Amino acid dipepetide frequency for Mycobacterium sp. Root135

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.432AlaAla: 19.432 ± 0.142	0.978AlaCys: 0.978 ± 0.023	8.698AlaAsp: 8.698 ± 0.065	7.454AlaGlu: 7.454 ± 0.077	3.732AlaPhe: 3.732 ± 0.045	11.456AlaGly: 11.456 ± 0.092	2.471AlaHis: 2.471 ± 0.039	5.264AlaIle: 5.264 ± 0.053	3.163AlaLys: 3.163 ± 0.048	13.006AlaLeu: 13.006 ± 0.108	2.952AlaMet: 2.952 ± 0.039	2.751AlaAsn: 2.751 ± 0.039	6.161AlaPro: 6.161 ± 0.082	3.547AlaGln: 3.547 ± 0.046	8.031AlaArg: 8.031 ± 0.064	6.454AlaSer: 6.454 ± 0.064	7.702AlaThr: 7.702 ± 0.059	12.176AlaVal: 12.176 ± 0.097	1.648AlaTrp: 1.648 ± 0.03	2.386AlaTyr: 2.386 ± 0.038	0.001AlaXaa: 0.001 ± 0.001
Cys	1.021CysAla: 1.021 ± 0.023	0.081CysCys: 0.081 ± 0.006	0.508CysAsp: 0.508 ± 0.018	0.367CysGlu: 0.367 ± 0.014	0.21CysPhe: 0.21 ± 0.01	0.897CysGly: 0.897 ± 0.024	0.195CysHis: 0.195 ± 0.011	0.274CysIle: 0.274 ± 0.011	0.124CysLys: 0.124 ± 0.007	0.629CysLeu: 0.629 ± 0.017	0.118CysMet: 0.118 ± 0.008	0.16CysAsn: 0.16 ± 0.01	0.442CysPro: 0.442 ± 0.019	0.199CysGln: 0.199 ± 0.01	0.533CysArg: 0.533 ± 0.016	0.459CysSer: 0.459 ± 0.016	0.456CysThr: 0.456 ± 0.015	0.664CysVal: 0.664 ± 0.019	0.123CysTrp: 0.123 ± 0.008	0.177CysTyr: 0.177 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	8.371AspAla: 8.371 ± 0.076	0.433AspCys: 0.433 ± 0.015	4.934AspAsp: 4.934 ± 0.062	4.15AspGlu: 4.15 ± 0.046	1.91AspPhe: 1.91 ± 0.028	6.604AspGly: 6.604 ± 0.068	1.452AspHis: 1.452 ± 0.03	2.068AspIle: 2.068 ± 0.038	1.268AspLys: 1.268 ± 0.027	6.219AspLeu: 6.219 ± 0.066	1.073AspMet: 1.073 ± 0.023	1.092AspAsn: 1.092 ± 0.023	4.267AspPro: 4.267 ± 0.045	1.821AspGln: 1.821 ± 0.032	4.56AspArg: 4.56 ± 0.049	2.831AspSer: 2.831 ± 0.04	3.099AspThr: 3.099 ± 0.038	6.685AspVal: 6.685 ± 0.073	0.973AspTrp: 0.973 ± 0.023	1.418AspTyr: 1.418 ± 0.027	0.001AspXaa: 0.001 ± 0.001
Glu	5.993GluAla: 5.993 ± 0.061	0.346GluCys: 0.346 ± 0.015	2.495GluAsp: 2.495 ± 0.039	2.342GluGlu: 2.342 ± 0.038	1.783GluPhe: 1.783 ± 0.033	3.35GluGly: 3.35 ± 0.043	1.427GluHis: 1.427 ± 0.027	2.391GluIle: 2.391 ± 0.037	1.302GluLys: 1.302 ± 0.035	6.327GluLeu: 6.327 ± 0.062	1.1GluMet: 1.1 ± 0.023	1.12GluAsn: 1.12 ± 0.027	2.948GluPro: 2.948 ± 0.042	1.916GluGln: 1.916 ± 0.034	4.37GluArg: 4.37 ± 0.06	2.826GluSer: 2.826 ± 0.042	2.752GluThr: 2.752 ± 0.039	4.446GluVal: 4.446 ± 0.059	0.739GluTrp: 0.739 ± 0.02	1.089GluTyr: 1.089 ± 0.026	0.001GluXaa: 0.001 ± 0.001
