Enterococcus dispar ATCC 51266
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2622 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S1P060|S1P060_9ENTE HTH_OrfB_IS605 domain-containing protein OS=Enterococcus dispar ATCC 51266 OX=1139219 GN=OMK_02085 PE=4 SV=1
MM1 pKa = 7.51 TEE3 pKa = 4.22 HH4 pKa = 6.46 NHH6 pKa = 6.09 DD7 pKa = 4.47 HH8 pKa = 5.93 NHH10 pKa = 5.54 DD11 pKa = 4.18 HH12 pKa = 7.06 EE13 pKa = 4.56 GHH15 pKa = 5.12 EE16 pKa = 4.96 HH17 pKa = 5.6 ITLVDD22 pKa = 3.67 DD23 pKa = 4.08 QGNEE27 pKa = 3.72 TLYY30 pKa = 10.95 EE31 pKa = 3.99 ILLTIDD37 pKa = 3.18 GQEE40 pKa = 3.91 EE41 pKa = 4.25 FKK43 pKa = 11.01 RR44 pKa = 11.84 NYY46 pKa = 9.55 VLLYY50 pKa = 9.58 PAGASEE56 pKa = 4.9 DD57 pKa = 4.08 DD58 pKa = 4.13 DD59 pKa = 5.82 VEE61 pKa = 4.28 LQAYY65 pKa = 9.81 AYY67 pKa = 9.68 IEE69 pKa = 4.46 NEE71 pKa = 4.25 DD72 pKa = 3.63 GTEE75 pKa = 4.16 GEE77 pKa = 4.42 LEE79 pKa = 4.17 QIEE82 pKa = 4.85 TEE84 pKa = 4.52 AEE86 pKa = 3.15 WDD88 pKa = 3.58 MIEE91 pKa = 4.45 EE92 pKa = 4.22 VFNTFMAEE100 pKa = 3.84 EE101 pKa = 4.31 EE102 pKa = 4.32 EE103 pKa = 4.38
Molecular weight: 12.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.778
IPC2_protein 3.859
IPC_protein 3.795
Toseland 3.63
ProMoST 3.897
Dawson 3.745
Bjellqvist 3.935
Wikipedia 3.617
Rodwell 3.63
Grimsley 3.541
Solomon 3.732
Lehninger 3.694
Nozaki 3.872
DTASelect 3.986
Thurlkill 3.656
EMBOSS 3.643
Sillero 3.91
Patrickios 0.693
IPC_peptide 3.745
IPC2_peptide 3.897
IPC2.peptide.svr19 3.813
Protein with the highest isoelectric point:
>tr|S1NC85|S1NC85_9ENTE Metallophos domain-containing protein OS=Enterococcus dispar ATCC 51266 OX=1139219 GN=OMK_01843 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.34 QPNKK9 pKa = 9.16 RR10 pKa = 11.84 KK11 pKa = 9.12 RR12 pKa = 11.84 QKK14 pKa = 9.38 VHH16 pKa = 5.8 GFRR19 pKa = 11.84 KK20 pKa = 10.04 RR21 pKa = 11.84 MSTKK25 pKa = 9.46 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLASRR34 pKa = 11.84 RR35 pKa = 11.84 LKK37 pKa = 10.13 GRR39 pKa = 11.84 KK40 pKa = 8.79 VLSAA44 pKa = 4.05
Molecular weight: 5.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.415
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.398
Grimsley 12.661
Solomon 13.115
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.135
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.013
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2622
0
2622
795991
29
2138
303.6
34.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.807 ± 0.051
0.61 ± 0.012
5.369 ± 0.04
6.894 ± 0.057
4.641 ± 0.042
6.586 ± 0.041
1.782 ± 0.022
7.398 ± 0.048
7.185 ± 0.045
9.862 ± 0.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.63 ± 0.024
4.827 ± 0.039
3.457 ± 0.022
4.264 ± 0.033
3.694 ± 0.031
5.547 ± 0.039
5.954 ± 0.045
6.872 ± 0.041
0.938 ± 0.017
3.683 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here