Xanthomonas phage Xp12
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 82 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A7G9UT20|A0A7G9UT20_9CAUD Uncharacterized protein OS=Xanthomonas phage Xp12 OX=2746072 PE=4 SV=1
MM1 pKa = 7.6 AAFSCNVCGQGFDD14 pKa = 5.32 FGDD17 pKa = 4.3 CRR19 pKa = 11.84 CDD21 pKa = 3.84 SLPDD25 pKa = 3.92 PQPSGGLPSVEE36 pKa = 3.98 ALAEE40 pKa = 4.02 AFVRR44 pKa = 11.84 LLRR47 pKa = 11.84 MNLGQDD53 pKa = 3.28 VYY55 pKa = 11.32 DD56 pKa = 3.81 TVVDD60 pKa = 4.49 LNSRR64 pKa = 11.84 EE65 pKa = 4.21 LNPDD69 pKa = 3.01 VCHH72 pKa = 7.13 SGDD75 pKa = 3.52 FCDD78 pKa = 5.01 SNMVMDD84 pKa = 5.02 AAFGEE89 pKa = 4.53 LGVDD93 pKa = 3.72 PSTYY97 pKa = 10.34 GRR99 pKa = 11.84 EE100 pKa = 4.15 DD101 pKa = 3.77 EE102 pKa = 4.92 DD103 pKa = 5.73 DD104 pKa = 4.16 GMPQEE109 pKa = 5.99 VCDD112 pKa = 4.38 LWNSAWDD119 pKa = 3.71 RR120 pKa = 11.84 AKK122 pKa = 11.18 VLMQEE127 pKa = 3.95 RR128 pKa = 11.84 RR129 pKa = 11.84 TPP131 pKa = 3.24
Molecular weight: 14.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.927
IPC2_protein 3.859
IPC_protein 3.859
Toseland 3.63
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.808
Rodwell 3.681
Grimsley 3.541
Solomon 3.846
Lehninger 3.795
Nozaki 3.973
DTASelect 4.228
Thurlkill 3.694
EMBOSS 3.808
Sillero 3.973
Patrickios 2.804
IPC_peptide 3.834
IPC2_peptide 3.948
IPC2.peptide.svr19 3.897
Protein with the highest isoelectric point:
>tr|A0A7G9UT56|A0A7G9UT56_9CAUD Uncharacterized protein OS=Xanthomonas phage Xp12 OX=2746072 PE=4 SV=1
MM1 pKa = 7.87 PNTARR6 pKa = 11.84 TIKK9 pKa = 10.75 EE10 pKa = 3.86 EE11 pKa = 3.79 VRR13 pKa = 11.84 VLGRR17 pKa = 11.84 RR18 pKa = 11.84 KK19 pKa = 9.72 RR20 pKa = 11.84 ALTYY24 pKa = 9.93 PAMVDD29 pKa = 3.54 IIKK32 pKa = 10.42 ARR34 pKa = 11.84 HH35 pKa = 5.83 PEE37 pKa = 3.89 ANTSVKK43 pKa = 8.91 TVQWYY48 pKa = 10.09 ASRR51 pKa = 11.84 LRR53 pKa = 11.84 RR54 pKa = 11.84 DD55 pKa = 3.63 GEE57 pKa = 4.22 DD58 pKa = 3.26 VNVKK62 pKa = 10.42 DD63 pKa = 4.51 GRR65 pKa = 11.84 TASKK69 pKa = 8.95 PTVHH73 pKa = 6.93
Molecular weight: 8.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.314
IPC2_protein 9.736
IPC_protein 10.57
Toseland 10.891
ProMoST 10.599
Dawson 10.95
Bjellqvist 10.657
Wikipedia 11.169
Rodwell 11.169
Grimsley 10.994
Solomon 11.111
Lehninger 11.082
Nozaki 10.847
DTASelect 10.657
Thurlkill 10.877
EMBOSS 11.286
Sillero 10.891
Patrickios 10.935
IPC_peptide 11.111
IPC2_peptide 9.399
IPC2.peptide.svr19 8.689
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
82
0
82
19533
40
1192
238.2
26.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.731 ± 0.338
0.962 ± 0.124
6.481 ± 0.238
6.517 ± 0.333
3.466 ± 0.172
8.273 ± 0.258
1.904 ± 0.151
3.415 ± 0.173
4.592 ± 0.422
8.642 ± 0.179
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.637 ± 0.13
2.974 ± 0.134
5.207 ± 0.267
4.121 ± 0.2
6.896 ± 0.283
5.908 ± 0.315
5.575 ± 0.253
6.998 ± 0.193
1.93 ± 0.149
2.77 ± 0.193
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here