Phe	4.101PheAla: 4.101 ± 0.053	0.298PheCys: 0.298 ± 0.013	2.588PheAsp: 2.588 ± 0.038	1.562PheGlu: 1.562 ± 0.027	1.045PhePhe: 1.045 ± 0.03	3.654PheGly: 3.654 ± 0.048	0.643PheHis: 0.643 ± 0.022	1.057PheIle: 1.057 ± 0.027	0.542PheLys: 0.542 ± 0.019	2.668PheLeu: 2.668 ± 0.038	0.506PheMet: 0.506 ± 0.016	0.783PheAsn: 0.783 ± 0.018	1.423PhePro: 1.423 ± 0.029	0.687PheGln: 0.687 ± 0.018	1.642PheArg: 1.642 ± 0.029	1.697PheSer: 1.697 ± 0.034	2.209PheThr: 2.209 ± 0.031	2.897PheVal: 2.897 ± 0.041	0.458PheTrp: 0.458 ± 0.019	0.7PheTyr: 0.7 ± 0.02	0.0PheXaa: 0.0 ± 0.0
Gly	10.283GlyAla: 10.283 ± 0.101	0.779GlyCys: 0.779 ± 0.019	5.315GlyAsp: 5.315 ± 0.057	4.287GlyGlu: 4.287 ± 0.051	3.139GlyPhe: 3.139 ± 0.045	8.058GlyGly: 8.058 ± 0.128	2.046GlyHis: 2.046 ± 0.035	4.033GlyIle: 4.033 ± 0.047	2.372GlyLys: 2.372 ± 0.04	8.86GlyLeu: 8.86 ± 0.077	2.149GlyMet: 2.149 ± 0.038	2.039GlyAsn: 2.039 ± 0.045	4.371GlyPro: 4.371 ± 0.057	2.767GlyGln: 2.767 ± 0.047	6.055GlyArg: 6.055 ± 0.065	5.39GlySer: 5.39 ± 0.057	5.562GlyThr: 5.562 ± 0.06	8.337GlyVal: 8.337 ± 0.071	1.661GlyTrp: 1.661 ± 0.029	2.371GlyTyr: 2.371 ± 0.04	0.002GlyXaa: 0.002 ± 0.001
His	2.399HisAla: 2.399 ± 0.036	0.191HisCys: 0.191 ± 0.01	1.498HisAsp: 1.498 ± 0.034	1.042HisGlu: 1.042 ± 0.023	0.681HisPhe: 0.681 ± 0.021	2.122HisGly: 2.122 ± 0.034	0.666HisHis: 0.666 ± 0.021	0.632HisIle: 0.632 ± 0.019	0.295HisLys: 0.295 ± 0.012	2.192HisLeu: 2.192 ± 0.038	0.364HisMet: 0.364 ± 0.014	0.41HisAsn: 0.41 ± 0.016	1.612HisPro: 1.612 ± 0.031	0.65HisGln: 0.65 ± 0.017	1.729HisArg: 1.729 ± 0.033	1.032HisSer: 1.032 ± 0.023	1.151HisThr: 1.151 ± 0.026	1.953HisVal: 1.953 ± 0.033	0.336HisTrp: 0.336 ± 0.015	0.54HisTyr: 0.54 ± 0.017	0.0HisXaa: 0.0 ± 0.0
Ile	6.168IleAla: 6.168 ± 0.06	0.371IleCys: 0.371 ± 0.014	2.62IleAsp: 2.62 ± 0.039	2.153IleGlu: 2.153 ± 0.035	1.101IlePhe: 1.101 ± 0.027	4.554IleGly: 4.554 ± 0.046	0.742IleHis: 0.742 ± 0.02	1.27IleIle: 1.27 ± 0.03	0.818IleLys: 0.818 ± 0.023	3.1IleLeu: 3.1 ± 0.044	0.594IleMet: 0.594 ± 0.018	0.921IleAsn: 0.921 ± 0.025	2.247IlePro: 2.247 ± 0.035	0.907IleGln: 0.907 ± 0.026	2.533IleArg: 2.533 ± 0.034	2.268IleSer: 2.268 ± 0.032	2.619IleThr: 2.619 ± 0.043	3.95IleVal: 3.95 ± 0.056	0.459IleTrp: 0.459 ± 0.014	0.708IleTyr: 0.708 ± 0.018	0.001IleXaa: 0.001 ± 0.0
Lys	2.745LysAla: 2.745 ± 0.043	0.123LysCys: 0.123 ± 0.008	1.222LysAsp: 1.222 ± 0.03	0.91LysGlu: 0.91 ± 0.026	0.639LysPhe: 0.639 ± 0.015	1.66LysGly: 1.66 ± 0.031	0.48LysHis: 0.48 ± 0.017	1.037LysIle: 1.037 ± 0.023	0.709LysLys: 0.709 ± 0.026	2.21LysLeu: 2.21 ± 0.04	0.49LysMet: 0.49 ± 0.015	0.543LysAsn: 0.543 ± 0.016	1.431LysPro: 1.431 ± 0.026	0.707LysGln: 0.707 ± 0.02	1.578LysArg: 1.578 ± 0.031	1.39LysSer: 1.39 ± 0.028	1.455LysThr: 1.455 ± 0.037	1.933LysVal: 1.933 ± 0.035	0.345LysTrp: 0.345 ± 0.013	0.484LysTyr: 0.484 ± 0.018	0.0LysXaa: 0.0 ± 0.0
Leu	13.877LeuAla: 13.877 ± 0.11	0.77LeuCys: 0.77 ± 0.022	6.659LeuAsp: 6.659 ± 0.067	4.474LeuGlu: 4.474 ± 0.05	2.8LeuPhe: 2.8 ± 0.049	8.907LeuGly: 8.907 ± 0.071	1.986LeuHis: 1.986 ± 0.032	3.95LeuIle: 3.95 ± 0.047	1.899LeuLys: 1.899 ± 0.036	9.616LeuLeu: 9.616 ± 0.084	1.76LeuMet: 1.76 ± 0.034	2.138LeuAsn: 2.138 ± 0.036	5.628LeuPro: 5.628 ± 0.057	2.495LeuGln: 2.495 ± 0.036	7.209LeuArg: 7.209 ± 0.068	5.627LeuSer: 5.627 ± 0.056	6.519LeuThr: 6.519 ± 0.064	9.138LeuVal: 9.138 ± 0.081	1.147LeuTrp: 1.147 ± 0.026	1.632LeuTyr: 1.632 ± 0.028	0.002LeuXaa: 0.002 ± 0.001
Met	2.65MetAla: 2.65 ± 0.037	0.16MetCys: 0.16 ± 0.009	0.913MetAsp: 0.913 ± 0.02	0.719MetGlu: 0.719 ± 0.02	0.654MetPhe: 0.654 ± 0.02	1.54MetGly: 1.54 ± 0.031	0.396MetHis: 0.396 ± 0.015	0.883MetIle: 0.883 ± 0.022	0.495MetLys: 0.495 ± 0.016	2.015MetLeu: 2.015 ± 0.032	0.448MetMet: 0.448 ± 0.018	0.499MetAsn: 0.499 ± 0.016	1.223MetPro: 1.223 ± 0.024	0.542MetGln: 0.542 ± 0.02	1.514MetArg: 1.514 ± 0.025	1.616MetSer: 1.616 ± 0.03	1.973MetThr: 1.973 ± 0.032	1.731MetVal: 1.731 ± 0.033	0.257MetTrp: 0.257 ± 0.013	0.351MetTyr: 0.351 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	2.744AsnAla: 2.744 ± 0.041	0.174AsnCys: 0.174 ± 0.009	1.3AsnAsp: 1.3 ± 0.027	0.953AsnGlu: 0.953 ± 0.021	0.712AsnPhe: 0.712 ± 0.017	2.222AsnGly: 2.222 ± 0.047	0.472AsnHis: 0.472 ± 0.017	0.804AsnIle: 0.804 ± 0.022	0.42AsnLys: 0.42 ± 0.017	2.173AsnLeu: 2.173 ± 0.035	0.43AsnMet: 0.43 ± 0.014	0.519AsnAsn: 0.519 ± 0.022	1.757AsnPro: 1.757 ± 0.034	0.657AsnGln: 0.657 ± 0.018	1.46AsnArg: 1.46 ± 0.029	1.117AsnSer: 1.117 ± 0.024	1.308AsnThr: 1.308 ± 0.032	2.046AsnVal: 2.046 ± 0.032	0.374AsnTrp: 0.374 ± 0.013	0.583AsnTyr: 0.583 ± 0.019	0.0AsnXaa: 0.0 ± 0.0
Pro	6.857ProAla: 6.857 ± 0.07	0.278ProCys: 0.278 ± 0.013	4.17ProAsp: 4.17 ± 0.054	3.567ProGlu: 3.567 ± 0.039	1.639ProPhe: 1.639 ± 0.03	5.324ProGly: 5.324 ± 0.065	1.202ProHis: 1.202 ± 0.025	2.035ProIle: 2.035 ± 0.03	1.325ProLys: 1.325 ± 0.028	5.017ProLeu: 5.017 ± 0.061	1.233ProMet: 1.233 ± 0.025	1.337ProAsn: 1.337 ± 0.029	3.508ProPro: 3.508 ± 0.08	1.604ProGln: 1.604 ± 0.029	3.269ProArg: 3.269 ± 0.038	3.243ProSer: 3.243 ± 0.041	3.751ProThr: 3.751 ± 0.05	5.073ProVal: 5.073 ± 0.051	0.882ProTrp: 0.882 ± 0.025	1.16ProTyr: 1.16 ± 0.025	0.001ProXaa: 0.001 ± 0.0
Gln	3.351GlnAla: 3.351 ± 0.042	0.211GlnCys: 0.211 ± 0.011	1.223GlnAsp: 1.223 ± 0.024	1.081GlnGlu: 1.081 ± 0.026	0.979GlnPhe: 0.979 ± 0.021	1.92GlnGly: 1.92 ± 0.036	0.665GlnHis: 0.665 ± 0.021	1.397GlnIle: 1.397 ± 0.027	0.645GlnLys: 0.645 ± 0.02	3.339GlnLeu: 3.339 ± 0.049	0.651GlnMet: 0.651 ± 0.02	0.64GlnAsn: 0.64 ± 0.017	1.747GlnPro: 1.747 ± 0.036	1.15GlnGln: 1.15 ± 0.031	2.531GlnArg: 2.531 ± 0.04	1.523GlnSer: 1.523 ± 0.028	1.561GlnThr: 1.561 ± 0.03	2.339GlnVal: 2.339 ± 0.032	0.55GlnTrp: 0.55 ± 0.017	0.658GlnTyr: 0.658 ± 0.019	0.0GlnXaa: 0.0 ± 0.0
Arg	7.739ArgAla: 7.739 ± 0.076	0.495ArgCys: 0.495 ± 0.017	4.282ArgAsp: 4.282 ± 0.044	3.657ArgGlu: 3.657 ± 0.051	2.324ArgPhe: 2.324 ± 0.036	5.068ArgGly: 5.068 ± 0.054	1.679ArgHis: 1.679 ± 0.028	3.25ArgIle: 3.25 ± 0.038	1.52ArgLys: 1.52 ± 0.031	7.073ArgLeu: 7.073 ± 0.065	1.689ArgMet: 1.689 ± 0.029	1.597ArgAsn: 1.597 ± 0.03	3.711ArgPro: 3.711 ± 0.049	2.144ArgGln: 2.144 ± 0.036	6.129ArgArg: 6.129 ± 0.072	3.928ArgSer: 3.928 ± 0.049	4.175ArgThr: 4.175 ± 0.05	5.652ArgVal: 5.652 ± 0.054	1.256ArgTrp: 1.256 ± 0.03	1.692ArgTyr: 1.692 ± 0.027	0.001ArgXaa: 0.001 ± 0.001
Ser	7.17SerAla: 7.17 ± 0.075	0.399SerCys: 0.399 ± 0.016	3.363SerAsp: 3.363 ± 0.042	2.592SerGlu: 2.592 ± 0.036	1.76SerPhe: 1.76 ± 0.031	5.6SerGly: 5.6 ± 0.054	1.113SerHis: 1.113 ± 0.023	2.238SerIle: 2.238 ± 0.03	1.233SerLys: 1.233 ± 0.027	4.938SerLeu: 4.938 ± 0.05	1.369SerMet: 1.369 ± 0.028	1.165SerAsn: 1.165 ± 0.024	3.161SerPro: 3.161 ± 0.048	1.504SerGln: 1.504 ± 0.029	3.645SerArg: 3.645 ± 0.041	3.464SerSer: 3.464 ± 0.048	3.748SerThr: 3.748 ± 0.05	4.926SerVal: 4.926 ± 0.05	0.913SerTrp: 0.913 ± 0.02	1.173SerTyr: 1.173 ± 0.022	0.0SerXaa: 0.0 ± 0.0
Thr	8.095ThrAla: 8.095 ± 0.074	0.428ThrCys: 0.428 ± 0.017	3.887ThrAsp: 3.887 ± 0.048	3.099ThrGlu: 3.099 ± 0.04	2.007ThrPhe: 2.007 ± 0.034	5.928ThrGly: 5.928 ± 0.061	1.207ThrHis: 1.207 ± 0.025	2.499ThrIle: 2.499 ± 0.042	1.419ThrLys: 1.419 ± 0.032	5.781ThrLeu: 5.781 ± 0.053	1.327ThrMet: 1.327 ± 0.029	1.377ThrAsn: 1.377 ± 0.034	4.074ThrPro: 4.074 ± 0.053	1.453ThrGln: 1.453 ± 0.031	3.643ThrArg: 3.643 ± 0.04	3.62ThrSer: 3.62 ± 0.047	4.346ThrThr: 4.346 ± 0.066	6.268ThrVal: 6.268 ± 0.062	0.905ThrTrp: 0.905 ± 0.02	1.346ThrTyr: 1.346 ± 0.025	0.001ThrXaa: 0.001 ± 0.0
