Mycobacterium sp. Root135

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Mycobacterium; unclassified Mycobacterium

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5995 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0T1WGB6|A0A0T1WGB6_9MYCO Uncharacterized protein OS=Mycobacterium sp. Root135 OX=1736457 GN=ASD37_09440 PE=4 SV=1
MM1 pKa = 6.86PTKK4 pKa = 10.44LAAAFLAAGVISTGALVAPDD24 pKa = 4.35KK25 pKa = 11.44VPLPVVSADD34 pKa = 3.31VANASVITDD43 pKa = 3.69ALYY46 pKa = 10.91RR47 pKa = 11.84LGDD50 pKa = 3.85VVNGAAYY57 pKa = 9.84GYY59 pKa = 10.71AITQDD64 pKa = 3.84AGSSLPFDD72 pKa = 3.53VATAFTIAAQNPTLAPSLLSWLVNRR97 pKa = 11.84YY98 pKa = 9.32ANPSYY103 pKa = 11.07DD104 pKa = 3.13YY105 pKa = 10.61GYY107 pKa = 9.41PSEE110 pKa = 4.88SGYY113 pKa = 8.67FTYY116 pKa = 8.49PTYY119 pKa = 10.47FRR121 pKa = 11.84EE122 pKa = 4.04YY123 pKa = 9.1SLEE126 pKa = 4.32VIAGALPFPLGPAGANPGLINSAANAIGQAVGGFLSGVLPNPAGGLAATDD176 pKa = 4.13AFWATDD182 pKa = 2.97IGKK185 pKa = 8.71TIVAANLAVTAPVWAFYY202 pKa = 9.71STAFYY207 pKa = 10.63LGYY210 pKa = 10.69LPADD214 pKa = 3.92LEE216 pKa = 4.55ATFEE220 pKa = 4.29SAIQNPTEE228 pKa = 4.14IPGLLSNLVYY238 pKa = 10.92DD239 pKa = 4.91LVASDD244 pKa = 4.67GLLGSLIQDD253 pKa = 3.57LSAPLRR259 pKa = 11.84ALPGPIGVLAQNVVASLNAGIDD281 pKa = 3.54NLLSVLPAPIEE292 pKa = 3.95PTPFPSATSNVNRR305 pKa = 11.84VADD308 pKa = 4.41TSAEE312 pKa = 4.03RR313 pKa = 11.84QVTSIPDD320 pKa = 3.46PSITMDD326 pKa = 3.65NVVTLNTPVKK336 pKa = 10.11LDD338 pKa = 3.56NPVAPSSDD346 pKa = 3.78SPPPNAGGTTTGLVKK361 pKa = 10.27TNDD364 pKa = 3.46SGPQLNVVRR373 pKa = 11.84DD374 pKa = 4.1SVKK377 pKa = 9.38VTPGDD382 pKa = 3.42TFAGVTTGTGTGTGTEE398 pKa = 4.41TEE400 pKa = 4.31SATTVDD406 pKa = 3.87EE407 pKa = 4.59TVSGATTASPAAANDD422 pKa = 3.92PSDD425 pKa = 3.38AAAGEE430 pKa = 4.31NVSPSSGAGDD440 pKa = 3.73PGAAAA445 pKa = 4.58

Molecular weight: 44.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0T1WG68|A0A0T1WG68_9MYCO Aminotransferase class V OS=Mycobacterium sp. Root135 OX=1736457 GN=ASD37_09005 PE=4 SV=1
MM1 pKa = 7.41SRR3 pKa = 11.84PEE5 pKa = 3.94GQPARR10 pKa = 11.84GLRR13 pKa = 11.84ILPSATTVLAICAGLTSIKK32 pKa = 10.15FALDD36 pKa = 3.33EE37 pKa = 4.66KK38 pKa = 10.52PWISLALIGAAAILDD53 pKa = 4.84GIDD56 pKa = 3.22GGIARR61 pKa = 11.84ALNAQSRR68 pKa = 11.84MGAEE72 pKa = 3.94IDD74 pKa = 3.88SLADD78 pKa = 3.28AVNFGVAPALVVYY91 pKa = 8.04LTLLPNSQLGWIFVLLYY108 pKa = 10.08AVCVVLRR115 pKa = 11.84LARR118 pKa = 11.84FNALLDD124 pKa = 3.85DD125 pKa = 4.12HH126 pKa = 6.93TKK128 pKa = 9.84PAYY131 pKa = 9.06TRR133 pKa = 11.84EE134 pKa = 4.06FFTGVPAPCGAVGALGPLVAFMQFGHH160 pKa = 6.17GWWTSHH166 pKa = 5.63WFVCAWLAANAALLVSRR183 pKa = 11.84VPTLALKK190 pKa = 10.53AISVPEE196 pKa = 3.78NAAPILLILVAAAAAGLLLFPYY218 pKa = 10.43VLVLLIIVGYY228 pKa = 9.07VCMMPFTIRR237 pKa = 11.84SQRR240 pKa = 11.84WVAARR245 pKa = 11.84PEE247 pKa = 3.94AWDD250 pKa = 3.56AKK252 pKa = 9.06PGQRR256 pKa = 11.84RR257 pKa = 11.84AVRR260 pKa = 11.84RR261 pKa = 11.84AARR264 pKa = 11.84RR265 pKa = 11.84AAPNRR270 pKa = 11.84RR271 pKa = 11.84SVARR275 pKa = 11.84LGLRR279 pKa = 11.84KK280 pKa = 9.59PGSRR284 pKa = 11.84GG285 pKa = 3.17

Molecular weight: 30.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5995

0

5995

1952207

31

8621

325.6

34.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.946 ± 0.04

0.764 ± 0.009

6.523 ± 0.027

5.106 ± 0.025

3.116 ± 0.019

8.988 ± 0.033

2.149 ± 0.016

4.01 ± 0.02

2.209 ± 0.019

9.823 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.058 ± 0.013

2.223 ± 0.017

5.647 ± 0.029

2.816 ± 0.016

6.906 ± 0.031

5.547 ± 0.018

6.271 ± 0.023

9.321 ± 0.032

1.476 ± 0.015

2.099 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski