Postia placenta MAD-698-R-SB12
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12517 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1X6N8Q1|A0A1X6N8Q1_9APHY Uncharacterized protein (Fragment) OS=Postia placenta MAD-698-R-SB12 OX=670580 GN=POSPLADRAFT_1135962 PE=3 SV=1
MM1 pKa = 7.29 TSVSTTEE8 pKa = 3.9 PQSLFRR14 pKa = 11.84 MARR17 pKa = 11.84 SKK19 pKa = 10.63 LQSAIGSGVKK29 pKa = 8.71 DD30 pKa = 3.28 TCSLYY35 pKa = 10.64 RR36 pKa = 11.84 WVLLKK41 pKa = 11.12 NSMTHH46 pKa = 5.27 STPSDD51 pKa = 3.09 AAAASSDD58 pKa = 3.76 KK59 pKa = 11.5 ADD61 pKa = 3.52 VTCVYY66 pKa = 10.48 RR67 pKa = 11.84 PDD69 pKa = 3.38 VDD71 pKa = 3.31 QGRR74 pKa = 11.84 EE75 pKa = 3.97 EE76 pKa = 4.62 DD77 pKa = 4.84 DD78 pKa = 3.5 EE79 pKa = 4.75 DD80 pKa = 6.02 AFMFPDD86 pKa = 4.43 PGEE89 pKa = 4.36 ADD91 pKa = 3.14 AANPEE96 pKa = 4.28 QAWVNSLLEE105 pKa = 4.14 SLGDD109 pKa = 3.64 EE110 pKa = 4.94 DD111 pKa = 6.26 DD112 pKa = 4.98 DD113 pKa = 4.29 LVEE116 pKa = 4.52 EE117 pKa = 4.81 GTVSVTACPVDD128 pKa = 5.49 DD129 pKa = 5.59 DD130 pKa = 5.84 DD131 pKa = 5.85 EE132 pKa = 4.81 PLSPLCSPMSSSDD145 pKa = 3.52 DD146 pKa = 3.76 LVSHH150 pKa = 6.73 SSYY153 pKa = 10.54 YY154 pKa = 10.43 CNPHH158 pKa = 7.44 AIPFPYY164 pKa = 9.75 PIPYY168 pKa = 9.33 PPLRR172 pKa = 11.84 DD173 pKa = 3.67 SPLSPWLEE181 pKa = 4.35 GEE183 pKa = 4.15 SSRR186 pKa = 11.84 DD187 pKa = 3.34 SLIDD191 pKa = 3.38 ARR193 pKa = 11.84 PPLYY197 pKa = 10.24 HH198 pKa = 7.73 DD199 pKa = 4.21 PLPYY203 pKa = 10.93 YY204 pKa = 10.68 NVDD207 pKa = 3.51 DD208 pKa = 4.57 TEE210 pKa = 4.64 DD211 pKa = 4.16 LPVPDD216 pKa = 5.83 AIEE219 pKa = 4.42 DD220 pKa = 3.94 TSDD223 pKa = 3.91 DD224 pKa = 4.19 EE225 pKa = 5.97 SDD227 pKa = 3.79 APCTPFEE234 pKa = 4.13 QSTSSLSPVDD244 pKa = 3.44 PAFIPLPPEE253 pKa = 3.87 RR254 pKa = 11.84 RR255 pKa = 11.84 VHH257 pKa = 6.1 SLQPQVYY264 pKa = 8.94 IDD266 pKa = 3.69 TDD268 pKa = 3.27 DD269 pKa = 3.84 SYY271 pKa = 11.73 FYY273 pKa = 10.36 PFEE276 pKa = 5.14 LDD278 pKa = 3.31 PVPPHH283 pKa = 7.13 DD284 pKa = 5.15 ANAVDD289 pKa = 4.25 PARR292 pKa = 11.84 AFHH295 pKa = 6.34 PSVYY299 pKa = 9.82 QEE301 pKa = 4.27 CC302 pKa = 3.79
Molecular weight: 33.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.717
IPC2_protein 3.757
IPC_protein 3.795
Toseland 3.567
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.732
Rodwell 3.617
Grimsley 3.465
Solomon 3.783
Lehninger 3.745
Nozaki 3.897
DTASelect 4.164
Thurlkill 3.617
EMBOSS 3.745
Sillero 3.91
Patrickios 0.757
IPC_peptide 3.783
IPC2_peptide 3.884
IPC2.peptide.svr19 3.801
Protein with the highest isoelectric point:
>tr|A0A1X6MJX4|A0A1X6MJX4_9APHY Uncharacterized protein OS=Postia placenta MAD-698-R-SB12 OX=670580 GN=POSPLADRAFT_1160356 PE=4 SV=1
MM1 pKa = 7.67 NEE3 pKa = 3.75 FSTDD7 pKa = 3.31 NTSKK11 pKa = 11.04 RR12 pKa = 11.84 LDD14 pKa = 3.31 TGRR17 pKa = 11.84 LCNFGAEE24 pKa = 4.39 SGWRR28 pKa = 11.84 EE29 pKa = 3.88 RR30 pKa = 11.84 CSQSQTTKK38 pKa = 10.79 LLFGARR44 pKa = 11.84 SGVTGIGRR52 pKa = 11.84 RR53 pKa = 11.84 WPVADD58 pKa = 3.44 CWKK61 pKa = 10.38 SVSDD65 pKa = 3.48 SRR67 pKa = 11.84 SRR69 pKa = 11.84 SDD71 pKa = 3.71 AVSLTLVYY79 pKa = 8.62 TLCCAARR86 pKa = 11.84 SRR88 pKa = 11.84 RR89 pKa = 11.84 YY90 pKa = 9.33 EE91 pKa = 3.61 RR92 pKa = 11.84 AGQGRR97 pKa = 11.84 VGSAGSFKK105 pKa = 10.61 GVRR108 pKa = 11.84 CRR110 pKa = 4.29
Molecular weight: 12.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.353
IPC2_protein 9.355
IPC_protein 10.028
Toseland 10.409
ProMoST 10.423
Dawson 10.526
Bjellqvist 10.292
Wikipedia 10.73
Rodwell 10.643
Grimsley 10.57
Solomon 10.643
Lehninger 10.613
Nozaki 10.511
DTASelect 10.248
Thurlkill 10.438
EMBOSS 10.818
Sillero 10.496
Patrickios 10.423
IPC_peptide 10.643
IPC2_peptide 9.809
IPC2.peptide.svr19 8.553
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12515
2
12517
5369358
49
4280
429.0
47.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.377 ± 0.019
1.371 ± 0.009
5.661 ± 0.015
5.914 ± 0.02
3.475 ± 0.013
6.499 ± 0.019
2.612 ± 0.009
4.62 ± 0.016
4.082 ± 0.017
9.241 ± 0.025
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.123 ± 0.008
3.066 ± 0.01
6.517 ± 0.025
3.701 ± 0.012
6.751 ± 0.023
8.275 ± 0.023
5.964 ± 0.014
6.6 ± 0.017
1.478 ± 0.008
2.672 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here