Val	12.499ValAla: 12.499 ± 0.087	0.757ValCys: 0.757 ± 0.021	7.232ValAsp: 7.232 ± 0.061	5.024ValGlu: 5.024 ± 0.063	2.73ValPhe: 2.73 ± 0.037	7.836ValGly: 7.836 ± 0.071	1.877ValHis: 1.877 ± 0.031	3.844ValIle: 3.844 ± 0.045	1.844ValLys: 1.844 ± 0.035	9.094ValLeu: 9.094 ± 0.082	1.718ValMet: 1.718 ± 0.029	2.264ValAsn: 2.264 ± 0.04	4.777ValPro: 4.777 ± 0.051	2.138ValGln: 2.138 ± 0.032	5.757ValArg: 5.757 ± 0.056	4.912ValSer: 4.912 ± 0.054	6.203ValThr: 6.203 ± 0.069	9.945ValVal: 9.945 ± 0.093	1.064ValTrp: 1.064 ± 0.024	1.692ValTyr: 1.692 ± 0.032	0.0ValXaa: 0.0 ± 0.0
Trp	1.52TrpAla: 1.52 ± 0.03	0.148TrpCys: 0.148 ± 0.009	0.812TrpAsp: 0.812 ± 0.022	0.587TrpGlu: 0.587 ± 0.017	0.527TrpPhe: 0.527 ± 0.016	1.076TrpGly: 1.076 ± 0.024	0.365TrpHis: 0.365 ± 0.013	0.638TrpIle: 0.638 ± 0.02	0.329TrpLys: 0.329 ± 0.013	1.689TrpLeu: 1.689 ± 0.038	0.335TrpMet: 0.335 ± 0.013	0.442TrpAsn: 0.442 ± 0.017	0.829TrpPro: 0.829 ± 0.023	0.61TrpGln: 0.61 ± 0.017	1.168TrpArg: 1.168 ± 0.024	0.949TrpSer: 0.949 ± 0.024	0.938TrpThr: 0.938 ± 0.022	1.142TrpVal: 1.142 ± 0.027	0.341TrpTrp: 0.341 ± 0.014	0.32TrpTyr: 0.32 ± 0.012	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.446TyrAla: 2.446 ± 0.036	0.219TyrCys: 0.219 ± 0.011	1.51TyrAsp: 1.51 ± 0.031	1.042TyrGlu: 1.042 ± 0.023	0.79TyrPhe: 0.79 ± 0.019	2.101TyrGly: 2.101 ± 0.035	0.41TyrHis: 0.41 ± 0.013	0.541TyrIle: 0.541 ± 0.015	0.372TyrLys: 0.372 ± 0.015	2.287TyrLeu: 2.287 ± 0.036	0.274TyrMet: 0.274 ± 0.011	0.427TyrAsn: 0.427 ± 0.016	1.157TyrPro: 1.157 ± 0.021	0.662TyrGln: 0.662 ± 0.018	1.701TyrArg: 1.701 ± 0.031	1.125TyrSer: 1.125 ± 0.026	1.181TyrThr: 1.181 ± 0.027	1.903TyrVal: 1.903 ± 0.035	0.332TyrTrp: 0.332 ± 0.014	0.505TyrTyr: 0.505 ± 0.02	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.001	0.001XaaCys: 0.001 ± 0.0	0.001XaaAsp: 0.001 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.001XaaPhe: 0.001 ± 0.001	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.001XaaAsn: 0.001 ± 0.001	0.001XaaPro: 0.001 ± 0.001	0.001XaaGln: 0.001 ± 0.001	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.001XaaThr: 0.001 ± 0.0	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.001XaaXaa: 0.001 ± 0.001

Statistics based on 5995 proteins (1952207 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---