Listeria phage B025

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Trabyvirinae; Slepowronvirus; unclassified Slepowronvirus

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A8ATE2|A8ATE2_9CAUD Gp49 OS=Listeria phage B025 OX=330396 PE=4 SV=1
MM1 pKa = 7.27GVSIDD6 pKa = 4.04LYY8 pKa = 10.72SYY10 pKa = 10.74DD11 pKa = 3.69YY12 pKa = 10.32EE13 pKa = 4.67ALVEE17 pKa = 4.91GIQSYY22 pKa = 8.6TKK24 pKa = 10.85AEE26 pKa = 3.92NTEE29 pKa = 4.26VIRR32 pKa = 11.84KK33 pKa = 8.74ILLIGGNVVGDD44 pKa = 3.82KK45 pKa = 11.03YY46 pKa = 10.96IILNNEE52 pKa = 3.32LWEE55 pKa = 5.17DD56 pKa = 3.18NSSYY60 pKa = 11.42YY61 pKa = 10.5NVPNALEE68 pKa = 3.9RR69 pKa = 11.84LYY71 pKa = 11.12KK72 pKa = 10.33VDD74 pKa = 4.82DD75 pKa = 3.74VFGKK79 pKa = 10.17IFCTFDD85 pKa = 3.15DD86 pKa = 3.97RR87 pKa = 11.84FGRR90 pKa = 11.84EE91 pKa = 3.77TLINGCDD98 pKa = 3.39TPEE101 pKa = 4.56EE102 pKa = 3.93ILEE105 pKa = 4.24EE106 pKa = 4.16VMEE109 pKa = 4.41

Molecular weight: 12.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A8ATC5|A8ATC5_9CAUD Gp32 OS=Listeria phage B025 OX=330396 PE=4 SV=1
MM1 pKa = 7.53SLFLSFLRR9 pKa = 11.84NVTSATARR17 pKa = 11.84VIISRR22 pKa = 11.84TVFISSKK29 pKa = 9.36TSFTISTPFYY39 pKa = 8.28IHH41 pKa = 7.2IIIHH45 pKa = 4.96VLYY48 pKa = 10.3KK49 pKa = 10.05VKK51 pKa = 10.35HH52 pKa = 4.81YY53 pKa = 10.24FF54 pKa = 3.37

Molecular weight: 6.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

13083

49

1640

201.3

22.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.535 ± 0.736

0.726 ± 0.139

6.191 ± 0.284

7.529 ± 0.425

4.479 ± 0.343

5.924 ± 0.486

1.666 ± 0.188

7.177 ± 0.315

9.34 ± 0.525

7.926 ± 0.226

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.247 ± 0.123

6.642 ± 0.361

2.431 ± 0.182

3.531 ± 0.212

3.654 ± 0.317

6.405 ± 0.356

5.74 ± 0.337

6.336 ± 0.263

1.284 ± 0.131

4.235 ± 0.32
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski