: 2.775 ± 0.049	1.444AspGln: 1.444 ± 0.029	3.125AspArg: 3.125 ± 0.046	2.296AspSer: 2.296 ± 0.04	3.005AspThr: 3.005 ± 0.045	4.764AspVal: 4.764 ± 0.062	0.914AspTrp: 0.914 ± 0.024	1.397AspTyr: 1.397 ± 0.035	0.0AspXaa: 0.0 ± 0.0
Glu	6.278GluAla: 6.278 ± 0.076	0.402GluCys: 0.402 ± 0.018	2.115GluAsp: 2.115 ± 0.045	2.016GluGlu: 2.016 ± 0.043	1.733GluPhe: 1.733 ± 0.034	3.332GluGly: 3.332 ± 0.045	1.283GluHis: 1.283 ± 0.027	2.72GluIle: 2.72 ± 0.043	1.734GluLys: 1.734 ± 0.035	4.773GluLeu: 4.773 ± 0.065	1.286GluMet: 1.286 ± 0.032	1.397GluAsn: 1.397 ± 0.029	2.12GluPro: 2.12 ± 0.041	2.176GluGln: 2.176 ± 0.041	4.582GluArg: 4.582 ± 0.067	2.485GluSer: 2.485 ± 0.039	2.846GluThr: 2.846 ± 0.045	3.558GluVal: 3.558 ± 0.052	0.689GluTrp: 0.689 ± 0.02	1.139GluTyr: 1.139 ± 0.026	0.0GluXaa: 0.0 ± 0.0
Phe	4.542PheAla: 4.542 ± 0.063	0.409PheCys: 0.409 ± 0.016	2.63PheAsp: 2.63 ± 0.044	1.887PheGlu: 1.887 ± 0.037	1.385PhePhe: 1.385 ± 0.034	3.788PheGly: 3.788 ± 0.054	0.758PheHis: 0.758 ± 0.022	1.506PheIle: 1.506 ± 0.033	0.953PheLys: 0.953 ± 0.026	2.991PheLeu: 2.991 ± 0.043	0.793PheMet: 0.793 ± 0.022	1.125PheAsn: 1.125 ± 0.03	1.536PhePro: 1.536 ± 0.033	0.974PheGln: 0.974 ± 0.024	1.909PheArg: 1.909 ± 0.037	2.264PheSer: 2.264 ± 0.039	2.052PheThr: 2.052 ± 0.036	3.108PheVal: 3.108 ± 0.048	0.54PheTrp: 0.54 ± 0.02	0.921PheTyr: 0.921 ± 0.026	0.0PheXaa: 0.0 ± 0.0
Gly	9.615GlyAla: 9.615 ± 0.125	0.787GlyCys: 0.787 ± 0.025	4.237GlyAsp: 4.237 ± 0.066	4.396GlyGlu: 4.396 ± 0.061	3.284GlyPhe: 3.284 ± 0.048	7.313GlyGly: 7.313 ± 0.174	1.865GlyHis: 1.865 ± 0.035	4.163GlyIle: 4.163 ± 0.057	3.33GlyLys: 3.33 ± 0.047	8.287GlyLeu: 8.287 ± 0.089	2.481GlyMet: 2.481 ± 0.038	2.621GlyAsn: 2.621 ± 0.068	2.945GlyPro: 2.945 ± 0.043	2.943GlyGln: 2.943 ± 0.043	5.197GlyArg: 5.197 ± 0.063	4.414GlySer: 4.414 ± 0.09	4.989GlyThr: 4.989 ± 0.114	7.157GlyVal: 7.157 ± 0.066	1.333GlyTrp: 1.333 ± 0.029	2.361GlyTyr: 2.361 ± 0.04	0.0GlyXaa: 0.0 ± 0.0
His	2.857HisAla: 2.857 ± 0.046	0.249HisCys: 0.249 ± 0.013	1.362HisAsp: 1.362 ± 0.033	1.095HisGlu: 1.095 ± 0.026	0.941HisPhe: 0.941 ± 0.025	2.068HisGly: 2.068 ± 0.041	0.605HisHis: 0.605 ± 0.024	0.963HisIle: 0.963 ± 0.026	0.508HisLys: 0.508 ± 0.017	2.128HisLeu: 2.128 ± 0.035	0.516HisMet: 0.516 ± 0.018	0.537HisAsn: 0.537 ± 0.017	1.389HisPro: 1.389 ± 0.034	0.634HisGln: 0.634 ± 0.019	1.536HisArg: 1.536 ± 0.034	1.056HisSer: 1.056 ± 0.025	1.065HisThr: 1.065 ± 0.022	1.758HisVal: 1.758 ± 0.035	0.408HisTrp: 0.408 ± 0.017	0.637HisTyr: 0.637 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	6.57IleAla: 6.57 ± 0.056	0.439IleCys: 0.439 ± 0.017	3.425IleAsp: 3.425 ± 0.055	2.96IleGlu: 2.96 ± 0.05	1.438IlePhe: 1.438 ± 0.037	4.659IleGly: 4.659 ± 0.059	0.865IleHis: 0.865 ± 0.024	1.54IleIle: 1.54 ± 0.039	1.348IleLys: 1.348 ± 0.037	3.502IleLeu: 3.502 ± 0.05	0.833IleMet: 0.833 ± 0.022	1.383IleAsn: 1.383 ± 0.037	2.101IlePro: 2.101 ± 0.035	1.132IleGln: 1.132 ± 0.025	2.701IleArg: 2.701 ± 0.041	2.572IleSer: 2.572 ± 0.045	2.586IleThr: 2.586 ± 0.045	4.331IleVal: 4.331 ± 0.07	0.489IleTrp: 0.489 ± 0.019	1.073IleTyr: 1.073 ± 0.026	0.0IleXaa: 0.0 ± 0.0
Lys	3.57LysAla: 3.57 ± 0.058	0.173LysCys: 0.173 ± 0.011	1.461LysAsp: 1.461 ± 0.036	1.281LysGlu: 1.281 ± 0.031	0.965LysPhe: 0.965 ± 0.027	2.152LysGly: 2.152 ± 0.041	0.701LysHis: 0.701 ± 0.021	1.443LysIle: 1.443 ± 0.029	1.152LysLys: 1.152 ± 0.039	3.259LysLeu: 3.259 ± 0.05	0.772LysMet: 0.772 ± 0.023	0.816LysAsn: 0.816 ± 0.026	1.868LysPro: 1.868 ± 0.042	1.258LysGln: 1.258 ± 0.029	2.326LysArg: 2.326 ± 0.045	1.734LysSer: 1.734 ± 0.035	1.878LysThr: 1.878 ± 0.034	2.3LysVal: 2.3 ± 0.038	0.352LysTrp: 0.352 ± 0.015	0.726LysTyr: 0.726 ± 0.022	0.0LysXaa: 0.0 ± 0.0
Leu	14.086LeuAla: 14.086 ± 0.122	1.033LeuCys: 1.033 ± 0.027	5.831LeuAsp: 5.831 ± 0.066	4.515LeuGlu: 4.515 ± 0.059	3.392LeuPhe: 3.392 ± 0.056	8.567LeuGly: 8.567 ± 0.094	2.176LeuHis: 2.176 ± 0.04	4.595LeuIle: 4.595 ± 0.062	3.044LeuLys: 3.044 ± 0.044	10.103LeuLeu: 10.103 ± 0.098	2.445LeuMet: 2.445 ± 0.036	2.677LeuAsn: 2.677 ± 0.045	5.969LeuPro: 5.969 ± 0.061	3.193LeuGln: 3.193 ± 0.042	7.281LeuArg: 7.281 ± 0.079	6.572LeuSer: 6.572 ± 0.078	6.183LeuThr: 6.183 ± 0.076	7.426LeuVal: 7.426 ± 0.081	1.241LeuTrp: 1.241 ± 0.031	2.057LeuTyr: 2.057 ± 0.037	0.0LeuXaa: 0.0 ± 0.0
Met	2.826MetAla: 2.826 ± 0.043	0.197MetCys: 0.197 ± 0.01	0.966MetAsp: 0.966 ± 0.027	0.928MetGlu: 0.928 ± 0.026	0.819MetPhe: 0.819 ± 0.021	1.763MetGly: 1.763 ± 0.032	0.575MetHis: 0.575 ± 0.019	1.122MetIle: 1.122 ± 0.025	0.893MetLys: 0.893 ± 0.025	2.854MetLeu: 2.854 ± 0.048	0.646MetMet: 0.646 ± 0.022	0.83MetAsn: 0.83 ± 0.022	1.634MetPro: 1.634 ± 0.032	1.066MetGln: 1.066 ± 0.028	1.939MetArg: 1.939 ± 0.035	1.879MetSer: 1.879 ± 0.031	1.931MetThr: 1.931 ± 0.037	1.621MetVal: 1.621 ± 0.037	0.251MetTrp: 0.251 ± 0.014	0.469MetTyr: 0.469 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	3.754AsnAla: 3.754 ± 0.071	0.229AsnCys: 0.229 ± 0.012	1.544AsnAsp: 1.544 ± 0.034	1.27AsnGlu: 1.27 ± 0.031	0.977AsnPhe: 0.977 ± 0.027	2.688AsnGly: 2.688 ± 0.063	0.504AsnHis: 0.504 ± 0.018	1.283AsnIle: 1.283 ± 0.031	0.765AsnLys: 0.765 ± 0.024	2.698AsnLeu: 2.698 ± 0.041	0.565AsnMet: 0.565 ± 0.016	0.846AsnAsn: 0.846 ± 0.031	1.753AsnPro: 1.753 ± 0.033	0.846AsnGln: 0.846 ± 0.022	1.773AsnArg: 1.773 ± 0.037	1.301AsnSer: 1.301 ± 0.034	1.606AsnThr: 1.606 ± 0.045	2.448AsnVal: 2.448 ± 0.05	0.39AsnTrp: 0.39 ± 0.016	0.707AsnTyr: 0.707 ± 0.022	0.0AsnXaa: 0.0 ± 0.0
