Atopostipes suicloacalis DSM 15692
Average proteome isoelectric point is 5.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1926 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1M4ZPY5|A0A1M4ZPY5_9LACT Putative pre-16S rRNA nuclease OS=Atopostipes suicloacalis DSM 15692 OX=1121025 GN=SAMN02745249_02017 PE=3 SV=1
MM1 pKa = 7.43 KK2 pKa = 10.3 KK3 pKa = 10.28 IIALLSLTLMSIFILTSCGTNNLGEE28 pKa = 4.03 EE29 pKa = 4.28 SPYY32 pKa = 11.37 GEE34 pKa = 5.06 DD35 pKa = 3.48 VNNLDD40 pKa = 4.09 GVSIEE45 pKa = 4.52 LNKK48 pKa = 9.71 DD49 pKa = 3.23 TYY51 pKa = 11.02 KK52 pKa = 10.87 PEE54 pKa = 4.31 GDD56 pKa = 3.21 IFEE59 pKa = 4.53 LTVINDD65 pKa = 3.56 SEE67 pKa = 4.11 EE68 pKa = 4.64 DD69 pKa = 3.03 ISYY72 pKa = 10.21 GVPYY76 pKa = 9.45 TLEE79 pKa = 3.99 YY80 pKa = 10.62 YY81 pKa = 10.3 KK82 pKa = 10.5 EE83 pKa = 4.31 DD84 pKa = 2.47 KK85 pKa = 10.02 WYY87 pKa = 10.19 EE88 pKa = 3.9 VEE90 pKa = 4.27 PDD92 pKa = 3.39 NEE94 pKa = 4.19 IGFVLILYY102 pKa = 7.33 TLSPGDD108 pKa = 3.67 EE109 pKa = 4.35 ASDD112 pKa = 4.05 EE113 pKa = 4.43 INLDD117 pKa = 3.31 SYY119 pKa = 11.71 EE120 pKa = 4.02 PLEE123 pKa = 4.14 TGRR126 pKa = 11.84 YY127 pKa = 8.41 RR128 pKa = 11.84 VLRR131 pKa = 11.84 QIGDD135 pKa = 3.52 EE136 pKa = 4.08 TLTTEE141 pKa = 4.5 FEE143 pKa = 4.49 VAEE146 pKa = 4.27 EE147 pKa = 4.05
Molecular weight: 16.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.738
IPC2_protein 3.808
IPC_protein 3.757
Toseland 3.567
ProMoST 3.859
Dawson 3.706
Bjellqvist 3.91
Wikipedia 3.592
Rodwell 3.579
Grimsley 3.478
Solomon 3.694
Lehninger 3.656
Nozaki 3.834
DTASelect 3.961
Thurlkill 3.605
EMBOSS 3.617
Sillero 3.859
Patrickios 0.985
IPC_peptide 3.706
IPC2_peptide 3.846
IPC2.peptide.svr19 3.794
Protein with the highest isoelectric point:
>tr|A0A1M4X6A2|A0A1M4X6A2_9LACT Transporter CPA2 family OS=Atopostipes suicloacalis DSM 15692 OX=1121025 GN=SAMN02745249_01361 PE=4 SV=1
MM1 pKa = 7.7 SEE3 pKa = 3.98 GLTYY7 pKa = 10.24 QPKK10 pKa = 8.89 KK11 pKa = 9.86 RR12 pKa = 11.84 KK13 pKa = 8.77 RR14 pKa = 11.84 KK15 pKa = 8.77 KK16 pKa = 8.39 VHH18 pKa = 5.58 GFRR21 pKa = 11.84 KK22 pKa = 10.01 RR23 pKa = 11.84 MSTKK27 pKa = 9.72 NGRR30 pKa = 11.84 RR31 pKa = 11.84 VLKK34 pKa = 9.89 NRR36 pKa = 11.84 RR37 pKa = 11.84 RR38 pKa = 11.84 KK39 pKa = 8.51 GRR41 pKa = 11.84 KK42 pKa = 9.05 RR43 pKa = 11.84 LTHH46 pKa = 6.22
Molecular weight: 5.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.339
IPC2_protein 10.877
IPC_protein 12.281
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.281
Grimsley 12.486
Solomon 12.939
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 12.003
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.005
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1926
0
1926
597593
41
2167
310.3
35.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.447 ± 0.053
0.404 ± 0.012
5.849 ± 0.058
8.643 ± 0.065
4.482 ± 0.047
6.415 ± 0.054
1.907 ± 0.023
8.034 ± 0.055
6.517 ± 0.055
9.625 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.581 ± 0.023
5.049 ± 0.043
3.368 ± 0.028
3.786 ± 0.038
3.891 ± 0.042
5.924 ± 0.037
5.681 ± 0.035
6.658 ± 0.046
0.869 ± 0.018
3.871 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here