Amino acid dipepetide frequency for Streptomyces phage Fabian

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.23AlaAla: 11.23 ± 1.146
0.96AlaCys: 0.96 ± 0.296
4.802AlaAsp: 4.802 ± 0.684
6.649AlaGlu: 6.649 ± 0.627
2.216AlaPhe: 2.216 ± 0.343
8.718AlaGly: 8.718 ± 0.887
1.33AlaHis: 1.33 ± 0.286
3.694AlaIle: 3.694 ± 0.649
6.723AlaLys: 6.723 ± 0.846
7.462AlaLeu: 7.462 ± 0.757
2.29AlaMet: 2.29 ± 0.41
4.211AlaAsn: 4.211 ± 0.682
3.472AlaPro: 3.472 ± 0.791
4.95AlaGln: 4.95 ± 0.695
5.098AlaArg: 5.098 ± 0.713
6.354AlaSer: 6.354 ± 0.668
6.871AlaThr: 6.871 ± 0.891
7.684AlaVal: 7.684 ± 0.873
1.995AlaTrp: 1.995 ± 0.406
3.62AlaTyr: 3.62 ± 0.699
0.0AlaXaa: 0.0 ± 0.0
Cys
0.443CysAla: 0.443 ± 0.196
0.074CysCys: 0.074 ± 0.077
0.517CysAsp: 0.517 ± 0.226
0.443CysGlu: 0.443 ± 0.181
0.074CysPhe: 0.074 ± 0.071
0.517CysGly: 0.517 ± 0.203
0.222CysHis: 0.222 ± 0.124
0.148CysIle: 0.148 ± 0.109
0.665CysLys: 0.665 ± 0.295
0.591CysLeu: 0.591 ± 0.241
0.222CysMet: 0.222 ± 0.137
0.074CysAsn: 0.074 ± 0.077
1.108CysPro: 1.108 ± 0.396
0.369CysGln: 0.369 ± 0.207
0.296CysArg: 0.296 ± 0.162
0.517CysSer: 0.517 ± 0.212
0.517CysThr: 0.517 ± 0.3
0.96CysVal: 0.96 ± 0.32
0.074CysTrp: 0.074 ± 0.073
0.074CysTyr: 0.074 ± 0.086
0.0CysXaa: 0.0 ± 0.0
Asp
6.354AspAla: 6.354 ± 0.688
0.443AspCys: 0.443 ± 0.203
4.285AspAsp: 4.285 ± 0.581
4.137AspGlu: 4.137 ± 0.594
1.995AspPhe: 1.995 ± 0.367
5.024AspGly: 5.024 ± 0.605
1.404AspHis: 1.404 ± 0.367
3.472AspIle: 3.472 ± 0.494
2.881AspLys: 2.881 ± 0.389
4.285AspLeu: 4.285 ± 0.576
2.66AspMet: 2.66 ± 0.637
2.29AspAsn: 2.29 ± 0.502
4.507AspPro: 4.507 ± 0.593
2.29AspGln: 2.29 ± 0.425
3.177AspArg: 3.177 ± 0.434
3.472AspSer: 3.472 ± 0.653
3.62AspThr: 3.62 ± 0.493
4.876AspVal: 4.876 ± 0.701
1.33AspTrp: 1.33 ± 0.315
2.881AspTyr: 2.881 ± 0.406
0.0AspXaa: 0.0 ± 0.0
Glu
5.763GluAla: 5.763 ± 0.691
0.443GluCys: 0.443 ± 0.234
3.251GluAsp: 3.251 ± 0.457
3.694GluGlu: 3.694 ± 0.534
1.847GluPhe: 1.847 ± 0.38
4.581GluGly: 4.581 ± 0.497
1.108GluHis: 1.108 ± 0.328
2.955GluIle: 2.955 ± 0.461
3.029GluLys: 3.029 ± 0.591
5.467GluLeu: 5.467 ± 0.542
0.96GluMet: 0.96 ± 0.303
2.364GluAsn: 2.364 ± 0.357
1.552GluPro: 1.552 ± 0.421
2.734GluGln: 2.734 ± 0.377
2.955GluArg: 2.955 ± 0.499
2.586GluSer: 2.586 ± 0.395
3.029GluThr: 3.029 ± 0.445
3.768GluVal: 3.768 ± 0.621
1.108GluTrp: 1.108 ± 0.24
