Listeria phage A511 (Bacteriophage A511)
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 190 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A8AT51|A8AT51_BPA51 Gp27 OS=Listeria phage A511 OX=40523 PE=4 SV=1
MM1 pKa = 8.16 DD2 pKa = 4.63 SNKK5 pKa = 9.97 FLMGLNKK12 pKa = 9.86 FLTEE16 pKa = 3.74 IDD18 pKa = 4.21 KK19 pKa = 9.39 VTSAYY24 pKa = 10.55 GEE26 pKa = 4.07 DD27 pKa = 3.4 ANYY30 pKa = 9.69 EE31 pKa = 3.62 ISFIRR36 pKa = 11.84 EE37 pKa = 3.29 KK38 pKa = 11.03 CADD41 pKa = 3.99 LDD43 pKa = 3.87 EE44 pKa = 5.07 GAKK47 pKa = 9.62 QDD49 pKa = 4.56 CEE51 pKa = 3.97 EE52 pKa = 4.06 TDD54 pKa = 3.78 FFEE57 pKa = 4.34 GAEE60 pKa = 4.68 YY61 pKa = 9.94 ISQMQTGEE69 pKa = 4.24 DD70 pKa = 3.72 CFEE73 pKa = 4.16 GYY75 pKa = 9.85 ILRR78 pKa = 11.84 KK79 pKa = 9.45 IKK81 pKa = 9.58 NTDD84 pKa = 2.4 WCLCITYY91 pKa = 10.4 AII93 pKa = 4.41
Molecular weight: 10.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.883
IPC2_protein 4.164
IPC_protein 4.075
Toseland 3.897
ProMoST 4.215
Dawson 4.037
Bjellqvist 4.19
Wikipedia 3.935
Rodwell 3.91
Grimsley 3.808
Solomon 4.037
Lehninger 3.986
Nozaki 4.151
DTASelect 4.317
Thurlkill 3.923
EMBOSS 3.948
Sillero 4.19
Patrickios 1.939
IPC_peptide 4.037
IPC2_peptide 4.177
IPC2.peptide.svr19 4.088
Protein with the highest isoelectric point:
>tr|A8AT88|A8AT88_BPA51 Gp64 OS=Listeria phage A511 OX=40523 PE=4 SV=1
MM1 pKa = 7.95 CIRR4 pKa = 11.84 YY5 pKa = 7.64 TIRR8 pKa = 11.84 TRR10 pKa = 11.84 FSYY13 pKa = 8.94 THH15 pKa = 6.58 SPALNQMRR23 pKa = 11.84 RR24 pKa = 11.84 NGHH27 pKa = 5.29 RR28 pKa = 11.84 SNRR31 pKa = 11.84 KK32 pKa = 8.33 GVEE35 pKa = 4.02 PFPSCAKK42 pKa = 9.74 EE43 pKa = 4.14 SNIGLLLCYY52 pKa = 10.18 LVV54 pKa = 3.89
Molecular weight: 6.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.316
IPC2_protein 9.677
IPC_protein 10.452
Toseland 10.131
ProMoST 10.014
Dawson 10.394
Bjellqvist 10.204
Wikipedia 10.643
Rodwell 10.511
Grimsley 10.496
Solomon 10.467
Lehninger 10.423
Nozaki 10.262
DTASelect 10.16
Thurlkill 10.233
EMBOSS 10.555
Sillero 10.335
Patrickios 10.248
IPC_peptide 10.467
IPC2_peptide 9.619
IPC2.peptide.svr19 8.264
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
190
0
190
39955
31
1309
210.3
23.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.721 ± 0.211
0.673 ± 0.055
5.892 ± 0.13
8.174 ± 0.281
3.979 ± 0.12
5.889 ± 0.273
1.497 ± 0.087
6.597 ± 0.185
8.667 ± 0.254
8.6 ± 0.195
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.46 ± 0.076
5.659 ± 0.138
2.851 ± 0.167
3.324 ± 0.214
4.16 ± 0.127
6.835 ± 0.157
6.275 ± 0.173
7.278 ± 0.19
0.954 ± 0.058
4.515 ± 0.129
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here