Pro	6.367ProAla: 6.367 ± 0.073	0.335ProCys: 0.335 ± 0.016	3.258ProAsp: 3.258 ± 0.049	3.019ProGlu: 3.019 ± 0.044	1.858ProPhe: 1.858 ± 0.033	4.325ProGly: 4.325 ± 0.065	1.16ProHis: 1.16 ± 0.028	2.041ProIle: 2.041 ± 0.036	1.5ProLys: 1.5 ± 0.032	5.033ProLeu: 5.033 ± 0.06	1.295ProMet: 1.295 ± 0.029	1.519ProAsn: 1.519 ± 0.032	2.561ProPro: 2.561 ± 0.051	1.939ProGln: 1.939 ± 0.038	2.842ProArg: 2.842 ± 0.045	3.148ProSer: 3.148 ± 0.046	2.886ProThr: 2.886 ± 0.036	4.488ProVal: 4.488 ± 0.056	0.721ProTrp: 0.721 ± 0.023	1.227ProTyr: 1.227 ± 0.029	0.0ProXaa: 0.0 ± 0.0
Gln	4.745GlnAla: 4.745 ± 0.065	0.288GlnCys: 0.288 ± 0.013	1.475GlnAsp: 1.475 ± 0.028	1.283GlnGlu: 1.283 ± 0.029	1.244GlnPhe: 1.244 ± 0.029	2.73GlnGly: 2.73 ± 0.044	0.864GlnHis: 0.864 ± 0.029	1.952GlnIle: 1.952 ± 0.036	1.032GlnLys: 1.032 ± 0.026	3.677GlnLeu: 3.677 ± 0.045	1.012GlnMet: 1.012 ± 0.026	0.973GlnAsn: 0.973 ± 0.024	1.86GlnPro: 1.86 ± 0.036	1.637GlnGln: 1.637 ± 0.042	3.029GlnArg: 3.029 ± 0.05	2.141GlnSer: 2.141 ± 0.041	2.293GlnThr: 2.293 ± 0.037	2.573GlnVal: 2.573 ± 0.04	0.642GlnTrp: 0.642 ± 0.02	0.91GlnTyr: 0.91 ± 0.025	0.0GlnXaa: 0.0 ± 0.0
Arg	7.984ArgAla: 7.984 ± 0.078	0.555ArgCys: 0.555 ± 0.019	4.035ArgAsp: 4.035 ± 0.055	4.316ArgGlu: 4.316 ± 0.064	2.803ArgPhe: 2.803 ± 0.044	4.522ArgGly: 4.522 ± 0.063	1.92ArgHis: 1.92 ± 0.04	3.487ArgIle: 3.487 ± 0.045	1.938ArgLys: 1.938 ± 0.038	7.249ArgLeu: 7.249 ± 0.071	1.883ArgMet: 1.883 ± 0.036	1.917ArgAsn: 1.917 ± 0.041	2.99ArgPro: 2.99 ± 0.043	2.687ArgGln: 2.687 ± 0.045	5.248ArgArg: 5.248 ± 0.069	3.311ArgSer: 3.311 ± 0.047	3.534ArgThr: 3.534 ± 0.049	5.331ArgVal: 5.331 ± 0.066	1.102ArgTrp: 1.102 ± 0.024	1.892ArgTyr: 1.892 ± 0.029	0.0ArgXaa: 0.0 ± 0.0
Ser	7.115SerAla: 7.115 ± 0.077	0.393SerCys: 0.393 ± 0.017	2.929SerAsp: 2.929 ± 0.048	2.542SerGlu: 2.542 ± 0.043	2.045SerPhe: 2.045 ± 0.037	5.396SerGly: 5.396 ± 0.1	1.322SerHis: 1.322 ± 0.03	2.522SerIle: 2.522 ± 0.045	1.518SerLys: 1.518 ± 0.035	6.154SerLeu: 6.154 ± 0.065	1.433SerMet: 1.433 ± 0.029	1.56SerAsn: 1.56 ± 0.038	3.262SerPro: 3.262 ± 0.047	2.017SerGln: 2.017 ± 0.036	3.769SerArg: 3.769 ± 0.049	3.373SerSer: 3.373 ± 0.059	3.3SerThr: 3.3 ± 0.056	4.509SerVal: 4.509 ± 0.056	0.697SerTrp: 0.697 ± 0.021	1.311SerTyr: 1.311 ± 0.031	0.0SerXaa: 0.0 ± 0.0
Thr	6.196ThrAla: 6.196 ± 0.085	0.449ThrCys: 0.449 ± 0.017	2.84ThrAsp: 2.84 ± 0.039	2.391ThrGlu: 2.391 ± 0.043	2.208ThrPhe: 2.208 ± 0.043	5.101ThrGly: 5.101 ± 0.088	1.307ThrHis: 1.307 ± 0.026	2.515ThrIle: 2.515 ± 0.045	1.261ThrLys: 1.261 ± 0.036	7.167ThrLeu: 7.167 ± 0.106	1.273ThrMet: 1.273 ± 0.028	1.464ThrAsn: 1.464 ± 0.037	4.013ThrPro: 4.013 ± 0.058	2.277ThrGln: 2.277 ± 0.04	3.741ThrArg: 3.741 ± 0.048	3.513ThrSer: 3.513 ± 0.062	3.304ThrThr: 3.304 ± 0.061	4.697ThrVal: 4.697 ± 0.069	0.726ThrTrp: 0.726 ± 0.022	1.355ThrTyr: 1.355 ± 0.029	0.0ThrXaa: 0.0 ± 0.0
Val	9.912ValAla: 9.912 ± 0.094	0.801ValCys: 0.801 ± 0.023	4.332ValAsp: 4.332 ± 0.063	3.855ValGlu: 3.855 ± 0.049	2.832ValPhe: 2.832 ± 0.045	6.016ValGly: 6.016 ± 0.068	1.499ValHis: 1.499 ± 0.03	3.797ValIle: 3.797 ± 0.054	2.567ValLys: 2.567 ± 0.047	8.027ValLeu: 8.027 ± 0.086	2.07ValMet: 2.07 ± 0.041	2.262ValAsn: 2.262 ± 0.048	4.243ValPro: 4.243 ± 0.053	2.577ValGln: 2.577 ± 0.039	5.145ValArg: 5.145 ± 0.067	4.905ValSer: 4.905 ± 0.061	5.034ValThr: 5.034 ± 0.069	6.548ValVal: 6.548 ± 0.076	1.018ValTrp: 1.018 ± 0.023	1.647ValTyr: 1.647 ± 0.035	0.0ValXaa: 0.0 ± 0.0
Trp	1.167TrpAla: 1.167 ± 0.027	0.147TrpCys: 0.147 ± 0.01	0.555TrpAsp: 0.555 ± 0.016	0.531TrpGlu: 0.531 ± 0.02	0.534TrpPhe: 0.534 ± 0.021	0.916TrpGly: 0.916 ± 0.026	0.425TrpHis: 0.425 ± 0.017	0.7TrpIle: 0.7 ± 0.019	0.354TrpLys: 0.354 ± 0.016	2.053TrpLeu: 2.053 ± 0.042	0.395TrpMet: 0.395 ± 0.017	0.384TrpAsn: 0.384 ± 0.015	0.661TrpPro: 0.661 ± 0.02	0.765TrpGln: 0.765 ± 0.024	1.321TrpArg: 1.321 ± 0.031	0.74TrpSer: 0.74 ± 0.019	0.693TrpThr: 0.693 ± 0.02	0.969TrpVal: 0.969 ± 0.023	0.215TrpTrp: 0.215 ± 0.011	0.322TrpTyr: 0.322 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.787TyrAla: 2.787 ± 0.04	0.232TyrCys: 0.232 ± 0.013	1.336TyrAsp: 1.336 ± 0.029	1.13TyrGlu: 1.13 ± 0.029	1.016TyrPhe: 1.016 ± 0.025	2.256TyrGly: 2.256 ± 0.044	0.5TyrHis: 0.5 ± 0.019	0.812TyrIle: 0.812 ± 0.025	0.634TyrLys: 0.634 ± 0.02	2.56TyrLeu: 2.56 ± 0.043	0.436TyrMet: 0.436 ± 0.017	0.634TyrAsn: 0.634 ± 0.021	1.184TyrPro: 1.184 ± 0.033	0.856TyrGln: 0.856 ± 0.028	1.895TyrArg: 1.895 ± 0.038	1.249TyrSer: 1.249 ± 0.03	1.28TyrThr: 1.28 ± 0.03	1.872TyrVal: 1.872 ± 0.032	0.384TyrTrp: 0.384 ± 0.017	0.656TyrTyr: 0.656 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5005 proteins (1675428 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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