2.586GluTyr: 2.586 ± 0.525
0.0GluXaa: 0.0 ± 0.0
Phe
2.364PheAla: 2.364 ± 0.481
0.369PheCys: 0.369 ± 0.179
2.586PheAsp: 2.586 ± 0.454
1.995PheGlu: 1.995 ± 0.412
0.887PhePhe: 0.887 ± 0.236
3.325PheGly: 3.325 ± 0.616
0.517PheHis: 0.517 ± 0.18
1.256PheIle: 1.256 ± 0.349
1.182PheLys: 1.182 ± 0.263
1.995PheLeu: 1.995 ± 0.418
0.887PheMet: 0.887 ± 0.276
1.478PheAsn: 1.478 ± 0.366
1.182PhePro: 1.182 ± 0.389
1.182PheGln: 1.182 ± 0.34
1.699PheArg: 1.699 ± 0.42
2.438PheSer: 2.438 ± 0.382
2.881PheThr: 2.881 ± 0.466
1.478PheVal: 1.478 ± 0.303
0.369PheTrp: 0.369 ± 0.174
1.182PheTyr: 1.182 ± 0.358
0.0PheXaa: 0.0 ± 0.0
Gly
8.275GlyAla: 8.275 ± 0.907
0.665GlyCys: 0.665 ± 0.228
3.916GlyAsp: 3.916 ± 0.549
5.172GlyGlu: 5.172 ± 0.614
2.955GlyPhe: 2.955 ± 0.539
8.792GlyGly: 8.792 ± 1.104
1.625GlyHis: 1.625 ± 0.393
3.842GlyIle: 3.842 ± 0.7
5.984GlyLys: 5.984 ± 0.784
6.576GlyLeu: 6.576 ± 0.812
2.069GlyMet: 2.069 ± 0.34
3.029GlyAsn: 3.029 ± 0.508
3.103GlyPro: 3.103 ± 0.453
3.916GlyGln: 3.916 ± 0.648
3.177GlyArg: 3.177 ± 0.478
5.098GlySer: 5.098 ± 0.554
6.206GlyThr: 6.206 ± 1.005
6.132GlyVal: 6.132 ± 0.753
1.478GlyTrp: 1.478 ± 0.293
3.177GlyTyr: 3.177 ± 0.615
0.0GlyXaa: 0.0 ± 0.0
His
1.33HisAla: 1.33 ± 0.258
0.148HisCys: 0.148 ± 0.101
1.552HisAsp: 1.552 ± 0.414
0.739HisGlu: 0.739 ± 0.279
0.591HisPhe: 0.591 ± 0.179
1.256HisGly: 1.256 ± 0.274
0.369HisHis: 0.369 ± 0.166
0.665HisIle: 0.665 ± 0.253
1.034HisLys: 1.034 ± 0.267
1.182HisLeu: 1.182 ± 0.288
0.369HisMet: 0.369 ± 0.164
0.517HisAsn: 0.517 ± 0.196
0.739HisPro: 0.739 ± 0.25
0.591HisGln: 0.591 ± 0.206
0.296HisArg: 0.296 ± 0.158
1.182HisSer: 1.182 ± 0.24
0.517HisThr: 0.517 ± 0.192
1.625HisVal: 1.625 ± 0.291
0.222HisTrp: 0.222 ± 0.167
0.887HisTyr: 0.887 ± 0.263
0.0HisXaa: 0.0 ± 0.0
Ile
4.728IleAla: 4.728 ± 0.589
0.369IleCys: 0.369 ± 0.21
3.546IleAsp: 3.546 ± 0.529
1.995IleGlu: 1.995 ± 0.345
1.108IlePhe: 1.108 ± 0.29
3.029IleGly: 3.029 ± 0.571
0.96IleHis: 0.96 ± 0.241
2.069IleIle: 2.069 ± 0.355
2.734IleLys: 2.734 ± 0.516
3.103IleLeu: 3.103 ± 0.43
0.96IleMet: 0.96 ± 0.231
1.404IleAsn: 1.404 ± 0.364
1.625IlePro: 1.625 ± 0.337
1.108IleGln: 1.108 ± 0.292
2.808IleArg: 2.808 ± 0.499
2.734IleSer: 2.734 ± 0.49
3.694IleThr: 3.694 ± 0.518
2.143IleVal: 2.143 ± 0.414
0.443IleTrp: 0.443 ± 0.163
1.33IleTyr: 1.33 ± 0.305
0.0IleXaa: 0.0 ± 0.0
Lys
7.019LysAla: 7.019 ± 0.9
0.443LysCys: 0.443 ± 0.204
3.842LysAsp: 3.842 ± 0.563
2.216LysGlu: 2.216 ± 0.359
2.66LysPhe: 2.66 ± 0.499
4.137LysGly: 4.137 ± 0.655
0.96LysHis: 0.96 ± 0.268
2.216LysIle: 2.216 ± 0.394
3.325LysLys: 3.325 ± 0.624
4.433LysLeu: 4.433 ± 0.626
1.699LysMet: 1.699 ± 0.413
2.143LysAsn: 2.143 ± 0.622
2.66LysPro: 2.66 ± 0.643
2.808LysGln: 2.808 ± 0.45
2.586LysArg: 2.586 ± 0.552
2.438LysSer: 2.438 ± 0.488
3.251LysThr: 3.251 ± 0.485
4.95LysVal: 4.95 ± 0.718
1.182LysTrp: 1.182 ± 0.312
2.29LysTyr: 2.29 ± 0.455
0.0LysXaa: 0.0 ± 0.0
Leu
6.28LeuAla: 6.28 ± 0.92
0.369LeuCys: 0.369 ± 0.16
5.024LeuAsp: 5.024 ± 0.703
3.472LeuGlu: 3.472 ± 0.394
3.103LeuPhe: 3.103 ± 0.565
5.393LeuGly: 5.393 ± 0.64
0.591LeuHis: 0.591 ± 0.203
2.808LeuIle: 2.808 ± 0.475
4.581LeuLys: 4.581 ± 0.663
5.32LeuLeu: 5.32 ± 0.612
1.847LeuMet: 1.847 ± 0.371
2.66LeuAsn: 2.66 ± 0.37
3.546LeuPro: 3.546 ± 0.509
3.546LeuGln: 3.546 ± 0.556
5.024LeuArg: 5.024 ± 0.714
5.024LeuSer: 5.024 ± 0.682
6.058LeuThr: 6.058 ± 0.533
5.763LeuVal: 5.763 ± 0.604
0.887LeuTrp: 0.887 ± 0.339
2.586LeuTyr: 2.586 ± 0.419
0.0LeuXaa: 0.0 ± 0.0
Met
4.581MetAla: 4.581 ± 0.512
0.074MetCys: 0.074 ± 0.077
1.995MetAsp: 1.995 ± 0.298
1.182MetGlu: 1.182 ± 0.303
0.517MetPhe: 0.517 ± 0.19
2.66MetGly: 2.66 ± 0.421
0.074MetHis: 0.074 ± 0.083
1.108MetIle: 1.108 ± 0.337
1.256MetLys: 1.256 ± 0.319
1.33MetLeu: 1.33 ± 0.334
0.813MetMet: 0.813 ± 0.21
1.256MetAsn: 1.256 ± 0.265
1.108MetPro: 1.108 ± 0.332
1.33MetGln: 1.33 ± 0.349
1.478MetArg: 1.478 ± 0.3
2.734MetSer: 2.734 ± 0.481
1.404MetThr: 1.404 ± 0.312
1.552MetVal: 1.552 ± 0.26
0.443MetTrp: 0.443 ± 0.162
0.369MetTyr: 0.369 ± 0.212
0.0MetXaa: 0.0 ± 0.0
Asn
3.99AsnAla: 3.99 ± 0.55
0.443AsnCys: 0.443 ± 0.184
2.364AsnAsp: 2.364 ± 0.422
2.66AsnGlu: 2.66 ± 0.455
1.33AsnPhe: 1.33 ± 0.297
4.064AsnGly: 4.064 ± 0.668
0.813AsnHis: 0.813 ± 0.2
1.404AsnIle: 1.404 ± 0.29
2.29AsnLys: 2.29 ± 0.449
3.916AsnLeu: 3.916 ± 0.635
0.443AsnMet: 0.443 ± 0.184
1.847AsnAsn: 1.847 ± 0.44
2.881AsnPro: 2.881 ± 0.539
1.625AsnGln: 1.625 ± 0.369
1.995AsnArg: 1.995 ± 0.46
2.438AsnSer: 2.438 ± 0.442
2.143AsnThr: 2.143 ± 0.297
2.143AsnVal: 2.143 ± 0.468
1.182AsnTrp: 1.182 ± 0.293
1.33AsnTyr: 1.33 ± 0.329
0.0AsnXaa: 0.0 ± 0.0
Pro
4.876ProAla: 4.876 ± 0.86
0.443ProCys: 0.443 ± 0.2
2.586ProAsp: 2.586 ± 0.456
2.512ProGlu: 2.512 ± 0.341
1.404ProPhe: 1.404 ± 0.312
4.285ProGly: 4.285 ± 0.68
0.739ProHis: 0.739 ± 0.218
0.96ProIle: 0.96 ± 0.245
1.995ProLys: 1.995 ± 0.477
3.029ProLeu: 3.029 ± 0.467
1.108ProMet: 1.108 ± 0.341
1.921ProAsn: 1.921 ± 0.303
1.921ProPro: 1.921 ± 0.437
1.699ProGln: 1.699 ± 0.352
2.66ProArg: 2.66 ± 0.435
2.955ProSer: 2.955 ± 0.488
3.62ProThr: 3.62 ± 0.626
3.029ProVal: 3.029 ± 0.475
0.887ProTrp: 0.887 ± 0.209
1.552ProTyr: 1.552 ± 0.393
0.0ProXaa: 0.0 ± 0.0
Gln
5.984GlnAla: 5.984 ± 0.75
0.222GlnCys: 0.222 ± 0.159
3.325GlnAsp: 3.325 ± 0.425
2.29GlnGlu: 2.29 ± 0.487
0.813GlnPhe: 0.813 ± 0.294
2.364GlnGly: 2.364 ± 0.506
0.222GlnHis: 0.222 ± 0.132
2.069GlnIle: 2.069 ± 0.456
2.586GlnLys: 2.586 ± 0.5
3.842GlnLeu: 3.842 ± 0.495
1.552GlnMet: 1.552 ± 0.274
1.108GlnAsn: 1.108 ± 0.212
1.404GlnPro: 1.404 ± 0.405
2.438GlnGln: 2.438 ± 0.482
2.808GlnArg: 2.808 ± 0.45
3.029GlnSer: 3.029 ± 0.492
3.029GlnThr: 3.029 ± 0.479
3.103GlnVal: 3.103 ± 0.477
0.296GlnTrp: 0.296 ± 0.138
1.625GlnTyr: 1.625 ± 0.351
0.0GlnXaa: 0.0 ± 0.0
Arg
4.581ArgAla: 4.581 ± 0.646
0.517ArgCys: 0.517 ± 0.237
3.916ArgAsp: 3.916 ± 0.521
2.955ArgGlu: 2.955 ± 0.463
1.995ArgPhe: 1.995 ± 0.399
3.916ArgGly: 3.916 ± 0.467
0.813ArgHis: 0.813 ± 0.213
2.143ArgIle: 2.143 ± 0.359
2.512ArgLys: 2.512 ± 0.485
3.029ArgLeu: 3.029 ± 0.501
1.404ArgMet: 1.404 ± 0.32
2.512ArgAsn: 2.512 ± 0.515
2.069ArgPro: 2.069 ± 0.384
1.995ArgGln: 1.995 ± 0.43
2.29ArgArg: 2.29 ± 0.705
2.808ArgSer: 2.808 ± 0.431
3.472ArgThr: 3.472 ± 0.443
4.064ArgVal: 4.064 ± 0.609
1.182ArgTrp: 1.182 ± 0.35
1.847ArgTyr: 1.847 ± 0.358
0.0ArgXaa: 0.0 ± 0.0
Ser
5.246SerAla: 5.246 ± 0.676
0.591SerCys: 0.591 ± 0.261
4.064SerAsp: 4.064 ± 0.634
2.808SerGlu: 2.808 ± 0.422
2.364SerPhe: 2.364 ± 0.383
7.832SerGly: 7.832 ± 0.864
0.887SerHis: 0.887 ± 0.221
2.955SerIle: 2.955 ± 0.392
1.995SerLys: 1.995 ± 0.373
4.211SerLeu: 4.211 ± 0.519
1.773SerMet: 1.773 ± 0.359
3.325SerAsn: 3.325 ± 0.527
2.808SerPro: 2.808 ± 0.389
2.586SerGln: 2.586 ± 0.414
2.734SerArg: 2.734 ± 0.477
4.876SerSer: 4.876 ± 0.867
4.359SerThr: 4.359 ± 0.706
4.137SerVal: 4.137 ± 0.607
1.182SerTrp: 1.182 ± 0.336
2.438SerTyr: 2.438 ± 0.429
0.0SerXaa: 0.0 ± 0.0
Thr
6.28ThrAla: 6.28 ± 1.008
0.369ThrCys: 0.369 ± 0.183
4.581ThrAsp: 4.581 ± 0.543
2.808ThrGlu: 2.808 ± 0.487
2.29ThrPhe: 2.29 ± 0.314
6.354ThrGly: 6.354 ± 0.756
1.256ThrHis: 1.256 ± 0.34
3.103ThrIle: 3.103 ± 0.506
3.842ThrLys: 3.842 ± 0.625
5.541ThrLeu: 5.541 ± 0.751
1.847ThrMet: 1.847 ± 0.381
3.103ThrAsn: 3.103 ± 0.577
3.99ThrPro: 3.99 ± 0.568
2.734ThrGln: 2.734 ± 0.506
2.66ThrArg: 2.66 ± 0.45
4.581ThrSer: 4.581 ± 0.684
5.393ThrThr: 5.393 ± 0.561
4.581ThrVal: 4.581 ± 0.603
1.034ThrTrp: 1.034 ± 0.188
3.103ThrTyr: 3.103 ± 0.405
0.0ThrXaa: 0.0 ± 0.0
Val
6.206ValAla: 6.206 ± 0.822
0.517ValCys: 0.517 ± 0.251
5.024ValAsp: 5.024 ± 0.696
4.433ValGlu: 4.433 ± 0.997
1.995ValPhe: 1.995 ± 0.344
4.655ValGly: 4.655 ± 0.562
1.478ValHis: 1.478 ± 0.347
2.955ValIle: 2.955 ± 0.409
4.802ValLys: 4.802 ± 0.655
4.802ValLeu: 4.802 ± 0.504
2.069ValMet: 2.069 ± 0.303
3.399ValAsn: 3.399 ± 0.669
2.586ValPro: 2.586 ± 0.532
3.251ValGln: 3.251 ± 0.485
3.916ValArg: 3.916 ± 0.533
4.655ValSer: 4.655 ± 0.584
5.763ValThr: 5.763 ± 0.766
3.546ValVal: 3.546 ± 0.77
1.108ValTrp: 1.108 ± 0.286
3.029ValTyr: 3.029 ± 0.478
0.0ValXaa: 0.0 ± 0.0
Trp
1.625TrpAla: 1.625 ± 0.308
0.074TrpCys: 0.074 ± 0.065
1.33TrpAsp: 1.33 ± 0.342
1.108TrpGlu: 1.108 ± 0.306
0.517TrpPhe: 0.517 ± 0.196
0.813TrpGly: 0.813 ± 0.253
0.148TrpHis: 0.148 ± 0.105
0.739TrpIle: 0.739 ± 0.247
1.108TrpLys: 1.108 ± 0.265
1.034TrpLeu: 1.034 ± 0.275
0.443TrpMet: 0.443 ± 0.175
0.739TrpAsn: 0.739 ± 0.223
0.665TrpPro: 0.665 ± 0.244
1.034TrpGln: 1.034 ± 0.245
0.739TrpArg: 0.739 ± 0.205
0.887TrpSer: 0.887 ± 0.302
1.404TrpThr: 1.404 ± 0.293
1.773TrpVal: 1.773 ± 0.294
0.443TrpTrp: 0.443 ± 0.161
0.665TrpTyr: 0.665 ± 0.261
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.177TyrAla: 3.177 ± 0.647
0.369TyrCys: 0.369 ± 0.179
2.955TyrAsp: 2.955 ± 0.525
2.512TyrGlu: 2.512 ± 0.518
0.591TyrPhe: 0.591 ± 0.183
3.472TyrGly: 3.472 ± 0.51
0.296TyrHis: 0.296 ± 0.143
1.33TyrIle: 1.33 ± 0.3
2.734TyrLys: 2.734 ± 0.48
2.734TyrLeu: 2.734 ± 0.405
1.773TyrMet: 1.773 ± 0.369
1.921TyrAsn: 1.921 ± 0.463
1.256TyrPro: 1.256 ± 0.356
1.921TyrGln: 1.921 ± 0.317
1.625TyrArg: 1.625 ± 0.355
2.364TyrSer: 2.364 ± 0.39
2.216TyrThr: 2.216 ± 0.383
2.881TyrVal: 2.881 ± 0.522
0.443TyrTrp: 0.443 ± 0.211
1.773TyrTyr: 1.773 ± 0.369
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 68 proteins (13536 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski